Pseudomonas phage Dolphis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G8LJG7|A0A7G8LJG7_9CAUD Uncharacterized protein OS=Pseudomonas phage Dolphis OX=2762288 PE=4 SV=1
MM1 pKa = 7.38 TSIADD6 pKa = 3.57 QEE8 pKa = 4.23 AALAAQIIDD17 pKa = 3.75 YY18 pKa = 10.41 CKK20 pKa = 11.12 ANALSLNGIVAEE32 pKa = 4.55 LEE34 pKa = 3.93 AGQPLDD40 pKa = 3.88 AEE42 pKa = 4.41 RR43 pKa = 11.84 LEE45 pKa = 5.05 HH46 pKa = 6.27 LTTYY50 pKa = 11.0 CDD52 pKa = 3.9 VQCLTLGGLLDD63 pKa = 4.22 KK64 pKa = 11.13 ACEE67 pKa = 3.99 LHH69 pKa = 7.59 DD70 pKa = 4.69 AMQALEE76 pKa = 4.95 AEE78 pKa = 4.4 FEE80 pKa = 4.53 HH81 pKa = 8.0 YY82 pKa = 10.87 NEE84 pKa = 5.06 DD85 pKa = 3.98 LEE87 pKa = 4.31 ASKK90 pKa = 9.79 WAEE93 pKa = 3.91 DD94 pKa = 3.57 MATMRR99 pKa = 11.84 SAEE102 pKa = 4.14 LNYY105 pKa = 10.82 GYY107 pKa = 11.34
Molecular weight: 11.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.899
IPC2_protein 4.062
IPC_protein 3.986
Toseland 3.808
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.821
Grimsley 3.719
Solomon 3.923
Lehninger 3.884
Nozaki 4.062
DTASelect 4.19
Thurlkill 3.834
EMBOSS 3.846
Sillero 4.088
Patrickios 0.477
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|A0A7G8LJH5|A0A7G8LJH5_9CAUD Uncharacterized protein OS=Pseudomonas phage Dolphis OX=2762288 PE=4 SV=1
MM1 pKa = 7.95 KK2 pKa = 10.31 YY3 pKa = 9.7 QATLRR8 pKa = 11.84 IRR10 pKa = 11.84 SPYY13 pKa = 9.1 EE14 pKa = 3.13 RR15 pKa = 11.84 AGEE18 pKa = 3.86 YY19 pKa = 9.85 RR20 pKa = 11.84 RR21 pKa = 11.84 PSVRR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 10.2 ALTKK31 pKa = 10.68 AEE33 pKa = 3.94 AQALRR38 pKa = 11.84 EE39 pKa = 4.32 ACTVPPIRR47 pKa = 11.84 WAEE50 pKa = 3.84 ATLQRR55 pKa = 11.84 LPEE58 pKa = 4.41 PPSPTFRR65 pKa = 11.84 ASIEE69 pKa = 3.96 QLHH72 pKa = 6.46 AKK74 pKa = 9.66 PGQLCQQVRR83 pKa = 11.84 PATPAAPPTVPTPVPAEE100 pKa = 4.34 DD101 pKa = 3.9 NPAPSVPRR109 pKa = 11.84 VSSPRR114 pKa = 11.84 PKK116 pKa = 10.28 TKK118 pKa = 10.66 LSDD121 pKa = 3.42 LAQATLEE128 pKa = 4.15 RR129 pKa = 11.84 FRR131 pKa = 11.84 ARR133 pKa = 11.84 YY134 pKa = 9.19 ALL136 pKa = 3.8
Molecular weight: 15.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.765
IPC_protein 10.774
Toseland 10.818
ProMoST 10.672
Dawson 10.906
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 10.994
Grimsley 10.95
Solomon 11.096
Lehninger 11.038
Nozaki 10.804
DTASelect 10.672
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.847
Patrickios 10.716
IPC_peptide 11.096
IPC2_peptide 9.853
IPC2.peptide.svr19 8.508
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
124
0
124
28373
39
1222
228.8
25.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.345 ± 0.393
1.174 ± 0.117
5.946 ± 0.224
6.358 ± 0.212
3.123 ± 0.12
8.198 ± 0.254
2.164 ± 0.14
4.638 ± 0.154
4.955 ± 0.295
7.662 ± 0.158
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.125
3.588 ± 0.131
5.213 ± 0.193
4.487 ± 0.18
6.291 ± 0.304
5.731 ± 0.191
5.385 ± 0.183
6.982 ± 0.241
1.678 ± 0.105
2.58 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here