Pseudomonas phage Dolphis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A7G8LJG7|A0A7G8LJG7_9CAUD Uncharacterized protein OS=Pseudomonas phage Dolphis OX=2762288 PE=4 SV=1
MM1 pKa = 7.38TSIADD6 pKa = 3.57QEE8 pKa = 4.23AALAAQIIDD17 pKa = 3.75YY18 pKa = 10.41CKK20 pKa = 11.12ANALSLNGIVAEE32 pKa = 4.55LEE34 pKa = 3.93AGQPLDD40 pKa = 3.88AEE42 pKa = 4.41RR43 pKa = 11.84LEE45 pKa = 5.05HH46 pKa = 6.27LTTYY50 pKa = 11.0CDD52 pKa = 3.9VQCLTLGGLLDD63 pKa = 4.22KK64 pKa = 11.13ACEE67 pKa = 3.99LHH69 pKa = 7.59DD70 pKa = 4.69AMQALEE76 pKa = 4.95AEE78 pKa = 4.4FEE80 pKa = 4.53HH81 pKa = 8.0YY82 pKa = 10.87NEE84 pKa = 5.06DD85 pKa = 3.98LEE87 pKa = 4.31ASKK90 pKa = 9.79WAEE93 pKa = 3.91DD94 pKa = 3.57MATMRR99 pKa = 11.84SAEE102 pKa = 4.14LNYY105 pKa = 10.82GYY107 pKa = 11.34

Molecular weight:
11.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A7G8LJH5|A0A7G8LJH5_9CAUD Uncharacterized protein OS=Pseudomonas phage Dolphis OX=2762288 PE=4 SV=1
MM1 pKa = 7.95KK2 pKa = 10.31YY3 pKa = 9.7QATLRR8 pKa = 11.84IRR10 pKa = 11.84SPYY13 pKa = 9.1EE14 pKa = 3.13RR15 pKa = 11.84AGEE18 pKa = 3.86YY19 pKa = 9.85RR20 pKa = 11.84RR21 pKa = 11.84PSVRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 10.2ALTKK31 pKa = 10.68AEE33 pKa = 3.94AQALRR38 pKa = 11.84EE39 pKa = 4.32ACTVPPIRR47 pKa = 11.84WAEE50 pKa = 3.84ATLQRR55 pKa = 11.84LPEE58 pKa = 4.41PPSPTFRR65 pKa = 11.84ASIEE69 pKa = 3.96QLHH72 pKa = 6.46AKK74 pKa = 9.66PGQLCQQVRR83 pKa = 11.84PATPAAPPTVPTPVPAEE100 pKa = 4.34DD101 pKa = 3.9NPAPSVPRR109 pKa = 11.84VSSPRR114 pKa = 11.84PKK116 pKa = 10.28TKK118 pKa = 10.66LSDD121 pKa = 3.42LAQATLEE128 pKa = 4.15RR129 pKa = 11.84FRR131 pKa = 11.84ARR133 pKa = 11.84YY134 pKa = 9.19ALL136 pKa = 3.8

Molecular weight:
15.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

124

0

124

28373

39

1222

228.8

25.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.345 ± 0.393

1.174 ± 0.117

5.946 ± 0.224

6.358 ± 0.212

3.123 ± 0.12

8.198 ± 0.254

2.164 ± 0.14

4.638 ± 0.154

4.955 ± 0.295

7.662 ± 0.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.125

3.588 ± 0.131

5.213 ± 0.193

4.487 ± 0.18

6.291 ± 0.304

5.731 ± 0.191

5.385 ± 0.183

6.982 ± 0.241

1.678 ± 0.105

2.58 ± 0.12

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski