Mycobacterium phage Catera
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q19XE3|Q19XE3_9CAUD Uncharacterized protein OS=Mycobacterium phage Catera OX=373404 GN=124 PE=4 SV=1
MM1 pKa = 7.81 ADD3 pKa = 3.58 YY4 pKa = 11.13 ASDD7 pKa = 4.1 CPISRR12 pKa = 11.84 PVDD15 pKa = 3.83 PGWTSDD21 pKa = 2.87 WDD23 pKa = 3.89 LGGMQWSYY31 pKa = 11.65 DD32 pKa = 3.36 YY33 pKa = 11.9 GMFYY37 pKa = 10.44 QPSPDD42 pKa = 3.22 EE43 pKa = 4.38 LLPLRR48 pKa = 11.84 KK49 pKa = 8.64 PAQRR53 pKa = 11.84 VGEE56 pKa = 4.11
Molecular weight: 6.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 4.012
IPC_protein 3.897
Toseland 3.681
ProMoST 4.024
Dawson 3.923
Bjellqvist 4.19
Wikipedia 3.91
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.846
Nozaki 4.062
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|Q19XL7|Q19XL7_9CAUD Uncharacterized protein OS=Mycobacterium phage Catera OX=373404 GN=45 PE=4 SV=1
MM1 pKa = 7.8 ALPALTWRR9 pKa = 11.84 TEE11 pKa = 3.84 TNGRR15 pKa = 11.84 KK16 pKa = 9.09 KK17 pKa = 10.65 VATTHH22 pKa = 6.57 LRR24 pKa = 11.84 TTYY27 pKa = 9.89 TLEE30 pKa = 5.16 KK31 pKa = 9.93 IDD33 pKa = 4.01 GTWVARR39 pKa = 11.84 VTVPQTRR46 pKa = 11.84 NRR48 pKa = 11.84 IGGGTEE54 pKa = 3.94 TLVSGVTFAQARR66 pKa = 11.84 AAALDD71 pKa = 3.79 DD72 pKa = 3.8 YY73 pKa = 11.21 KK74 pKa = 11.41 RR75 pKa = 11.84 NGGNN79 pKa = 3.07
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.76
ProMoST 10.482
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.052
Grimsley 10.906
Solomon 10.994
Lehninger 10.965
Nozaki 10.73
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.847
IPC_peptide 10.994
IPC2_peptide 9.355
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
218
0
218
46211
28
1369
212.0
23.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.783 ± 0.26
0.818 ± 0.074
7.115 ± 0.17
6.364 ± 0.164
3.138 ± 0.091
8.095 ± 0.261
2.46 ± 0.139
4.328 ± 0.133
3.819 ± 0.133
7.561 ± 0.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.105
3.391 ± 0.112
5.654 ± 0.174
4.153 ± 0.142
6.84 ± 0.181
5.436 ± 0.14
6.464 ± 0.204
7.117 ± 0.192
1.906 ± 0.089
3.053 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here