Amino acid dipepetide frequency for Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.343AlaAla: 10.343 ± 0.206	1.199AlaCys: 1.199 ± 0.045	4.672AlaAsp: 4.672 ± 0.106	5.313AlaGlu: 5.313 ± 0.108	3.438AlaPhe: 3.438 ± 0.083	8.11AlaGly: 8.11 ± 0.148	1.68AlaHis: 1.68 ± 0.052	5.241AlaIle: 5.241 ± 0.104	4.617AlaLys: 4.617 ± 0.111	9.855AlaLeu: 9.855 ± 0.155	2.78AlaMet: 2.78 ± 0.073	2.702AlaAsn: 2.702 ± 0.116	3.21AlaPro: 3.21 ± 0.096	3.314AlaGln: 3.314 ± 0.078	5.396AlaArg: 5.396 ± 0.118	4.287AlaSer: 4.287 ± 0.114	3.715AlaThr: 3.715 ± 0.106	7.045AlaVal: 7.045 ± 0.139	0.859AlaTrp: 0.859 ± 0.039	2.602AlaTyr: 2.602 ± 0.067	0.0AlaXaa: 0.0 ± 0.0
Cys	1.054CysAla: 1.054 ± 0.041	0.269CysCys: 0.269 ± 0.024	0.596CysAsp: 0.596 ± 0.031	0.666CysGlu: 0.666 ± 0.035	0.563CysPhe: 0.563 ± 0.034	1.441CysGly: 1.441 ± 0.049	0.348CysHis: 0.348 ± 0.023	0.747CysIle: 0.747 ± 0.035	0.485CysLys: 0.485 ± 0.028	1.408CysLeu: 1.408 ± 0.057	0.294CysMet: 0.294 ± 0.023	0.387CysAsn: 0.387 ± 0.029	0.717CysPro: 0.717 ± 0.041	0.462CysGln: 0.462 ± 0.027	0.889CysArg: 0.889 ± 0.044	0.732CysSer: 0.732 ± 0.034	0.695CysThr: 0.695 ± 0.035	0.821CysVal: 0.821 ± 0.038	0.157CysTrp: 0.157 ± 0.016	0.403CysTyr: 0.403 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.566AspAla: 4.566 ± 0.105	0.675AspCys: 0.675 ± 0.033	2.303AspAsp: 2.303 ± 0.069	3.198AspGlu: 3.198 ± 0.078	2.461AspPhe: 2.461 ± 0.073	4.363AspGly: 4.363 ± 0.128	1.279AspHis: 1.279 ± 0.041	3.175AspIle: 3.175 ± 0.072	3.102AspLys: 3.102 ± 0.082	5.577AspLeu: 5.577 ± 0.093	1.574AspMet: 1.574 ± 0.047	1.789AspAsn: 1.789 ± 0.078	2.832AspPro: 2.832 ± 0.073	1.879AspGln: 1.879 ± 0.056	3.196AspArg: 3.196 ± 0.086	2.514AspSer: 2.514 ± 0.07	2.932AspThr: 2.932 ± 0.095	3.834AspVal: 3.834 ± 0.087	0.695AspTrp: 0.695 ± 0.032	2.0AspTyr: 2.0 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	6.351GluAla: 6.351 ± 0.115	0.544GluCys: 0.544 ± 0.031	3.564GluAsp: 3.564 ± 0.087	5.728GluGlu: 5.728 ± 0.127	1.939GluPhe: 1.939 ± 0.056	4.824GluGly: 4.824 ± 0.099	1.141GluHis: 1.141 ± 0.044	3.924GluIle: 3.924 ± 0.096	5.949GluLys: 5.949 ± 0.101	5.815GluLeu: 5.815 ± 0.1	2.101GluMet: 2.101 ± 0.067	2.787GluAsn: 2.787 ± 0.069	2.224GluPro: 2.224 ± 0.065	2.76GluGln: 2.76 ± 0.072	3.128GluArg: 3.128 ± 0.077	2.418GluSer: 2.418 ± 0.055	3.486GluThr: 3.486 ± 0.076	4.097GluVal: 4.097 ± 0.079	0.542GluTrp: 0.542 ± 0.028	1.895GluTyr: 1.895 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	3.441PheAla: 3.441 ± 0.085	0.678PheCys: 0.678 ± 0.031	2.136PheAsp: 2.136 ± 0.057	1.603PheGlu: 1.603 ± 0.049	1.773PhePhe: 1.773 ± 0.068	3.238PheGly: 3.238 ± 0.08	0.962PheHis: 0.962 ± 0.037	2.127PheIle: 2.127 ± 0.065	1.514PheLys: 1.514 ± 0.055	4.487PheLeu: 4.487 ± 0.118	1.053PheMet: 1.053 ± 0.04	1.296PheAsn: 1.296 ± 0.04	1.528PhePro: 1.528 ± 0.046	1.543PheGln: 1.543 ± 0.049	2.075PheArg: 2.075 ± 0.062	2.349PheSer: 2.349 ± 0.076	2.37PheThr: 2.37 ± 0.069	2.69PheVal: 2.69 ± 0.069	0.476PheTrp: 0.476 ± 0.026	1.299PheTyr: 1.299 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	6.906GlyAla: 6.906 ± 0.149	1.338GlyCys: 1.338 ± 0.055	4.01GlyAsp: 4.01 ± 0.094	4.612GlyGlu: 4.612 ± 0.093	3.307GlyPhe: 3.307 ± 0.079	6.067GlyGly: 6.067 ± 0.134	1.671GlyHis: 1.671 ± 0.052	5.559GlyIle: 5.559 ± 0.124	5.947GlyLys: 5.947 ± 0.11	7.661GlyLeu: 7.661 ± 0.138	2.559GlyMet: 2.559 ± 0.069	3.022GlyAsn: 3.022 ± 0.109	2.361GlyPro: 2.361 ± 0.063	2.679GlyGln: 2.679 ± 0.067	4.21GlyArg: 4.21 ± 0.09	4.75GlySer: 4.75 ± 0.155	5.205GlyThr: 5.205 ± 0.148	5.944GlyVal: 5.944 ± 0.098	0.907GlyTrp: 0.907 ± 0.036	2.929GlyTyr: 2.929 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.371HisAla: 1.371 ± 0.048	0.378HisCys: 0.378 ± 0.023	0.954HisAsp: 0.954 ± 0.038	0.922HisGlu: 0.922 ± 0.037	0.893HisPhe: 0.893 ± 0.037	1.665HisGly: 1.665 ± 0.055	0.562HisHis: 0.562 ± 0.033	1.25HisIle: 1.25 ± 0.044	0.995HisLys: 0.995 ± 0.042	1.985HisLeu: 1.985 ± 0.066	0.63HisMet: 0.63 ± 0.033	0.717HisAsn: 0.717 ± 0.031	1.365HisPro: 1.365 ± 0.047	0.808HisGln: 0.808 ± 0.042	1.11HisArg: 1.11 ± 0.045	1.05HisSer: 1.05 ± 0.045	1.207HisThr: 1.207 ± 0.042	1.328HisVal: 1.328 ± 0.045	0.288HisTrp: 0.288 ± 0.023	0.769HisTyr: 0.769 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	5.062IleAla: 5.062 ± 0.101	0.871IleCys: 0.871 ± 0.042	2.872IleAsp: 2.872 ± 0.074	2.563IleGlu: 2.563 ± 0.064	2.16IlePhe: 2.16 ± 0.064	4.691IleGly: 4.691 ± 0.117	1.394IleHis: 1.394 ± 0.049	3.419IleIle: 3.419 ± 0.102	2.448IleLys: 2.448 ± 0.075	6.509IleLeu: 6.509 ± 0.121	1.429IleMet: 1.429 ± 0.057	2.043IleAsn: 2.043 ± 0.072	3.166IlePro: 3.166 ± 0.078	2.275IleGln: 2.275 ± 0.07	3.852IleArg: 3.852 ± 0.078	3.523IleSer: 3.523 ± 0.089	3.64IleThr: 3.64 ± 0.092	3.957IleVal: 3.957 ± 0.091	0.509IleTrp: 0.509 ± 0.027	1.792IleTyr: 1.792 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	5.756LysAla: 5.756 ± 0.124	0.465LysCys: 0.465 ± 0.027	3.519LysAsp: 3.519 ± 0.083	4.915LysGlu: 4.915 ± 0.104	1.667LysPhe: 1.667 ± 0.053	4.491LysGly: 4.491 ± 0.084	0.818LysHis: 0.818 ± 0.036	3.804LysIle: 3.804 ± 0.08	4.995LysLys: 4.995 ± 0.113	5.02LysLeu: 5.02 ± 0.095	2.085LysMet: 2.085 ± 0.066	2.57LysAsn: 2.57 ± 0.081	2.187LysPro: 2.187 ± 0.069	2.006LysGln: 2.006 ± 0.055	2.639LysArg: 2.639 ± 0.067	2.487LysSer: 2.487 ± 0.067	3.271LysThr: 3.271 ± 0.082	4.385LysVal: 4.385 ± 0.099	0.5LysTrp: 0.5 ± 0.027	1.916LysTyr: 1.916 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	9.897LeuAla: 9.897 ± 0.144	1.507LeuCys: 1.507 ± 0.049	5.955LeuAsp: 5.955 ± 0.102	6.878LeuGlu: 6.878 ± 0.137	4.048LeuPhe: 4.048 ± 0.114	8.239LeuGly: 8.239 ± 0.13	1.994LeuHis: 1.994 ± 0.06	4.915LeuIle: 4.915 ± 0.105	5.774LeuLys: 5.774 ± 0.095	10.279LeuLeu: 10.279 ± 0.217	2.656LeuMet: 2.656 ± 0.081	3.05LeuAsn: 3.05 ± 0.076	4.881LeuPro: 4.881 ± 0.108	3.825LeuGln: 3.825 ± 0.091	4.887LeuArg: 4.887 ± 0.108	5.774LeuSer: 5.774 ± 0.118	5.819LeuThr: 5.819 ± 0.113	7.199LeuVal: 7.199 ± 0.129	0.95LeuTrp: 0.95 ± 0.05	2.983LeuTyr: 2.983 ± 0.079	0.0LeuXaa: 0.0 ± 0.0
Met	3.01MetAla: 3.01 ± 0.078	0.251MetCys: 0.251 ± 0.019	1.934MetAsp: 1.934 ± 0.058	2.282MetGlu: 2.282 ± 0.064	0.856MetPhe: 0.856 ± 0.044	2.484MetGly: 2.484 ± 0.064	0.499MetHis: 0.499 ± 0.028	1.589MetIle: 1.589 ± 0.053	2.061MetLys: 2.061 ± 0.061	2.596MetLeu: 2.596 ± 0.075	0.81MetMet: 0.81 ± 0.035	1.141MetAsn: 1.141 ± 0.044	1.208MetPro: 1.208 ± 0.038	0.757MetGln: 0.757 ± 0.039	1.114MetArg: 1.114 ± 0.046	1.383MetSer: 1.383 ± 0.044	1.795MetThr: 1.795 ± 0.063	2.13MetVal: 2.13 ± 0.059	0.193MetTrp: 0.193 ± 0.018	0.606MetTyr: 0.606 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	2.809AsnAla: 2.809 ± 0.089	0.47AsnCys: 0.47 ± 0.028	1.685AsnAsp: 1.685 ± 0.066	1.739AsnGlu: 1.739 ± 0.067	1.29AsnPhe: 1.29 ± 0.044	3.145AsnGly: 3.145 ± 0.118	0.759AsnHis: 0.759 ± 0.037	2.33AsnIle: 2.33 ± 0.072	1.91AsnLys: 1.91 ± 0.075	3.75AsnLeu: 3.75 ± 0.082	1.066AsnMet: 1.066 ± 0.05	1.22AsnAsn: 1.22 ± 0.072	2.127AsnPro: 2.127 ± 0.056	1.299AsnGln: 1.299 ± 0.052	2.06AsnArg: 2.06 ± 0.059	1.532AsnSer: 1.532 ± 0.063	1.874AsnThr: 1.874 ± 0.09	2.569AsnVal: 2.569 ± 0.077	0.418AsnTrp: 0.418 ± 0.025	1.229AsnTyr: 1.229 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.797ProAla: 3.797 ± 0.098	0.496ProCys: 0.496 ± 0.028	2.7ProAsp: 2.7 ± 0.071	4.346ProGlu: 4.346 ± 0.097	1.845ProPhe: 1.845 ± 0.057	3.661ProGly: 3.661 ± 0.083	0.916ProHis: 0.916 ± 0.043	2.103ProIle: 2.103 ± 0.07	1.88ProLys: 1.88 ± 0.057	4.054ProLeu: 4.054 ± 0.108	1.108ProMet: 1.108 ± 0.046	1.249ProAsn: 1.249 ± 0.039	1.202ProPro: 1.202 ± 0.05	1.522ProGln: 1.522 ± 0.044	2.06ProArg: 2.06 ± 0.061	1.96ProSer: 1.96 ± 0.056	1.777ProThr: 1.777 ± 0.06	3.84ProVal: 3.84 ± 0.067	0.502ProTrp: 0.502 ± 0.032	1.534ProTyr: 1.534 ± 0.056	0.0ProXaa: 0.0 ± 0.0
Gln	3.643GlnAla: 3.643 ± 0.088	0.402GlnCys: 0.402 ± 0.027	2.027GlnAsp: 2.027 ± 0.058	3.06GlnGlu: 3.06 ± 0.085	1.208GlnPhe: 1.208 ± 0.043	2.918GlnGly: 2.918 ± 0.069	0.642GlnHis: 0.642 ± 0.035	1.9GlnIle: 1.9 ± 0.051	2.56GlnLys: 2.56 ± 0.063	3.579GlnLeu: 3.579 ± 0.084	1.032GlnMet: 1.032 ± 0.037	1.344GlnAsn: 1.344 ± 0.054	1.552GlnPro: 1.552 ± 0.053	1.612GlnGln: 1.612 ± 0.069	1.595GlnArg: 1.595 ± 0.053	1.5GlnSer: 1.5 ± 0.049	1.483GlnThr: 1.483 ± 0.042	3.169GlnVal: 3.169 ± 0.073	0.467GlnTrp: 0.467 ± 0.027	1.274GlnTyr: 1.274 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	3.813ArgAla: 3.813 ± 0.092	0.645ArgCys: 0.645 ± 0.035	2.945ArgAsp: 2.945 ± 0.069	4.512ArgGlu: 4.512 ± 0.108	2.061ArgPhe: 2.061 ± 0.06	3.246ArgGly: 3.246 ± 0.073	1.013ArgHis: 1.013 ± 0.038	3.349ArgIle: 3.349 ± 0.062	4.073ArgLys: 4.073 ± 0.091	5.105ArgLeu: 5.105 ± 0.12	1.494ArgMet: 1.494 ± 0.047	2.084ArgAsn: 2.084 ± 0.061	2.13ArgPro: 2.13 ± 0.057	2.599ArgGln: 2.599 ± 0.079	2.845ArgArg: 2.845 ± 0.08	2.524ArgSer: 2.524 ± 0.065	2.811ArgThr: 2.811 ± 0.068	3.377ArgVal: 3.377 ± 0.082	0.572ArgTrp: 0.572 ± 0.033	2.0ArgTyr: 2.0 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	3.986SerAla: 3.986 ± 0.094	0.695SerCys: 0.695 ± 0.036	2.426SerAsp: 2.426 ± 0.082	2.302SerGlu: 2.302 ± 0.061	2.234SerPhe: 2.234 ± 0.066	4.782SerGly: 4.782 ± 0.112	1.225SerHis: 1.225 ± 0.043	3.042SerIle: 3.042 ± 0.083	2.279SerLys: 2.279 ± 0.058	5.711SerLeu: 5.711 ± 0.107	1.398SerMet: 1.398 ± 0.046	1.641SerAsn: 1.641 ± 0.066	1.994SerPro: 1.994 ± 0.056	2.013SerGln: 2.013 ± 0.07	3.153SerArg: 3.153 ± 0.077	2.78SerSer: 2.78 ± 0.089	2.792SerThr: 2.792 ± 0.088	3.64SerVal: 3.64 ± 0.101	0.617SerTrp: 0.617 ± 0.029	1.727SerTyr: 1.727 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	5.139ThrAla: 5.139 ± 0.152	0.665ThrCys: 0.665 ± 0.033	2.936ThrAsp: 2.936 ± 0.093	3.093ThrGlu: 3.093 ± 0.075	2.069ThrPhe: 2.069 ± 0.057	5.277ThrGly: 5.277 ± 0.118	1.047ThrHis: 1.047 ± 0.039	3.374ThrIle: 3.374 ± 0.086	2.331ThrLys: 2.331 ± 0.068	5.989ThrLeu: 5.989 ± 0.111	1.371ThrMet: 1.371 ± 0.042	1.813ThrAsn: 1.813 ± 0.096	2.566ThrPro: 2.566 ± 0.061	1.482ThrGln: 1.482 ± 0.054	2.826ThrArg: 2.826 ± 0.065	2.624ThrSer: 2.624 ± 0.106	2.826ThrThr: 2.826 ± 0.143	4.814ThrVal: 4.814 ± 0.133	0.605ThrTrp: 0.605 ± 0.031	1.624ThrTyr: 1.624 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	6.028ValAla: 6.028 ± 0.112	1.002ValCys: 1.002 ± 0.043	4.197ValAsp: 4.197 ± 0.086	4.854ValGlu: 4.854 ± 0.093	2.872ValPhe: 2.872 ± 0.068	5.235ValGly: 5.235 ± 0.101	1.244ValHis: 1.244 ± 0.046	4.13ValIle: 4.13 ± 0.091	4.281ValLys: 4.281 ± 0.093	7.805ValLeu: 7.805 ± 0.14	2.109ValMet: 2.109 ± 0.062	2.685ValAsn: 2.685 ± 0.083	3.526ValPro: 3.526 ± 0.094	2.222ValGln: 2.222 ± 0.067	3.774ValArg: 3.774 ± 0.072	4.143ValSer: 4.143 ± 0.091	4.491ValThr: 4.491 ± 0.15	5.425ValVal: 5.425 ± 0.109	0.683ValTrp: 0.683 ± 0.034	2.382ValTyr: 2.382 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.698TrpAla: 0.698 ± 0.036	0.136TrpCys: 0.136 ± 0.013	0.596TrpAsp: 0.596 ± 0.03	0.693TrpGlu: 0.693 ± 0.037	0.402TrpPhe: 0.402 ± 0.027	0.772TrpGly: 0.772 ± 0.04	0.257TrpHis: 0.257 ± 0.019	0.577TrpIle: 0.577 ± 0.033	0.778TrpLys: 0.778 ± 0.034	1.089TrpLeu: 1.089 ± 0.047	0.311TrpMet: 0.311 ± 0.025	0.475TrpAsn: 0.475 ± 0.029	0.384TrpPro: 0.384 ± 0.024	0.594TrpGln: 0.594 ± 0.031	0.491TrpArg: 0.491 ± 0.03	0.511TrpSer: 0.511 ± 0.028	0.494TrpThr: 0.494 ± 0.026	0.666TrpVal: 0.666 ± 0.031	0.1TrpTrp: 0.1 ± 0.013	0.344TrpTyr: 0.344 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.706TyrAla: 2.706 ± 0.061	0.45TyrCys: 0.45 ± 0.027	1.916TyrAsp: 1.916 ± 0.066	1.937TyrGlu: 1.937 ± 0.056	1.553TyrPhe: 1.553 ± 0.051	2.995TyrGly: 2.995 ± 0.071	0.798TyrHis: 0.798 ± 0.034	1.622TyrIle: 1.622 ± 0.057	1.516TyrLys: 1.516 ± 0.042	3.235TyrLeu: 3.235 ± 0.069	0.838TyrMet: 0.838 ± 0.034	1.268TyrAsn: 1.268 ± 0.042	1.391TyrPro: 1.391 ± 0.043	1.316TyrGln: 1.316 ± 0.041	1.897TyrArg: 1.897 ± 0.059	1.597TyrSer: 1.597 ± 0.049	1.788TyrThr: 1.788 ± 0.062	2.16TyrVal: 2.16 ± 0.055	0.327TyrTrp: 0.327 ± 0.023	1.243TyrTyr: 1.243 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2016 proteins (669530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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