Amino acid dipepetide frequency for Escherichia phage ECML-117

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.824AlaAla: 9.824 ± 1.136
0.496AlaCys: 0.496 ± 0.166
5.11AlaAsp: 5.11 ± 0.477
5.755AlaGlu: 5.755 ± 0.952
3.523AlaPhe: 3.523 ± 0.49
6.897AlaGly: 6.897 ± 0.771
1.588AlaHis: 1.588 ± 0.301
5.309AlaIle: 5.309 ± 0.469
5.656AlaLys: 5.656 ± 1.164
6.549AlaLeu: 6.549 ± 0.496
2.679AlaMet: 2.679 ± 0.342
3.87AlaAsn: 3.87 ± 0.661
2.679AlaPro: 2.679 ± 0.35
3.027AlaGln: 3.027 ± 0.508
3.771AlaArg: 3.771 ± 0.455
5.011AlaSer: 5.011 ± 0.546
5.507AlaThr: 5.507 ± 0.612
6.003AlaVal: 6.003 ± 0.653
1.191AlaTrp: 1.191 ± 0.232
2.729AlaTyr: 2.729 ± 0.305
0.0AlaXaa: 0.0 ± 0.0
Cys
0.893CysAla: 0.893 ± 0.235
0.099CysCys: 0.099 ± 0.063
0.595CysAsp: 0.595 ± 0.191
0.546CysGlu: 0.546 ± 0.146
0.347CysPhe: 0.347 ± 0.144
0.695CysGly: 0.695 ± 0.18
0.248CysHis: 0.248 ± 0.107
0.546CysIle: 0.546 ± 0.165
0.397CysLys: 0.397 ± 0.151
0.645CysLeu: 0.645 ± 0.186
0.248CysMet: 0.248 ± 0.099
0.397CysAsn: 0.397 ± 0.147
0.595CysPro: 0.595 ± 0.147
0.496CysGln: 0.496 ± 0.129
0.447CysArg: 0.447 ± 0.186
0.447CysSer: 0.447 ± 0.149
0.546CysThr: 0.546 ± 0.156
0.645CysVal: 0.645 ± 0.172
0.099CysTrp: 0.099 ± 0.076
0.397CysTyr: 0.397 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
5.011AspAla: 5.011 ± 0.683
0.695AspCys: 0.695 ± 0.226
3.969AspAsp: 3.969 ± 0.493
4.317AspGlu: 4.317 ± 0.573
2.58AspPhe: 2.58 ± 0.313
5.408AspGly: 5.408 ± 0.596
0.943AspHis: 0.943 ± 0.204
3.622AspIle: 3.622 ± 0.417
3.175AspLys: 3.175 ± 0.413
4.515AspLeu: 4.515 ± 0.5
1.191AspMet: 1.191 ± 0.255
2.679AspAsn: 2.679 ± 0.33
2.63AspPro: 2.63 ± 0.353
2.431AspGln: 2.431 ± 0.32
2.034AspArg: 2.034 ± 0.335
3.275AspSer: 3.275 ± 0.507
2.778AspThr: 2.778 ± 0.414
5.061AspVal: 5.061 ± 0.502
1.191AspTrp: 1.191 ± 0.387
2.332AspTyr: 2.332 ± 0.403
0.0AspXaa: 0.0 ± 0.0
Glu
5.656GluAla: 5.656 ± 0.77
0.347GluCys: 0.347 ± 0.147
3.721GluAsp: 3.721 ± 0.562
5.11GluGlu: 5.11 ± 0.757
3.126GluPhe: 3.126 ± 0.39
4.317GluGly: 4.317 ± 0.499
1.24GluHis: 1.24 ± 0.232
4.565GluIle: 4.565 ± 0.575
3.572GluLys: 3.572 ± 0.653
5.21GluLeu: 5.21 ± 0.478
2.133GluMet: 2.133 ± 0.349
2.828GluAsn: 2.828 ± 0.361
1.637GluPro: 1.637 ± 0.309
2.382GluGln: 2.382 ± 0.48
4.317GluArg: 4.317 ± 0.567
3.92GluSer: 3.92 ± 0.497
3.076GluThr: 3.076 ± 0.291
4.763GluVal: 4.763 ± 0.584
1.24GluTrp: 1.24 ± 0.258
2.729GluTyr: 2.729 ± 0.362
0.0GluXaa: 0.0 ± 0.0
Phe
2.927PheAla: 2.927 ± 0.341
0.298PheCys: 0.298 ± 0.116
3.423PheAsp: 3.423 ± 0.527
2.927PheGlu: 2.927 ± 0.292
1.34PhePhe: 1.34 ± 0.279
3.672PheGly: 3.672 ± 0.411
0.347PheHis: 0.347 ± 0.121
2.58PheIle: 2.58 ± 0.352
3.175PheLys: 3.175 ± 0.365
2.878PheLeu: 2.878 ± 0.397
0.794PheMet: 0.794 ± 0.196
2.679PheAsn: 2.679 ± 0.354
1.389PhePro: 1.389 ± 0.252
1.191PheGln: 1.191 ± 0.26
1.538PheArg: 1.538 ± 0.308
1.836PheSer: 1.836 ± 0.302
1.885PheThr: 1.885 ± 0.387
3.523PheVal: 3.523 ± 0.429
0.298PheTrp: 0.298 ± 0.115
1.34PheTyr: 1.34 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
6.549GlyAla: 6.549 ± 0.743
0.943GlyCys: 0.943 ± 0.259
4.912GlyAsp: 4.912 ± 0.47
5.607GlyGlu: 5.607 ± 0.566
2.828GlyPhe: 2.828 ± 0.352
5.16GlyGly: 5.16 ± 0.68
0.794GlyHis: 0.794 ± 0.231
5.358GlyIle: 5.358 ± 0.622
4.118GlyLys: 4.118 ± 0.441
4.912GlyLeu: 4.912 ± 0.482
2.332GlyMet: 2.332 ± 0.302
3.523GlyAsn: 3.523 ± 0.465
1.588GlyPro: 1.588 ± 0.418
2.927GlyGln: 2.927 ± 0.462
4.068GlyArg: 4.068 ± 0.419
4.565GlySer: 4.565 ± 0.573
4.416GlyThr: 4.416 ± 0.788
6.45GlyVal: 6.45 ± 0.64
1.439GlyTrp: 1.439 ± 0.215
2.679GlyTyr: 2.679 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
0.645HisAla: 0.645 ± 0.155
0.397HisCys: 0.397 ± 0.12
1.092HisAsp: 1.092 ± 0.233
1.24HisGlu: 1.24 ± 0.227
0.744HisPhe: 0.744 ± 0.179
1.34HisGly: 1.34 ± 0.272
0.298HisHis: 0.298 ± 0.13
1.042HisIle: 1.042 ± 0.223
0.744HisLys: 0.744 ± 0.218
1.042HisLeu: 1.042 ± 0.252
0.447HisMet: 0.447 ± 0.16
0.992HisAsn: 0.992 ± 0.298
0.595HisPro: 0.595 ± 0.175
0.794HisGln: 0.794 ± 0.177
0.695HisArg: 0.695 ± 0.177
0.943HisSer: 0.943 ± 0.204
0.595HisThr: 0.595 ± 0.201
1.141HisVal: 1.141 ± 0.235
0.149HisTrp: 0.149 ± 0.079
0.794HisTyr: 0.794 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.309IleAla: 5.309 ± 0.568
0.744IleCys: 0.744 ± 0.248
5.259IleAsp: 5.259 ± 0.521
4.416IleGlu: 4.416 ± 0.494
1.985IlePhe: 1.985 ± 0.279
4.317IleGly: 4.317 ± 0.359
0.992IleHis: 0.992 ± 0.254
3.076IleIle: 3.076 ± 0.452
5.706IleLys: 5.706 ± 0.571
4.168IleLeu: 4.168 ± 0.411
1.836IleMet: 1.836 ± 0.287
3.622IleAsn: 3.622 ± 0.384
3.126IlePro: 3.126 ± 0.301
2.53IleGln: 2.53 ± 0.32
2.53IleArg: 2.53 ± 0.313
3.771IleSer: 3.771 ± 0.464
4.267IleThr: 4.267 ± 0.647
4.416IleVal: 4.416 ± 0.428
0.496IleTrp: 0.496 ± 0.182
1.588IleTyr: 1.588 ± 0.234
0.0IleXaa: 0.0 ± 0.0
Lys
6.648LysAla: 6.648 ± 1.092
0.546LysCys: 0.546 ± 0.159
3.92LysAsp: 3.92 ± 0.523
4.763LysGlu: 4.763 ± 0.912
2.729LysPhe: 2.729 ± 0.364
4.614LysGly: 4.614 ± 0.592
0.893LysHis: 0.893 ± 0.229
3.523LysIle: 3.523 ± 0.467
3.87LysLys: 3.87 ± 0.643
4.416LysLeu: 4.416 ± 0.63
2.63LysMet: 2.63 ± 0.379
2.927LysAsn: 2.927 ± 0.311
2.034LysPro: 2.034 ± 0.34
2.233LysGln: 2.233 ± 0.337
4.019LysArg: 4.019 ± 0.588
4.217LysSer: 4.217 ± 0.475
3.126LysThr: 3.126 ± 0.39
4.267LysVal: 4.267 ± 0.444
0.595LysTrp: 0.595 ± 0.163
2.481LysTyr: 2.481 ± 0.417
0.0LysXaa: 0.0 ± 0.0
Leu
6.152LeuAla: 6.152 ± 0.506
0.992LeuCys: 0.992 ± 0.214
3.622LeuAsp: 3.622 ± 0.386
4.713LeuGlu: 4.713 ± 0.519
2.133LeuPhe: 2.133 ± 0.354
4.713LeuGly: 4.713 ± 0.542
0.992LeuHis: 0.992 ± 0.2
4.317LeuIle: 4.317 ± 0.559
6.4LeuLys: 6.4 ± 0.621
4.515LeuLeu: 4.515 ± 0.386
2.133LeuMet: 2.133 ± 0.325
4.366LeuAsn: 4.366 ± 0.568
3.175LeuPro: 3.175 ± 0.497
2.927LeuGln: 2.927 ± 0.44
4.019LeuArg: 4.019 ± 0.531
5.755LeuSer: 5.755 ± 0.604
5.755LeuThr: 5.755 ± 0.396
4.168LeuVal: 4.168 ± 0.424
0.695LeuTrp: 0.695 ± 0.16
2.133LeuTyr: 2.133 ± 0.337
0.0LeuXaa: 0.0 ± 0.0
Met
2.679MetAla: 2.679 ± 0.394
0.248MetCys: 0.248 ± 0.113
1.141MetAsp: 1.141 ± 0.273
1.538MetGlu: 1.538 ± 0.356
0.992MetPhe: 0.992 ± 0.213
1.538MetGly: 1.538 ± 0.209
0.248MetHis: 0.248 ± 0.105
1.439MetIle: 1.439 ± 0.287
2.084MetLys: 2.084 ± 0.383
1.985MetLeu: 1.985 ± 0.261
0.595MetMet: 0.595 ± 0.177
2.183MetAsn: 2.183 ± 0.289
1.24MetPro: 1.24 ± 0.304
1.637MetGln: 1.637 ± 0.304
1.637MetArg: 1.637 ± 0.296
2.133MetSer: 2.133 ± 0.38
1.985MetThr: 1.985 ± 0.409
1.885MetVal: 1.885 ± 0.285
0.298MetTrp: 0.298 ± 0.125
0.893MetTyr: 0.893 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
4.217AsnAla: 4.217 ± 0.47
0.347AsnCys: 0.347 ± 0.152
2.233AsnAsp: 2.233 ± 0.298
2.63AsnGlu: 2.63 ± 0.339
1.836AsnPhe: 1.836 ± 0.315
5.16AsnGly: 5.16 ± 0.586
1.141AsnHis: 1.141 ± 0.312
3.92AsnIle: 3.92 ± 0.493
2.977AsnLys: 2.977 ± 0.544
3.473AsnLeu: 3.473 ± 0.428
1.34AsnMet: 1.34 ± 0.285
2.332AsnAsn: 2.332 ± 0.407
2.828AsnPro: 2.828 ± 0.399
1.737AsnGln: 1.737 ± 0.286
2.481AsnArg: 2.481 ± 0.352
2.977AsnSer: 2.977 ± 0.353
3.225AsnThr: 3.225 ± 0.429
3.275AsnVal: 3.275 ± 0.345
0.595AsnTrp: 0.595 ± 0.153
1.935AsnTyr: 1.935 ± 0.367
0.0AsnXaa: 0.0 ± 0.0
Pro
2.481ProAla: 2.481 ± 0.368
0.298ProCys: 0.298 ± 0.115
2.58ProAsp: 2.58 ± 0.348
3.027ProGlu: 3.027 ± 0.435
1.588ProPhe: 1.588 ± 0.327
2.183ProGly: 2.183 ± 0.359
0.744ProHis: 0.744 ± 0.211
2.183ProIle: 2.183 ± 0.355
2.233ProLys: 2.233 ± 0.288
2.878ProLeu: 2.878 ± 0.328
0.992ProMet: 0.992 ± 0.268
2.431ProAsn: 2.431 ± 0.367
1.24ProPro: 1.24 ± 0.268
1.24ProGln: 1.24 ± 0.227
1.538ProArg: 1.538 ± 0.249
2.382ProSer: 2.382 ± 0.401
2.183ProThr: 2.183 ± 0.368
3.126ProVal: 3.126 ± 0.436
0.695ProTrp: 0.695 ± 0.191
1.439ProTyr: 1.439 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
3.622GlnAla: 3.622 ± 0.521
0.248GlnCys: 0.248 ± 0.104
1.588GlnAsp: 1.588 ± 0.317
1.935GlnGlu: 1.935 ± 0.384
1.885GlnPhe: 1.885 ± 0.246
2.481GlnGly: 2.481 ± 0.409
0.744GlnHis: 0.744 ± 0.232
2.927GlnIle: 2.927 ± 0.376
2.332GlnLys: 2.332 ± 0.369
3.572GlnLeu: 3.572 ± 0.445
0.893GlnMet: 0.893 ± 0.226
1.836GlnAsn: 1.836 ± 0.309
1.24GlnPro: 1.24 ± 0.264
1.885GlnGln: 1.885 ± 0.287
2.53GlnArg: 2.53 ± 0.43
2.58GlnSer: 2.58 ± 0.465
1.885GlnThr: 1.885 ± 0.361
2.58GlnVal: 2.58 ± 0.345
0.347GlnTrp: 0.347 ± 0.136
1.29GlnTyr: 1.29 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
4.217ArgAla: 4.217 ± 0.557
0.744ArgCys: 0.744 ± 0.171
2.58ArgAsp: 2.58 ± 0.323
2.927ArgGlu: 2.927 ± 0.429
1.885ArgPhe: 1.885 ± 0.349
3.076ArgGly: 3.076 ± 0.375
0.843ArgHis: 0.843 ± 0.2
3.324ArgIle: 3.324 ± 0.347
3.374ArgLys: 3.374 ± 0.54
4.317ArgLeu: 4.317 ± 0.495
1.34ArgMet: 1.34 ± 0.244
2.431ArgAsn: 2.431 ± 0.361
1.687ArgPro: 1.687 ± 0.301
2.382ArgGln: 2.382 ± 0.431
2.084ArgArg: 2.084 ± 0.353
3.175ArgSer: 3.175 ± 0.409
2.382ArgThr: 2.382 ± 0.41
3.672ArgVal: 3.672 ± 0.434
0.595ArgTrp: 0.595 ± 0.158
2.183ArgTyr: 2.183 ± 0.295
0.0ArgXaa: 0.0 ± 0.0
Ser
4.962SerAla: 4.962 ± 0.605
0.397SerCys: 0.397 ± 0.127
3.324SerAsp: 3.324 ± 0.38
3.92SerGlu: 3.92 ± 0.53
3.126SerPhe: 3.126 ± 0.464
6.053SerGly: 6.053 ± 0.534
0.992SerHis: 0.992 ± 0.235
4.912SerIle: 4.912 ± 0.596
3.423SerLys: 3.423 ± 0.447
4.614SerLeu: 4.614 ± 0.585
2.133SerMet: 2.133 ± 0.257
3.374SerAsn: 3.374 ± 0.424
2.282SerPro: 2.282 ± 0.342
1.935SerGln: 1.935 ± 0.35
2.53SerArg: 2.53 ± 0.321
3.82SerSer: 3.82 ± 0.518
4.267SerThr: 4.267 ± 0.641
4.664SerVal: 4.664 ± 0.446
0.893SerTrp: 0.893 ± 0.243
1.737SerTyr: 1.737 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
5.507ThrAla: 5.507 ± 0.74
0.397ThrCys: 0.397 ± 0.171
2.679ThrAsp: 2.679 ± 0.355
2.431ThrGlu: 2.431 ± 0.395
2.729ThrPhe: 2.729 ± 0.371
5.408ThrGly: 5.408 ± 0.585
0.893ThrHis: 0.893 ± 0.229
3.622ThrIle: 3.622 ± 0.489
3.275ThrLys: 3.275 ± 0.384
5.309ThrLeu: 5.309 ± 0.557
1.092ThrMet: 1.092 ± 0.222
2.133ThrAsn: 2.133 ± 0.33
2.729ThrPro: 2.729 ± 0.386
2.133ThrGln: 2.133 ± 0.362
2.729ThrArg: 2.729 ± 0.384
4.664ThrSer: 4.664 ± 0.632
3.473ThrThr: 3.473 ± 0.557
4.664ThrVal: 4.664 ± 0.557
0.695ThrTrp: 0.695 ± 0.163
1.985ThrTyr: 1.985 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
5.805ValAla: 5.805 ± 0.472
0.744ValCys: 0.744 ± 0.199
4.763ValAsp: 4.763 ± 0.629
5.557ValGlu: 5.557 ± 0.641
2.58ValPhe: 2.58 ± 0.356
4.465ValGly: 4.465 ± 0.465
0.794ValHis: 0.794 ± 0.231
4.763ValIle: 4.763 ± 0.568
5.16ValLys: 5.16 ± 0.569
5.011ValLeu: 5.011 ± 0.442
2.332ValMet: 2.332 ± 0.352
3.87ValAsn: 3.87 ± 0.484
3.027ValPro: 3.027 ± 0.415
2.481ValGln: 2.481 ± 0.456
3.523ValArg: 3.523 ± 0.468
4.912ValSer: 4.912 ± 0.689
3.771ValThr: 3.771 ± 0.446
4.068ValVal: 4.068 ± 0.375
0.695ValTrp: 0.695 ± 0.18
2.233ValTyr: 2.233 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
1.29TrpAla: 1.29 ± 0.244
0.149TrpCys: 0.149 ± 0.09
0.695TrpAsp: 0.695 ± 0.227
0.794TrpGlu: 0.794 ± 0.234
0.397TrpPhe: 0.397 ± 0.153
0.496TrpGly: 0.496 ± 0.161
0.149TrpHis: 0.149 ± 0.09
1.042TrpIle: 1.042 ± 0.234
0.645TrpLys: 0.645 ± 0.231
1.389TrpLeu: 1.389 ± 0.227
0.447TrpMet: 0.447 ± 0.118
0.595TrpAsn: 0.595 ± 0.157
0.397TrpPro: 0.397 ± 0.135
0.496TrpGln: 0.496 ± 0.171
0.744TrpArg: 0.744 ± 0.171
0.943TrpSer: 0.943 ± 0.229
0.893TrpThr: 0.893 ± 0.219
0.595TrpVal: 0.595 ± 0.205
0.05TrpTrp: 0.05 ± 0.051
0.744TrpTyr: 0.744 ± 0.239
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.778TyrAla: 2.778 ± 0.33
0.198TyrCys: 0.198 ± 0.121
2.63TyrAsp: 2.63 ± 0.262
1.737TyrGlu: 1.737 ± 0.238
1.687TyrPhe: 1.687 ± 0.296
3.225TyrGly: 3.225 ± 0.444
0.843TyrHis: 0.843 ± 0.209
2.084TyrIle: 2.084 ± 0.352
2.133TyrLys: 2.133 ± 0.438
2.282TyrLeu: 2.282 ± 0.317
0.744TyrMet: 0.744 ± 0.191
1.588TyrAsn: 1.588 ± 0.246
1.34TyrPro: 1.34 ± 0.255
1.538TyrGln: 1.538 ± 0.262
1.985TyrArg: 1.985 ± 0.363
2.034TyrSer: 2.034 ± 0.431
2.53TyrThr: 2.53 ± 0.32
1.588TyrVal: 1.588 ± 0.332
0.695TyrTrp: 0.695 ± 0.195
1.389TyrTyr: 1.389 ± 0.304
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (20156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski