Salmonella phage Jersey
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4X5B5|S4X5B5_9CAUD Uncharacterized protein OS=Salmonella phage Jersey OX=1340534 GN=Jersey_66 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.29 VYY4 pKa = 10.12 IVSGCNSYY12 pKa = 11.03 GDD14 pKa = 4.02 HH15 pKa = 6.33 YY16 pKa = 11.58 VLGVYY21 pKa = 10.21 RR22 pKa = 11.84 SEE24 pKa = 4.42 EE25 pKa = 3.95 EE26 pKa = 3.92 AEE28 pKa = 3.9 AAAEE32 pKa = 4.07 RR33 pKa = 11.84 EE34 pKa = 4.33 RR35 pKa = 11.84 NYY37 pKa = 10.64 HH38 pKa = 6.34 RR39 pKa = 11.84 DD40 pKa = 3.57 SVDD43 pKa = 3.09 VCEE46 pKa = 4.93 WEE48 pKa = 4.22 VQEE51 pKa = 4.76
Molecular weight: 5.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.344
IPC2_protein 4.609
IPC_protein 4.431
Toseland 4.304
ProMoST 4.431
Dawson 4.355
Bjellqvist 4.584
Wikipedia 4.202
Rodwell 4.279
Grimsley 4.215
Solomon 4.355
Lehninger 4.304
Nozaki 4.482
DTASelect 4.546
Thurlkill 4.304
EMBOSS 4.228
Sillero 4.533
Patrickios 0.896
IPC_peptide 4.355
IPC2_peptide 4.533
IPC2.peptide.svr19 4.498
Protein with the highest isoelectric point:
>tr|S4WY42|S4WY42_9CAUD Uncharacterized protein OS=Salmonella phage Jersey OX=1340534 GN=Jersey_65 PE=4 SV=1
MM1 pKa = 7.52 SLATDD6 pKa = 3.7 ILKK9 pKa = 10.24 HH10 pKa = 6.28 AGINLAPPSSAVTKK24 pKa = 10.32 KK25 pKa = 7.19 VTRR28 pKa = 11.84 VKK30 pKa = 10.37 EE31 pKa = 3.69 KK32 pKa = 10.66 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.64 PKK38 pKa = 10.19 PRR40 pKa = 11.84 VKK42 pKa = 10.0 PVNEE46 pKa = 4.14 MPDD49 pKa = 3.55 VYY51 pKa = 10.73 PRR53 pKa = 11.84 IPGVHH58 pKa = 4.6 QPKK61 pKa = 9.16 YY62 pKa = 10.53 CVGKK66 pKa = 10.16 GLWRR70 pKa = 11.84 AHH72 pKa = 6.43 SYY74 pKa = 10.54 DD75 pKa = 3.01 GKK77 pKa = 10.98 KK78 pKa = 10.05 VVNLGEE84 pKa = 4.41 FGSQARR90 pKa = 11.84 AHH92 pKa = 5.3 MAVRR96 pKa = 11.84 KK97 pKa = 7.72 WW98 pKa = 3.05
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.867
IPC_protein 10.262
Toseland 10.891
ProMoST 10.438
Dawson 10.965
Bjellqvist 10.584
Wikipedia 11.111
Rodwell 11.462
Grimsley 10.994
Solomon 11.038
Lehninger 11.023
Nozaki 10.862
DTASelect 10.584
Thurlkill 10.877
EMBOSS 11.272
Sillero 10.891
Patrickios 11.199
IPC_peptide 11.052
IPC2_peptide 9.238
IPC2.peptide.svr19 8.614
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13504
29
852
195.7
21.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.686 ± 0.574
1.118 ± 0.151
5.88 ± 0.262
6.687 ± 0.376
3.799 ± 0.15
7.62 ± 0.275
1.748 ± 0.165
5.11 ± 0.205
6.124 ± 0.285
7.442 ± 0.257
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.168
4.354 ± 0.219
3.947 ± 0.213
3.68 ± 0.285
5.798 ± 0.253
5.961 ± 0.239
6.272 ± 0.324
7.376 ± 0.323
1.414 ± 0.146
3.429 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here