Synechococcus phage S-CAM7
Average proteome isoelectric point is 5.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 266 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8KTR1|A0A1D8KTR1_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM7 OX=1883368 GN=C490910_085 PE=4 SV=1
MM1 pKa = 7.1 NTLKK5 pKa = 10.79 DD6 pKa = 3.5 LQARR10 pKa = 11.84 VAALIEE16 pKa = 4.12 QQGEE20 pKa = 4.26 DD21 pKa = 4.11 SVCAAWIYY29 pKa = 10.02 TGEE32 pKa = 3.99 DD33 pKa = 3.34 VIRR36 pKa = 11.84 YY37 pKa = 9.27 DD38 pKa = 4.2 EE39 pKa = 4.42 NCDD42 pKa = 4.26 AIRR45 pKa = 11.84 QPKK48 pKa = 8.75 EE49 pKa = 3.71 LSDD52 pKa = 3.7 KK53 pKa = 10.82 VINGLDD59 pKa = 3.74 DD60 pKa = 3.53 YY61 pKa = 11.65 DD62 pKa = 4.69 YY63 pKa = 10.34 IYY65 pKa = 10.94 QVIDD69 pKa = 3.37 EE70 pKa = 5.88 AITQEE75 pKa = 4.71 LDD77 pKa = 3.13 EE78 pKa = 4.8 CVV80 pKa = 3.07
Molecular weight: 9.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A1D8KUU4|A0A1D8KUU4_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM7 OX=1883368 GN=C490910_231 PE=4 SV=1
MM1 pKa = 7.45 IGYY4 pKa = 9.91 INMLAIRR11 pKa = 11.84 SLNGIAKK18 pKa = 9.3 PCDD21 pKa = 3.31 EE22 pKa = 5.63 IIRR25 pKa = 11.84 TTHH28 pKa = 6.01 SLRR31 pKa = 11.84 KK32 pKa = 9.35 SEE34 pKa = 4.17 KK35 pKa = 8.32 PLLRR39 pKa = 11.84 RR40 pKa = 11.84 ASRR43 pKa = 11.84 HH44 pKa = 4.27 LL45 pKa = 3.63
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.794
IPC_protein 10.847
Toseland 11.067
ProMoST 11.067
Dawson 11.111
Bjellqvist 10.906
Wikipedia 11.403
Rodwell 11.213
Grimsley 11.14
Solomon 11.374
Lehninger 11.316
Nozaki 11.052
DTASelect 10.906
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.023
IPC_peptide 11.389
IPC2_peptide 10.175
IPC2.peptide.svr19 8.716
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
266
0
266
66165
38
4314
248.7
27.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.809 ± 0.273
0.886 ± 0.086
6.6 ± 0.124
6.144 ± 0.246
4.3 ± 0.139
8.464 ± 0.431
1.381 ± 0.117
6.788 ± 0.2
4.968 ± 0.372
7.401 ± 0.152
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.874 ± 0.175
5.754 ± 0.191
4.047 ± 0.151
3.434 ± 0.08
4.047 ± 0.152
7.548 ± 0.214
7.726 ± 0.335
7.022 ± 0.286
0.892 ± 0.085
3.916 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here