Amino acid dipepetide frequency for Macrostomum lignano

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.951AlaAla: 16.951 ± 0.084	2.037AlaCys: 2.037 ± 0.014	5.849AlaAsp: 5.849 ± 0.025	6.624AlaGlu: 6.624 ± 0.031	2.839AlaPhe: 2.839 ± 0.013	6.976AlaGly: 6.976 ± 0.028	1.431AlaHis: 1.431 ± 0.01	3.075AlaIle: 3.075 ± 0.016	4.167AlaLys: 4.167 ± 0.027	7.468AlaLeu: 7.468 ± 0.031	1.889AlaMet: 1.889 ± 0.01	3.149AlaAsn: 3.149 ± 0.013	4.477AlaPro: 4.477 ± 0.026	3.479AlaGln: 3.479 ± 0.018	5.158AlaArg: 5.158 ± 0.029	9.252AlaSer: 9.252 ± 0.034	5.426AlaThr: 5.426 ± 0.031	6.299AlaVal: 6.299 ± 0.021	0.939AlaTrp: 0.939 ± 0.008	1.948AlaTyr: 1.948 ± 0.013	0.0AlaXaa: 0.0 ± 0.0
Cys	1.546CysAla: 1.546 ± 0.01	0.799CysCys: 0.799 ± 0.01	1.131CysAsp: 1.131 ± 0.01	0.995CysGlu: 0.995 ± 0.009	0.811CysPhe: 0.811 ± 0.009	1.613CysGly: 1.613 ± 0.013	0.594CysHis: 0.594 ± 0.006	0.862CysIle: 0.862 ± 0.009	0.829CysLys: 0.829 ± 0.01	2.784CysLeu: 2.784 ± 0.02	0.354CysMet: 0.354 ± 0.004	0.752CysAsn: 0.752 ± 0.008	1.31CysPro: 1.31 ± 0.014	1.16CysGln: 1.16 ± 0.009	1.907CysArg: 1.907 ± 0.012	2.029CysSer: 2.029 ± 0.015	1.002CysThr: 1.002 ± 0.009	1.31CysVal: 1.31 ± 0.011	0.284CysTrp: 0.284 ± 0.004	0.605CysTyr: 0.605 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	5.062AspAla: 5.062 ± 0.024	1.495AspCys: 1.495 ± 0.012	3.668AspAsp: 3.668 ± 0.021	4.021AspGlu: 4.021 ± 0.02	2.188AspPhe: 2.188 ± 0.01	3.885AspGly: 3.885 ± 0.019	0.884AspHis: 0.884 ± 0.008	2.077AspIle: 2.077 ± 0.015	2.227AspLys: 2.227 ± 0.016	4.98AspLeu: 4.98 ± 0.019	0.902AspMet: 0.902 ± 0.009	1.896AspAsn: 1.896 ± 0.011	2.627AspPro: 2.627 ± 0.013	2.144AspGln: 2.144 ± 0.012	3.706AspArg: 3.706 ± 0.019	5.419AspSer: 5.419 ± 0.022	2.334AspThr: 2.334 ± 0.011	2.931AspVal: 2.931 ± 0.028	0.859AspTrp: 0.859 ± 0.008	1.452AspTyr: 1.452 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	6.455GluAla: 6.455 ± 0.032	1.021GluCys: 1.021 ± 0.011	3.411GluAsp: 3.411 ± 0.016	4.528GluGlu: 4.528 ± 0.035	2.276GluPhe: 2.276 ± 0.012	2.51GluGly: 2.51 ± 0.013	1.116GluHis: 1.116 ± 0.008	2.291GluIle: 2.291 ± 0.015	2.813GluLys: 2.813 ± 0.021	6.39GluLeu: 6.39 ± 0.027	1.181GluMet: 1.181 ± 0.01	1.918GluAsn: 1.918 ± 0.012	2.724GluPro: 2.724 ± 0.015	3.025GluGln: 3.025 ± 0.019	3.965GluArg: 3.965 ± 0.024	4.583GluSer: 4.583 ± 0.021	2.896GluThr: 2.896 ± 0.016	3.22GluVal: 3.22 ± 0.015	0.532GluTrp: 0.532 ± 0.005	1.198GluTyr: 1.198 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.851PheAla: 2.851 ± 0.013	0.892PheCys: 0.892 ± 0.009	2.212PheAsp: 2.212 ± 0.01	1.953PheGlu: 1.953 ± 0.012	1.348PhePhe: 1.348 ± 0.01	2.492PheGly: 2.492 ± 0.013	0.823PheHis: 0.823 ± 0.007	1.417PheIle: 1.417 ± 0.01	1.444PheLys: 1.444 ± 0.011	3.43PheLeu: 3.43 ± 0.016	0.633PheMet: 0.633 ± 0.006	1.482PheAsn: 1.482 ± 0.01	1.389PhePro: 1.389 ± 0.009	1.346PheGln: 1.346 ± 0.007	2.157PheArg: 2.157 ± 0.012	2.852PheSer: 2.852 ± 0.014	1.661PheThr: 1.661 ± 0.009	2.467PheVal: 2.467 ± 0.014	0.413PheTrp: 0.413 ± 0.006	1.166PheTyr: 1.166 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	5.708GlyAla: 5.708 ± 0.024	1.566GlyCys: 1.566 ± 0.012	3.568GlyAsp: 3.568 ± 0.018	3.187GlyGlu: 3.187 ± 0.018	2.357GlyPhe: 2.357 ± 0.013	7.784GlyGly: 7.784 ± 0.056	1.556GlyHis: 1.556 ± 0.01	2.289GlyIle: 2.289 ± 0.011	2.528GlyLys: 2.528 ± 0.016	5.717GlyLeu: 5.717 ± 0.02	1.032GlyMet: 1.032 ± 0.008	2.237GlyAsn: 2.237 ± 0.015	2.824GlyPro: 2.824 ± 0.022	2.878GlyGln: 2.878 ± 0.016	4.85GlyArg: 4.85 ± 0.023	6.308GlySer: 6.308 ± 0.025	2.744GlyThr: 2.744 ± 0.02	3.766GlyVal: 3.766 ± 0.014	0.724GlyTrp: 0.724 ± 0.006	1.644GlyTyr: 1.644 ± 0.012	0.0GlyXaa: 0.0 ± 0.0
His	1.369HisAla: 1.369 ± 0.009	0.658HisCys: 0.658 ± 0.007	0.837HisAsp: 0.837 ± 0.006	1.012HisGlu: 1.012 ± 0.008	0.882HisPhe: 0.882 ± 0.008	1.32HisGly: 1.32 ± 0.009	0.985HisHis: 0.985 ± 0.011	0.743HisIle: 0.743 ± 0.01	0.777HisLys: 0.777 ± 0.006	2.574HisLeu: 2.574 ± 0.015	0.389HisMet: 0.389 ± 0.005	0.661HisAsn: 0.661 ± 0.006	1.31HisPro: 1.31 ± 0.011	1.69HisGln: 1.69 ± 0.015	2.022HisArg: 2.022 ± 0.012	1.873HisSer: 1.873 ± 0.011	0.837HisThr: 0.837 ± 0.007	1.143HisVal: 1.143 ± 0.008	0.342HisTrp: 0.342 ± 0.004	0.668HisTyr: 0.668 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	2.986IleAla: 2.986 ± 0.014	0.903IleCys: 0.903 ± 0.008	2.473IleAsp: 2.473 ± 0.018	2.342IleGlu: 2.342 ± 0.014	1.381IlePhe: 1.381 ± 0.009	2.703IleGly: 2.703 ± 0.013	0.697IleHis: 0.697 ± 0.007	1.591IleIle: 1.591 ± 0.011	1.669IleLys: 1.669 ± 0.012	3.197IleLeu: 3.197 ± 0.016	0.69IleMet: 0.69 ± 0.006	1.482IleAsn: 1.482 ± 0.009	1.505IlePro: 1.505 ± 0.014	1.381IleGln: 1.381 ± 0.01	2.671IleArg: 2.671 ± 0.016	2.905IleSer: 2.905 ± 0.013	1.608IleThr: 1.608 ± 0.01	2.747IleVal: 2.747 ± 0.014	0.385IleTrp: 0.385 ± 0.005	1.043IleTyr: 1.043 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.439LysAla: 3.439 ± 0.021	0.853LysCys: 0.853 ± 0.011	2.144LysAsp: 2.144 ± 0.016	2.605LysGlu: 2.605 ± 0.019	1.633LysPhe: 1.633 ± 0.011	1.799LysGly: 1.799 ± 0.022	0.859LysHis: 0.859 ± 0.008	1.697LysIle: 1.697 ± 0.011	2.886LysLys: 2.886 ± 0.03	4.264LysLeu: 4.264 ± 0.022	0.856LysMet: 0.856 ± 0.007	1.431LysAsn: 1.431 ± 0.013	2.419LysPro: 2.419 ± 0.017	2.328LysGln: 2.328 ± 0.015	3.34LysArg: 3.34 ± 0.028	3.499LysSer: 3.499 ± 0.022	2.152LysThr: 2.152 ± 0.016	2.316LysVal: 2.316 ± 0.018	0.436LysTrp: 0.436 ± 0.005	1.085LysTyr: 1.085 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	10.001LeuAla: 10.001 ± 0.043	2.215LeuCys: 2.215 ± 0.011	5.202LeuAsp: 5.202 ± 0.021	5.525LeuGlu: 5.525 ± 0.027	3.246LeuPhe: 3.246 ± 0.015	5.211LeuGly: 5.211 ± 0.017	2.581LeuHis: 2.581 ± 0.013	3.761LeuIle: 3.761 ± 0.016	4.134LeuLys: 4.134 ± 0.02	12.042LeuLeu: 12.042 ± 0.046	2.056LeuMet: 2.056 ± 0.011	3.225LeuAsn: 3.225 ± 0.013	5.778LeuPro: 5.778 ± 0.027	4.99LeuGln: 4.99 ± 0.022	7.455LeuArg: 7.455 ± 0.029	8.097LeuSer: 8.097 ± 0.031	5.371LeuThr: 5.371 ± 0.02	6.038LeuVal: 6.038 ± 0.023	0.986LeuTrp: 0.986 ± 0.007	2.181LeuTyr: 2.181 ± 0.012	0.0LeuXaa: 0.0 ± 0.0
Met	1.738MetAla: 1.738 ± 0.009	0.32MetCys: 0.32 ± 0.004	0.983MetAsp: 0.983 ± 0.007	1.234MetGlu: 1.234 ± 0.01	0.606MetPhe: 0.606 ± 0.005	0.895MetGly: 0.895 ± 0.008	0.432MetHis: 0.432 ± 0.004	0.612MetIle: 0.612 ± 0.007	0.879MetLys: 0.879 ± 0.008	2.01MetLeu: 2.01 ± 0.012	0.45MetMet: 0.45 ± 0.006	0.584MetAsn: 0.584 ± 0.006	1.16MetPro: 1.16 ± 0.01	1.094MetGln: 1.094 ± 0.009	1.422MetArg: 1.422 ± 0.01	1.722MetSer: 1.722 ± 0.01	1.009MetThr: 1.009 ± 0.008	0.95MetVal: 0.95 ± 0.009	0.187MetTrp: 0.187 ± 0.004	0.449MetTyr: 0.449 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.612AsnAla: 2.612 ± 0.015	0.953AsnCys: 0.953 ± 0.01	1.68AsnAsp: 1.68 ± 0.01	1.856AsnGlu: 1.856 ± 0.012	1.445AsnPhe: 1.445 ± 0.01	2.522AsnGly: 2.522 ± 0.015	0.668AsnHis: 0.668 ± 0.006	1.363AsnIle: 1.363 ± 0.009	1.482AsnLys: 1.482 ± 0.012	3.224AsnLeu: 3.224 ± 0.014	0.614AsnMet: 0.614 ± 0.006	1.529AsnAsn: 1.529 ± 0.012	1.782AsnPro: 1.782 ± 0.011	1.558AsnGln: 1.558 ± 0.011	2.445AsnArg: 2.445 ± 0.016	3.343AsnSer: 3.343 ± 0.016	1.384AsnThr: 1.384 ± 0.01	1.806AsnVal: 1.806 ± 0.009	0.498AsnTrp: 0.498 ± 0.005	1.069AsnTyr: 1.069 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	6.046ProAla: 6.046 ± 0.031	0.876ProCys: 0.876 ± 0.01	3.27ProAsp: 3.27 ± 0.019	3.232ProGlu: 3.232 ± 0.017	1.42ProPhe: 1.42 ± 0.01	3.538ProGly: 3.538 ± 0.023	1.116ProHis: 1.116 ± 0.008	1.774ProIle: 1.774 ± 0.015	1.979ProLys: 1.979 ± 0.014	4.659ProLeu: 4.659 ± 0.021	1.05ProMet: 1.05 ± 0.009	1.67ProAsn: 1.67 ± 0.012	6.045ProPro: 6.045 ± 0.047	2.788ProGln: 2.788 ± 0.024	2.882ProArg: 2.882 ± 0.017	4.716ProSer: 4.716 ± 0.028	3.13ProThr: 3.13 ± 0.026	3.602ProVal: 3.602 ± 0.018	0.477ProTrp: 0.477 ± 0.005	1.06ProTyr: 1.06 ± 0.008	0.0ProXaa: 0.0 ± 0.0
Gln	3.942GlnAla: 3.942 ± 0.017	0.884GlnCys: 0.884 ± 0.008	1.719GlnAsp: 1.719 ± 0.01	2.422GlnGlu: 2.422 ± 0.016	1.681GlnPhe: 1.681 ± 0.012	1.785GlnGly: 1.785 ± 0.012	1.632GlnHis: 1.632 ± 0.015	1.557GlnIle: 1.557 ± 0.01	1.956GlnLys: 1.956 ± 0.012	6.545GlnLeu: 6.545 ± 0.027	0.978GlnMet: 0.978 ± 0.01	1.262GlnAsn: 1.262 ± 0.009	3.934GlnPro: 3.934 ± 0.026	8.923GlnGln: 8.923 ± 0.078	3.899GlnArg: 3.899 ± 0.018	3.84GlnSer: 3.84 ± 0.021	2.135GlnThr: 2.135 ± 0.014	2.454GlnVal: 2.454 ± 0.012	0.474GlnTrp: 0.474 ± 0.005	0.976GlnTyr: 0.976 ± 0.008	0.0GlnXaa: 0.0 ± 0.0
Arg	5.139ArgAla: 5.139 ± 0.035	1.8ArgCys: 1.8 ± 0.015	3.485ArgAsp: 3.485 ± 0.019	3.568ArgGlu: 3.568 ± 0.02	2.382ArgPhe: 2.382 ± 0.013	3.693ArgGly: 3.693 ± 0.018	2.019ArgHis: 2.019 ± 0.011	2.672ArgIle: 2.672 ± 0.016	2.85ArgLys: 2.85 ± 0.047	9.062ArgLeu: 9.062 ± 0.036	1.323ArgMet: 1.323 ± 0.009	2.163ArgAsn: 2.163 ± 0.013	3.849ArgPro: 3.849 ± 0.019	4.372ArgGln: 4.372 ± 0.022	7.47ArgArg: 7.47 ± 0.068	5.395ArgSer: 5.395 ± 0.025	2.852ArgThr: 2.852 ± 0.014	4.033ArgVal: 4.033 ± 0.017	0.871ArgTrp: 0.871 ± 0.007	1.707ArgTyr: 1.707 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	9.543SerAla: 9.543 ± 0.034	1.757SerCys: 1.757 ± 0.012	4.885SerAsp: 4.885 ± 0.018	4.614SerGlu: 4.614 ± 0.017	2.59SerPhe: 2.59 ± 0.012	7.15SerGly: 7.15 ± 0.026	1.633SerHis: 1.633 ± 0.012	3.041SerIle: 3.041 ± 0.02	3.263SerLys: 3.263 ± 0.018	8.062SerLeu: 8.062 ± 0.026	1.574SerMet: 1.574 ± 0.011	3.146SerAsn: 3.146 ± 0.015	4.543SerPro: 4.543 ± 0.028	3.686SerGln: 3.686 ± 0.019	5.613SerArg: 5.613 ± 0.03	10.16SerSer: 10.16 ± 0.042	4.768SerThr: 4.768 ± 0.035	5.738SerVal: 5.738 ± 0.021	0.849SerTrp: 0.849 ± 0.007	1.755SerTyr: 1.755 ± 0.014	0.0SerXaa: 0.0 ± 0.0
Thr	5.835ThrAla: 5.835 ± 0.041	1.1ThrCys: 1.1 ± 0.011	2.997ThrAsp: 2.997 ± 0.016	3.062ThrGlu: 3.062 ± 0.018	1.556ThrPhe: 1.556 ± 0.01	3.746ThrGly: 3.746 ± 0.022	0.801ThrHis: 0.801 ± 0.009	1.806ThrIle: 1.806 ± 0.014	1.96ThrLys: 1.96 ± 0.013	4.13ThrLeu: 4.13 ± 0.016	0.918ThrMet: 0.918 ± 0.007	1.614ThrAsn: 1.614 ± 0.012	2.977ThrPro: 2.977 ± 0.028	1.744ThrGln: 1.744 ± 0.011	2.681ThrArg: 2.681 ± 0.012	4.275ThrSer: 4.275 ± 0.025	3.348ThrThr: 3.348 ± 0.104	3.79ThrVal: 3.79 ± 0.017	0.483ThrTrp: 0.483 ± 0.005	1.07ThrTyr: 1.07 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	5.819ValAla: 5.819 ± 0.019	1.621ValCys: 1.621 ± 0.014	3.498ValAsp: 3.498 ± 0.021	3.515ValGlu: 3.515 ± 0.016	2.255ValPhe: 2.255 ± 0.013	3.686ValGly: 3.686 ± 0.016	1.365ValHis: 1.365 ± 0.01	2.278ValIle: 2.278 ± 0.013	2.669ValLys: 2.669 ± 0.019	5.577ValLeu: 5.577 ± 0.018	1.124ValMet: 1.124 ± 0.008	2.247ValAsn: 2.247 ± 0.013	2.951ValPro: 2.951 ± 0.013	2.629ValGln: 2.629 ± 0.013	4.155ValArg: 4.155 ± 0.018	5.168ValSer: 5.168 ± 0.016	3.473ValThr: 3.473 ± 0.017	3.66ValVal: 3.66 ± 0.016	0.737ValTrp: 0.737 ± 0.007	1.626ValTyr: 1.626 ± 0.01	0.0ValXaa: 0.0 ± 0.0
Trp	0.706TrpAla: 0.706 ± 0.006	0.267TrpCys: 0.267 ± 0.004	0.551TrpAsp: 0.551 ± 0.005	0.509TrpGlu: 0.509 ± 0.006	0.399TrpPhe: 0.399 ± 0.005	0.477TrpGly: 0.477 ± 0.005	0.3TrpHis: 0.3 ± 0.004	0.449TrpIle: 0.449 ± 0.005	0.494TrpLys: 0.494 ± 0.007	1.438TrpLeu: 1.438 ± 0.011	0.235TrpMet: 0.235 ± 0.003	0.414TrpAsn: 0.414 ± 0.004	0.616TrpPro: 0.616 ± 0.006	0.604TrpGln: 0.604 ± 0.006	1.018TrpArg: 1.018 ± 0.008	0.93TrpSer: 0.93 ± 0.007	0.624TrpThr: 0.624 ± 0.006	0.491TrpVal: 0.491 ± 0.005	0.183TrpTrp: 0.183 ± 0.005	0.285TrpTyr: 0.285 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.687TyrAla: 1.687 ± 0.011	0.66TyrCys: 0.66 ± 0.006	1.193TyrAsp: 1.193 ± 0.008	1.248TyrGlu: 1.248 ± 0.008	1.135TyrPhe: 1.135 ± 0.009	1.482TyrGly: 1.482 ± 0.012	0.599TyrHis: 0.599 ± 0.006	1.004TyrIle: 1.004 ± 0.008	1.031TyrLys: 1.031 ± 0.008	2.54TyrLeu: 2.54 ± 0.016	0.489TyrMet: 0.489 ± 0.005	0.969TyrAsn: 0.969 ± 0.008	1.109TyrPro: 1.109 ± 0.01	1.182TyrGln: 1.182 ± 0.009	1.891TyrArg: 1.891 ± 0.012	2.004TyrSer: 2.004 ± 0.013	1.136TyrThr: 1.136 ± 0.008	1.296TyrVal: 1.296 ± 0.009	0.332TyrTrp: 0.332 ± 0.004	0.898TyrTyr: 0.898 ± 0.008	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 43466 proteins (23504603 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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