Natranaerovirga pectinivora
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2857 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3MMV8|A0A4R3MMV8_9FIRM Uncharacterized protein OS=Natranaerovirga pectinivora OX=682400 GN=EDC18_102332 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 9.88 KK3 pKa = 10.15 ISIFDD8 pKa = 3.86 PAMCCSTGVCGPSVDD23 pKa = 4.33 SEE25 pKa = 4.41 LLRR28 pKa = 11.84 VSTVINNLDD37 pKa = 3.28 KK38 pKa = 11.23 KK39 pKa = 10.24 GIKK42 pKa = 9.13 VEE44 pKa = 4.19 RR45 pKa = 11.84 NGLSTNPEE53 pKa = 3.4 IFITNKK59 pKa = 8.82 TVNDD63 pKa = 4.17 LLNKK67 pKa = 10.13 EE68 pKa = 4.82 GIDD71 pKa = 3.65 VLPITLVDD79 pKa = 3.8 GEE81 pKa = 4.6 VVKK84 pKa = 10.3 TKK86 pKa = 10.54 EE87 pKa = 3.65 YY88 pKa = 8.6 LTNVEE93 pKa = 4.2 FSKK96 pKa = 10.95 ILDD99 pKa = 3.59 IPVDD103 pKa = 3.58 QLQGQVEE110 pKa = 4.49 EE111 pKa = 4.64 SCCDD115 pKa = 4.41 DD116 pKa = 5.34 EE117 pKa = 5.22 EE118 pKa = 6.1 CCDD121 pKa = 5.14 DD122 pKa = 6.42 DD123 pKa = 5.91 DD124 pKa = 6.06 SCGCGCGCDD133 pKa = 5.85 DD134 pKa = 5.12 EE135 pKa = 7.72 DD136 pKa = 6.09 DD137 pKa = 5.99 DD138 pKa = 5.42 PGCSCGDD145 pKa = 3.66 GCCC148 pKa = 4.44
Molecular weight: 15.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.961
Patrickios 0.629
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A4R3MFC2|A0A4R3MFC2_9FIRM Fumarate hydratase subunit beta OS=Natranaerovirga pectinivora OX=682400 GN=EDC18_1124 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.91 KK9 pKa = 8.5 RR10 pKa = 11.84 QRR12 pKa = 11.84 QKK14 pKa = 9.41 EE15 pKa = 3.76 HH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 SGRR28 pKa = 11.84 KK29 pKa = 7.88 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 SLSAA44 pKa = 3.93
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2857
0
2857
886605
29
2825
310.3
35.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.421 ± 0.05
0.926 ± 0.017
5.471 ± 0.035
7.641 ± 0.054
4.428 ± 0.035
6.377 ± 0.045
1.603 ± 0.017
9.974 ± 0.051
7.871 ± 0.061
9.322 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.579 ± 0.023
6.126 ± 0.044
3.035 ± 0.034
2.83 ± 0.023
3.595 ± 0.024
6.017 ± 0.037
5.201 ± 0.034
6.626 ± 0.035
0.74 ± 0.015
4.215 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here