Amino acid dipepetide frequency for Streptomyces sp. SID161

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.74AlaAla: 21.74 ± 0.171
1.124AlaCys: 1.124 ± 0.022
8.702AlaAsp: 8.702 ± 0.066
8.87AlaGlu: 8.87 ± 0.084
3.552AlaPhe: 3.552 ± 0.046
14.041AlaGly: 14.041 ± 0.104
3.034AlaHis: 3.034 ± 0.04
3.05AlaIle: 3.05 ± 0.049
2.616AlaLys: 2.616 ± 0.045
14.912AlaLeu: 14.912 ± 0.1
2.427AlaMet: 2.427 ± 0.032
1.776AlaAsn: 1.776 ± 0.032
7.176AlaPro: 7.176 ± 0.069
3.813AlaGln: 3.813 ± 0.041
11.015AlaArg: 11.015 ± 0.087
5.629AlaSer: 5.629 ± 0.051
6.938AlaThr: 6.938 ± 0.06
12.835AlaVal: 12.835 ± 0.101
1.867AlaTrp: 1.867 ± 0.032
2.793AlaTyr: 2.793 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.159CysAla: 1.159 ± 0.028
0.089CysCys: 0.089 ± 0.006
0.487CysAsp: 0.487 ± 0.016
0.394CysGlu: 0.394 ± 0.014
0.235CysPhe: 0.235 ± 0.011
0.994CysGly: 0.994 ± 0.022
0.196CysHis: 0.196 ± 0.009
0.154CysIle: 0.154 ± 0.009
0.116CysLys: 0.116 ± 0.006
0.818CysLeu: 0.818 ± 0.02
0.126CysMet: 0.126 ± 0.007
0.125CysAsn: 0.125 ± 0.007
0.535CysPro: 0.535 ± 0.016
0.167CysGln: 0.167 ± 0.008
0.646CysArg: 0.646 ± 0.019
0.413CysSer: 0.413 ± 0.014
0.542CysThr: 0.542 ± 0.016
0.69CysVal: 0.69 ± 0.017
0.125CysTrp: 0.125 ± 0.007
0.143CysTyr: 0.143 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.756AspAla: 7.756 ± 0.06
0.43AspCys: 0.43 ± 0.013
3.624AspAsp: 3.624 ± 0.049
3.684AspGlu: 3.684 ± 0.044
1.653AspPhe: 1.653 ± 0.031
6.445AspGly: 6.445 ± 0.057
1.503AspHis: 1.503 ± 0.028
1.828AspIle: 1.828 ± 0.03
1.148AspLys: 1.148 ± 0.03
6.278AspLeu: 6.278 ± 0.048
0.817AspMet: 0.817 ± 0.019
0.877AspAsn: 0.877 ± 0.021
4.626AspPro: 4.626 ± 0.044
1.514AspGln: 1.514 ± 0.031
5.213AspArg: 5.213 ± 0.058
2.44AspSer: 2.44 ± 0.036
3.402AspThr: 3.402 ± 0.041
4.792AspVal: 4.792 ± 0.048
1.066AspTrp: 1.066 ± 0.023
1.082AspTyr: 1.082 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.266GluAla: 7.266 ± 0.07
0.379GluCys: 0.379 ± 0.012
2.809GluAsp: 2.809 ± 0.037
3.558GluGlu: 3.558 ± 0.053
1.372GluPhe: 1.372 ± 0.024
4.226GluGly: 4.226 ± 0.051
1.563GluHis: 1.563 ± 0.026
2.115GluIle: 2.115 ± 0.039
1.311GluLys: 1.311 ± 0.029
6.684GluLeu: 6.684 ± 0.059
0.851GluMet: 0.851 ± 0.02
0.97GluAsn: 0.97 ± 0.023
3.352GluPro: 3.352 ± 0.043
2.166GluGln: 2.166 ± 0.033
5.581GluArg: 5.581 ± 0.059
2.379GluSer: 2.379 ± 0.037
2.918GluThr: 2.918 ± 0.041
4.338GluVal: 4.338 ± 0.05
0.739GluTrp: 0.739 ± 0.018
1.118GluTyr: 1.118 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.704PheAla: 3.704 ± 0.046
0.288PheCys: 0.288 ± 0.012
1.932PheAsp: 1.932 ± 0.028
1.407PheGlu: 1.407 ± 0.027
0.889PhePhe: 0.889 ± 0.021
3.008PheGly: 3.008 ± 0.042
0.637PheHis: 0.637 ± 0.017
0.643PheIle: 0.643 ± 0.019
0.488PheLys: 0.488 ± 0.015
2.628PheLeu: 2.628 ± 0.036
0.372PheMet: 0.372 ± 0.014
0.51PheAsn: 0.51 ± 0.015
1.396PhePro: 1.396 ± 0.028
0.67PheGln: 0.67 ± 0.017
1.93PheArg: 1.93 ± 0.031
1.43PheSer: 1.43 ± 0.025
2.014PheThr: 2.014 ± 0.033
2.254PheVal: 2.254 ± 0.034
0.409PheTrp: 0.409 ± 0.013
0.557PheTyr: 0.557 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
11.314GlyAla: 11.314 ± 0.086
0.867GlyCys: 0.867 ± 0.02
5.285GlyAsp: 5.285 ± 0.052
5.026GlyGlu: 5.026 ± 0.05
2.923GlyPhe: 2.923 ± 0.038
8.877GlyGly: 8.877 ± 0.088
2.539GlyHis: 2.539 ± 0.037
3.235GlyIle: 3.235 ± 0.042
2.263GlyLys: 2.263 ± 0.039
9.646GlyLeu: 9.646 ± 0.068
1.976GlyMet: 1.976 ± 0.029
1.64GlyAsn: 1.64 ± 0.029
5.423GlyPro: 5.423 ± 0.061
2.663GlyGln: 2.663 ± 0.039
8.371GlyArg: 8.371 ± 0.066
5.284GlySer: 5.284 ± 0.053
6.876GlyThr: 6.876 ± 0.056
7.777GlyVal: 7.777 ± 0.06
1.702GlyTrp: 1.702 ± 0.029
2.35GlyTyr: 2.35 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.891HisAla: 2.891 ± 0.034
0.23HisCys: 0.23 ± 0.01
1.377HisAsp: 1.377 ± 0.027
1.253HisGlu: 1.253 ± 0.022
0.664HisPhe: 0.664 ± 0.017
2.638HisGly: 2.638 ± 0.038
0.751HisHis: 0.751 ± 0.02
0.659HisIle: 0.659 ± 0.017
0.352HisLys: 0.352 ± 0.011
2.492HisLeu: 2.492 ± 0.028
0.33HisMet: 0.33 ± 0.013
0.34HisAsn: 0.34 ± 0.014
1.935HisPro: 1.935 ± 0.036
0.62HisGln: 0.62 ± 0.016
2.247HisArg: 2.247 ± 0.035
1.016HisSer: 1.016 ± 0.021
1.43HisThr: 1.43 ± 0.023
1.839HisVal: 1.839 ± 0.033
0.398HisTrp: 0.398 ± 0.014
0.48HisTyr: 0.48 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.459IleAla: 4.459 ± 0.052
0.27IleCys: 0.27 ± 0.012
2.055IleAsp: 2.055 ± 0.037
1.822IleGlu: 1.822 ± 0.035
0.595IlePhe: 0.595 ± 0.017
3.336IleGly: 3.336 ± 0.044
0.581IleHis: 0.581 ± 0.017
0.77IleIle: 0.77 ± 0.022
0.665IleLys: 0.665 ± 0.02
2.254IleLeu: 2.254 ± 0.036
0.389IleMet: 0.389 ± 0.014
0.583IleAsn: 0.583 ± 0.017
1.585IlePro: 1.585 ± 0.028
0.687IleGln: 0.687 ± 0.018
2.17IleArg: 2.17 ± 0.036
1.497IleSer: 1.497 ± 0.025
2.085IleThr: 2.085 ± 0.029
2.555IleVal: 2.555 ± 0.038
0.334IleTrp: 0.334 ± 0.011
0.494IleTyr: 0.494 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.832LysAla: 2.832 ± 0.04
0.109LysCys: 0.109 ± 0.007
1.294LysAsp: 1.294 ± 0.029
1.086LysGlu: 1.086 ± 0.024
0.426LysPhe: 0.426 ± 0.014
1.748LysGly: 1.748 ± 0.034
0.394LysHis: 0.394 ± 0.014
0.799LysIle: 0.799 ± 0.022
0.814LysLys: 0.814 ± 0.028
1.842LysLeu: 1.842 ± 0.03
0.345LysMet: 0.345 ± 0.014
0.473LysAsn: 0.473 ± 0.016
1.178LysPro: 1.178 ± 0.026
0.64LysGln: 0.64 ± 0.019
1.361LysArg: 1.361 ± 0.028
1.038LysSer: 1.038 ± 0.024
1.192LysThr: 1.192 ± 0.027
1.802LysVal: 1.802 ± 0.034
0.253LysTrp: 0.253 ± 0.011
0.432LysTyr: 0.432 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.595LeuAla: 15.595 ± 0.109
0.89LeuCys: 0.89 ± 0.019
6.793LeuAsp: 6.793 ± 0.064
4.669LeuGlu: 4.669 ± 0.051
2.661LeuPhe: 2.661 ± 0.041
9.358LeuGly: 9.358 ± 0.065
2.46LeuHis: 2.46 ± 0.033
3.017LeuIle: 3.017 ± 0.044
1.944LeuLys: 1.944 ± 0.036
11.721LeuLeu: 11.721 ± 0.108
1.615LeuMet: 1.615 ± 0.027
1.603LeuAsn: 1.603 ± 0.028
6.703LeuPro: 6.703 ± 0.062
2.172LeuGln: 2.172 ± 0.032
9.048LeuArg: 9.048 ± 0.073
5.193LeuSer: 5.193 ± 0.05
7.002LeuThr: 7.002 ± 0.061
8.888LeuVal: 8.888 ± 0.077
1.315LeuTrp: 1.315 ± 0.028
1.905LeuTyr: 1.905 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.246MetAla: 2.246 ± 0.031
0.143MetCys: 0.143 ± 0.008
0.856MetAsp: 0.856 ± 0.023
0.718MetGlu: 0.718 ± 0.02
0.451MetPhe: 0.451 ± 0.016
1.245MetGly: 1.245 ± 0.027
0.35MetHis: 0.35 ± 0.012
0.598MetIle: 0.598 ± 0.019
0.39MetLys: 0.39 ± 0.012
1.574MetLeu: 1.574 ± 0.03
0.281MetMet: 0.281 ± 0.013
0.396MetAsn: 0.396 ± 0.013
1.086MetPro: 1.086 ± 0.024
0.425MetGln: 0.425 ± 0.015
1.376MetArg: 1.376 ± 0.024
1.298MetSer: 1.298 ± 0.026
1.551MetThr: 1.551 ± 0.029
1.227MetVal: 1.227 ± 0.022
0.21MetTrp: 0.21 ± 0.01
0.32MetTyr: 0.32 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.039AsnAla: 2.039 ± 0.03
0.154AsnCys: 0.154 ± 0.008
0.874AsnAsp: 0.874 ± 0.021
0.735AsnGlu: 0.735 ± 0.021
0.441AsnPhe: 0.441 ± 0.014
1.718AsnGly: 1.718 ± 0.029
0.377AsnHis: 0.377 ± 0.014
0.587AsnIle: 0.587 ± 0.019
0.381AsnLys: 0.381 ± 0.015
1.574AsnLeu: 1.574 ± 0.027
0.279AsnMet: 0.279 ± 0.011
0.369AsnAsn: 0.369 ± 0.014
1.24AsnPro: 1.24 ± 0.026
0.497AsnGln: 0.497 ± 0.015
1.172AsnArg: 1.172 ± 0.023
0.852AsnSer: 0.852 ± 0.021
1.03AsnThr: 1.03 ± 0.026
1.305AsnVal: 1.305 ± 0.029
0.258AsnTrp: 0.258 ± 0.01
0.371AsnTyr: 0.371 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
9.271ProAla: 9.271 ± 0.08
0.364ProCys: 0.364 ± 0.014
4.569ProAsp: 4.569 ± 0.05
4.26ProGlu: 4.26 ± 0.051
1.524ProPhe: 1.524 ± 0.029
7.089ProGly: 7.089 ± 0.071
1.466ProHis: 1.466 ± 0.025
1.088ProIle: 1.088 ± 0.023
1.072ProLys: 1.072 ± 0.027
5.472ProLeu: 5.472 ± 0.056
0.983ProMet: 0.983 ± 0.022
0.794ProAsn: 0.794 ± 0.02
3.499ProPro: 3.499 ± 0.059
1.741ProGln: 1.741 ± 0.031
4.14ProArg: 4.14 ± 0.047
2.943ProSer: 2.943 ± 0.042
2.947ProThr: 2.947 ± 0.039
5.773ProVal: 5.773 ± 0.053
0.906ProTrp: 0.906 ± 0.022
1.505ProTyr: 1.505 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.769GlnAla: 3.769 ± 0.051
0.167GlnCys: 0.167 ± 0.009
1.443GlnAsp: 1.443 ± 0.026
1.434GlnGlu: 1.434 ± 0.03
0.662GlnPhe: 0.662 ± 0.016
2.337GlnGly: 2.337 ± 0.036
0.672GlnHis: 0.672 ± 0.019
1.033GlnIle: 1.033 ± 0.02
0.593GlnLys: 0.593 ± 0.02
3.012GlnLeu: 3.012 ± 0.038
0.467GlnMet: 0.467 ± 0.014
0.475GlnAsn: 0.475 ± 0.015
1.653GlnPro: 1.653 ± 0.031
1.205GlnGln: 1.205 ± 0.034
2.37GlnArg: 2.37 ± 0.036
1.207GlnSer: 1.207 ± 0.025
1.355GlnThr: 1.355 ± 0.022
2.348GlnVal: 2.348 ± 0.039
0.439GlnTrp: 0.439 ± 0.015
0.593GlnTyr: 0.593 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
10.399ArgAla: 10.399 ± 0.069
0.639ArgCys: 0.639 ± 0.02
4.426ArgAsp: 4.426 ± 0.043
4.9ArgGlu: 4.9 ± 0.061
2.361ArgPhe: 2.361 ± 0.031
6.0ArgGly: 6.0 ± 0.061
2.324ArgHis: 2.324 ± 0.037
3.185ArgIle: 3.185 ± 0.038
1.563ArgLys: 1.563 ± 0.033
9.554ArgLeu: 9.554 ± 0.076
1.734ArgMet: 1.734 ± 0.027
1.29ArgAsn: 1.29 ± 0.024
5.414ArgPro: 5.414 ± 0.053
2.444ArgGln: 2.444 ± 0.034
8.13ArgArg: 8.13 ± 0.068
4.059ArgSer: 4.059 ± 0.043
5.67ArgThr: 5.67 ± 0.06
6.067ArgVal: 6.067 ± 0.054
1.374ArgTrp: 1.374 ± 0.029
1.861ArgTyr: 1.861 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.764SerAla: 6.764 ± 0.057
0.416SerCys: 0.416 ± 0.015
2.534SerAsp: 2.534 ± 0.034
2.15SerGlu: 2.15 ± 0.033
1.486SerPhe: 1.486 ± 0.027
5.87SerGly: 5.87 ± 0.06
0.977SerHis: 0.977 ± 0.02
1.24SerIle: 1.24 ± 0.019
0.918SerLys: 0.918 ± 0.022
4.753SerLeu: 4.753 ± 0.049
0.972SerMet: 0.972 ± 0.022
0.757SerAsn: 0.757 ± 0.019
3.035SerPro: 3.035 ± 0.041
1.161SerGln: 1.161 ± 0.025
3.646SerArg: 3.646 ± 0.036
2.564SerSer: 2.564 ± 0.048
2.838SerThr: 2.838 ± 0.036
4.284SerVal: 4.284 ± 0.046
0.863SerTrp: 0.863 ± 0.018
1.222SerTyr: 1.222 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.453ThrAla: 9.453 ± 0.074
0.439ThrCys: 0.439 ± 0.014
3.745ThrAsp: 3.745 ± 0.043
3.223ThrGlu: 3.223 ± 0.037
1.608ThrPhe: 1.608 ± 0.031
7.19ThrGly: 7.19 ± 0.065
1.237ThrHis: 1.237 ± 0.025
1.526ThrIle: 1.526 ± 0.03
1.039ThrLys: 1.039 ± 0.026
5.733ThrLeu: 5.733 ± 0.047
0.885ThrMet: 0.885 ± 0.021
0.907ThrAsn: 0.907 ± 0.022
4.166ThrPro: 4.166 ± 0.054
1.291ThrGln: 1.291 ± 0.026
4.047ThrArg: 4.047 ± 0.047
3.047ThrSer: 3.047 ± 0.043
3.804ThrThr: 3.804 ± 0.046
6.225ThrVal: 6.225 ± 0.056
0.89ThrTrp: 0.89 ± 0.019
1.343ThrTyr: 1.343 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
10.727ValAla: 10.727 ± 0.087
0.82ValCys: 0.82 ± 0.016
4.949ValAsp: 4.949 ± 0.051
4.55ValGlu: 4.55 ± 0.047
2.506ValPhe: 2.506 ± 0.04
6.542ValGly: 6.542 ± 0.053
2.094ValHis: 2.094 ± 0.03
2.674ValIle: 2.674 ± 0.036
1.638ValLys: 1.638 ± 0.029
9.761ValLeu: 9.761 ± 0.083
1.375ValMet: 1.375 ± 0.024
1.595ValAsn: 1.595 ± 0.026
5.557ValPro: 5.557 ± 0.054
2.148ValGln: 2.148 ± 0.038
7.681ValArg: 7.681 ± 0.069
4.317ValSer: 4.317 ± 0.045
5.902ValThr: 5.902 ± 0.059
8.146ValVal: 8.146 ± 0.077
1.177ValTrp: 1.177 ± 0.024
1.634ValTyr: 1.634 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.705TrpAla: 1.705 ± 0.032
0.158TrpCys: 0.158 ± 0.009
0.835TrpAsp: 0.835 ± 0.021
0.716TrpGlu: 0.716 ± 0.02
0.509TrpPhe: 0.509 ± 0.017
1.028TrpGly: 1.028 ± 0.023
0.382TrpHis: 0.382 ± 0.014
0.526TrpIle: 0.526 ± 0.016
0.355TrpLys: 0.355 ± 0.014
1.753TrpLeu: 1.753 ± 0.032
0.272TrpMet: 0.272 ± 0.011
0.384TrpAsn: 0.384 ± 0.013
0.772TrpPro: 0.772 ± 0.018
0.607TrpGln: 0.607 ± 0.014
1.381TrpArg: 1.381 ± 0.029
0.919TrpSer: 0.919 ± 0.022
1.044TrpThr: 1.044 ± 0.022
0.972TrpVal: 0.972 ± 0.021
0.355TrpTrp: 0.355 ± 0.013
0.365TrpTyr: 0.365 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.822TyrAla: 2.822 ± 0.034
0.18TyrCys: 0.18 ± 0.009
1.587TyrAsp: 1.587 ± 0.029
1.242TyrGlu: 1.242 ± 0.023
0.647TyrPhe: 0.647 ± 0.016
2.348TyrGly: 2.348 ± 0.035
0.406TyrHis: 0.406 ± 0.015
0.46TyrIle: 0.46 ± 0.015
0.387TyrLys: 0.387 ± 0.017
2.091TyrLeu: 2.091 ± 0.035
0.244TyrMet: 0.244 ± 0.01
0.391TyrAsn: 0.391 ± 0.015
1.074TyrPro: 1.074 ± 0.019
0.603TyrGln: 0.603 ± 0.016
1.87TyrArg: 1.87 ± 0.028
0.923TyrSer: 0.923 ± 0.02
1.259TyrThr: 1.259 ± 0.026
1.678TyrVal: 1.678 ± 0.026
0.356TyrTrp: 0.356 ± 0.014
0.443TyrTyr: 0.443 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7974 proteins (2309692 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski