Mycobacterium phage Llij

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; Mycobacterium virus Llij

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19ZU3|Q19ZU3_9CAUD Uncharacterized protein OS=Mycobacterium phage Llij OX=2681623 GN=74 PE=4 SV=1
MM1 pKa = 7.92SGEE4 pKa = 4.04INPEE8 pKa = 3.43GFTRR12 pKa = 11.84YY13 pKa = 10.28GGDD16 pKa = 3.72CTCGPIYY23 pKa = 10.21TYY25 pKa = 10.79GGHH28 pKa = 7.13AEE30 pKa = 4.48PGQFDD35 pKa = 4.88PFCPDD40 pKa = 2.8HH41 pKa = 6.95GNPEE45 pKa = 4.29YY46 pKa = 10.83VASLEE51 pKa = 4.27EE52 pKa = 4.01AA53 pKa = 3.67

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19ZR8|Q19ZR8_9CAUD HNHc domain-containing protein OS=Mycobacterium phage Llij OX=2681623 GN=99 PE=4 SV=1
MM1 pKa = 7.2TAVCEE6 pKa = 4.13QCGSDD11 pKa = 3.46FVRR14 pKa = 11.84PARR17 pKa = 11.84RR18 pKa = 11.84GRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LTCSDD27 pKa = 3.14DD28 pKa = 3.6CKK30 pKa = 10.33RR31 pKa = 11.84ARR33 pKa = 11.84NSRR36 pKa = 11.84LQLDD40 pKa = 3.16RR41 pKa = 11.84DD42 pKa = 3.14KK43 pKa = 10.95RR44 pKa = 11.84YY45 pKa = 10.28RR46 pKa = 11.84EE47 pKa = 3.71EE48 pKa = 4.6HH49 pKa = 5.43GAWRR53 pKa = 11.84EE54 pKa = 3.73KK55 pKa = 10.66RR56 pKa = 11.84RR57 pKa = 11.84MRR59 pKa = 11.84SYY61 pKa = 11.32PEE63 pKa = 3.73TCGVCGRR70 pKa = 11.84TFDD73 pKa = 4.93AKK75 pKa = 10.68RR76 pKa = 11.84KK77 pKa = 8.62GQALCSVEE85 pKa = 4.0CQFAVRR91 pKa = 11.84NRR93 pKa = 11.84AAHH96 pKa = 5.31EE97 pKa = 4.21ANRR100 pKa = 11.84QSSIQRR106 pKa = 11.84RR107 pKa = 11.84LPVLYY112 pKa = 9.92TGDD115 pKa = 3.4GVVRR119 pKa = 11.84ATHH122 pKa = 5.49RR123 pKa = 11.84TWTSGLCASCGEE135 pKa = 4.15WFVAGVRR142 pKa = 11.84ARR144 pKa = 11.84YY145 pKa = 9.05CSKK148 pKa = 10.51RR149 pKa = 11.84CAEE152 pKa = 4.16RR153 pKa = 11.84ARR155 pKa = 11.84SEE157 pKa = 3.75RR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 11.84ARR162 pKa = 11.84EE163 pKa = 3.75AGVTTSSRR171 pKa = 11.84VRR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84EE176 pKa = 3.36IFEE179 pKa = 4.08RR180 pKa = 11.84DD181 pKa = 3.43GYY183 pKa = 10.65RR184 pKa = 11.84CHH186 pKa = 7.51LCGEE190 pKa = 4.52MTDD193 pKa = 3.86PTQQVPHH200 pKa = 6.77PRR202 pKa = 11.84APTIDD207 pKa = 3.28HH208 pKa = 6.26VLPLSRR214 pKa = 11.84GGSHH218 pKa = 6.57EE219 pKa = 4.08PANCRR224 pKa = 11.84CACFLCNSVKK234 pKa = 10.15SDD236 pKa = 3.2RR237 pKa = 11.84VGWSPRR243 pKa = 11.84RR244 pKa = 11.84MASS247 pKa = 2.91

Molecular weight: 28.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17990

30

1170

179.9

19.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.495 ± 0.41

1.412 ± 0.208

6.609 ± 0.225

5.831 ± 0.301

3.091 ± 0.181

8.844 ± 0.575

2.329 ± 0.197

4.169 ± 0.206

3.402 ± 0.228

7.165 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.116

3.385 ± 0.163

5.964 ± 0.228

3.352 ± 0.185

7.282 ± 0.488

5.825 ± 0.272

6.648 ± 0.24

7.187 ± 0.265

2.357 ± 0.158

2.446 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski