Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k) (Halococcus turkmenicus)
Average proteome isoelectric point is 4.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5113 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2S1U6|D2S1U6_HALTV Uncharacterized protein OS=Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k) OX=543526 GN=Htur_4539 PE=4 SV=1
MM1 pKa = 7.02 KK2 pKa = 9.23 QTRR5 pKa = 11.84 RR6 pKa = 11.84 TYY8 pKa = 11.14 LKK10 pKa = 9.28 GTAASALIGIGALSGLSGSAAAEE33 pKa = 4.31 SNFDD37 pKa = 4.07 LEE39 pKa = 5.47 AGFADD44 pKa = 4.89 TSWLDD49 pKa = 3.49 DD50 pKa = 4.28 DD51 pKa = 5.63 VDD53 pKa = 3.71 VHH55 pKa = 6.73 TITEE59 pKa = 4.21 PTRR62 pKa = 11.84 SAVEE66 pKa = 4.16 SAFSASGARR75 pKa = 11.84 VVVFEE80 pKa = 4.2 TSGTIDD86 pKa = 4.12 LGGNDD91 pKa = 3.76 LAITEE96 pKa = 4.56 DD97 pKa = 4.19 YY98 pKa = 10.75 CWVAGQTAPSPGITFINGQVRR119 pKa = 11.84 ISANNCVVQHH129 pKa = 5.23 IRR131 pKa = 11.84 SRR133 pKa = 11.84 IGPGSDD139 pKa = 4.29 GSIQSNDD146 pKa = 3.3 AFNTADD152 pKa = 3.41 GTQNNVVDD160 pKa = 4.58 HH161 pKa = 6.64 VSASWGTDD169 pKa = 2.56 EE170 pKa = 4.91 CLSVGYY176 pKa = 8.02 DD177 pKa = 3.52 TQDD180 pKa = 3.14 TTVTNCLIYY189 pKa = 10.56 EE190 pKa = 4.46 GLYY193 pKa = 10.7 DD194 pKa = 4.06 PYY196 pKa = 11.51 GNEE199 pKa = 3.63 ADD201 pKa = 3.99 HH202 pKa = 7.08 NYY204 pKa = 10.53 GSLIGDD210 pKa = 4.09 GASNVTLAGNVWGKK224 pKa = 10.05 VRR226 pKa = 11.84 GRR228 pKa = 11.84 APRR231 pKa = 11.84 LKK233 pKa = 10.37 SDD235 pKa = 3.51 TEE237 pKa = 4.35 TVVVNNLLYY246 pKa = 10.88 FFDD249 pKa = 4.26 EE250 pKa = 4.56 SANADD255 pKa = 3.66 DD256 pKa = 4.24 SAVTSFVGNAAICADD271 pKa = 4.48 DD272 pKa = 5.56 DD273 pKa = 4.28 DD274 pKa = 7.06 AILEE278 pKa = 4.33 GSPTAYY284 pKa = 10.31 HH285 pKa = 7.33 ADD287 pKa = 3.71 NIAYY291 pKa = 7.97 DD292 pKa = 3.84 PPMVDD297 pKa = 3.52 EE298 pKa = 4.56 QPIAEE303 pKa = 4.56 PEE305 pKa = 4.5 STSSPPLWPSGLSEE319 pKa = 4.29 MPSGDD324 pKa = 3.73 VEE326 pKa = 4.73 SHH328 pKa = 6.05 NLTNAGARR336 pKa = 11.84 PADD339 pKa = 3.83 RR340 pKa = 11.84 TQNDD344 pKa = 2.79 ARR346 pKa = 11.84 IVQEE350 pKa = 3.64 IADD353 pKa = 4.53 RR354 pKa = 11.84 AGLDD358 pKa = 3.96 YY359 pKa = 11.19 LDD361 pKa = 4.52 SPYY364 pKa = 10.89 DD365 pKa = 3.56 YY366 pKa = 10.53 WVGHH370 pKa = 6.24 HH371 pKa = 7.35 DD372 pKa = 3.85 EE373 pKa = 4.38 VGGYY377 pKa = 9.56 PEE379 pKa = 5.64 LPVNTHH385 pKa = 5.34 SLEE388 pKa = 4.26 VPDD391 pKa = 4.57 SGIRR395 pKa = 11.84 DD396 pKa = 3.82 WLAGWAQAVEE406 pKa = 4.72 EE407 pKa = 5.08 GSSPPDD413 pKa = 3.63 GGSGDD418 pKa = 4.62 DD419 pKa = 4.28 GSSGPIPTGTYY430 pKa = 9.85 EE431 pKa = 3.87 IANVNSGQLLEE442 pKa = 4.4 VADD445 pKa = 4.56 ASTADD450 pKa = 3.76 GANVQQWSATDD461 pKa = 3.35 HH462 pKa = 6.7 ATQQWYY468 pKa = 10.41 VEE470 pKa = 4.03 DD471 pKa = 3.96 TGNGEE476 pKa = 4.36 YY477 pKa = 10.78 VLQNANSGLLLEE489 pKa = 4.74 VADD492 pKa = 5.31 GSTEE496 pKa = 4.05 DD497 pKa = 3.65 GANVQQHH504 pKa = 6.9 ADD506 pKa = 3.74 TGCDD510 pKa = 3.4 CQRR513 pKa = 11.84 WSINDD518 pKa = 3.42 VGNGEE523 pKa = 4.51 YY524 pKa = 10.02 ILEE527 pKa = 4.38 AVHH530 pKa = 6.19 SGKK533 pKa = 10.32 VADD536 pKa = 4.19 VEE538 pKa = 4.62 GASTSDD544 pKa = 3.37 GANVLQWPDD553 pKa = 2.89 TGGANQRR560 pKa = 11.84 WTFDD564 pKa = 3.43 SVV566 pKa = 2.95
Molecular weight: 59.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.961
Patrickios 1.227
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|D2RR95|D2RR95_HALTV Uncharacterized protein OS=Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k) OX=543526 GN=Htur_3629 PE=4 SV=1
MM1 pKa = 7.94 ALRR4 pKa = 11.84 KK5 pKa = 9.81 LLGSKK10 pKa = 8.54 ATRR13 pKa = 11.84 SLTVVSVLSDD23 pKa = 3.23 AKK25 pKa = 10.62 RR26 pKa = 11.84 AFNRR30 pKa = 11.84 GNRR33 pKa = 11.84 TRR35 pKa = 11.84 GLLLVGVAVLAWKK48 pKa = 7.96 WTVLGMAAQGIVKK61 pKa = 10.0 LLRR64 pKa = 11.84 RR65 pKa = 11.84 GGSAASSPAA74 pKa = 3.1
Molecular weight: 7.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.237
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5113
0
5113
1473976
30
1969
288.3
31.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.565 ± 0.052
0.77 ± 0.012
8.632 ± 0.042
9.243 ± 0.052
3.231 ± 0.022
8.321 ± 0.037
1.984 ± 0.016
4.374 ± 0.027
1.713 ± 0.019
8.837 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.753 ± 0.015
2.384 ± 0.019
4.654 ± 0.025
2.428 ± 0.021
6.566 ± 0.039
5.616 ± 0.026
6.465 ± 0.025
8.558 ± 0.039
1.167 ± 0.015
2.739 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here