Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n) (Desulfurococcus kamchatkensis)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Desulfurococcus; Desulfurococcus amylolyticus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8D556|B8D556_DESA1 Aspartate--tRNA ligase OS=Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n) OX=490899 GN=aspS PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.45ALNTVMGLALAGLAILALGTAMAMWYY28 pKa = 7.16EE29 pKa = 4.15TLRR32 pKa = 11.84VNTYY36 pKa = 10.41VEE38 pKa = 4.03TGEE41 pKa = 5.04VKK43 pKa = 10.63VAWADD48 pKa = 3.58WQCSDD53 pKa = 5.16EE54 pKa = 5.39GPDD57 pKa = 3.71PQLPDD62 pKa = 3.39SDD64 pKa = 3.88FHH66 pKa = 7.98NDD68 pKa = 2.96EE69 pKa = 4.66GKK71 pKa = 10.26DD72 pKa = 3.45VAQCIVEE79 pKa = 4.03PEE81 pKa = 4.3VYY83 pKa = 10.14DD84 pKa = 4.08GEE86 pKa = 4.58GNAVKK91 pKa = 10.94VNITLMNAYY100 pKa = 9.17PGYY103 pKa = 10.69NPVITLTVEE112 pKa = 4.28NIGTIPVKK120 pKa = 10.54LLNYY124 pKa = 10.15DD125 pKa = 3.89LTDD128 pKa = 3.45GGYY131 pKa = 10.74DD132 pKa = 3.45NQALSVTITVPEE144 pKa = 4.17DD145 pKa = 3.65TQLHH149 pKa = 6.48PEE151 pKa = 4.29GTHH154 pKa = 7.52DD155 pKa = 3.22ITINIAVLQTASEE168 pKa = 4.33DD169 pKa = 3.49STYY172 pKa = 11.42SFGLEE177 pKa = 3.43FTYY180 pKa = 10.6AQWNEE185 pKa = 4.04VPP187 pKa = 4.45

Molecular weight: 20.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8D5S3|B8D5S3_DESA1 Uncharacterized protein OS=Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n) OX=490899 GN=DKAM_1128 PE=4 SV=1
MM1 pKa = 7.62ARR3 pKa = 11.84FKK5 pKa = 10.87HH6 pKa = 5.09VARR9 pKa = 11.84KK10 pKa = 9.39LRR12 pKa = 11.84LAAALKK18 pKa = 10.32SNRR21 pKa = 11.84AIPIWVSAKK30 pKa = 8.5TRR32 pKa = 11.84LRR34 pKa = 11.84VRR36 pKa = 11.84RR37 pKa = 11.84GFSLRR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 10.85LKK50 pKa = 10.81NII52 pKa = 4.23

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1470

0

1470

400476

37

1334

272.4

30.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.578 ± 0.067

0.738 ± 0.019

4.552 ± 0.042

7.139 ± 0.08

3.262 ± 0.039

7.382 ± 0.05

1.669 ± 0.03

8.511 ± 0.065

5.799 ± 0.067

10.68 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.022

3.462 ± 0.04

4.489 ± 0.038

1.731 ± 0.023

6.192 ± 0.063

6.468 ± 0.064

4.79 ± 0.061

8.574 ± 0.057

1.097 ± 0.025

4.547 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski