Amino acid dipepetide frequency for Mycobacterium phage Turbido

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.488AlaAla: 10.488 ± 1.215
0.549AlaCys: 0.549 ± 0.196
5.244AlaAsp: 5.244 ± 0.544
7.135AlaGlu: 7.135 ± 0.803
4.269AlaPhe: 4.269 ± 0.45
7.683AlaGly: 7.683 ± 0.794
1.707AlaHis: 1.707 ± 0.35
4.269AlaIle: 4.269 ± 0.482
5.0AlaLys: 5.0 ± 0.599
9.696AlaLeu: 9.696 ± 0.75
2.683AlaMet: 2.683 ± 0.362
2.988AlaAsn: 2.988 ± 0.379
5.366AlaPro: 5.366 ± 0.887
4.512AlaGln: 4.512 ± 0.548
6.403AlaArg: 6.403 ± 0.717
4.878AlaSer: 4.878 ± 0.636
5.183AlaThr: 5.183 ± 0.667
7.622AlaVal: 7.622 ± 0.582
1.646AlaTrp: 1.646 ± 0.326
2.439AlaTyr: 2.439 ± 0.398
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.166
0.244CysCys: 0.244 ± 0.145
0.854CysAsp: 0.854 ± 0.239
0.549CysGlu: 0.549 ± 0.168
0.366CysPhe: 0.366 ± 0.17
0.976CysGly: 0.976 ± 0.251
0.244CysHis: 0.244 ± 0.133
0.183CysIle: 0.183 ± 0.123
0.488CysLys: 0.488 ± 0.171
0.854CysLeu: 0.854 ± 0.225
0.305CysMet: 0.305 ± 0.138
0.427CysAsn: 0.427 ± 0.15
0.305CysPro: 0.305 ± 0.146
0.061CysGln: 0.061 ± 0.067
0.671CysArg: 0.671 ± 0.193
0.427CysSer: 0.427 ± 0.157
0.61CysThr: 0.61 ± 0.19
0.549CysVal: 0.549 ± 0.171
0.427CysTrp: 0.427 ± 0.155
0.305CysTyr: 0.305 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
6.708AspAla: 6.708 ± 0.708
0.732AspCys: 0.732 ± 0.224
4.208AspAsp: 4.208 ± 0.484
5.0AspGlu: 5.0 ± 0.739
3.171AspPhe: 3.171 ± 0.577
5.122AspGly: 5.122 ± 0.542
1.159AspHis: 1.159 ± 0.356
3.964AspIle: 3.964 ± 0.586
2.195AspLys: 2.195 ± 0.343
5.305AspLeu: 5.305 ± 0.694
1.829AspMet: 1.829 ± 0.356
1.464AspAsn: 1.464 ± 0.291
4.33AspPro: 4.33 ± 0.59
2.195AspGln: 2.195 ± 0.343
3.232AspArg: 3.232 ± 0.447
2.683AspSer: 2.683 ± 0.416
3.537AspThr: 3.537 ± 0.495
4.391AspVal: 4.391 ± 0.402
1.098AspTrp: 1.098 ± 0.226
2.622AspTyr: 2.622 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
7.439GluAla: 7.439 ± 0.723
0.305GluCys: 0.305 ± 0.175
5.427GluAsp: 5.427 ± 0.687
4.269GluGlu: 4.269 ± 0.563
2.744GluPhe: 2.744 ± 0.395
5.183GluGly: 5.183 ± 0.607
2.134GluHis: 2.134 ± 0.344
3.72GluIle: 3.72 ± 0.438
2.317GluLys: 2.317 ± 0.373
6.891GluLeu: 6.891 ± 0.749
2.073GluMet: 2.073 ± 0.343
2.256GluAsn: 2.256 ± 0.396
3.049GluPro: 3.049 ± 0.46
1.707GluGln: 1.707 ± 0.357
4.695GluArg: 4.695 ± 0.592
3.11GluSer: 3.11 ± 0.45
3.537GluThr: 3.537 ± 0.445
4.33GluVal: 4.33 ± 0.544
1.098GluTrp: 1.098 ± 0.242
2.134GluTyr: 2.134 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
3.415PheAla: 3.415 ± 0.433
0.305PheCys: 0.305 ± 0.133
2.866PheAsp: 2.866 ± 0.505
2.5PheGlu: 2.5 ± 0.357
0.671PhePhe: 0.671 ± 0.23
3.476PheGly: 3.476 ± 0.457
1.037PheHis: 1.037 ± 0.269
1.524PheIle: 1.524 ± 0.26
1.646PheLys: 1.646 ± 0.323
2.561PheLeu: 2.561 ± 0.48
0.671PheMet: 0.671 ± 0.178
1.707PheAsn: 1.707 ± 0.325
1.951PhePro: 1.951 ± 0.37
0.915PheGln: 0.915 ± 0.224
1.951PheArg: 1.951 ± 0.345
1.342PheSer: 1.342 ± 0.307
2.439PheThr: 2.439 ± 0.333
2.439PheVal: 2.439 ± 0.402
0.244PheTrp: 0.244 ± 0.111
0.854PheTyr: 0.854 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
6.586GlyAla: 6.586 ± 0.548
0.671GlyCys: 0.671 ± 0.205
5.427GlyAsp: 5.427 ± 0.604
4.573GlyGlu: 4.573 ± 0.607
3.171GlyPhe: 3.171 ± 0.477
5.671GlyGly: 5.671 ± 0.548
1.403GlyHis: 1.403 ± 0.275
3.293GlyIle: 3.293 ± 0.44
3.964GlyLys: 3.964 ± 0.465
6.464GlyLeu: 6.464 ± 0.705
2.012GlyMet: 2.012 ± 0.315
3.415GlyAsn: 3.415 ± 0.419
3.415GlyPro: 3.415 ± 0.411
3.232GlyGln: 3.232 ± 0.421
3.72GlyArg: 3.72 ± 0.38
3.781GlySer: 3.781 ± 0.462
5.976GlyThr: 5.976 ± 0.759
6.586GlyVal: 6.586 ± 0.647
1.464GlyTrp: 1.464 ± 0.303
2.683GlyTyr: 2.683 ± 0.329
0.0GlyXaa: 0.0 ± 0.0
His
1.89HisAla: 1.89 ± 0.353
0.366HisCys: 0.366 ± 0.143
1.342HisAsp: 1.342 ± 0.286
1.159HisGlu: 1.159 ± 0.227
0.366HisPhe: 0.366 ± 0.14
2.134HisGly: 2.134 ± 0.513
0.427HisHis: 0.427 ± 0.165
1.098HisIle: 1.098 ± 0.252
0.793HisLys: 0.793 ± 0.229
1.342HisLeu: 1.342 ± 0.362
0.305HisMet: 0.305 ± 0.13
0.671HisAsn: 0.671 ± 0.185
1.281HisPro: 1.281 ± 0.272
0.854HisGln: 0.854 ± 0.227
1.464HisArg: 1.464 ± 0.32
0.732HisSer: 0.732 ± 0.218
1.037HisThr: 1.037 ± 0.236
1.464HisVal: 1.464 ± 0.271
0.488HisTrp: 0.488 ± 0.171
0.61HisTyr: 0.61 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.061IleAla: 5.061 ± 0.528
0.793IleCys: 0.793 ± 0.187
3.293IleAsp: 3.293 ± 0.562
5.244IleGlu: 5.244 ± 0.495
1.037IlePhe: 1.037 ± 0.231
4.695IleGly: 4.695 ± 0.546
1.098IleHis: 1.098 ± 0.24
1.89IleIle: 1.89 ± 0.321
2.073IleLys: 2.073 ± 0.363
3.903IleLeu: 3.903 ± 0.428
0.549IleMet: 0.549 ± 0.172
2.317IleAsn: 2.317 ± 0.401
3.415IlePro: 3.415 ± 0.518
1.707IleGln: 1.707 ± 0.339
3.293IleArg: 3.293 ± 0.487
2.988IleSer: 2.988 ± 0.405
2.439IleThr: 2.439 ± 0.4
2.622IleVal: 2.622 ± 0.357
0.366IleTrp: 0.366 ± 0.126
1.098IleTyr: 1.098 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
5.488LysAla: 5.488 ± 0.671
0.366LysCys: 0.366 ± 0.186
2.439LysAsp: 2.439 ± 0.461
2.805LysGlu: 2.805 ± 0.406
0.976LysPhe: 0.976 ± 0.279
3.171LysGly: 3.171 ± 0.473
0.549LysHis: 0.549 ± 0.218
2.866LysIle: 2.866 ± 0.397
3.537LysLys: 3.537 ± 0.569
4.512LysLeu: 4.512 ± 0.604
0.915LysMet: 0.915 ± 0.262
1.524LysAsn: 1.524 ± 0.287
2.317LysPro: 2.317 ± 0.4
1.707LysGln: 1.707 ± 0.3
3.171LysArg: 3.171 ± 0.495
2.378LysSer: 2.378 ± 0.374
2.683LysThr: 2.683 ± 0.395
3.842LysVal: 3.842 ± 0.386
0.854LysTrp: 0.854 ± 0.215
1.342LysTyr: 1.342 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
9.513LeuAla: 9.513 ± 0.709
0.671LeuCys: 0.671 ± 0.221
5.244LeuAsp: 5.244 ± 0.67
5.61LeuGlu: 5.61 ± 0.666
3.049LeuPhe: 3.049 ± 0.352
5.488LeuGly: 5.488 ± 0.777
1.403LeuHis: 1.403 ± 0.354
4.147LeuIle: 4.147 ± 0.518
3.72LeuLys: 3.72 ± 0.594
5.793LeuLeu: 5.793 ± 0.639
2.805LeuMet: 2.805 ± 0.4
2.683LeuAsn: 2.683 ± 0.376
5.0LeuPro: 5.0 ± 0.56
2.317LeuGln: 2.317 ± 0.448
6.342LeuArg: 6.342 ± 0.76
5.671LeuSer: 5.671 ± 0.647
6.525LeuThr: 6.525 ± 0.841
4.451LeuVal: 4.451 ± 0.598
1.707LeuTrp: 1.707 ± 0.303
2.195LeuTyr: 2.195 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
2.744MetAla: 2.744 ± 0.348
0.122MetCys: 0.122 ± 0.09
1.037MetAsp: 1.037 ± 0.243
1.342MetGlu: 1.342 ± 0.272
0.732MetPhe: 0.732 ± 0.204
1.768MetGly: 1.768 ± 0.368
0.61MetHis: 0.61 ± 0.195
1.464MetIle: 1.464 ± 0.274
1.707MetLys: 1.707 ± 0.274
1.829MetLeu: 1.829 ± 0.364
0.488MetMet: 0.488 ± 0.147
0.732MetAsn: 0.732 ± 0.226
1.403MetPro: 1.403 ± 0.326
1.037MetGln: 1.037 ± 0.253
1.707MetArg: 1.707 ± 0.296
2.622MetSer: 2.622 ± 0.352
1.89MetThr: 1.89 ± 0.281
1.22MetVal: 1.22 ± 0.304
0.305MetTrp: 0.305 ± 0.137
0.671MetTyr: 0.671 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
2.988AsnAla: 2.988 ± 0.428
0.488AsnCys: 0.488 ± 0.18
1.89AsnAsp: 1.89 ± 0.314
2.134AsnGlu: 2.134 ± 0.339
0.671AsnPhe: 0.671 ± 0.224
3.354AsnGly: 3.354 ± 0.513
0.976AsnHis: 0.976 ± 0.208
1.646AsnIle: 1.646 ± 0.295
1.524AsnLys: 1.524 ± 0.327
3.415AsnLeu: 3.415 ± 0.421
1.037AsnMet: 1.037 ± 0.243
0.793AsnAsn: 0.793 ± 0.254
2.073AsnPro: 2.073 ± 0.34
0.915AsnGln: 0.915 ± 0.2
1.951AsnArg: 1.951 ± 0.415
1.403AsnSer: 1.403 ± 0.265
1.585AsnThr: 1.585 ± 0.256
2.378AsnVal: 2.378 ± 0.348
1.037AsnTrp: 1.037 ± 0.272
0.976AsnTyr: 0.976 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
5.854ProAla: 5.854 ± 0.818
0.305ProCys: 0.305 ± 0.155
3.842ProAsp: 3.842 ± 0.461
4.695ProGlu: 4.695 ± 0.559
1.951ProPhe: 1.951 ± 0.402
4.939ProGly: 4.939 ± 0.61
0.976ProHis: 0.976 ± 0.257
2.256ProIle: 2.256 ± 0.328
2.073ProLys: 2.073 ± 0.409
4.025ProLeu: 4.025 ± 0.499
1.524ProMet: 1.524 ± 0.287
1.646ProAsn: 1.646 ± 0.297
2.378ProPro: 2.378 ± 0.388
1.646ProGln: 1.646 ± 0.383
3.476ProArg: 3.476 ± 0.517
2.805ProSer: 2.805 ± 0.477
3.659ProThr: 3.659 ± 0.528
3.964ProVal: 3.964 ± 0.545
1.098ProTrp: 1.098 ± 0.326
1.342ProTyr: 1.342 ± 0.278
0.0ProXaa: 0.0 ± 0.0
Gln
4.086GlnAla: 4.086 ± 0.604
0.183GlnCys: 0.183 ± 0.097
1.342GlnAsp: 1.342 ± 0.283
1.768GlnGlu: 1.768 ± 0.311
1.22GlnPhe: 1.22 ± 0.279
2.744GlnGly: 2.744 ± 0.508
0.61GlnHis: 0.61 ± 0.181
2.317GlnIle: 2.317 ± 0.374
1.707GlnLys: 1.707 ± 0.324
3.232GlnLeu: 3.232 ± 0.806
1.098GlnMet: 1.098 ± 0.271
0.61GlnAsn: 0.61 ± 0.187
1.22GlnPro: 1.22 ± 0.346
1.89GlnGln: 1.89 ± 0.405
2.378GlnArg: 2.378 ± 0.456
1.951GlnSer: 1.951 ± 0.326
2.134GlnThr: 2.134 ± 0.404
3.049GlnVal: 3.049 ± 0.358
0.61GlnTrp: 0.61 ± 0.193
0.793GlnTyr: 0.793 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
4.939ArgAla: 4.939 ± 0.682
0.854ArgCys: 0.854 ± 0.238
4.695ArgAsp: 4.695 ± 0.605
5.305ArgGlu: 5.305 ± 0.688
2.317ArgPhe: 2.317 ± 0.394
4.147ArgGly: 4.147 ± 0.518
1.524ArgHis: 1.524 ± 0.303
3.659ArgIle: 3.659 ± 0.428
3.842ArgLys: 3.842 ± 0.542
5.061ArgLeu: 5.061 ± 0.673
1.585ArgMet: 1.585 ± 0.275
2.561ArgAsn: 2.561 ± 0.413
2.927ArgPro: 2.927 ± 0.423
1.89ArgGln: 1.89 ± 0.355
5.671ArgArg: 5.671 ± 0.626
2.805ArgSer: 2.805 ± 0.483
3.171ArgThr: 3.171 ± 0.403
4.573ArgVal: 4.573 ± 0.602
1.281ArgTrp: 1.281 ± 0.257
1.829ArgTyr: 1.829 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
4.512SerAla: 4.512 ± 0.588
0.549SerCys: 0.549 ± 0.184
3.415SerAsp: 3.415 ± 0.52
3.537SerGlu: 3.537 ± 0.408
2.134SerPhe: 2.134 ± 0.384
4.025SerGly: 4.025 ± 0.499
0.915SerHis: 0.915 ± 0.301
2.378SerIle: 2.378 ± 0.33
2.805SerLys: 2.805 ± 0.422
4.573SerLeu: 4.573 ± 0.638
1.22SerMet: 1.22 ± 0.242
1.403SerAsn: 1.403 ± 0.305
3.232SerPro: 3.232 ± 0.517
1.951SerGln: 1.951 ± 0.299
3.354SerArg: 3.354 ± 0.354
2.744SerSer: 2.744 ± 0.413
3.598SerThr: 3.598 ± 0.472
3.11SerVal: 3.11 ± 0.513
1.342SerTrp: 1.342 ± 0.264
1.585SerTyr: 1.585 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
5.732ThrAla: 5.732 ± 0.535
0.61ThrCys: 0.61 ± 0.252
3.842ThrAsp: 3.842 ± 0.566
3.049ThrGlu: 3.049 ± 0.451
2.073ThrPhe: 2.073 ± 0.359
5.122ThrGly: 5.122 ± 0.655
0.793ThrHis: 0.793 ± 0.259
2.622ThrIle: 2.622 ± 0.38
3.293ThrLys: 3.293 ± 0.525
5.061ThrLeu: 5.061 ± 0.649
1.342ThrMet: 1.342 ± 0.283
1.585ThrAsn: 1.585 ± 0.326
5.183ThrPro: 5.183 ± 0.621
2.195ThrGln: 2.195 ± 0.4
3.049ThrArg: 3.049 ± 0.435
3.354ThrSer: 3.354 ± 0.469
4.025ThrThr: 4.025 ± 0.502
5.305ThrVal: 5.305 ± 0.642
1.22ThrTrp: 1.22 ± 0.274
2.073ThrTyr: 2.073 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
7.013ValAla: 7.013 ± 0.706
0.61ValCys: 0.61 ± 0.196
5.305ValAsp: 5.305 ± 0.515
4.878ValGlu: 4.878 ± 0.721
2.439ValPhe: 2.439 ± 0.412
4.451ValGly: 4.451 ± 0.559
1.098ValHis: 1.098 ± 0.295
4.025ValIle: 4.025 ± 0.489
3.476ValLys: 3.476 ± 0.379
5.549ValLeu: 5.549 ± 0.65
1.342ValMet: 1.342 ± 0.245
2.866ValAsn: 2.866 ± 0.5
3.232ValPro: 3.232 ± 0.533
2.134ValGln: 2.134 ± 0.405
4.695ValArg: 4.695 ± 0.635
4.025ValSer: 4.025 ± 0.529
4.512ValThr: 4.512 ± 0.431
4.878ValVal: 4.878 ± 0.468
1.342ValTrp: 1.342 ± 0.303
2.012ValTyr: 2.012 ± 0.452
0.0ValXaa: 0.0 ± 0.0
Trp
2.073TrpAla: 2.073 ± 0.434
0.305TrpCys: 0.305 ± 0.155
1.037TrpAsp: 1.037 ± 0.26
1.037TrpGlu: 1.037 ± 0.229
0.366TrpPhe: 0.366 ± 0.145
1.342TrpGly: 1.342 ± 0.29
0.61TrpHis: 0.61 ± 0.196
0.793TrpIle: 0.793 ± 0.235
0.61TrpLys: 0.61 ± 0.181
1.464TrpLeu: 1.464 ± 0.265
0.671TrpMet: 0.671 ± 0.2
0.732TrpAsn: 0.732 ± 0.21
0.976TrpPro: 0.976 ± 0.27
1.037TrpGln: 1.037 ± 0.284
1.098TrpArg: 1.098 ± 0.239
1.098TrpSer: 1.098 ± 0.287
1.281TrpThr: 1.281 ± 0.269
1.037TrpVal: 1.037 ± 0.2
0.549TrpTrp: 0.549 ± 0.167
0.549TrpTyr: 0.549 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.744TyrAla: 2.744 ± 0.352
0.305TyrCys: 0.305 ± 0.152
2.195TyrAsp: 2.195 ± 0.316
1.829TyrGlu: 1.829 ± 0.329
0.793TyrPhe: 0.793 ± 0.191
2.012TyrGly: 2.012 ± 0.374
0.549TyrHis: 0.549 ± 0.182
1.768TyrIle: 1.768 ± 0.289
0.732TyrLys: 0.732 ± 0.178
2.805TyrLeu: 2.805 ± 0.465
0.915TyrMet: 0.915 ± 0.238
0.854TyrAsn: 0.854 ± 0.22
1.403TyrPro: 1.403 ± 0.292
1.037TyrGln: 1.037 ± 0.238
2.317TyrArg: 2.317 ± 0.446
1.585TyrSer: 1.585 ± 0.364
1.707TyrThr: 1.707 ± 0.283
2.073TyrVal: 2.073 ± 0.38
0.488TyrTrp: 0.488 ± 0.211
0.793TyrTyr: 0.793 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16400 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski