Klamath virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tupavirus; Klamath tupavirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R167|A0A0D3R167_9RHAB Nucleocapsid protein OS=Klamath virus OX=909206 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.2KK3 pKa = 10.0RR4 pKa = 11.84VPSAVLANYY13 pKa = 9.66DD14 pKa = 3.73LSKK17 pKa = 11.04LQDD20 pKa = 3.63SLEE23 pKa = 4.16EE24 pKa = 4.48AEE26 pKa = 4.86GADD29 pKa = 3.75LSEE32 pKa = 4.21MEE34 pKa = 4.26RR35 pKa = 11.84DD36 pKa = 3.53KK37 pKa = 11.78GGSAQSNSSDD47 pKa = 2.96ILNLFDD53 pKa = 5.2PYY55 pKa = 11.45SPGGQSSEE63 pKa = 4.25LLFDD67 pKa = 3.85GLNPIKK73 pKa = 10.31IVAEE77 pKa = 4.13GDD79 pKa = 3.43KK80 pKa = 11.19SEE82 pKa = 4.8SNSDD86 pKa = 5.27DD87 pKa = 3.62EE88 pKa = 5.78DD89 pKa = 5.17ADD91 pKa = 4.2PGDD94 pKa = 4.19RR95 pKa = 11.84GADD98 pKa = 3.23SDD100 pKa = 4.09EE101 pKa = 4.69SSEE104 pKa = 4.34SEE106 pKa = 4.21SDD108 pKa = 3.27HH109 pKa = 7.49GPDD112 pKa = 4.16LSAINHH118 pKa = 6.05PPKK121 pKa = 10.84SRR123 pKa = 11.84DD124 pKa = 3.04NFFNINGPDD133 pKa = 3.31KK134 pKa = 11.06GPYY137 pKa = 8.84SLRR140 pKa = 11.84INPEE144 pKa = 3.94VLQSAPIDD152 pKa = 3.88GQLEE156 pKa = 4.21LLCEE160 pKa = 4.24LLHH163 pKa = 6.51EE164 pKa = 5.15LGTRR168 pKa = 11.84VGFSVKK174 pKa = 10.45ASGRR178 pKa = 11.84YY179 pKa = 8.15FAIDD183 pKa = 3.66KK184 pKa = 10.74IEE186 pKa = 4.24VPSSSIPPGPVQASGNVYY204 pKa = 10.34ARR206 pKa = 11.84YY207 pKa = 9.66KK208 pKa = 10.54EE209 pKa = 3.89RR210 pKa = 11.84LKK212 pKa = 11.3VGFKK216 pKa = 10.48YY217 pKa = 10.04PRR219 pKa = 11.84KK220 pKa = 9.94KK221 pKa = 10.85GGGLLLINEE230 pKa = 4.32EE231 pKa = 4.3SFNVGSLPEE240 pKa = 5.39DD241 pKa = 3.02IFAGQNFKK249 pKa = 10.22TPEE252 pKa = 3.84EE253 pKa = 4.13VLRR256 pKa = 11.84FALARR261 pKa = 11.84KK262 pKa = 9.13GVLAYY267 pKa = 9.87VQTMADD273 pKa = 3.36LL274 pKa = 4.3

Molecular weight: 29.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1B1|A0A0D3R1B1_9RHAB GDP polyribonucleotidyltransferase OS=Klamath virus OX=909206 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84CRR4 pKa = 11.84PNCPICRR11 pKa = 11.84LRR13 pKa = 11.84AGPRR17 pKa = 11.84IWIRR21 pKa = 11.84DD22 pKa = 3.81MVRR25 pKa = 11.84GDD27 pKa = 4.2PPPLNPQPGDD37 pKa = 3.49YY38 pKa = 10.86LFVRR42 pKa = 11.84VPFPPYY48 pKa = 9.64WLRR51 pKa = 11.84LKK53 pKa = 10.65YY54 pKa = 10.52

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

3907

54

2107

488.4

55.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.94 ± 0.716

1.689 ± 0.316

5.426 ± 0.421

5.554 ± 0.479

4.633 ± 0.42

6.168 ± 0.636

2.38 ± 0.443

5.708 ± 0.678

4.965 ± 0.37

11.851 ± 0.934

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.448

3.942 ± 0.359

5.708 ± 0.884

3.481 ± 0.539

6.476 ± 0.658

8.395 ± 0.852

5.298 ± 0.784

5.784 ± 0.694

1.817 ± 0.337

3.43 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski