Jatrophihabitans sp. GAS493
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4295 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286ERM6|A0A286ERM6_9ACTN D-alanine--D-alanine ligase OS=Jatrophihabitans sp. GAS493 OX=1907575 GN=ddl PE=3 SV=1
MM1 pKa = 7.37 HH2 pKa = 7.36 APVATVNGTAIDD14 pKa = 4.06 LTWTSGNDD22 pKa = 3.13 NGGANGSVVTGYY34 pKa = 8.2 TVQFASGDD42 pKa = 3.53 NGTACTTNGAAMGCTVTGLATDD64 pKa = 3.51 TDD66 pKa = 4.12 YY67 pKa = 11.46 TFTVTATNAKK77 pKa = 7.91 GTSPLSPVSNVARR90 pKa = 11.84 IGSTGTGGGGGTCTSTSADD109 pKa = 3.15 GCGSVIVDD117 pKa = 3.43 GSISISLDD125 pKa = 3.25 NADD128 pKa = 4.05 SSFALGGQSGTDD140 pKa = 2.86 SSTPDD145 pKa = 3.02 NTISYY150 pKa = 10.54 VVTTNNAAGYY160 pKa = 8.42 SVSVKK165 pKa = 10.25 SDD167 pKa = 3.35 QPTLTPDD174 pKa = 2.99 SAKK177 pKa = 10.86 NSDD180 pKa = 4.62 KK181 pKa = 10.97 ISFDD185 pKa = 4.25 AITATGTAFVSGTIPNDD202 pKa = 3.51 GTSDD206 pKa = 3.45 GGSAQLATGGTVYY219 pKa = 9.8 TQTARR224 pKa = 11.84 SKK226 pKa = 10.35 ATGDD230 pKa = 3.22 THH232 pKa = 5.35 TTTFGVNIPFVAADD246 pKa = 4.34 AYY248 pKa = 10.66 RR249 pKa = 11.84 GTVTFTASANN259 pKa = 3.55
Molecular weight: 25.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.668
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.973
Rodwell 3.745
Grimsley 3.579
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.444
Thurlkill 3.757
EMBOSS 3.973
Sillero 4.062
Patrickios 1.036
IPC_peptide 3.935
IPC2_peptide 4.024
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A286EIE1|A0A286EIE1_9ACTN EmrB/QacA subfamily drug resistance transporter OS=Jatrophihabitans sp. GAS493 OX=1907575 GN=SAMN05892883_0343 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.17 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.73 KK32 pKa = 9.84
Molecular weight: 4.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4295
0
4295
1461866
29
11849
340.4
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.219 ± 0.06
0.741 ± 0.013
5.795 ± 0.04
5.044 ± 0.055
2.917 ± 0.024
8.851 ± 0.046
2.033 ± 0.026
4.339 ± 0.024
2.069 ± 0.027
10.142 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.72 ± 0.019
2.32 ± 0.034
5.635 ± 0.042
3.178 ± 0.023
6.948 ± 0.068
6.476 ± 0.051
6.524 ± 0.079
8.61 ± 0.034
1.394 ± 0.02
2.046 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here