Stenotrophomonas phage PSH1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Psecadovirus; Stenotrophomonas virus PSH1

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1NI81|B1NI81_9VIRU Zot domain-containing protein OS=Stenotrophomonas phage PSH1 OX=2011087 PE=4 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84TGWLIQLSLLVLLGVAPWSAHH24 pKa = 5.44AQAQQCANNSANCTQPQAYY43 pKa = 8.39AACRR47 pKa = 11.84AEE49 pKa = 4.12LSSYY53 pKa = 10.16IAIRR57 pKa = 11.84KK58 pKa = 9.27DD59 pKa = 3.24FANQNMEE66 pKa = 4.39CVPVGSGGRR75 pKa = 11.84TIQSKK80 pKa = 9.03FQYY83 pKa = 8.42KK84 pKa = 9.59TSANSAWFSGNYY96 pKa = 7.48RR97 pKa = 11.84TYY99 pKa = 9.86TWTKK103 pKa = 8.77GCDD106 pKa = 3.3VEE108 pKa = 4.32PTYY111 pKa = 10.87TGTGPWSSGGSAKK124 pKa = 10.53NGSIGCRR131 pKa = 11.84NGCDD135 pKa = 4.35GMWSTNADD143 pKa = 3.32ATKK146 pKa = 9.02TWTPTGDD153 pKa = 2.96ICPEE157 pKa = 3.97DD158 pKa = 3.49EE159 pKa = 5.71KK160 pKa = 11.5KK161 pKa = 10.81NCDD164 pKa = 3.32HH165 pKa = 6.79MGDD168 pKa = 3.59GYY170 pKa = 11.52YY171 pKa = 9.92WNSLLKK177 pKa = 10.55VCEE180 pKa = 4.19PPEE183 pKa = 4.5GKK185 pKa = 10.35CPGGTKK191 pKa = 10.06PNSLGQCAPEE201 pKa = 4.14PCPDD205 pKa = 4.35GMAQQPDD212 pKa = 4.32GTCKK216 pKa = 10.41KK217 pKa = 10.51KK218 pKa = 10.88EE219 pKa = 4.1NEE221 pKa = 4.21CPAGQVRR228 pKa = 11.84SPDD231 pKa = 3.64GRR233 pKa = 11.84CLPGDD238 pKa = 3.75GQCAKK243 pKa = 10.96GEE245 pKa = 4.1VRR247 pKa = 11.84GPDD250 pKa = 3.59GTCKK254 pKa = 10.1KK255 pKa = 10.75DD256 pKa = 3.2SDD258 pKa = 4.39NDD260 pKa = 4.09GQPDD264 pKa = 3.61EE265 pKa = 4.98PGEE268 pKa = 4.19KK269 pKa = 9.97EE270 pKa = 4.22SFAGGDD276 pKa = 3.95DD277 pKa = 3.52CSMPPSCSGSPILCGQARR295 pKa = 11.84IQWRR299 pKa = 11.84IDD301 pKa = 3.09CNTRR305 pKa = 11.84RR306 pKa = 11.84NRR308 pKa = 11.84NIAGGSCAAMPVCTGEE324 pKa = 3.99KK325 pKa = 9.52CDD327 pKa = 3.47AMEE330 pKa = 3.98YY331 pKa = 10.78AGLLMQWRR339 pKa = 11.84TACAAEE345 pKa = 4.21KK346 pKa = 10.43LASQGNNNGGDD357 pKa = 3.74GAQPEE362 pKa = 4.4WTKK365 pKa = 11.11VGGMSTDD372 pKa = 3.55PGLGASPDD380 pKa = 3.66DD381 pKa = 3.93TKK383 pKa = 11.61VLTVKK388 pKa = 10.75RR389 pKa = 11.84LGVEE393 pKa = 3.89QLDD396 pKa = 3.61QSGFGGGGSCIGFAVSGGSGIGSGFAQAMASPPDD430 pKa = 3.99FFCSYY435 pKa = 10.2IILIRR440 pKa = 11.84AVIILSATVTCAFILTSGGKK460 pKa = 8.72NN461 pKa = 3.0

Molecular weight: 48.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1NI83|B1NI83_9VIRU Uncharacterized protein OS=Stenotrophomonas phage PSH1 OX=2011087 PE=4 SV=1
MM1 pKa = 8.19IEE3 pKa = 4.44FDD5 pKa = 3.21PHH7 pKa = 7.65HH8 pKa = 7.12RR9 pKa = 11.84IDD11 pKa = 5.1LTGPWAGFSFLGDD24 pKa = 3.29RR25 pKa = 11.84LITPEE30 pKa = 3.89GRR32 pKa = 11.84EE33 pKa = 4.23LLPEE37 pKa = 4.29DD38 pKa = 4.58LAWLSLTACQAQEE51 pKa = 3.72WRR53 pKa = 11.84RR54 pKa = 11.84MMEE57 pKa = 4.05AARR60 pKa = 11.84SVPSIDD66 pKa = 3.21SSRR69 pKa = 11.84NVSNRR74 pKa = 11.84NAGIRR79 pKa = 11.84HH80 pKa = 5.87HH81 pKa = 7.06PATVVNLRR89 pKa = 11.84DD90 pKa = 3.5VVSRR94 pKa = 11.84RR95 pKa = 11.84KK96 pKa = 9.6QRR98 pKa = 11.84SAVAIAGPDD107 pKa = 3.64AEE109 pKa = 4.94PPAAGLPIQGPMPRR123 pKa = 11.84QRR125 pKa = 11.84MM126 pKa = 3.56

Molecular weight: 14.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1683

68

461

240.4

26.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.23 ± 0.744

3.268 ± 0.855

5.585 ± 0.447

4.813 ± 0.458

3.209 ± 0.483

9.745 ± 1.095

1.485 ± 0.453

3.922 ± 0.319

4.694 ± 0.603

7.071 ± 0.678

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.328

3.03 ± 0.505

6.239 ± 0.851

4.04 ± 0.461

6.358 ± 0.819

6.001 ± 0.67

5.823 ± 0.733

5.823 ± 0.744

2.139 ± 0.294

3.09 ± 0.649
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski