Pseudomonas phage Littlefix

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Littlefixvirus; Pseudomonas virus Littlefix

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VHS9|A0A2K9VHS9_9CAUD DNA polymerase OS=Pseudomonas phage Littlefix OX=2079289 GN=PsPhLittlefix_gp78 PE=3 SV=1
MM1 pKa = 8.28DD2 pKa = 4.28DD3 pKa = 4.14HH4 pKa = 6.78EE5 pKa = 4.92LAAEE9 pKa = 4.14MVSEE13 pKa = 3.85ILEE16 pKa = 4.3NDD18 pKa = 3.3EE19 pKa = 4.08LLYY22 pKa = 10.52MIGHH26 pKa = 6.45SGGPNPPYY34 pKa = 9.8EE35 pKa = 4.02RR36 pKa = 11.84HH37 pKa = 6.03AEE39 pKa = 4.28IISVTPEE46 pKa = 3.74TLGG49 pKa = 3.53

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VHN8|A0A2K9VHN8_9CAUD Uncharacterized protein OS=Pseudomonas phage Littlefix OX=2079289 GN=PsPhLittlefix_gp48 PE=4 SV=1
MM1 pKa = 7.52QFRR4 pKa = 11.84NKK6 pKa = 9.71LALAIYY12 pKa = 9.3YY13 pKa = 10.04AYY15 pKa = 10.31NKK17 pKa = 10.06NMWEE21 pKa = 4.02HH22 pKa = 5.13VRR24 pKa = 11.84VVRR27 pKa = 11.84EE28 pKa = 3.81PSWFDD33 pKa = 3.01VRR35 pKa = 11.84RR36 pKa = 11.84ISS38 pKa = 4.02

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

23013

38

3369

242.2

26.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.834 ± 0.625

0.834 ± 0.15

6.153 ± 0.188

6.375 ± 0.232

3.411 ± 0.159

6.831 ± 0.277

1.938 ± 0.178

5.132 ± 0.272

6.357 ± 0.31

8.408 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.085 ± 0.181

4.602 ± 0.207

4.298 ± 0.24

4.528 ± 0.228

4.55 ± 0.185

5.666 ± 0.217

6.305 ± 0.314

7.131 ± 0.173

1.204 ± 0.143

3.359 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski