Streptomyces phage Gibson

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AY69|A0A411AY69_9CAUD Uncharacterized protein OS=Streptomyces phage Gibson OX=2500796 GN=81 PE=4 SV=1
MM1 pKa = 7.77SDD3 pKa = 3.49YY4 pKa = 10.17TIPGYY9 pKa = 8.71TVLGDD14 pKa = 3.38VDD16 pKa = 3.83ITQEE20 pKa = 4.05RR21 pKa = 11.84YY22 pKa = 10.42DD23 pKa = 3.77LAGVAMLVIKK33 pKa = 10.7DD34 pKa = 3.48EE35 pKa = 4.25TGEE38 pKa = 4.12LFSLKK43 pKa = 9.81ATGCGCGSNSWCSCAMDD60 pKa = 3.46EE61 pKa = 5.58AMVPEE66 pKa = 4.08NMEE69 pKa = 4.15LVTDD73 pKa = 4.4LEE75 pKa = 4.24ATARR79 pKa = 11.84EE80 pKa = 4.37LYY82 pKa = 10.82DD83 pKa = 4.31DD84 pKa = 4.48VVNSDD89 pKa = 4.03SPDD92 pKa = 3.48VPALRR97 pKa = 11.84AMLIVAIEE105 pKa = 4.19RR106 pKa = 11.84TGYY109 pKa = 8.51TPPALPLEE117 pKa = 4.69GG118 pKa = 4.06

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AY65|A0A411AY65_9CAUD DNA primase OS=Streptomyces phage Gibson OX=2500796 GN=104 PE=4 SV=1
MM1 pKa = 7.63GSLPLPDD8 pKa = 4.86DD9 pKa = 3.89NEE11 pKa = 4.82IVRR14 pKa = 11.84TWNKK18 pKa = 10.3LGNNNSATARR28 pKa = 11.84YY29 pKa = 9.1YY30 pKa = 10.95GVNEE34 pKa = 3.52SSIRR38 pKa = 11.84KK39 pKa = 8.61RR40 pKa = 11.84VARR43 pKa = 11.84ATGRR47 pKa = 11.84KK48 pKa = 6.48PQAYY52 pKa = 8.71KK53 pKa = 10.75RR54 pKa = 11.84LPATGKK60 pKa = 10.15ALHH63 pKa = 6.63EE64 pKa = 4.39EE65 pKa = 4.12LLKK68 pKa = 11.17EE69 pKa = 4.16GTDD72 pKa = 3.39RR73 pKa = 11.84YY74 pKa = 10.96AVGRR78 pKa = 11.84LARR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 8.58GVKK86 pKa = 9.88PQTVRR91 pKa = 11.84AALRR95 pKa = 11.84RR96 pKa = 11.84YY97 pKa = 7.31FTKK100 pKa = 10.9NDD102 pKa = 3.17FF103 pKa = 3.84

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

20586

32

1397

196.1

21.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.488 ± 0.474

1.214 ± 0.137

7.131 ± 0.2

7.155 ± 0.552

2.944 ± 0.164

7.957 ± 0.266

2.035 ± 0.178

4.314 ± 0.179

5.062 ± 0.366

7.612 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.192

3.294 ± 0.16

4.634 ± 0.187

3.663 ± 0.19

6.373 ± 0.364

5.509 ± 0.317

6.237 ± 0.496

6.83 ± 0.268

1.909 ± 0.134

2.895 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski