Vibrio phage Seahorse

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7SEG0|A0A6B7SEG0_9CAUD Uncharacterized protein OS=Vibrio phage Seahorse OX=2662136 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 3.77MSLINLEE9 pKa = 4.07SMIEE13 pKa = 3.71IKK15 pKa = 9.85TYY17 pKa = 9.91EE18 pKa = 4.33DD19 pKa = 3.85CPIDD23 pKa = 3.96EE24 pKa = 5.18EE25 pKa = 4.62LLVWTGDD32 pKa = 3.27SFAIEE37 pKa = 4.56MVSMDD42 pKa = 4.79CDD44 pKa = 3.23TGNYY48 pKa = 8.95YY49 pKa = 10.18PSNGVDD55 pKa = 3.94FVAYY59 pKa = 10.41SEE61 pKa = 5.07LPDD64 pKa = 4.37RR65 pKa = 11.84DD66 pKa = 3.29ATFIKK71 pKa = 10.48FEE73 pKa = 4.42HH74 pKa = 6.23LVEE77 pKa = 4.18

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B7SEW5|A0A6B7SEW5_9CAUD High frequency of lysogenization c family protein OS=Vibrio phage Seahorse OX=2662136 PE=4 SV=1
MM1 pKa = 7.2MEE3 pKa = 4.18SGKK6 pKa = 10.57EE7 pKa = 3.93KK8 pKa = 9.62LWSWFGLSYY17 pKa = 11.29ASFLVLPRR25 pKa = 11.84SFMQEE30 pKa = 4.14MPDD33 pKa = 3.12EE34 pKa = 4.61WQSKK38 pKa = 8.07MADD41 pKa = 3.58LLNEE45 pKa = 3.91YY46 pKa = 10.78NEE48 pKa = 4.43TFKK51 pKa = 11.36SVDD54 pKa = 3.65EE55 pKa = 4.06VDD57 pKa = 3.36KK58 pKa = 11.4VYY60 pKa = 11.31VSARR64 pKa = 11.84KK65 pKa = 9.51NNRR68 pKa = 11.84FTKK71 pKa = 10.26IPDD74 pKa = 3.42WIINYY79 pKa = 8.39KK80 pKa = 10.06RR81 pKa = 11.84PNRR84 pKa = 11.84QKK86 pKa = 9.93IQSMKK91 pKa = 10.54HH92 pKa = 4.4KK93 pKa = 10.63

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11097

67

933

231.2

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.371 ± 0.596

1.334 ± 0.193

6.705 ± 0.263

7.858 ± 0.547

3.496 ± 0.195

6.93 ± 0.405

1.838 ± 0.21

6.155 ± 0.275

7.209 ± 0.501

7.065 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.233

5.299 ± 0.3

3.37 ± 0.263

3.965 ± 0.249

4.803 ± 0.319

6.47 ± 0.344

6.02 ± 0.372

6.29 ± 0.255

1.523 ± 0.192

3.641 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski