Mycobacterium phage Taj

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; Mycobacterium virus Taj

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I7B213|I7B213_9CAUD Uncharacterized protein OS=Mycobacterium phage Taj OX=1204515 GN=96 PE=4 SV=1
MM1 pKa = 7.3TPDD4 pKa = 3.61NVILTHH10 pKa = 7.63DD11 pKa = 4.13GGALQKK17 pKa = 9.76TSLGSWYY24 pKa = 8.69WANDD28 pKa = 3.61DD29 pKa = 4.24QDD31 pKa = 4.62EE32 pKa = 4.39ILPGYY37 pKa = 10.45LIDD40 pKa = 4.48FLPARR45 pKa = 11.84VLYY48 pKa = 10.33IPTDD52 pKa = 3.65SLEE55 pKa = 4.14EE56 pKa = 3.95AA57 pKa = 4.44

Molecular weight:
6.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I7B5V7|I7B5V7_9CAUD Uncharacterized protein OS=Mycobacterium phage Taj OX=1204515 GN=58 PE=4 SV=1
MM1 pKa = 7.49SGRR4 pKa = 11.84FRR6 pKa = 11.84TLEE9 pKa = 3.77QVISEE14 pKa = 4.28WGFDD18 pKa = 3.34TSDD21 pKa = 2.99EE22 pKa = 4.04EE23 pKa = 3.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VRR29 pKa = 11.84WLADD33 pKa = 4.29LIRR36 pKa = 11.84AGKK39 pKa = 9.92VPAHH43 pKa = 6.24KK44 pKa = 10.21FGRR47 pKa = 11.84SWYY50 pKa = 9.72MDD52 pKa = 3.49DD53 pKa = 3.69TDD55 pKa = 3.45IAAALGVLEE64 pKa = 5.5NGFQKK69 pKa = 10.53PVVEE73 pKa = 4.46RR74 pKa = 11.84PVEE77 pKa = 3.9PVRR80 pKa = 11.84RR81 pKa = 11.84GPSAASMRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84LASS94 pKa = 3.18

Molecular weight:
10.8 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

18801

30

1175

170.9

18.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.866 ± 0.498

1.202 ± 0.165

6.867 ± 0.212

5.936 ± 0.301

3.016 ± 0.196

8.861 ± 0.634

2.186 ± 0.158

4.276 ± 0.198

3.213 ± 0.198

7.175 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.124

3.17 ± 0.161

5.883 ± 0.173

3.276 ± 0.182

7.047 ± 0.354

5.962 ± 0.26

6.798 ± 0.228

7.149 ± 0.287

2.314 ± 0.146

2.468 ± 0.147

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski