Mycobacterium phage Taj
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7B213|I7B213_9CAUD Uncharacterized protein OS=Mycobacterium phage Taj OX=1204515 GN=96 PE=4 SV=1
MM1 pKa = 7.3 TPDD4 pKa = 3.61 NVILTHH10 pKa = 7.63 DD11 pKa = 4.13 GGALQKK17 pKa = 9.76 TSLGSWYY24 pKa = 8.69 WANDD28 pKa = 3.61 DD29 pKa = 4.24 QDD31 pKa = 4.62 EE32 pKa = 4.39 ILPGYY37 pKa = 10.45 LIDD40 pKa = 4.48 FLPARR45 pKa = 11.84 VLYY48 pKa = 10.33 IPTDD52 pKa = 3.65 SLEE55 pKa = 4.14 EE56 pKa = 3.95 AA57 pKa = 4.44
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.77
IPC_protein 3.668
Toseland 3.465
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.795
Patrickios 1.875
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|I7B5V7|I7B5V7_9CAUD Uncharacterized protein OS=Mycobacterium phage Taj OX=1204515 GN=58 PE=4 SV=1
MM1 pKa = 7.49 SGRR4 pKa = 11.84 FRR6 pKa = 11.84 TLEE9 pKa = 3.77 QVISEE14 pKa = 4.28 WGFDD18 pKa = 3.34 TSDD21 pKa = 2.99 EE22 pKa = 4.04 EE23 pKa = 3.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 VRR29 pKa = 11.84 WLADD33 pKa = 4.29 LIRR36 pKa = 11.84 AGKK39 pKa = 9.92 VPAHH43 pKa = 6.24 KK44 pKa = 10.21 FGRR47 pKa = 11.84 SWYY50 pKa = 9.72 MDD52 pKa = 3.49 DD53 pKa = 3.69 TDD55 pKa = 3.45 IAAALGVLEE64 pKa = 5.5 NGFQKK69 pKa = 10.53 PVVEE73 pKa = 4.46 RR74 pKa = 11.84 PVEE77 pKa = 3.9 PVRR80 pKa = 11.84 RR81 pKa = 11.84 GPSAASMRR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 LASS94 pKa = 3.18
Molecular weight: 10.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.443
IPC_protein 10.57
Toseland 10.833
ProMoST 10.847
Dawson 10.877
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 10.833
Grimsley 10.906
Solomon 11.155
Lehninger 11.096
Nozaki 10.804
DTASelect 10.687
Thurlkill 10.818
EMBOSS 11.272
Sillero 10.833
Patrickios 10.628
IPC_peptide 11.155
IPC2_peptide 9.78
IPC2.peptide.svr19 8.967
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
110
0
110
18801
30
1175
170.9
18.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.866 ± 0.498
1.202 ± 0.165
6.867 ± 0.212
5.936 ± 0.301
3.016 ± 0.196
8.861 ± 0.634
2.186 ± 0.158
4.276 ± 0.198
3.213 ± 0.198
7.175 ± 0.223
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.124
3.17 ± 0.161
5.883 ± 0.173
3.276 ± 0.182
7.047 ± 0.354
5.962 ± 0.26
6.798 ± 0.228
7.149 ± 0.287
2.314 ± 0.146
2.468 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here