Eubacterium sp. CAG:274

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6PGW5|R6PGW5_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:274 OX=1262888 GN=BN582_00567 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84IEE4 pKa = 4.87PEE6 pKa = 3.97LTYY9 pKa = 10.54QDD11 pKa = 3.6YY12 pKa = 11.09RR13 pKa = 11.84DD14 pKa = 4.05GVEE17 pKa = 3.65HH18 pKa = 6.82CFDD21 pKa = 3.95YY22 pKa = 11.16LGSKK26 pKa = 10.08HH27 pKa = 6.48FEE29 pKa = 4.0TADD32 pKa = 3.93TITDD36 pKa = 3.41WMADD40 pKa = 3.57EE41 pKa = 6.32DD42 pKa = 5.28DD43 pKa = 5.01DD44 pKa = 6.25LLVADD49 pKa = 4.57STSLAII55 pKa = 4.95

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6P996|R6P996_9FIRM tRNA modification GTPase MnmE OS=Eubacterium sp. CAG:274 OX=1262888 GN=mnmE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.33VHH16 pKa = 5.63GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.96VLLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.07GRR39 pKa = 11.84KK40 pKa = 9.04ALTAA44 pKa = 4.17

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2006

0

2006

633728

29

2121

315.9

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.646 ± 0.056

1.457 ± 0.023

5.911 ± 0.045

6.92 ± 0.059

4.17 ± 0.041

6.838 ± 0.056

1.338 ± 0.022

8.455 ± 0.058

8.14 ± 0.051

8.085 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.027

6.16 ± 0.053

2.972 ± 0.028

2.463 ± 0.029

3.276 ± 0.036

6.211 ± 0.049

5.928 ± 0.068

7.386 ± 0.049

0.667 ± 0.015

4.194 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski