Bacillus phage v_B-Bak1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385IJ91|A0A385IJ91_9CAUD Uncharacterized protein OS=Bacillus phage v_B-Bak1 OX=2094735 GN=vBBak1_001 PE=4 SV=1
MM1 pKa = 7.49YY2 pKa = 10.78NMKK5 pKa = 10.4VKK7 pKa = 9.45FTAKK11 pKa = 10.53YY12 pKa = 9.01EE13 pKa = 4.13NKK15 pKa = 10.12EE16 pKa = 4.32DD17 pKa = 3.57GLKK20 pKa = 10.36AYY22 pKa = 9.78EE23 pKa = 5.02FEE25 pKa = 4.44TPDD28 pKa = 4.15YY29 pKa = 10.04IQNDD33 pKa = 2.95IDD35 pKa = 4.83GYY37 pKa = 8.76STDD40 pKa = 4.46IFRR43 pKa = 11.84LDD45 pKa = 3.64CLKK48 pKa = 10.9GEE50 pKa = 4.06WMILEE55 pKa = 4.17QMSDD59 pKa = 3.19APYY62 pKa = 9.64MVSEE66 pKa = 4.59DD67 pKa = 3.62VTHH70 pKa = 7.3LFDD73 pKa = 6.43LDD75 pKa = 5.35DD76 pKa = 5.54IIKK79 pKa = 10.1QMQEE83 pKa = 3.48IEE85 pKa = 4.17NEE87 pKa = 4.19IPCEE91 pKa = 3.69QTEE94 pKa = 4.19YY95 pKa = 10.66NEE97 pKa = 3.65WWEE100 pKa = 4.2EE101 pKa = 3.78EE102 pKa = 4.29GQYY105 pKa = 10.22EE106 pKa = 4.26

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385IJA3|A0A385IJA3_9CAUD Uncharacterized protein OS=Bacillus phage v_B-Bak1 OX=2094735 GN=vBBak1_011 PE=4 SV=1
MM1 pKa = 7.55IGNRR5 pKa = 11.84WVSNPKK11 pKa = 9.95RR12 pKa = 11.84KK13 pKa = 7.4QTRR16 pKa = 11.84ICFFMAHH23 pKa = 5.55TSMFLTGAYY32 pKa = 9.47VPKK35 pKa = 10.14EE36 pKa = 3.95PNRR39 pKa = 11.84VGMILLFCRR48 pKa = 11.84THH50 pKa = 6.34NLSTT54 pKa = 3.72

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

120

0

120

23487

24

2185

195.7

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.02 ± 0.478

0.805 ± 0.11

6.335 ± 0.176

7.634 ± 0.368

4.407 ± 0.178

6.433 ± 0.334

1.614 ± 0.14

7.012 ± 0.225

8.894 ± 0.375

7.706 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.002 ± 0.157

6.148 ± 0.188

2.618 ± 0.181

3.645 ± 0.316

3.823 ± 0.168

5.514 ± 0.286

6.157 ± 0.392

6.659 ± 0.194

1.213 ± 0.085

4.36 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski