Melon chlorotic spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Tenuivirus; Melon tenuivirus

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1Z2Z8|A0A3G1Z2Z8_9VIRU Uncharacterized protein OS=Melon chlorotic spot virus OX=2479459 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 6.49LIEE5 pKa = 4.71VDD7 pKa = 3.56NLYY10 pKa = 11.13DD11 pKa = 4.83LIEE14 pKa = 4.1YY15 pKa = 10.23CKK17 pKa = 10.84AKK19 pKa = 10.44GLNSSCLVPMVLHH32 pKa = 5.62NTGIHH37 pKa = 6.31VDD39 pKa = 3.1INNNDD44 pKa = 2.96FRR46 pKa = 11.84LDD48 pKa = 3.43HH49 pKa = 6.63MDD51 pKa = 3.22WKK53 pKa = 11.16LEE55 pKa = 3.97EE56 pKa = 4.9PDD58 pKa = 3.85PNIEE62 pKa = 3.92INLNLVFEE70 pKa = 5.8DD71 pKa = 3.13IRR73 pKa = 11.84EE74 pKa = 4.35TISMAEE80 pKa = 4.06DD81 pKa = 3.55EE82 pKa = 5.28AWYY85 pKa = 10.29QPDD88 pKa = 4.12EE89 pKa = 4.01ICYY92 pKa = 10.31GIRR95 pKa = 11.84DD96 pKa = 4.11LDD98 pKa = 4.77QLVLWCQKK106 pKa = 9.49EE107 pKa = 4.11RR108 pKa = 11.84TFFPEE113 pKa = 3.85LLSLVFDD120 pKa = 4.22SMPIYY125 pKa = 10.28ISFMEE130 pKa = 4.48PLVFLDD136 pKa = 2.79VDD138 pKa = 4.07EE139 pKa = 5.05PVSIPLYY146 pKa = 10.35HH147 pKa = 6.73SFAEE151 pKa = 4.82MIDD154 pKa = 3.55QVIQDD159 pKa = 3.32IQMEE163 pKa = 4.46IEE165 pKa = 4.15CKK167 pKa = 9.84IYY169 pKa = 10.5HH170 pKa = 7.49DD171 pKa = 5.58DD172 pKa = 3.69YY173 pKa = 11.92DD174 pKa = 4.39SYY176 pKa = 11.72FYY178 pKa = 11.46LLDD181 pKa = 4.11SDD183 pKa = 3.99DD184 pKa = 4.44ARR186 pKa = 11.84WEE188 pKa = 4.42GEE190 pKa = 4.05DD191 pKa = 3.83YY192 pKa = 11.04LYY194 pKa = 11.11DD195 pKa = 3.43

Molecular weight: 23.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1Z1G8|A0A3G1Z1G8_9VIRU Uncharacterized protein OS=Melon chlorotic spot virus OX=2479459 PE=4 SV=1
MM1 pKa = 7.54APVNSLADD9 pKa = 3.34VQGILNQIKK18 pKa = 10.41GVDD21 pKa = 3.9LSDD24 pKa = 3.84SSIEE28 pKa = 3.9QTLSGFVYY36 pKa = 10.46AGFNAVEE43 pKa = 4.12MWKK46 pKa = 9.78MIGSTPGFDD55 pKa = 3.97PKK57 pKa = 10.89DD58 pKa = 3.29IALLICACLQKK69 pKa = 11.02GYY71 pKa = 10.05GLKK74 pKa = 10.54KK75 pKa = 10.35FSTKK79 pKa = 9.41VKK81 pKa = 8.8QTAAKK86 pKa = 8.41NTIDD90 pKa = 5.49AIVLKK95 pKa = 10.59YY96 pKa = 10.49NIKK99 pKa = 8.88PTSKK103 pKa = 10.56DD104 pKa = 3.2SSDD107 pKa = 3.31PTLQRR112 pKa = 11.84IVSTSGIIAFNCYY125 pKa = 7.08QHH127 pKa = 6.45VLSKK131 pKa = 11.03GRR133 pKa = 11.84LNLAVPAASLGLKK146 pKa = 10.31CPDD149 pKa = 3.1VVGCSFINSIIGTVTEE165 pKa = 4.13TSVWEE170 pKa = 3.69PVLIVNEE177 pKa = 3.9YY178 pKa = 10.41LQAQVTMKK186 pKa = 10.38TMSEE190 pKa = 4.15EE191 pKa = 3.82NKK193 pKa = 9.73KK194 pKa = 9.97AQNITTVAEE203 pKa = 4.08AMLKK207 pKa = 10.07NRR209 pKa = 11.84AFSEE213 pKa = 4.4AARR216 pKa = 11.84SSPIVPSGDD225 pKa = 3.09DD226 pKa = 3.68RR227 pKa = 11.84MVLLNYY233 pKa = 9.66FRR235 pKa = 11.84GTNYY239 pKa = 10.05NFSPVSRR246 pKa = 11.84IPEE249 pKa = 4.41EE250 pKa = 3.98ISPGVYY256 pKa = 10.11DD257 pKa = 5.14DD258 pKa = 5.33LKK260 pKa = 10.94LIHH263 pKa = 6.49KK264 pKa = 7.79TLFTPEE270 pKa = 4.1FRR272 pKa = 11.84VQYY275 pKa = 9.86QAIGKK280 pKa = 9.31IGALMKK286 pKa = 10.59SCTQAEE292 pKa = 4.74FPSKK296 pKa = 10.17LTVSYY301 pKa = 11.06FNMAA305 pKa = 3.42

Molecular weight: 33.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

5406

84

2943

415.8

47.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.033 ± 0.45

1.942 ± 0.469

6.03 ± 0.565

6.4 ± 0.409

4.532 ± 0.194

4.107 ± 0.396

1.961 ± 0.251

8.195 ± 0.401

6.641 ± 0.475

10.266 ± 0.495

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.153

5.882 ± 0.295

3.7 ± 0.217

3.108 ± 0.215

4.477 ± 0.415

8.269 ± 0.319

5.771 ± 0.521

5.956 ± 0.33

0.999 ± 0.13

4.624 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski