Amino acid dipepetide frequency for Clostridium sp. CAG:138

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.926AlaAla: 12.926 ± 0.205	1.562AlaCys: 1.562 ± 0.052	5.927AlaAsp: 5.927 ± 0.115	8.505AlaGlu: 8.505 ± 0.166	3.821AlaPhe: 3.821 ± 0.1	6.935AlaGly: 6.935 ± 0.136	1.553AlaHis: 1.553 ± 0.049	4.846AlaIle: 4.846 ± 0.114	5.087AlaLys: 5.087 ± 0.104	9.118AlaLeu: 9.118 ± 0.158	2.624AlaMet: 2.624 ± 0.068	3.123AlaAsn: 3.123 ± 0.079	2.881AlaPro: 2.881 ± 0.08	2.565AlaGln: 2.565 ± 0.068	4.075AlaArg: 4.075 ± 0.103	4.625AlaSer: 4.625 ± 0.096	3.118AlaThr: 3.118 ± 0.088	8.162AlaVal: 8.162 ± 0.133	0.567AlaTrp: 0.567 ± 0.034	2.895AlaTyr: 2.895 ± 0.075	0.004AlaXaa: 0.004 ± 0.003
Cys	1.902CysAla: 1.902 ± 0.059	0.49CysCys: 0.49 ± 0.033	1.054CysAsp: 1.054 ± 0.043	1.16CysGlu: 1.16 ± 0.047	0.85CysPhe: 0.85 ± 0.043	2.361CysGly: 2.361 ± 0.075	0.211CysHis: 0.211 ± 0.02	1.468CysIle: 1.468 ± 0.056	0.777CysLys: 0.777 ± 0.038	1.178CysLeu: 1.178 ± 0.044	0.521CysMet: 0.521 ± 0.027	0.605CysAsn: 0.605 ± 0.034	0.817CysPro: 0.817 ± 0.052	0.222CysGln: 0.222 ± 0.02	1.119CysArg: 1.119 ± 0.046	1.356CysSer: 1.356 ± 0.055	1.067CysThr: 1.067 ± 0.045	1.267CysVal: 1.267 ± 0.041	0.106CysTrp: 0.106 ± 0.015	0.607CysTyr: 0.607 ± 0.038	0.0CysXaa: 0.0 ± 0.0
Asp	5.623AspAla: 5.623 ± 0.116	1.214AspCys: 1.214 ± 0.052	3.123AspAsp: 3.123 ± 0.1	5.15AspGlu: 5.15 ± 0.117	2.806AspPhe: 2.806 ± 0.084	5.134AspGly: 5.134 ± 0.111	0.605AspHis: 0.605 ± 0.035	4.31AspIle: 4.31 ± 0.092	2.817AspLys: 2.817 ± 0.08	3.736AspLeu: 3.736 ± 0.085	1.685AspMet: 1.685 ± 0.056	2.042AspAsn: 2.042 ± 0.057	1.79AspPro: 1.79 ± 0.063	0.596AspGln: 0.596 ± 0.031	2.569AspArg: 2.569 ± 0.068	3.63AspSer: 3.63 ± 0.088	2.713AspThr: 2.713 ± 0.071	3.79AspVal: 3.79 ± 0.091	0.536AspTrp: 0.536 ± 0.037	2.5AspTyr: 2.5 ± 0.075	0.0AspXaa: 0.0 ± 0.0
Glu	5.614GluAla: 5.614 ± 0.125	1.269GluCys: 1.269 ± 0.054	3.088GluAsp: 3.088 ± 0.079	4.244GluGlu: 4.244 ± 0.119	2.881GluPhe: 2.881 ± 0.077	3.703GluGly: 3.703 ± 0.092	1.426GluHis: 1.426 ± 0.065	4.748GluIle: 4.748 ± 0.112	5.017GluLys: 5.017 ± 0.109	7.679GluLeu: 7.679 ± 0.143	2.049GluMet: 2.049 ± 0.067	4.022GluAsn: 4.022 ± 0.093	2.585GluPro: 2.585 ± 0.081	2.682GluGln: 2.682 ± 0.069	4.171GluArg: 4.171 ± 0.093	3.767GluSer: 3.767 ± 0.088	4.042GluThr: 4.042 ± 0.091	3.364GluVal: 3.364 ± 0.076	0.54GluTrp: 0.54 ± 0.032	2.775GluTyr: 2.775 ± 0.081	0.002GluXaa: 0.002 ± 0.002
Phe	4.221PheAla: 4.221 ± 0.106	0.901PheCys: 0.901 ± 0.039	3.056PheAsp: 3.056 ± 0.072	2.897PheGlu: 2.897 ± 0.072	1.945PhePhe: 1.945 ± 0.068	3.668PheGly: 3.668 ± 0.094	0.558PheHis: 0.558 ± 0.029	2.844PheIle: 2.844 ± 0.074	1.796PheLys: 1.796 ± 0.057	3.167PheLeu: 3.167 ± 0.086	1.065PheMet: 1.065 ± 0.047	1.829PheAsn: 1.829 ± 0.06	1.484PhePro: 1.484 ± 0.049	0.713PheGln: 0.713 ± 0.038	2.232PheArg: 2.232 ± 0.057	3.262PheSer: 3.262 ± 0.09	2.459PheThr: 2.459 ± 0.075	3.101PheVal: 3.101 ± 0.082	0.277PheTrp: 0.277 ± 0.022	1.333PheTyr: 1.333 ± 0.048	0.002PheXaa: 0.002 ± 0.002
Gly	6.082GlyAla: 6.082 ± 0.125	1.601GlyCys: 1.601 ± 0.069	3.887GlyAsp: 3.887 ± 0.082	5.072GlyGlu: 5.072 ± 0.112	3.506GlyPhe: 3.506 ± 0.083	5.896GlyGly: 5.896 ± 0.108	1.174GlyHis: 1.174 ± 0.053	6.217GlyIle: 6.217 ± 0.098	4.923GlyLys: 4.923 ± 0.101	5.809GlyLeu: 5.809 ± 0.123	2.45GlyMet: 2.45 ± 0.064	3.041GlyAsn: 3.041 ± 0.079	1.09GlyPro: 1.09 ± 0.047	1.728GlyGln: 1.728 ± 0.059	3.903GlyArg: 3.903 ± 0.091	5.021GlySer: 5.021 ± 0.11	4.387GlyThr: 4.387 ± 0.108	5.092GlyVal: 5.092 ± 0.104	0.642GlyTrp: 0.642 ± 0.035	2.815GlyTyr: 2.815 ± 0.074	0.0GlyXaa: 0.0 ± 0.0
His	1.344HisAla: 1.344 ± 0.048	0.387HisCys: 0.387 ± 0.028	0.935HisAsp: 0.935 ± 0.044	1.15HisGlu: 1.15 ± 0.053	0.698HisPhe: 0.698 ± 0.035	1.632HisGly: 1.632 ± 0.057	0.299HisHis: 0.299 ± 0.028	1.329HisIle: 1.329 ± 0.053	0.811HisLys: 0.811 ± 0.04	1.149HisLeu: 1.149 ± 0.052	0.401HisMet: 0.401 ± 0.027	0.768HisAsn: 0.768 ± 0.04	0.897HisPro: 0.897 ± 0.038	0.306HisGln: 0.306 ± 0.024	0.915HisArg: 0.915 ± 0.036	1.139HisSer: 1.139 ± 0.04	0.944HisThr: 0.944 ± 0.044	1.025HisVal: 1.025 ± 0.048	0.157HisTrp: 0.157 ± 0.015	0.665HisTyr: 0.665 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	7.039IleAla: 7.039 ± 0.121	1.285IleCys: 1.285 ± 0.055	4.23IleAsp: 4.23 ± 0.095	4.717IleGlu: 4.717 ± 0.096	2.423IlePhe: 2.423 ± 0.08	4.965IleGly: 4.965 ± 0.106	1.034IleHis: 1.034 ± 0.045	4.875IleIle: 4.875 ± 0.094	3.267IleLys: 3.267 ± 0.084	5.488IleLeu: 5.488 ± 0.111	1.696IleMet: 1.696 ± 0.061	2.717IleAsn: 2.717 ± 0.074	3.101IlePro: 3.101 ± 0.068	1.358IleGln: 1.358 ± 0.054	3.668IleArg: 3.668 ± 0.083	4.478IleSer: 4.478 ± 0.095	3.676IleThr: 3.676 ± 0.089	4.771IleVal: 4.771 ± 0.103	0.363IleTrp: 0.363 ± 0.027	1.973IleTyr: 1.973 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.569LysAla: 4.569 ± 0.105	0.871LysCys: 0.871 ± 0.043	2.443LysAsp: 2.443 ± 0.067	3.057LysGlu: 3.057 ± 0.079	1.686LysPhe: 1.686 ± 0.059	2.972LysGly: 2.972 ± 0.082	1.183LysHis: 1.183 ± 0.052	3.386LysIle: 3.386 ± 0.082	4.441LysLys: 4.441 ± 0.107	5.893LysLeu: 5.893 ± 0.111	1.475LysMet: 1.475 ± 0.054	2.928LysAsn: 2.928 ± 0.067	2.297LysPro: 2.297 ± 0.069	2.005LysGln: 2.005 ± 0.058	3.386LysArg: 3.386 ± 0.086	3.101LysSer: 3.101 ± 0.077	3.65LysThr: 3.65 ± 0.076	2.884LysVal: 2.884 ± 0.067	0.383LysTrp: 0.383 ± 0.027	2.113LysTyr: 2.113 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	8.12LeuAla: 8.12 ± 0.138	1.993LeuCys: 1.993 ± 0.071	4.735LeuAsp: 4.735 ± 0.104	4.895LeuGlu: 4.895 ± 0.11	4.181LeuPhe: 4.181 ± 0.105	5.889LeuGly: 5.889 ± 0.116	1.688LeuHis: 1.688 ± 0.061	5.679LeuIle: 5.679 ± 0.118	4.578LeuLys: 4.578 ± 0.12	9.103LeuLeu: 9.103 ± 0.195	2.481LeuMet: 2.481 ± 0.068	4.279LeuAsn: 4.279 ± 0.097	4.201LeuPro: 4.201 ± 0.097	2.104LeuGln: 2.104 ± 0.067	5.683LeuArg: 5.683 ± 0.115	6.662LeuSer: 6.662 ± 0.135	5.147LeuThr: 5.147 ± 0.106	5.209LeuVal: 5.209 ± 0.097	0.587LeuTrp: 0.587 ± 0.039	2.848LeuTyr: 2.848 ± 0.073	0.002LeuXaa: 0.002 ± 0.001
Met	1.863MetAla: 1.863 ± 0.064	0.376MetCys: 0.376 ± 0.026	1.433MetAsp: 1.433 ± 0.051	1.366MetGlu: 1.366 ± 0.055	1.061MetPhe: 1.061 ± 0.05	1.712MetGly: 1.712 ± 0.062	0.613MetHis: 0.613 ± 0.032	1.528MetIle: 1.528 ± 0.053	1.892MetLys: 1.892 ± 0.057	3.344MetLeu: 3.344 ± 0.09	0.64MetMet: 0.64 ± 0.033	1.614MetAsn: 1.614 ± 0.059	1.338MetPro: 1.338 ± 0.058	1.136MetGln: 1.136 ± 0.042	1.836MetArg: 1.836 ± 0.062	1.562MetSer: 1.562 ± 0.049	1.535MetThr: 1.535 ± 0.057	1.253MetVal: 1.253 ± 0.051	0.15MetTrp: 0.15 ± 0.017	0.83MetTyr: 0.83 ± 0.038	0.0MetXaa: 0.0 ± 0.0
Asn	4.551AsnAla: 4.551 ± 0.1	0.842AsnCys: 0.842 ± 0.045	2.354AsnAsp: 2.354 ± 0.072	2.875AsnGlu: 2.875 ± 0.07	1.486AsnPhe: 1.486 ± 0.055	4.345AsnGly: 4.345 ± 0.097	0.603AsnHis: 0.603 ± 0.033	3.052AsnIle: 3.052 ± 0.072	2.024AsnLys: 2.024 ± 0.066	3.231AsnLeu: 3.231 ± 0.076	1.211AsnMet: 1.211 ± 0.049	1.816AsnAsn: 1.816 ± 0.069	2.069AsnPro: 2.069 ± 0.051	0.766AsnGln: 0.766 ± 0.036	2.339AsnArg: 2.339 ± 0.068	2.906AsnSer: 2.906 ± 0.075	2.385AsnThr: 2.385 ± 0.07	2.835AsnVal: 2.835 ± 0.073	0.366AsnTrp: 0.366 ± 0.03	1.437AsnTyr: 1.437 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	3.723ProAla: 3.723 ± 0.091	0.702ProCys: 0.702 ± 0.038	2.39ProAsp: 2.39 ± 0.071	3.444ProGlu: 3.444 ± 0.088	1.679ProPhe: 1.679 ± 0.056	2.476ProGly: 2.476 ± 0.068	0.667ProHis: 0.667 ± 0.039	2.024ProIle: 2.024 ± 0.058	1.772ProLys: 1.772 ± 0.061	3.079ProLeu: 3.079 ± 0.082	0.895ProMet: 0.895 ± 0.045	1.69ProAsn: 1.69 ± 0.055	1.112ProPro: 1.112 ± 0.055	1.22ProGln: 1.22 ± 0.049	1.506ProArg: 1.506 ± 0.06	2.057ProSer: 2.057 ± 0.066	2.075ProThr: 2.075 ± 0.08	3.13ProVal: 3.13 ± 0.081	0.303ProTrp: 0.303 ± 0.023	1.322ProTyr: 1.322 ± 0.052	0.004ProXaa: 0.004 ± 0.002
Gln	1.903GlnAla: 1.903 ± 0.062	0.432GlnCys: 0.432 ± 0.029	1.083GlnAsp: 1.083 ± 0.047	1.254GlnGlu: 1.254 ± 0.053	0.954GlnPhe: 0.954 ± 0.044	1.471GlnGly: 1.471 ± 0.054	0.465GlnHis: 0.465 ± 0.028	1.668GlnIle: 1.668 ± 0.054	1.885GlnLys: 1.885 ± 0.058	2.647GlnLeu: 2.647 ± 0.073	0.777GlnMet: 0.777 ± 0.042	1.375GlnAsn: 1.375 ± 0.048	1.023GlnPro: 1.023 ± 0.053	0.985GlnGln: 0.985 ± 0.046	1.559GlnArg: 1.559 ± 0.053	1.677GlnSer: 1.677 ± 0.052	1.49GlnThr: 1.49 ± 0.05	1.356GlnVal: 1.356 ± 0.047	0.228GlnTrp: 0.228 ± 0.022	0.941GlnTyr: 0.941 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	4.191ArgAla: 4.191 ± 0.081	0.952ArgCys: 0.952 ± 0.045	2.686ArgAsp: 2.686 ± 0.068	4.197ArgGlu: 4.197 ± 0.101	2.54ArgPhe: 2.54 ± 0.072	3.455ArgGly: 3.455 ± 0.076	0.921ArgHis: 0.921 ± 0.039	4.547ArgIle: 4.547 ± 0.089	3.236ArgLys: 3.236 ± 0.088	5.099ArgLeu: 5.099 ± 0.096	1.61ArgMet: 1.61 ± 0.054	2.419ArgAsn: 2.419 ± 0.063	1.677ArgPro: 1.677 ± 0.06	1.548ArgGln: 1.548 ± 0.059	3.772ArgArg: 3.772 ± 0.101	3.294ArgSer: 3.294 ± 0.098	2.95ArgThr: 2.95 ± 0.079	3.429ArgVal: 3.429 ± 0.073	0.361ArgTrp: 0.361 ± 0.024	1.799ArgTyr: 1.799 ± 0.065	0.002ArgXaa: 0.002 ± 0.002
Ser	6.671SerAla: 6.671 ± 0.123	1.119SerCys: 1.119 ± 0.046	4.007SerAsp: 4.007 ± 0.095	4.582SerGlu: 4.582 ± 0.107	2.786SerPhe: 2.786 ± 0.072	5.816SerGly: 5.816 ± 0.113	0.952SerHis: 0.952 ± 0.04	3.965SerIle: 3.965 ± 0.088	2.948SerLys: 2.948 ± 0.072	5.068SerLeu: 5.068 ± 0.104	1.541SerMet: 1.541 ± 0.054	2.323SerAsn: 2.323 ± 0.081	2.12SerPro: 2.12 ± 0.075	1.249SerGln: 1.249 ± 0.052	3.183SerArg: 3.183 ± 0.089	3.646SerSer: 3.646 ± 0.092	2.959SerThr: 2.959 ± 0.075	5.264SerVal: 5.264 ± 0.093	0.399SerTrp: 0.399 ± 0.03	1.925SerTyr: 1.925 ± 0.055	0.002SerXaa: 0.002 ± 0.002
Thr	5.976ThrAla: 5.976 ± 0.114	0.768ThrCys: 0.768 ± 0.039	3.36ThrAsp: 3.36 ± 0.074	4.244ThrGlu: 4.244 ± 0.091	2.06ThrPhe: 2.06 ± 0.063	4.306ThrGly: 4.306 ± 0.096	0.994ThrHis: 0.994 ± 0.043	3.036ThrIle: 3.036 ± 0.08	2.268ThrLys: 2.268 ± 0.065	4.873ThrLeu: 4.873 ± 0.101	1.198ThrMet: 1.198 ± 0.044	1.84ThrAsn: 1.84 ± 0.061	2.534ThrPro: 2.534 ± 0.072	1.247ThrGln: 1.247 ± 0.043	2.314ThrArg: 2.314 ± 0.061	2.578ThrSer: 2.578 ± 0.076	2.472ThrThr: 2.472 ± 0.069	5.351ThrVal: 5.351 ± 0.11	0.33ThrTrp: 0.33 ± 0.027	1.666ThrTyr: 1.666 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	5.032ValAla: 5.032 ± 0.105	1.526ValCys: 1.526 ± 0.052	3.805ValAsp: 3.805 ± 0.094	4.272ValGlu: 4.272 ± 0.087	3.513ValPhe: 3.513 ± 0.093	4.449ValGly: 4.449 ± 0.111	1.262ValHis: 1.262 ± 0.048	4.656ValIle: 4.656 ± 0.092	3.253ValLys: 3.253 ± 0.085	6.85ValLeu: 6.85 ± 0.141	1.659ValMet: 1.659 ± 0.054	3.109ValAsn: 3.109 ± 0.071	2.881ValPro: 2.881 ± 0.073	1.732ValGln: 1.732 ± 0.053	4.089ValArg: 4.089 ± 0.083	4.886ValSer: 4.886 ± 0.092	3.923ValThr: 3.923 ± 0.107	4.525ValVal: 4.525 ± 0.095	0.474ValTrp: 0.474 ± 0.031	2.516ValTyr: 2.516 ± 0.073	0.0ValXaa: 0.0 ± 0.0
Trp	0.527TrpAla: 0.527 ± 0.032	0.135TrpCys: 0.135 ± 0.015	0.425TrpAsp: 0.425 ± 0.03	0.458TrpGlu: 0.458 ± 0.032	0.348TrpPhe: 0.348 ± 0.027	0.5TrpGly: 0.5 ± 0.034	0.182TrpHis: 0.182 ± 0.02	0.443TrpIle: 0.443 ± 0.031	0.363TrpLys: 0.363 ± 0.03	0.753TrpLeu: 0.753 ± 0.038	0.221TrpMet: 0.221 ± 0.018	0.365TrpAsn: 0.365 ± 0.026	0.135TrpPro: 0.135 ± 0.016	0.308TrpGln: 0.308 ± 0.026	0.388TrpArg: 0.388 ± 0.024	0.399TrpSer: 0.399 ± 0.029	0.374TrpThr: 0.374 ± 0.03	0.443TrpVal: 0.443 ± 0.032	0.078TrpTrp: 0.078 ± 0.014	0.264TrpTyr: 0.264 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.041TyrAla: 3.041 ± 0.072	0.711TyrCys: 0.711 ± 0.033	2.348TyrAsp: 2.348 ± 0.072	2.126TyrGlu: 2.126 ± 0.071	1.584TyrPhe: 1.584 ± 0.057	2.653TyrGly: 2.653 ± 0.075	0.562TyrHis: 0.562 ± 0.031	2.5TyrIle: 2.5 ± 0.076	1.57TyrLys: 1.57 ± 0.06	2.8TyrLeu: 2.8 ± 0.078	0.881TyrMet: 0.881 ± 0.047	1.588TyrAsn: 1.588 ± 0.062	1.236TyrPro: 1.236 ± 0.044	0.7TyrGln: 0.7 ± 0.034	1.978TyrArg: 1.978 ± 0.064	2.478TyrSer: 2.478 ± 0.079	2.051TyrThr: 2.051 ± 0.07	2.157TyrVal: 2.157 ± 0.07	0.263TyrTrp: 0.263 ± 0.025	1.462TyrTyr: 1.462 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.002XaaCys: 0.002 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.002	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.033XaaXaa: 0.033 ± 0.012

Statistics based on 1746 proteins (548493 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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