Amino acid dipepetide frequency for Clostridium pasteurianum DSM 525 = ATCC 6013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.401AlaAla: 4.401 ± 0.088	0.724AlaCys: 0.724 ± 0.027	2.735AlaAsp: 2.735 ± 0.06	3.584AlaGlu: 3.584 ± 0.063	2.624AlaPhe: 2.624 ± 0.057	3.863AlaGly: 3.863 ± 0.071	0.842AlaHis: 0.842 ± 0.026	5.419AlaIle: 5.419 ± 0.073	4.636AlaLys: 4.636 ± 0.067	5.678AlaLeu: 5.678 ± 0.083	1.636AlaMet: 1.636 ± 0.038	2.594AlaAsn: 2.594 ± 0.043	1.386AlaPro: 1.386 ± 0.041	1.406AlaGln: 1.406 ± 0.038	1.856AlaArg: 1.856 ± 0.044	3.518AlaSer: 3.518 ± 0.059	2.391AlaThr: 2.391 ± 0.058	4.427AlaVal: 4.427 ± 0.07	0.366AlaTrp: 0.366 ± 0.019	2.112AlaTyr: 2.112 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.708CysAla: 0.708 ± 0.026	0.204CysCys: 0.204 ± 0.014	0.624CysAsp: 0.624 ± 0.022	0.799CysGlu: 0.799 ± 0.027	0.481CysPhe: 0.481 ± 0.02	1.103CysGly: 1.103 ± 0.036	0.207CysHis: 0.207 ± 0.013	1.164CysIle: 1.164 ± 0.031	0.998CysLys: 0.998 ± 0.03	0.861CysLeu: 0.861 ± 0.027	0.275CysMet: 0.275 ± 0.015	0.773CysAsn: 0.773 ± 0.029	0.469CysPro: 0.469 ± 0.021	0.206CysGln: 0.206 ± 0.012	0.425CysArg: 0.425 ± 0.019	0.831CysSer: 0.831 ± 0.027	0.639CysThr: 0.639 ± 0.026	0.614CysVal: 0.614 ± 0.025	0.082CysTrp: 0.082 ± 0.009	0.431CysTyr: 0.431 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.741AspAla: 2.741 ± 0.055	0.605AspCys: 0.605 ± 0.024	2.482AspAsp: 2.482 ± 0.053	4.104AspGlu: 4.104 ± 0.053	2.73AspPhe: 2.73 ± 0.06	3.272AspGly: 3.272 ± 0.063	0.606AspHis: 0.606 ± 0.025	6.622AspIle: 6.622 ± 0.096	5.465AspLys: 5.465 ± 0.077	4.724AspLeu: 4.724 ± 0.065	1.541AspMet: 1.541 ± 0.036	3.614AspAsn: 3.614 ± 0.068	1.349AspPro: 1.349 ± 0.044	0.853AspGln: 0.853 ± 0.025	1.905AspArg: 1.905 ± 0.044	3.416AspSer: 3.416 ± 0.061	2.594AspThr: 2.594 ± 0.052	3.415AspVal: 3.415 ± 0.048	0.373AspTrp: 0.373 ± 0.018	2.572AspTyr: 2.572 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	3.998GluAla: 3.998 ± 0.066	0.633GluCys: 0.633 ± 0.026	4.313GluAsp: 4.313 ± 0.074	6.116GluGlu: 6.116 ± 0.105	2.897GluPhe: 2.897 ± 0.055	3.728GluGly: 3.728 ± 0.062	0.908GluHis: 0.908 ± 0.025	6.926GluIle: 6.926 ± 0.09	7.46GluLys: 7.46 ± 0.099	6.257GluLeu: 6.257 ± 0.083	1.702GluMet: 1.702 ± 0.044	5.359GluAsn: 5.359 ± 0.076	1.329GluPro: 1.329 ± 0.034	1.737GluGln: 1.737 ± 0.044	2.495GluArg: 2.495 ± 0.058	3.406GluSer: 3.406 ± 0.054	2.763GluThr: 2.763 ± 0.053	4.198GluVal: 4.198 ± 0.06	0.451GluTrp: 0.451 ± 0.021	2.904GluTyr: 2.904 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.27PheAla: 2.27 ± 0.048	0.551PheCys: 0.551 ± 0.023	2.432PheAsp: 2.432 ± 0.053	2.444PheGlu: 2.444 ± 0.054	2.041PhePhe: 2.041 ± 0.047	2.858PheGly: 2.858 ± 0.052	0.662PheHis: 0.662 ± 0.023	4.941PheIle: 4.941 ± 0.081	3.956PheLys: 3.956 ± 0.061	3.95PheLeu: 3.95 ± 0.071	1.203PheMet: 1.203 ± 0.031	3.046PheAsn: 3.046 ± 0.057	1.191PhePro: 1.191 ± 0.032	1.135PheGln: 1.135 ± 0.031	1.362PheArg: 1.362 ± 0.033	3.296PheSer: 3.296 ± 0.051	2.313PheThr: 2.313 ± 0.047	2.678PheVal: 2.678 ± 0.05	0.337PheTrp: 0.337 ± 0.016	1.907PheTyr: 1.907 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	3.824GlyAla: 3.824 ± 0.064	0.97GlyCys: 0.97 ± 0.033	3.123GlyAsp: 3.123 ± 0.053	3.877GlyGlu: 3.877 ± 0.056	2.966GlyPhe: 2.966 ± 0.056	4.112GlyGly: 4.112 ± 0.078	1.04GlyHis: 1.04 ± 0.032	7.106GlyIle: 7.106 ± 0.091	5.698GlyLys: 5.698 ± 0.071	5.076GlyLeu: 5.076 ± 0.06	1.723GlyMet: 1.723 ± 0.042	3.545GlyAsn: 3.545 ± 0.067	1.197GlyPro: 1.197 ± 0.033	1.359GlyGln: 1.359 ± 0.04	2.132GlyArg: 2.132 ± 0.042	3.71GlySer: 3.71 ± 0.058	3.438GlyThr: 3.438 ± 0.057	4.159GlyVal: 4.159 ± 0.069	0.517GlyTrp: 0.517 ± 0.023	2.889GlyTyr: 2.889 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	0.69HisAla: 0.69 ± 0.027	0.222HisCys: 0.222 ± 0.014	0.653HisAsp: 0.653 ± 0.026	0.876HisGlu: 0.876 ± 0.027	0.645HisPhe: 0.645 ± 0.025	0.986HisGly: 0.986 ± 0.034	0.31HisHis: 0.31 ± 0.019	1.453HisIle: 1.453 ± 0.038	1.171HisLys: 1.171 ± 0.032	1.137HisLeu: 1.137 ± 0.028	0.393HisMet: 0.393 ± 0.019	0.873HisAsn: 0.873 ± 0.027	0.622HisPro: 0.622 ± 0.024	0.314HisGln: 0.314 ± 0.016	0.562HisArg: 0.562 ± 0.02	0.977HisSer: 0.977 ± 0.031	0.686HisThr: 0.686 ± 0.024	0.793HisVal: 0.793 ± 0.024	0.13HisTrp: 0.13 ± 0.011	0.615HisTyr: 0.615 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.903IleAla: 5.903 ± 0.086	1.294IleCys: 1.294 ± 0.037	6.066IleAsp: 6.066 ± 0.08	6.96IleGlu: 6.96 ± 0.083	4.629IlePhe: 4.629 ± 0.074	6.227IleGly: 6.227 ± 0.071	1.383IleHis: 1.383 ± 0.035	10.4IleIle: 10.4 ± 0.135	9.269IleLys: 9.269 ± 0.097	9.415IleLeu: 9.415 ± 0.112	2.424IleMet: 2.424 ± 0.048	6.879IleAsn: 6.879 ± 0.085	3.498IlePro: 3.498 ± 0.059	2.328IleGln: 2.328 ± 0.049	3.191IleArg: 3.191 ± 0.056	7.391IleSer: 7.391 ± 0.093	5.022IleThr: 5.022 ± 0.073	6.329IleVal: 6.329 ± 0.075	0.622IleTrp: 0.622 ± 0.023	3.925IleTyr: 3.925 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	5.035LysAla: 5.035 ± 0.078	0.906LysCys: 0.906 ± 0.035	6.039LysAsp: 6.039 ± 0.083	7.64LysGlu: 7.64 ± 0.096	3.564LysPhe: 3.564 ± 0.057	5.056LysGly: 5.056 ± 0.068	1.124LysHis: 1.124 ± 0.029	8.91LysIle: 8.91 ± 0.102	8.447LysLys: 8.447 ± 0.108	7.934LysLeu: 7.934 ± 0.091	2.303LysMet: 2.303 ± 0.04	7.188LysAsn: 7.188 ± 0.087	2.211LysPro: 2.211 ± 0.044	2.228LysGln: 2.228 ± 0.046	3.141LysArg: 3.141 ± 0.052	5.559LysSer: 5.559 ± 0.08	4.011LysThr: 4.011 ± 0.062	5.671LysVal: 5.671 ± 0.071	0.673LysTrp: 0.673 ± 0.028	4.262LysTyr: 4.262 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	4.752LeuAla: 4.752 ± 0.057	1.056LeuCys: 1.056 ± 0.029	4.757LeuAsp: 4.757 ± 0.066	5.752LeuGlu: 5.752 ± 0.076	3.642LeuPhe: 3.642 ± 0.061	5.586LeuGly: 5.586 ± 0.072	1.146LeuHis: 1.146 ± 0.031	8.549LeuIle: 8.549 ± 0.098	9.016LeuLys: 9.016 ± 0.094	7.5LeuLeu: 7.5 ± 0.105	2.256LeuMet: 2.256 ± 0.043	5.944LeuAsn: 5.944 ± 0.068	2.792LeuPro: 2.792 ± 0.047	2.276LeuGln: 2.276 ± 0.045	3.129LeuArg: 3.129 ± 0.052	6.656LeuSer: 6.656 ± 0.071	4.266LeuThr: 4.266 ± 0.065	4.877LeuVal: 4.877 ± 0.072	0.594LeuTrp: 0.594 ± 0.025	3.285LeuTyr: 3.285 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.671MetAla: 1.671 ± 0.041	0.252MetCys: 0.252 ± 0.015	1.693MetAsp: 1.693 ± 0.039	1.858MetGlu: 1.858 ± 0.043	0.963MetPhe: 0.963 ± 0.031	1.727MetGly: 1.727 ± 0.04	0.348MetHis: 0.348 ± 0.017	2.186MetIle: 2.186 ± 0.042	2.442MetLys: 2.442 ± 0.047	2.209MetLeu: 2.209 ± 0.045	0.612MetMet: 0.612 ± 0.029	1.72MetAsn: 1.72 ± 0.037	0.86MetPro: 0.86 ± 0.03	0.622MetGln: 0.622 ± 0.025	0.842MetArg: 0.842 ± 0.028	1.714MetSer: 1.714 ± 0.041	1.18MetThr: 1.18 ± 0.032	1.439MetVal: 1.439 ± 0.03	0.155MetTrp: 0.155 ± 0.012	0.878MetTyr: 0.878 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.079AsnAla: 3.079 ± 0.057	0.796AsnCys: 0.796 ± 0.026	2.955AsnAsp: 2.955 ± 0.05	3.948AsnGlu: 3.948 ± 0.064	2.987AsnPhe: 2.987 ± 0.052	3.603AsnGly: 3.603 ± 0.062	0.83AsnHis: 0.83 ± 0.026	7.935AsnIle: 7.935 ± 0.089	6.248AsnLys: 6.248 ± 0.073	5.726AsnLeu: 5.726 ± 0.084	1.779AsnMet: 1.779 ± 0.035	4.976AsnAsn: 4.976 ± 0.102	2.156AsnPro: 2.156 ± 0.043	1.409AsnGln: 1.409 ± 0.037	2.344AsnArg: 2.344 ± 0.046	4.611AsnSer: 4.611 ± 0.071	3.434AsnThr: 3.434 ± 0.063	3.675AsnVal: 3.675 ± 0.06	0.495AsnTrp: 0.495 ± 0.02	3.002AsnTyr: 3.002 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	1.496ProAla: 1.496 ± 0.039	0.326ProCys: 0.326 ± 0.019	1.542ProAsp: 1.542 ± 0.038	2.269ProGlu: 2.269 ± 0.044	1.439ProPhe: 1.439 ± 0.037	1.71ProGly: 1.71 ± 0.038	0.459ProHis: 0.459 ± 0.021	2.683ProIle: 2.683 ± 0.054	2.327ProLys: 2.327 ± 0.043	2.546ProLeu: 2.546 ± 0.051	0.668ProMet: 0.668 ± 0.024	1.489ProAsn: 1.489 ± 0.045	0.65ProPro: 0.65 ± 0.023	0.808ProGln: 0.808 ± 0.024	0.891ProArg: 0.891 ± 0.031	1.805ProSer: 1.805 ± 0.042	1.364ProThr: 1.364 ± 0.035	2.217ProVal: 2.217 ± 0.043	0.268ProTrp: 0.268 ± 0.017	1.309ProTyr: 1.309 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	1.328GlnAla: 1.328 ± 0.038	0.301GlnCys: 0.301 ± 0.015	1.221GlnAsp: 1.221 ± 0.034	1.643GlnGlu: 1.643 ± 0.043	1.055GlnPhe: 1.055 ± 0.032	1.555GlnGly: 1.555 ± 0.043	0.407GlnHis: 0.407 ± 0.017	2.171GlnIle: 2.171 ± 0.047	2.137GlnLys: 2.137 ± 0.046	2.17GlnLeu: 2.17 ± 0.044	0.651GlnMet: 0.651 ± 0.025	1.497GlnAsn: 1.497 ± 0.038	0.588GlnPro: 0.588 ± 0.023	0.857GlnGln: 0.857 ± 0.029	0.983GlnArg: 0.983 ± 0.028	1.472GlnSer: 1.472 ± 0.034	1.061GlnThr: 1.061 ± 0.033	1.477GlnVal: 1.477 ± 0.035	0.26GlnTrp: 0.26 ± 0.016	1.123GlnTyr: 1.123 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	1.822ArgAla: 1.822 ± 0.039	0.397ArgCys: 0.397 ± 0.022	1.991ArgAsp: 1.991 ± 0.044	3.07ArgGlu: 3.07 ± 0.054	1.47ArgPhe: 1.47 ± 0.032	1.988ArgGly: 1.988 ± 0.045	0.53ArgHis: 0.53 ± 0.02	3.344ArgIle: 3.344 ± 0.054	3.129ArgLys: 3.129 ± 0.058	3.0ArgLeu: 3.0 ± 0.052	0.882ArgMet: 0.882 ± 0.03	2.225ArgAsn: 2.225 ± 0.042	0.921ArgPro: 0.921 ± 0.032	0.956ArgGln: 0.956 ± 0.029	1.483ArgArg: 1.483 ± 0.041	1.758ArgSer: 1.758 ± 0.041	1.572ArgThr: 1.572 ± 0.037	2.065ArgVal: 2.065 ± 0.037	0.284ArgTrp: 0.284 ± 0.017	1.493ArgTyr: 1.493 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.356SerAla: 3.356 ± 0.053	0.707SerCys: 0.707 ± 0.025	3.293SerAsp: 3.293 ± 0.055	4.247SerGlu: 4.247 ± 0.063	3.087SerPhe: 3.087 ± 0.053	4.456SerGly: 4.456 ± 0.074	0.958SerHis: 0.958 ± 0.031	7.267SerIle: 7.267 ± 0.096	6.054SerLys: 6.054 ± 0.075	5.674SerLeu: 5.674 ± 0.069	1.638SerMet: 1.638 ± 0.04	4.147SerAsn: 4.147 ± 0.075	1.8SerPro: 1.8 ± 0.035	1.735SerGln: 1.735 ± 0.039	2.235SerArg: 2.235 ± 0.045	4.693SerSer: 4.693 ± 0.093	3.31SerThr: 3.31 ± 0.066	3.947SerVal: 3.947 ± 0.067	0.479SerTrp: 0.479 ± 0.017	2.606SerTyr: 2.606 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	3.248ThrAla: 3.248 ± 0.055	0.522ThrCys: 0.522 ± 0.019	2.553ThrAsp: 2.553 ± 0.051	3.033ThrGlu: 3.033 ± 0.053	2.165ThrPhe: 2.165 ± 0.044	3.652ThrGly: 3.652 ± 0.071	0.707ThrHis: 0.707 ± 0.028	4.822ThrIle: 4.822 ± 0.071	3.64ThrLys: 3.64 ± 0.06	4.38ThrLeu: 4.38 ± 0.068	1.042ThrMet: 1.042 ± 0.027	2.637ThrAsn: 2.637 ± 0.053	1.794ThrPro: 1.794 ± 0.044	1.081ThrGln: 1.081 ± 0.03	1.534ThrArg: 1.534 ± 0.033	3.21ThrSer: 3.21 ± 0.06	2.613ThrThr: 2.613 ± 0.061	3.385ThrVal: 3.385 ± 0.056	0.362ThrTrp: 0.362 ± 0.019	1.905ThrTyr: 1.905 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	3.473ValAla: 3.473 ± 0.056	0.801ValCys: 0.801 ± 0.027	3.669ValAsp: 3.669 ± 0.059	4.163ValGlu: 4.163 ± 0.064	2.802ValPhe: 2.802 ± 0.047	3.907ValGly: 3.907 ± 0.065	0.897ValHis: 0.897 ± 0.027	6.231ValIle: 6.231 ± 0.07	5.49ValLys: 5.49 ± 0.072	5.489ValLeu: 5.489 ± 0.067	1.41ValMet: 1.41 ± 0.035	3.893ValAsn: 3.893 ± 0.068	2.045ValPro: 2.045 ± 0.048	1.554ValGln: 1.554 ± 0.043	1.957ValArg: 1.957 ± 0.043	4.284ValSer: 4.284 ± 0.069	3.193ValThr: 3.193 ± 0.055	4.168ValVal: 4.168 ± 0.071	0.421ValTrp: 0.421 ± 0.018	2.399ValTyr: 2.399 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.4TrpAla: 0.4 ± 0.022	0.09TrpCys: 0.09 ± 0.008	0.38TrpAsp: 0.38 ± 0.021	0.409TrpGlu: 0.409 ± 0.02	0.35TrpPhe: 0.35 ± 0.018	0.519TrpGly: 0.519 ± 0.023	0.162TrpHis: 0.162 ± 0.012	0.711TrpIle: 0.711 ± 0.024	0.624TrpLys: 0.624 ± 0.023	0.615TrpLeu: 0.615 ± 0.027	0.195TrpMet: 0.195 ± 0.012	0.516TrpAsn: 0.516 ± 0.023	0.172TrpPro: 0.172 ± 0.015	0.251TrpGln: 0.251 ± 0.017	0.269TrpArg: 0.269 ± 0.018	0.453TrpSer: 0.453 ± 0.021	0.346TrpThr: 0.346 ± 0.021	0.4TrpVal: 0.4 ± 0.018	0.104TrpTrp: 0.104 ± 0.011	0.29TrpTyr: 0.29 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.005TyrAla: 2.005 ± 0.043	0.536TyrCys: 0.536 ± 0.024	2.46TyrAsp: 2.46 ± 0.053	2.786TyrGlu: 2.786 ± 0.051	2.035TyrPhe: 2.035 ± 0.043	2.554TyrGly: 2.554 ± 0.05	0.583TyrHis: 0.583 ± 0.023	4.316TyrIle: 4.316 ± 0.066	3.699TyrLys: 3.699 ± 0.063	3.491TyrLeu: 3.491 ± 0.059	1.039TyrMet: 1.039 ± 0.03	3.071TyrAsn: 3.071 ± 0.058	1.234TyrPro: 1.234 ± 0.031	0.837TyrGln: 0.837 ± 0.035	1.647TyrArg: 1.647 ± 0.04	2.93TyrSer: 2.93 ± 0.051	2.087TyrThr: 2.087 ± 0.042	2.312TyrVal: 2.312 ± 0.042	0.286TyrTrp: 0.286 ± 0.017	1.969TyrTyr: 1.969 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3930 proteins (1177515 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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