Candidatus Thioglobus singularis PS1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Pseudothioglobus; Candidatus Pseudothioglobus singularis

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3T2H1|A0A0M3T2H1_9GAMM Uncharacterized protein OS=Candidatus Thioglobus singularis PS1 OX=1125411 GN=W908_05475 PE=4 SV=1
MM1 pKa = 7.37QINIISKK8 pKa = 10.69LITPVLLIVFLTSCSPSNVEE28 pKa = 3.6VSIYY32 pKa = 8.15TTDD35 pKa = 3.07IDD37 pKa = 3.97VAQEE41 pKa = 4.4GEE43 pKa = 4.41VLEE46 pKa = 4.53VPVMASFSLYY56 pKa = 10.64SDD58 pKa = 4.28DD59 pKa = 5.9DD60 pKa = 4.61GEE62 pKa = 4.88LEE64 pKa = 4.27SASQIAEE71 pKa = 4.24KK72 pKa = 10.87YY73 pKa = 9.12LAPDD77 pKa = 4.09SIFSQSSGDD86 pKa = 3.23WGEE89 pKa = 4.04TLVIEE94 pKa = 4.52TTIPMGTLDD103 pKa = 4.01SLQNYY108 pKa = 7.44LASNNRR114 pKa = 11.84VAVLLVQGVDD124 pKa = 3.62EE125 pKa = 4.88IEE127 pKa = 4.38ISLSSTDD134 pKa = 3.53YY135 pKa = 11.76ADD137 pKa = 4.07ALNSEE142 pKa = 4.35LTDD145 pKa = 3.38INFMLGFEE153 pKa = 4.79LPGDD157 pKa = 3.75STNFRR162 pKa = 11.84VISDD166 pKa = 3.5SRR168 pKa = 11.84NEE170 pKa = 4.0VQVDD174 pKa = 3.67ATAVFVSEE182 pKa = 4.81KK183 pKa = 10.28PYY185 pKa = 10.72LYY187 pKa = 10.23YY188 pKa = 10.99SKK190 pKa = 10.78VLKK193 pKa = 10.59RR194 pKa = 11.84RR195 pKa = 11.84DD196 pKa = 3.16EE197 pKa = 4.16AEE199 pKa = 3.79IVFKK203 pKa = 10.45GTTDD207 pKa = 3.14SVYY210 pKa = 11.11SEE212 pKa = 4.67INPLIYY218 pKa = 10.02INYY221 pKa = 8.19PP222 pKa = 3.14

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3T1N4|A0A0M3T1N4_9GAMM Probable GTP-binding protein EngB OS=Candidatus Thioglobus singularis PS1 OX=1125411 GN=engB PE=3 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84IAGINIPTNKK13 pKa = 9.87HH14 pKa = 4.63IVIGLQSIFGIGEE27 pKa = 4.0TRR29 pKa = 11.84SKK31 pKa = 10.49QICEE35 pKa = 3.95ALKK38 pKa = 10.53LDD40 pKa = 4.21PSTKK44 pKa = 10.09VSALTEE50 pKa = 4.22DD51 pKa = 3.34QLEE54 pKa = 4.61SIRR57 pKa = 11.84TAVAQYY63 pKa = 10.2EE64 pKa = 4.51VEE66 pKa = 4.09GDD68 pKa = 3.55LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.7VAMSIKK78 pKa = 10.33RR79 pKa = 11.84LRR81 pKa = 11.84DD82 pKa = 3.19LGCYY86 pKa = 9.55RR87 pKa = 11.84GIRR90 pKa = 11.84HH91 pKa = 6.47RR92 pKa = 11.84KK93 pKa = 8.06GLPLRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.83TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.41GPRR113 pKa = 11.84RR114 pKa = 11.84LIKK117 pKa = 10.58

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1681

0

1681

526563

32

1505

313.2

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.095 ± 0.063

1.054 ± 0.02

5.679 ± 0.046

6.412 ± 0.047

4.521 ± 0.05

6.91 ± 0.059

1.981 ± 0.024

8.051 ± 0.054

6.654 ± 0.058

9.792 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.029

5.229 ± 0.047

3.584 ± 0.033

3.301 ± 0.032

3.712 ± 0.036

7.795 ± 0.054

4.888 ± 0.04

6.505 ± 0.054

1.094 ± 0.025

3.068 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski