Circovirus-like genome DHCV-3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHE3|A0A190WHE3_9CIRC Uncharacterized protein OS=Circovirus-like genome DHCV-3 OX=1788452 PE=4 SV=1
MM1 pKa = 7.94AEE3 pKa = 4.14VNYY6 pKa = 9.81HH7 pKa = 5.82APKK10 pKa = 10.07NLHH13 pKa = 7.05LIPIGVPYY21 pKa = 11.01VSMLSIHH28 pKa = 6.33FLVAPLSYY36 pKa = 10.55NNSMHH41 pKa = 7.09LGGFHH46 pKa = 7.45LLFLYY51 pKa = 10.85VPDD54 pKa = 4.62LVTISSFSKK63 pKa = 10.7NFSHH67 pKa = 6.71SQNIHH72 pKa = 4.65NTVNALFLFPLPLYY86 pKa = 10.23QSQHH90 pKa = 5.36TDD92 pKa = 2.84TLQIIPGIPCNHH104 pKa = 6.29HH105 pKa = 6.74RR106 pKa = 11.84YY107 pKa = 10.04NYY109 pKa = 10.66

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHG0|A0A190WHG0_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DHCV-3 OX=1788452 PE=3 SV=1
MM1 pKa = 7.65AYY3 pKa = 9.68KK4 pKa = 10.52RR5 pKa = 11.84NRR7 pKa = 11.84RR8 pKa = 11.84GGRR11 pKa = 11.84KK12 pKa = 7.56TNVKK16 pKa = 9.92KK17 pKa = 10.57AVRR20 pKa = 11.84ANNRR24 pKa = 11.84ARR26 pKa = 11.84QYY28 pKa = 10.23NAPAKK33 pKa = 8.67VASVVSLAKK42 pKa = 10.24SVNRR46 pKa = 11.84LKK48 pKa = 10.35TKK50 pKa = 8.21MRR52 pKa = 11.84RR53 pKa = 11.84EE54 pKa = 4.0TEE56 pKa = 3.93LKK58 pKa = 10.13EE59 pKa = 4.16FEE61 pKa = 4.46SGLLSTTVGQVNINNTGMYY80 pKa = 9.71IDD82 pKa = 5.79DD83 pKa = 5.53LDD85 pKa = 5.21LMDD88 pKa = 4.26IAEE91 pKa = 4.55GASDD95 pKa = 3.39KK96 pKa = 11.0TRR98 pKa = 11.84IGKK101 pKa = 9.44KK102 pKa = 10.25VMLKK106 pKa = 10.07GVQVRR111 pKa = 11.84LQFSHH116 pKa = 6.4QSNTSSASNYY126 pKa = 9.34IIEE129 pKa = 4.38VYY131 pKa = 8.89KK132 pKa = 9.88TQDD135 pKa = 2.95FGSTLTAIRR144 pKa = 11.84DD145 pKa = 3.49ALYY148 pKa = 10.87NVDD151 pKa = 4.74SISGVIDD158 pKa = 3.42YY159 pKa = 11.22SSTHH163 pKa = 5.87KK164 pKa = 11.12NEE166 pKa = 3.83AKK168 pKa = 10.16SWLKK172 pKa = 9.87RR173 pKa = 11.84VAYY176 pKa = 9.47KK177 pKa = 10.57RR178 pKa = 11.84IRR180 pKa = 11.84LPVDD184 pKa = 3.44TFTGVNMVKK193 pKa = 10.14DD194 pKa = 3.53VKK196 pKa = 10.71MFIKK200 pKa = 10.29QNQEE204 pKa = 3.16LTYY207 pKa = 9.91TLGSTQVPQNYY218 pKa = 9.54RR219 pKa = 11.84YY220 pKa = 9.6IICIRR225 pKa = 11.84SSVGNNNATTASTLTTVVHH244 pKa = 5.6TTALTGAIVRR254 pKa = 11.84LVSKK258 pKa = 10.32CWYY261 pKa = 8.38TDD263 pKa = 2.91NN264 pKa = 5.04

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

663

109

290

221.0

25.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.581 ± 0.831

1.357 ± 0.419

4.525 ± 0.772

5.128 ± 2.073

2.866 ± 0.913

5.43 ± 0.546

2.866 ± 1.619

5.581 ± 0.538

7.692 ± 1.555

7.994 ± 1.478

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.866 ± 0.141

6.637 ± 1.094

3.318 ± 1.394

3.771 ± 0.026

5.882 ± 1.272

7.24 ± 1.017

6.637 ± 1.634

7.391 ± 1.093

1.659 ± 0.854

5.581 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski