Hypericum japonicum associated circular DNA virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus hypas1; Hypericum associated gemycircularvirus 1

Average proteome isoelectric point is 7.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5S0Z8|S5S0Z8_9VIRU Putative V2 protein OS=Hypericum japonicum associated circular DNA virus OX=1367218 PE=4 SV=1
MM1 pKa = 7.51PQLEE5 pKa = 4.38WNFRR9 pKa = 11.84YY10 pKa = 10.49VLVTYY15 pKa = 7.86AQCGDD20 pKa = 3.91LDD22 pKa = 3.45PWRR25 pKa = 11.84VVEE28 pKa = 4.5RR29 pKa = 11.84FSSLGAEE36 pKa = 4.2CIVGRR41 pKa = 11.84EE42 pKa = 3.95HH43 pKa = 8.11HH44 pKa = 6.88EE45 pKa = 4.43DD46 pKa = 3.53GGLHH50 pKa = 5.69LHH52 pKa = 6.24VFADD56 pKa = 4.7FGRR59 pKa = 11.84KK60 pKa = 8.18FRR62 pKa = 11.84SRR64 pKa = 11.84KK65 pKa = 9.81ADD67 pKa = 3.2ILDD70 pKa = 3.23VDD72 pKa = 3.98GRR74 pKa = 11.84HH75 pKa = 6.5PNLAPIKK82 pKa = 8.35RR83 pKa = 11.84TPAKK87 pKa = 10.37AYY89 pKa = 10.34DD90 pKa = 3.71YY91 pKa = 10.64AIKK94 pKa = 10.92DD95 pKa = 3.52GDD97 pKa = 4.01VVAGGLEE104 pKa = 3.95RR105 pKa = 11.84PVEE108 pKa = 4.29GGVGNGTSADD118 pKa = 2.91KK119 pKa = 10.31WSRR122 pKa = 11.84ITQAEE127 pKa = 4.09DD128 pKa = 3.68RR129 pKa = 11.84DD130 pKa = 4.22EE131 pKa = 4.72FWALVHH137 pKa = 6.48EE138 pKa = 5.38LDD140 pKa = 4.56PKK142 pKa = 10.9AAACSFNALSKK153 pKa = 9.33YY154 pKa = 10.43ADD156 pKa = 2.87WRR158 pKa = 11.84FAEE161 pKa = 4.39KK162 pKa = 10.48PPVYY166 pKa = 9.95EE167 pKa = 3.52HH168 pKa = 7.52DD169 pKa = 3.54GRR171 pKa = 11.84IEE173 pKa = 4.01FVPGDD178 pKa = 3.54ADD180 pKa = 5.19GRR182 pKa = 11.84DD183 pKa = 3.5DD184 pKa = 3.59WVSQSGIGLDD194 pKa = 3.7EE195 pKa = 4.64PFLGGRR201 pKa = 11.84VKK203 pKa = 10.9SLVLFGGTRR212 pKa = 11.84TGKK215 pKa = 5.99TTWARR220 pKa = 11.84SLGKK224 pKa = 9.48HH225 pKa = 6.3LYY227 pKa = 10.6CIGLVSGAEE236 pKa = 4.08CSKK239 pKa = 11.2GADD242 pKa = 2.93AEE244 pKa = 4.49YY245 pKa = 10.97AVFDD249 pKa = 5.21DD250 pKa = 3.48IRR252 pKa = 11.84GGFGFFHH259 pKa = 7.53GYY261 pKa = 9.92KK262 pKa = 9.15EE263 pKa = 3.99WLGAQPHH270 pKa = 5.31VSIKK274 pKa = 9.68QLYY277 pKa = 8.59RR278 pKa = 11.84EE279 pKa = 4.34PYY281 pKa = 7.28YY282 pKa = 10.19MKK284 pKa = 9.88WGKK287 pKa = 9.51PSIWICNTDD296 pKa = 3.34PRR298 pKa = 11.84LDD300 pKa = 3.53AYY302 pKa = 10.49GPNATPDD309 pKa = 3.75WEE311 pKa = 4.2WMEE314 pKa = 4.4GNCHH318 pKa = 6.54FIEE321 pKa = 4.61VKK323 pKa = 10.61DD324 pKa = 3.86SLISSISHH332 pKa = 7.12ASTEE336 pKa = 4.06

Molecular weight: 37.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5S0Z8|S5S0Z8_9VIRU Putative V2 protein OS=Hypericum japonicum associated circular DNA virus OX=1367218 PE=4 SV=1
MM1 pKa = 7.79APMLVILLHH10 pKa = 6.93CYY12 pKa = 10.31LKK14 pKa = 10.86LLCTGMRR21 pKa = 11.84NRR23 pKa = 11.84RR24 pKa = 11.84DD25 pKa = 3.34KK26 pKa = 11.09RR27 pKa = 11.84VLDD30 pKa = 4.1FNEE33 pKa = 3.99MTIPLHH39 pKa = 5.85PFPIRR44 pKa = 11.84RR45 pKa = 11.84SVRR48 pKa = 11.84AIGIQTRR55 pKa = 11.84VGVANPNRR63 pKa = 11.84RR64 pKa = 11.84LTPLHH69 pKa = 6.27VVGLPVKK76 pKa = 10.64LLDD79 pKa = 3.92GYY81 pKa = 10.24MRR83 pKa = 11.84LSTEE87 pKa = 3.91PLLVSVKK94 pKa = 9.61EE95 pKa = 4.39SKK97 pKa = 7.47TTSDD101 pKa = 3.48VVEE104 pKa = 5.04DD105 pKa = 4.11GVLGVGSLRR114 pKa = 11.84TFRR117 pKa = 11.84TT118 pKa = 3.52

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1052

106

336

210.4

23.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.703 ± 1.19

1.521 ± 0.385

5.513 ± 1.03

5.323 ± 1.34

3.232 ± 0.589

7.319 ± 1.383

2.567 ± 0.691

4.373 ± 0.281

4.183 ± 0.793

9.125 ± 1.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.327 ± 1.041

4.183 ± 1.334

4.848 ± 0.52

2.376 ± 0.469

8.84 ± 0.926

8.365 ± 2.12

6.939 ± 1.667

5.989 ± 1.204

2.852 ± 0.502

3.422 ± 0.872
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski