Amino acid dipepetide frequency for Duncaniella muris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.747AlaAla: 7.747 ± 0.151
0.903AlaCys: 0.903 ± 0.035
5.779AlaAsp: 5.779 ± 0.085
5.511AlaGlu: 5.511 ± 0.092
3.17AlaPhe: 3.17 ± 0.058
6.063AlaGly: 6.063 ± 0.091
1.298AlaHis: 1.298 ± 0.032
5.513AlaIle: 5.513 ± 0.092
4.156AlaLys: 4.156 ± 0.079
7.623AlaLeu: 7.623 ± 0.115
2.347AlaMet: 2.347 ± 0.052
3.161AlaAsn: 3.161 ± 0.058
3.181AlaPro: 3.181 ± 0.077
2.621AlaGln: 2.621 ± 0.057
4.17AlaArg: 4.17 ± 0.073
5.203AlaSer: 5.203 ± 0.09
4.652AlaThr: 4.652 ± 0.071
5.885AlaVal: 5.885 ± 0.095
0.858AlaTrp: 0.858 ± 0.031
2.894AlaTyr: 2.894 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.031
0.216CysCys: 0.216 ± 0.017
0.74CysAsp: 0.74 ± 0.033
0.719CysGlu: 0.719 ± 0.029
0.547CysPhe: 0.547 ± 0.028
1.155CysGly: 1.155 ± 0.035
0.325CysHis: 0.325 ± 0.017
0.84CysIle: 0.84 ± 0.035
0.567CysLys: 0.567 ± 0.027
1.089CysLeu: 1.089 ± 0.036
0.285CysMet: 0.285 ± 0.02
0.544CysAsn: 0.544 ± 0.024
0.57CysPro: 0.57 ± 0.027
0.374CysGln: 0.374 ± 0.02
0.827CysArg: 0.827 ± 0.033
0.833CysSer: 0.833 ± 0.031
0.619CysThr: 0.619 ± 0.025
0.812CysVal: 0.812 ± 0.032
0.141CysTrp: 0.141 ± 0.013
0.51CysTyr: 0.51 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.551AspAla: 4.551 ± 0.077
0.783AspCys: 0.783 ± 0.03
3.196AspAsp: 3.196 ± 0.065
3.839AspGlu: 3.839 ± 0.067
3.096AspPhe: 3.096 ± 0.06
4.736AspGly: 4.736 ± 0.096
0.963AspHis: 0.963 ± 0.03
4.371AspIle: 4.371 ± 0.07
3.212AspLys: 3.212 ± 0.064
5.015AspLeu: 5.015 ± 0.073
1.66AspMet: 1.66 ± 0.042
2.934AspAsn: 2.934 ± 0.055
2.498AspPro: 2.498 ± 0.064
1.29AspGln: 1.29 ± 0.043
3.482AspArg: 3.482 ± 0.054
4.121AspSer: 4.121 ± 0.09
2.914AspThr: 2.914 ± 0.057
3.574AspVal: 3.574 ± 0.06
0.798AspTrp: 0.798 ± 0.03
2.997AspTyr: 2.997 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
5.382GluAla: 5.382 ± 0.093
0.665GluCys: 0.665 ± 0.026
2.984GluAsp: 2.984 ± 0.066
4.203GluGlu: 4.203 ± 0.094
2.439GluPhe: 2.439 ± 0.051
3.841GluGly: 3.841 ± 0.07
1.055GluHis: 1.055 ± 0.032
4.883GluIle: 4.883 ± 0.078
4.182GluLys: 4.182 ± 0.079
5.591GluLeu: 5.591 ± 0.088
1.866GluMet: 1.866 ± 0.047
3.103GluAsn: 3.103 ± 0.056
1.948GluPro: 1.948 ± 0.048
2.048GluGln: 2.048 ± 0.058
3.491GluArg: 3.491 ± 0.074
3.409GluSer: 3.409 ± 0.063
3.229GluThr: 3.229 ± 0.06
3.889GluVal: 3.889 ± 0.063
0.803GluTrp: 0.803 ± 0.03
2.381GluTyr: 2.381 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.245PheAla: 3.245 ± 0.06
0.639PheCys: 0.639 ± 0.031
2.942PheAsp: 2.942 ± 0.055
2.23PheGlu: 2.23 ± 0.051
1.847PhePhe: 1.847 ± 0.049
3.316PheGly: 3.316 ± 0.072
0.8PheHis: 0.8 ± 0.028
3.018PheIle: 3.018 ± 0.065
1.994PheLys: 1.994 ± 0.048
3.473PheLeu: 3.473 ± 0.068
1.203PheMet: 1.203 ± 0.038
2.326PheAsn: 2.326 ± 0.049
1.7PhePro: 1.7 ± 0.047
0.937PheGln: 0.937 ± 0.03
2.305PheArg: 2.305 ± 0.056
3.199PheSer: 3.199 ± 0.058
2.686PheThr: 2.686 ± 0.056
2.588PheVal: 2.588 ± 0.05
0.507PheTrp: 0.507 ± 0.025
1.679PheTyr: 1.679 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
5.346GlyAla: 5.346 ± 0.086
1.145GlyCys: 1.145 ± 0.04
3.886GlyAsp: 3.886 ± 0.08
4.165GlyGlu: 4.165 ± 0.065
3.119GlyPhe: 3.119 ± 0.058
4.88GlyGly: 4.88 ± 0.1
1.392GlyHis: 1.392 ± 0.042
5.2GlyIle: 5.2 ± 0.087
4.446GlyLys: 4.446 ± 0.069
6.076GlyLeu: 6.076 ± 0.097
2.045GlyMet: 2.045 ± 0.05
3.391GlyAsn: 3.391 ± 0.071
1.557GlyPro: 1.557 ± 0.046
2.014GlyGln: 2.014 ± 0.046
3.932GlyArg: 3.932 ± 0.076
4.695GlySer: 4.695 ± 0.075
3.958GlyThr: 3.958 ± 0.076
5.022GlyVal: 5.022 ± 0.09
1.046GlyTrp: 1.046 ± 0.038
3.091GlyTyr: 3.091 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
1.216HisAla: 1.216 ± 0.033
0.314HisCys: 0.314 ± 0.02
1.087HisAsp: 1.087 ± 0.038
1.016HisGlu: 1.016 ± 0.035
0.845HisPhe: 0.845 ± 0.03
1.347HisGly: 1.347 ± 0.042
0.505HisHis: 0.505 ± 0.028
1.402HisIle: 1.402 ± 0.045
0.897HisLys: 0.897 ± 0.033
1.703HisLeu: 1.703 ± 0.046
0.355HisMet: 0.355 ± 0.022
0.941HisAsn: 0.941 ± 0.034
1.013HisPro: 1.013 ± 0.033
0.48HisGln: 0.48 ± 0.021
1.156HisArg: 1.156 ± 0.037
1.218HisSer: 1.218 ± 0.033
1.016HisThr: 1.016 ± 0.037
0.995HisVal: 0.995 ± 0.033
0.229HisTrp: 0.229 ± 0.015
0.777HisTyr: 0.777 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.067IleAla: 6.067 ± 0.098
0.973IleCys: 0.973 ± 0.033
4.761IleAsp: 4.761 ± 0.083
4.394IleGlu: 4.394 ± 0.072
2.755IlePhe: 2.755 ± 0.064
4.765IleGly: 4.765 ± 0.083
1.206IleHis: 1.206 ± 0.033
4.588IleIle: 4.588 ± 0.08
3.448IleLys: 3.448 ± 0.061
5.46IleLeu: 5.46 ± 0.092
1.554IleMet: 1.554 ± 0.043
3.257IleAsn: 3.257 ± 0.061
3.228IlePro: 3.228 ± 0.05
1.606IleGln: 1.606 ± 0.043
3.585IleArg: 3.585 ± 0.058
5.064IleSer: 5.064 ± 0.078
3.822IleThr: 3.822 ± 0.067
4.526IleVal: 4.526 ± 0.085
0.636IleTrp: 0.636 ± 0.031
2.482IleTyr: 2.482 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.823LysAla: 4.823 ± 0.082
0.513LysCys: 0.513 ± 0.026
3.141LysAsp: 3.141 ± 0.064
3.861LysGlu: 3.861 ± 0.073
2.075LysPhe: 2.075 ± 0.05
3.703LysGly: 3.703 ± 0.062
0.918LysHis: 0.918 ± 0.032
3.667LysIle: 3.667 ± 0.065
3.524LysLys: 3.524 ± 0.067
4.385LysLeu: 4.385 ± 0.076
1.716LysMet: 1.716 ± 0.04
2.555LysAsn: 2.555 ± 0.05
2.083LysPro: 2.083 ± 0.051
1.607LysGln: 1.607 ± 0.046
2.808LysArg: 2.808 ± 0.061
3.324LysSer: 3.324 ± 0.064
3.111LysThr: 3.111 ± 0.058
3.614LysVal: 3.614 ± 0.065
0.698LysTrp: 0.698 ± 0.029
2.118LysTyr: 2.118 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
7.46LeuAla: 7.46 ± 0.11
1.319LeuCys: 1.319 ± 0.04
5.162LeuAsp: 5.162 ± 0.083
4.68LeuGlu: 4.68 ± 0.078
3.596LeuPhe: 3.596 ± 0.069
5.936LeuGly: 5.936 ± 0.084
1.764LeuHis: 1.764 ± 0.04
5.542LeuIle: 5.542 ± 0.113
4.905LeuLys: 4.905 ± 0.075
8.283LeuLeu: 8.283 ± 0.139
2.385LeuMet: 2.385 ± 0.058
4.131LeuAsn: 4.131 ± 0.068
4.288LeuPro: 4.288 ± 0.073
2.658LeuGln: 2.658 ± 0.059
5.598LeuArg: 5.598 ± 0.09
6.947LeuSer: 6.947 ± 0.103
5.921LeuThr: 5.921 ± 0.08
4.972LeuVal: 4.972 ± 0.084
1.044LeuTrp: 1.044 ± 0.035
3.264LeuTyr: 3.264 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.531MetAla: 2.531 ± 0.052
0.243MetCys: 0.243 ± 0.016
1.386MetAsp: 1.386 ± 0.034
1.676MetGlu: 1.676 ± 0.043
0.968MetPhe: 0.968 ± 0.035
1.685MetGly: 1.685 ± 0.045
0.402MetHis: 0.402 ± 0.018
1.655MetIle: 1.655 ± 0.045
2.015MetLys: 2.015 ± 0.045
2.54MetLeu: 2.54 ± 0.054
0.755MetMet: 0.755 ± 0.029
1.239MetAsn: 1.239 ± 0.036
1.247MetPro: 1.247 ± 0.037
0.939MetGln: 0.939 ± 0.035
1.529MetArg: 1.529 ± 0.043
1.748MetSer: 1.748 ± 0.048
1.816MetThr: 1.816 ± 0.04
1.597MetVal: 1.597 ± 0.041
0.258MetTrp: 0.258 ± 0.017
0.704MetTyr: 0.704 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.51AsnAla: 3.51 ± 0.059
0.547AsnCys: 0.547 ± 0.024
2.582AsnAsp: 2.582 ± 0.06
2.416AsnGlu: 2.416 ± 0.047
2.06AsnPhe: 2.06 ± 0.05
3.735AsnGly: 3.735 ± 0.07
0.886AsnHis: 0.886 ± 0.036
3.288AsnIle: 3.288 ± 0.063
2.269AsnLys: 2.269 ± 0.051
4.04AsnLeu: 4.04 ± 0.074
1.099AsnMet: 1.099 ± 0.03
2.293AsnAsn: 2.293 ± 0.076
2.732AsnPro: 2.732 ± 0.069
1.313AsnGln: 1.313 ± 0.041
2.614AsnArg: 2.614 ± 0.06
2.979AsnSer: 2.979 ± 0.063
2.402AsnThr: 2.402 ± 0.056
2.93AsnVal: 2.93 ± 0.054
0.564AsnTrp: 0.564 ± 0.027
1.86AsnTyr: 1.86 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
4.097ProAla: 4.097 ± 0.081
0.365ProCys: 0.365 ± 0.021
3.277ProAsp: 3.277 ± 0.07
3.506ProGlu: 3.506 ± 0.061
1.766ProPhe: 1.766 ± 0.041
2.934ProGly: 2.934 ± 0.063
0.722ProHis: 0.722 ± 0.03
2.107ProIle: 2.107 ± 0.049
1.916ProLys: 1.916 ± 0.053
3.553ProLeu: 3.553 ± 0.057
1.05ProMet: 1.05 ± 0.035
1.388ProAsn: 1.388 ± 0.04
0.98ProPro: 0.98 ± 0.039
1.476ProGln: 1.476 ± 0.043
1.802ProArg: 1.802 ± 0.041
2.703ProSer: 2.703 ± 0.056
2.148ProThr: 2.148 ± 0.043
3.388ProVal: 3.388 ± 0.067
0.48ProTrp: 0.48 ± 0.022
1.614ProTyr: 1.614 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
2.468GlnAla: 2.468 ± 0.058
0.278GlnCys: 0.278 ± 0.017
1.288GlnAsp: 1.288 ± 0.034
1.777GlnGlu: 1.777 ± 0.051
1.232GlnPhe: 1.232 ± 0.038
1.807GlnGly: 1.807 ± 0.044
0.512GlnHis: 0.512 ± 0.024
2.036GlnIle: 2.036 ± 0.048
1.735GlnLys: 1.735 ± 0.041
2.951GlnLeu: 2.951 ± 0.062
0.89GlnMet: 0.89 ± 0.027
1.354GlnAsn: 1.354 ± 0.041
1.295GlnPro: 1.295 ± 0.041
1.294GlnGln: 1.294 ± 0.049
1.69GlnArg: 1.69 ± 0.045
1.844GlnSer: 1.844 ± 0.048
1.758GlnThr: 1.758 ± 0.046
1.714GlnVal: 1.714 ± 0.045
0.446GlnTrp: 0.446 ± 0.022
1.075GlnTyr: 1.075 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
3.605ArgAla: 3.605 ± 0.062
0.65ArgCys: 0.65 ± 0.03
2.869ArgAsp: 2.869 ± 0.058
3.605ArgGlu: 3.605 ± 0.077
2.582ArgPhe: 2.582 ± 0.059
3.148ArgGly: 3.148 ± 0.062
1.435ArgHis: 1.435 ± 0.044
4.085ArgIle: 4.085 ± 0.064
3.285ArgLys: 3.285 ± 0.068
5.816ArgLeu: 5.816 ± 0.084
1.659ArgMet: 1.659 ± 0.044
2.755ArgAsn: 2.755 ± 0.056
2.246ArgPro: 2.246 ± 0.051
2.157ArgGln: 2.157 ± 0.048
3.838ArgArg: 3.838 ± 0.077
3.182ArgSer: 3.182 ± 0.06
2.688ArgThr: 2.688 ± 0.054
3.112ArgVal: 3.112 ± 0.063
0.751ArgTrp: 0.751 ± 0.03
2.492ArgTyr: 2.492 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.562SerAla: 5.562 ± 0.093
0.836SerCys: 0.836 ± 0.029
4.101SerAsp: 4.101 ± 0.072
3.992SerGlu: 3.992 ± 0.066
2.995SerPhe: 2.995 ± 0.062
5.245SerGly: 5.245 ± 0.088
1.324SerHis: 1.324 ± 0.04
4.226SerIle: 4.226 ± 0.069
3.135SerLys: 3.135 ± 0.056
6.429SerLeu: 6.429 ± 0.095
1.654SerMet: 1.654 ± 0.043
2.577SerAsn: 2.577 ± 0.065
2.795SerPro: 2.795 ± 0.055
2.033SerGln: 2.033 ± 0.041
3.792SerArg: 3.792 ± 0.066
4.159SerSer: 4.159 ± 0.082
3.536SerThr: 3.536 ± 0.062
4.742SerVal: 4.742 ± 0.084
0.834SerTrp: 0.834 ± 0.033
2.591SerTyr: 2.591 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
5.055ThrAla: 5.055 ± 0.077
0.529ThrCys: 0.529 ± 0.024
3.757ThrAsp: 3.757 ± 0.066
3.127ThrGlu: 3.127 ± 0.068
2.61ThrPhe: 2.61 ± 0.055
4.317ThrGly: 4.317 ± 0.077
0.956ThrHis: 0.956 ± 0.03
3.805ThrIle: 3.805 ± 0.066
2.164ThrLys: 2.164 ± 0.049
5.692ThrLeu: 5.692 ± 0.081
1.193ThrMet: 1.193 ± 0.035
2.088ThrAsn: 2.088 ± 0.056
3.309ThrPro: 3.309 ± 0.062
1.49ThrGln: 1.49 ± 0.049
2.649ThrArg: 2.649 ± 0.06
3.604ThrSer: 3.604 ± 0.072
3.07ThrThr: 3.07 ± 0.063
4.456ThrVal: 4.456 ± 0.076
0.6ThrTrp: 0.6 ± 0.027
2.153ThrTyr: 2.153 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
5.54ValAla: 5.54 ± 0.102
0.934ValCys: 0.934 ± 0.033
3.954ValAsp: 3.954 ± 0.065
3.945ValGlu: 3.945 ± 0.074
2.546ValPhe: 2.546 ± 0.056
4.093ValGly: 4.093 ± 0.073
0.993ValHis: 0.993 ± 0.031
4.616ValIle: 4.616 ± 0.081
3.78ValLys: 3.78 ± 0.074
5.315ValLeu: 5.315 ± 0.095
1.877ValMet: 1.877 ± 0.044
3.205ValAsn: 3.205 ± 0.058
2.577ValPro: 2.577 ± 0.055
1.477ValGln: 1.477 ± 0.037
3.612ValArg: 3.612 ± 0.068
4.896ValSer: 4.896 ± 0.076
4.237ValThr: 4.237 ± 0.074
4.402ValVal: 4.402 ± 0.079
0.719ValTrp: 0.719 ± 0.031
2.406ValTyr: 2.406 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.787TrpAla: 0.787 ± 0.026
0.206TrpCys: 0.206 ± 0.017
0.709TrpAsp: 0.709 ± 0.027
0.688TrpGlu: 0.688 ± 0.026
0.524TrpPhe: 0.524 ± 0.024
0.912TrpGly: 0.912 ± 0.037
0.272TrpHis: 0.272 ± 0.019
0.753TrpIle: 0.753 ± 0.029
0.714TrpLys: 0.714 ± 0.03
1.212TrpLeu: 1.212 ± 0.036
0.382TrpMet: 0.382 ± 0.018
0.66TrpAsn: 0.66 ± 0.027
0.294TrpPro: 0.294 ± 0.019
0.508TrpGln: 0.508 ± 0.025
0.702TrpArg: 0.702 ± 0.025
0.731TrpSer: 0.731 ± 0.03
0.736TrpThr: 0.736 ± 0.033
0.656TrpVal: 0.656 ± 0.026
0.206TrpTrp: 0.206 ± 0.016
0.446TrpTyr: 0.446 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.058
0.564TyrCys: 0.564 ± 0.025
2.429TyrAsp: 2.429 ± 0.053
2.042TyrGlu: 2.042 ± 0.045
1.861TyrPhe: 1.861 ± 0.046
2.786TyrGly: 2.786 ± 0.057
0.785TyrHis: 0.785 ± 0.027
2.582TyrIle: 2.582 ± 0.056
1.899TyrLys: 1.899 ± 0.052
3.609TyrLeu: 3.609 ± 0.071
0.957TyrMet: 0.957 ± 0.033
2.214TyrAsn: 2.214 ± 0.059
1.735TyrPro: 1.735 ± 0.045
1.122TyrGln: 1.122 ± 0.04
2.376TyrArg: 2.376 ± 0.05
2.7TyrSer: 2.7 ± 0.059
2.309TyrThr: 2.309 ± 0.051
2.25TyrVal: 2.25 ± 0.045
0.481TyrTrp: 0.481 ± 0.021
1.727TyrTyr: 1.727 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2703 proteins (952895 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski