Camellia chlorotic dwarf-associated virus
Average proteome isoelectric point is 7.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1JHH9|A0A2P1JHH9_9GEMI Replication-associated protein OS=Camellia chlorotic dwarf-associated virus OX=2122733 PE=3 SV=1
MM1 pKa = 7.84 APTQKK6 pKa = 10.01 QFRR9 pKa = 11.84 FASKK13 pKa = 10.83 NIFLTFAQCNLLMATLLDD31 pKa = 3.95 HH32 pKa = 7.34 LKK34 pKa = 10.65 HH35 pKa = 6.2 LLSNLDD41 pKa = 3.29 IIYY44 pKa = 10.2 ISVCSEE50 pKa = 3.34 LHH52 pKa = 6.63 ASGDD56 pKa = 3.78 PHH58 pKa = 7.9 LHH60 pKa = 7.01 AMVQLKK66 pKa = 10.4 KK67 pKa = 10.19 PLQTRR72 pKa = 11.84 NPRR75 pKa = 11.84 FFDD78 pKa = 3.71 YY79 pKa = 11.27 NSDD82 pKa = 3.55 RR83 pKa = 11.84 NNFHH87 pKa = 7.37 PSFEE91 pKa = 4.17 PLRR94 pKa = 11.84 NPRR97 pKa = 11.84 ASKK100 pKa = 10.69 SYY102 pKa = 9.6 IQKK105 pKa = 10.14 DD106 pKa = 3.67 GNFIEE111 pKa = 4.83 EE112 pKa = 5.47 GEE114 pKa = 4.2 FQSNGKK120 pKa = 9.47 SPHH123 pKa = 5.92 KK124 pKa = 9.5 PANKK128 pKa = 8.39 VWQEE132 pKa = 3.8 ILQKK136 pKa = 10.07 STDD139 pKa = 3.33 EE140 pKa = 3.92 NDD142 pKa = 3.29 FYY144 pKa = 11.58 KK145 pKa = 9.79 QVKK148 pKa = 5.61 MHH150 pKa = 6.77 RR151 pKa = 11.84 PADD154 pKa = 3.89 FVLRR158 pKa = 11.84 WPAISSFAKK167 pKa = 10.13 DD168 pKa = 3.04 HH169 pKa = 5.91 YY170 pKa = 10.67 NRR172 pKa = 11.84 NFIPYY177 pKa = 8.37 TPQFEE182 pKa = 4.64 NFVNLPDD189 pKa = 5.76 NIRR192 pKa = 11.84 EE193 pKa = 4.03 WANNNVLCVSKK204 pKa = 9.69 TFVQYY209 pKa = 10.57 DD210 pKa = 3.69 LCYY213 pKa = 9.29 EE214 pKa = 4.2 CSKK217 pKa = 11.05 KK218 pKa = 10.97 AMFEE222 pKa = 4.28 TEE224 pKa = 4.28 CPISLLHH231 pKa = 7.05 NFYY234 pKa = 10.81 CDD236 pKa = 3.21 NCRR239 pKa = 11.84 SPSLDD244 pKa = 4.09 LIVHH248 pKa = 6.22 EE249 pKa = 5.38 ASTSVDD255 pKa = 3.04 QAEE258 pKa = 4.49 QEE260 pKa = 4.26 RR261 pKa = 11.84 HH262 pKa = 5.87 PGQDD266 pKa = 2.99 PSDD269 pKa = 4.21 YY270 pKa = 10.49 IIISQTPP277 pKa = 2.89
Molecular weight: 32.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.051
IPC2_protein 6.02
IPC_protein 6.122
Toseland 6.453
ProMoST 6.561
Dawson 6.478
Bjellqvist 6.453
Wikipedia 6.504
Rodwell 6.478
Grimsley 6.59
Solomon 6.478
Lehninger 6.478
Nozaki 6.766
DTASelect 6.942
Thurlkill 6.971
EMBOSS 6.942
Sillero 6.868
Patrickios 3.961
IPC_peptide 6.491
IPC2_peptide 6.795
IPC2.peptide.svr19 6.745
Protein with the highest isoelectric point:
>tr|A0A2P1JHH5|A0A2P1JHH5_9GEMI Uncharacterized protein OS=Camellia chlorotic dwarf-associated virus OX=2122733 PE=4 SV=1
MM1 pKa = 7.29 ATTRR5 pKa = 11.84 SGRR8 pKa = 11.84 VSGTVTDD15 pKa = 4.9 RR16 pKa = 11.84 LPMWRR21 pKa = 11.84 SRR23 pKa = 11.84 RR24 pKa = 11.84 KK25 pKa = 9.15 RR26 pKa = 11.84 SARR29 pKa = 11.84 SRR31 pKa = 11.84 GSATSAARR39 pKa = 11.84 RR40 pKa = 11.84 SRR42 pKa = 11.84 SAGVSIRR49 pKa = 11.84 RR50 pKa = 11.84 TGWKK54 pKa = 9.97 GKK56 pKa = 10.55 VPDD59 pKa = 4.31 GCKK62 pKa = 10.42 GPCKK66 pKa = 9.24 THH68 pKa = 6.71 SVDD71 pKa = 4.78 LIKK74 pKa = 9.83 TVYY77 pKa = 10.19 HH78 pKa = 7.28 DD79 pKa = 3.5 GRR81 pKa = 11.84 GAGFIGNIDD90 pKa = 3.5 KK91 pKa = 11.55 GEE93 pKa = 4.22 DD94 pKa = 2.9 LGNRR98 pKa = 11.84 DD99 pKa = 2.97 GRR101 pKa = 11.84 RR102 pKa = 11.84 IRR104 pKa = 11.84 ITKK107 pKa = 9.35 VLLRR111 pKa = 11.84 GKK113 pKa = 9.79 VWLDD117 pKa = 3.12 VTNAALPGSNVCKK130 pKa = 10.37 VWVIKK135 pKa = 10.1 DD136 pKa = 3.54 RR137 pKa = 11.84 RR138 pKa = 11.84 PGNEE142 pKa = 3.14 IVNFTSLMDD151 pKa = 3.74 MTDD154 pKa = 4.11 SEE156 pKa = 4.67 PMSALIKK163 pKa = 9.58 MDD165 pKa = 3.17 YY166 pKa = 9.9 RR167 pKa = 11.84 DD168 pKa = 3.57 RR169 pKa = 11.84 FIVLRR174 pKa = 11.84 DD175 pKa = 3.52 MQFDD179 pKa = 3.48 LHH181 pKa = 6.62 GGKK184 pKa = 10.13 DD185 pKa = 3.6 FRR187 pKa = 11.84 VDD189 pKa = 3.41 EE190 pKa = 4.52 ASMDD194 pKa = 3.41 EE195 pKa = 4.43 MISVNCEE202 pKa = 3.72 SLFSHH207 pKa = 6.7 EE208 pKa = 5.12 DD209 pKa = 3.22 EE210 pKa = 5.63 GNLSHH215 pKa = 7.05 CLEE218 pKa = 4.43 NGILVYY224 pKa = 8.39 FACSDD229 pKa = 3.72 PRR231 pKa = 11.84 NAMQITAQCRR241 pKa = 11.84 VYY243 pKa = 10.97 FFDD246 pKa = 3.69 STVNN250 pKa = 3.3
Molecular weight: 28.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.115
IPC2_protein 8.726
IPC_protein 8.712
Toseland 9.502
ProMoST 9.253
Dawson 9.736
Bjellqvist 9.545
Wikipedia 9.867
Rodwell 10.014
Grimsley 9.75
Solomon 9.809
Lehninger 9.78
Nozaki 9.809
DTASelect 9.443
Thurlkill 9.633
EMBOSS 9.94
Sillero 9.765
Patrickios 7.629
IPC_peptide 9.809
IPC2_peptide 8.492
IPC2.peptide.svr19 7.746
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1408
108
333
234.7
26.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.185 ± 0.412
2.983 ± 0.723
5.895 ± 0.636
5.398 ± 0.828
4.972 ± 0.602
5.611 ± 1.365
2.699 ± 0.601
5.398 ± 0.218
6.392 ± 0.412
7.528 ± 0.389
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.912 ± 0.343
5.824 ± 1.011
5.611 ± 0.678
3.196 ± 0.726
6.179 ± 1.02
8.594 ± 0.848
3.693 ± 0.701
6.676 ± 0.993
1.705 ± 0.252
3.551 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here