Amino acid dipepetide frequency for Pseudomonas phage phi-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.862AlaAla: 15.862 ± 1.899
1.089AlaCys: 1.089 ± 0.343
8.631AlaAsp: 8.631 ± 0.907
6.842AlaGlu: 6.842 ± 0.633
2.41AlaPhe: 2.41 ± 0.471
8.942AlaGly: 8.942 ± 1.199
1.555AlaHis: 1.555 ± 0.372
3.343AlaIle: 3.343 ± 0.424
6.376AlaLys: 6.376 ± 0.602
10.264AlaLeu: 10.264 ± 1.042
2.955AlaMet: 2.955 ± 0.649
3.732AlaAsn: 3.732 ± 0.753
5.521AlaPro: 5.521 ± 1.329
6.22AlaGln: 6.22 ± 0.868
6.376AlaArg: 6.376 ± 0.583
5.365AlaSer: 5.365 ± 0.782
6.609AlaThr: 6.609 ± 0.847
8.164AlaVal: 8.164 ± 0.829
1.166AlaTrp: 1.166 ± 0.293
2.877AlaTyr: 2.877 ± 0.535
0.0AlaXaa: 0.0 ± 0.0
Cys
0.7CysAla: 0.7 ± 0.263
0.0CysCys: 0.0 ± 0.0
0.622CysAsp: 0.622 ± 0.222
0.544CysGlu: 0.544 ± 0.272
0.156CysPhe: 0.156 ± 0.102
1.089CysGly: 1.089 ± 0.398
0.622CysHis: 0.622 ± 0.247
0.622CysIle: 0.622 ± 0.189
0.311CysLys: 0.311 ± 0.171
1.089CysLeu: 1.089 ± 0.369
0.389CysMet: 0.389 ± 0.261
0.7CysAsn: 0.7 ± 0.26
1.244CysPro: 1.244 ± 0.392
0.389CysGln: 0.389 ± 0.205
0.778CysArg: 0.778 ± 0.299
0.544CysSer: 0.544 ± 0.212
0.778CysThr: 0.778 ± 0.194
0.467CysVal: 0.467 ± 0.18
0.156CysTrp: 0.156 ± 0.135
0.389CysTyr: 0.389 ± 0.239
0.0CysXaa: 0.0 ± 0.0
Asp
6.92AspAla: 6.92 ± 0.857
0.622AspCys: 0.622 ± 0.223
3.577AspAsp: 3.577 ± 0.642
3.732AspGlu: 3.732 ± 0.486
2.644AspPhe: 2.644 ± 0.472
5.287AspGly: 5.287 ± 0.763
1.089AspHis: 1.089 ± 0.291
2.877AspIle: 2.877 ± 0.521
3.499AspLys: 3.499 ± 0.622
6.298AspLeu: 6.298 ± 0.715
1.788AspMet: 1.788 ± 0.342
2.177AspAsn: 2.177 ± 0.331
2.41AspPro: 2.41 ± 0.459
2.255AspGln: 2.255 ± 0.391
3.421AspArg: 3.421 ± 0.468
2.566AspSer: 2.566 ± 0.45
3.81AspThr: 3.81 ± 0.428
4.276AspVal: 4.276 ± 0.6
0.7AspTrp: 0.7 ± 0.217
1.788AspTyr: 1.788 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
8.009GluAla: 8.009 ± 0.604
0.622GluCys: 0.622 ± 0.207
3.343GluAsp: 3.343 ± 0.533
2.41GluGlu: 2.41 ± 0.501
2.022GluPhe: 2.022 ± 0.374
3.888GluGly: 3.888 ± 0.567
1.244GluHis: 1.244 ± 0.358
1.477GluIle: 1.477 ± 0.349
2.799GluLys: 2.799 ± 0.491
4.432GluLeu: 4.432 ± 0.522
1.944GluMet: 1.944 ± 0.482
1.322GluAsn: 1.322 ± 0.231
2.099GluPro: 2.099 ± 0.474
3.343GluGln: 3.343 ± 0.612
4.043GluArg: 4.043 ± 0.742
2.721GluSer: 2.721 ± 0.484
2.721GluThr: 2.721 ± 0.346
4.432GluVal: 4.432 ± 0.655
1.011GluTrp: 1.011 ± 0.343
2.644GluTyr: 2.644 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
3.421PheAla: 3.421 ± 0.386
0.7PheCys: 0.7 ± 0.196
2.799PheAsp: 2.799 ± 0.497
1.477PheGlu: 1.477 ± 0.313
1.166PhePhe: 1.166 ± 0.313
2.799PheGly: 2.799 ± 0.373
0.855PheHis: 0.855 ± 0.199
1.244PheIle: 1.244 ± 0.248
1.866PheLys: 1.866 ± 0.436
2.488PheLeu: 2.488 ± 0.462
1.011PheMet: 1.011 ± 0.237
1.477PheAsn: 1.477 ± 0.349
1.555PhePro: 1.555 ± 0.367
1.555PheGln: 1.555 ± 0.397
1.711PheArg: 1.711 ± 0.345
1.244PheSer: 1.244 ± 0.319
2.255PheThr: 2.255 ± 0.46
2.177PheVal: 2.177 ± 0.416
0.233PheTrp: 0.233 ± 0.134
0.389PheTyr: 0.389 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
6.609GlyAla: 6.609 ± 0.994
0.622GlyCys: 0.622 ± 0.198
4.899GlyAsp: 4.899 ± 0.516
3.965GlyGlu: 3.965 ± 0.566
2.799GlyPhe: 2.799 ± 0.577
6.22GlyGly: 6.22 ± 0.776
1.244GlyHis: 1.244 ± 0.294
3.188GlyIle: 3.188 ± 0.579
5.521GlyLys: 5.521 ± 0.781
6.531GlyLeu: 6.531 ± 0.793
1.866GlyMet: 1.866 ± 0.308
3.11GlyAsn: 3.11 ± 0.783
2.255GlyPro: 2.255 ± 0.379
3.343GlyGln: 3.343 ± 0.583
4.51GlyArg: 4.51 ± 0.477
3.032GlySer: 3.032 ± 0.324
5.909GlyThr: 5.909 ± 0.733
7.153GlyVal: 7.153 ± 0.568
1.477GlyTrp: 1.477 ± 0.386
2.721GlyTyr: 2.721 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
2.099HisAla: 2.099 ± 0.414
0.467HisCys: 0.467 ± 0.212
1.477HisAsp: 1.477 ± 0.399
1.089HisGlu: 1.089 ± 0.295
0.311HisPhe: 0.311 ± 0.172
2.099HisGly: 2.099 ± 0.435
0.7HisHis: 0.7 ± 0.297
0.778HisIle: 0.778 ± 0.265
1.244HisLys: 1.244 ± 0.253
1.866HisLeu: 1.866 ± 0.483
0.389HisMet: 0.389 ± 0.161
0.855HisAsn: 0.855 ± 0.198
1.011HisPro: 1.011 ± 0.335
0.933HisGln: 0.933 ± 0.231
2.177HisArg: 2.177 ± 0.521
0.855HisSer: 0.855 ± 0.218
0.933HisThr: 0.933 ± 0.313
1.633HisVal: 1.633 ± 0.385
0.7HisTrp: 0.7 ± 0.222
0.7HisTyr: 0.7 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
4.199IleAla: 4.199 ± 0.535
0.467IleCys: 0.467 ± 0.23
2.955IleAsp: 2.955 ± 0.378
3.032IleGlu: 3.032 ± 0.349
1.166IlePhe: 1.166 ± 0.245
2.566IleGly: 2.566 ± 0.39
0.622IleHis: 0.622 ± 0.142
1.866IleIle: 1.866 ± 0.434
2.799IleLys: 2.799 ± 0.49
2.721IleLeu: 2.721 ± 0.476
1.011IleMet: 1.011 ± 0.275
1.788IleAsn: 1.788 ± 0.357
2.022IlePro: 2.022 ± 0.337
1.477IleGln: 1.477 ± 0.287
2.566IleArg: 2.566 ± 0.521
1.477IleSer: 1.477 ± 0.304
2.877IleThr: 2.877 ± 0.642
2.488IleVal: 2.488 ± 0.457
0.7IleTrp: 0.7 ± 0.273
0.855IleTyr: 0.855 ± 0.225
0.0IleXaa: 0.0 ± 0.0
Lys
6.531LysAla: 6.531 ± 0.978
0.544LysCys: 0.544 ± 0.24
3.11LysAsp: 3.11 ± 0.525
3.499LysGlu: 3.499 ± 0.564
1.944LysPhe: 1.944 ± 0.352
4.51LysGly: 4.51 ± 0.685
1.4LysHis: 1.4 ± 0.339
1.244LysIle: 1.244 ± 0.294
2.566LysLys: 2.566 ± 0.789
5.598LysLeu: 5.598 ± 0.703
1.166LysMet: 1.166 ± 0.357
1.555LysAsn: 1.555 ± 0.276
3.032LysPro: 3.032 ± 0.587
2.566LysGln: 2.566 ± 0.484
2.799LysArg: 2.799 ± 0.631
2.41LysSer: 2.41 ± 0.354
2.721LysThr: 2.721 ± 0.565
3.654LysVal: 3.654 ± 0.558
0.933LysTrp: 0.933 ± 0.237
1.011LysTyr: 1.011 ± 0.24
0.0LysXaa: 0.0 ± 0.0
Leu
9.875LeuAla: 9.875 ± 0.63
1.477LeuCys: 1.477 ± 0.398
5.521LeuAsp: 5.521 ± 0.849
4.121LeuGlu: 4.121 ± 0.537
2.721LeuPhe: 2.721 ± 0.487
7.464LeuGly: 7.464 ± 0.828
1.711LeuHis: 1.711 ± 0.333
3.032LeuIle: 3.032 ± 0.504
4.043LeuLys: 4.043 ± 0.712
7.231LeuLeu: 7.231 ± 0.888
2.41LeuMet: 2.41 ± 0.345
3.654LeuAsn: 3.654 ± 0.606
5.132LeuPro: 5.132 ± 0.713
3.11LeuGln: 3.11 ± 0.561
5.443LeuArg: 5.443 ± 0.675
5.832LeuSer: 5.832 ± 0.587
5.21LeuThr: 5.21 ± 0.681
5.521LeuVal: 5.521 ± 0.767
1.555LeuTrp: 1.555 ± 0.394
2.177LeuTyr: 2.177 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
3.421MetAla: 3.421 ± 0.593
0.156MetCys: 0.156 ± 0.114
1.555MetAsp: 1.555 ± 0.358
1.4MetGlu: 1.4 ± 0.338
0.778MetPhe: 0.778 ± 0.312
1.089MetGly: 1.089 ± 0.225
0.855MetHis: 0.855 ± 0.309
0.933MetIle: 0.933 ± 0.224
1.089MetLys: 1.089 ± 0.243
2.022MetLeu: 2.022 ± 0.38
1.011MetMet: 1.011 ± 0.226
1.166MetAsn: 1.166 ± 0.292
1.166MetPro: 1.166 ± 0.233
1.166MetGln: 1.166 ± 0.363
1.866MetArg: 1.866 ± 0.32
2.566MetSer: 2.566 ± 0.484
2.022MetThr: 2.022 ± 0.35
1.633MetVal: 1.633 ± 0.269
0.467MetTrp: 0.467 ± 0.17
0.933MetTyr: 0.933 ± 0.325
0.0MetXaa: 0.0 ± 0.0
Asn
4.743AsnAla: 4.743 ± 0.86
0.467AsnCys: 0.467 ± 0.221
1.788AsnAsp: 1.788 ± 0.358
2.333AsnGlu: 2.333 ± 0.407
0.933AsnPhe: 0.933 ± 0.294
2.799AsnGly: 2.799 ± 0.518
0.467AsnHis: 0.467 ± 0.206
2.099AsnIle: 2.099 ± 0.395
2.255AsnLys: 2.255 ± 0.32
2.877AsnLeu: 2.877 ± 0.395
0.933AsnMet: 0.933 ± 0.264
2.099AsnAsn: 2.099 ± 0.341
1.788AsnPro: 1.788 ± 0.311
1.555AsnGln: 1.555 ± 0.349
1.711AsnArg: 1.711 ± 0.324
2.644AsnSer: 2.644 ± 0.449
2.566AsnThr: 2.566 ± 0.518
3.266AsnVal: 3.266 ± 0.445
0.544AsnTrp: 0.544 ± 0.199
0.855AsnTyr: 0.855 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
6.143ProAla: 6.143 ± 1.296
0.467ProCys: 0.467 ± 0.214
2.799ProAsp: 2.799 ± 0.458
4.043ProGlu: 4.043 ± 0.589
1.322ProPhe: 1.322 ± 0.341
3.888ProGly: 3.888 ± 0.557
0.622ProHis: 0.622 ± 0.227
1.4ProIle: 1.4 ± 0.37
2.099ProLys: 2.099 ± 0.379
3.499ProLeu: 3.499 ± 0.545
1.166ProMet: 1.166 ± 0.298
1.244ProAsn: 1.244 ± 0.236
1.711ProPro: 1.711 ± 0.454
1.089ProGln: 1.089 ± 0.227
2.488ProArg: 2.488 ± 0.524
2.333ProSer: 2.333 ± 0.555
2.488ProThr: 2.488 ± 0.396
3.732ProVal: 3.732 ± 0.63
0.7ProTrp: 0.7 ± 0.196
1.633ProTyr: 1.633 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
5.754GlnAla: 5.754 ± 0.874
0.311GlnCys: 0.311 ± 0.167
1.633GlnAsp: 1.633 ± 0.467
2.333GlnGlu: 2.333 ± 0.442
1.711GlnPhe: 1.711 ± 0.333
3.11GlnGly: 3.11 ± 0.465
1.089GlnHis: 1.089 ± 0.246
1.477GlnIle: 1.477 ± 0.272
1.866GlnLys: 1.866 ± 0.324
4.276GlnLeu: 4.276 ± 0.606
1.477GlnMet: 1.477 ± 0.494
1.866GlnAsn: 1.866 ± 0.421
1.555GlnPro: 1.555 ± 0.388
1.788GlnGln: 1.788 ± 0.356
2.955GlnArg: 2.955 ± 0.579
1.944GlnSer: 1.944 ± 0.405
1.866GlnThr: 1.866 ± 0.428
2.333GlnVal: 2.333 ± 0.582
1.011GlnTrp: 1.011 ± 0.33
2.177GlnTyr: 2.177 ± 0.392
0.0GlnXaa: 0.0 ± 0.0
Arg
5.676ArgAla: 5.676 ± 0.593
0.855ArgCys: 0.855 ± 0.242
3.421ArgAsp: 3.421 ± 0.414
3.343ArgGlu: 3.343 ± 0.644
2.333ArgPhe: 2.333 ± 0.445
4.199ArgGly: 4.199 ± 0.578
2.255ArgHis: 2.255 ± 0.379
3.499ArgIle: 3.499 ± 0.531
2.955ArgLys: 2.955 ± 0.556
4.976ArgLeu: 4.976 ± 0.631
2.566ArgMet: 2.566 ± 0.344
1.788ArgAsn: 1.788 ± 0.436
1.944ArgPro: 1.944 ± 0.399
1.866ArgGln: 1.866 ± 0.285
4.588ArgArg: 4.588 ± 0.671
3.81ArgSer: 3.81 ± 0.81
3.188ArgThr: 3.188 ± 0.536
4.821ArgVal: 4.821 ± 0.621
1.322ArgTrp: 1.322 ± 0.321
1.944ArgTyr: 1.944 ± 0.438
0.0ArgXaa: 0.0 ± 0.0
Ser
4.51SerAla: 4.51 ± 0.611
0.467SerCys: 0.467 ± 0.229
2.721SerAsp: 2.721 ± 0.448
2.955SerGlu: 2.955 ± 0.423
2.255SerPhe: 2.255 ± 0.551
4.354SerGly: 4.354 ± 0.735
1.244SerHis: 1.244 ± 0.3
3.343SerIle: 3.343 ± 0.482
2.955SerLys: 2.955 ± 0.527
4.743SerLeu: 4.743 ± 0.502
1.244SerMet: 1.244 ± 0.355
2.099SerAsn: 2.099 ± 0.584
1.477SerPro: 1.477 ± 0.302
1.944SerGln: 1.944 ± 0.418
3.965SerArg: 3.965 ± 0.562
2.955SerSer: 2.955 ± 0.651
3.266SerThr: 3.266 ± 0.592
3.888SerVal: 3.888 ± 0.678
0.7SerTrp: 0.7 ± 0.221
1.322SerTyr: 1.322 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
7.542ThrAla: 7.542 ± 0.966
0.778ThrCys: 0.778 ± 0.253
3.965ThrAsp: 3.965 ± 0.544
2.721ThrGlu: 2.721 ± 0.42
2.177ThrPhe: 2.177 ± 0.339
4.976ThrGly: 4.976 ± 0.664
1.4ThrHis: 1.4 ± 0.328
2.644ThrIle: 2.644 ± 0.492
3.266ThrLys: 3.266 ± 0.504
4.821ThrLeu: 4.821 ± 0.61
1.244ThrMet: 1.244 ± 0.378
2.721ThrAsn: 2.721 ± 0.575
3.577ThrPro: 3.577 ± 0.505
2.488ThrGln: 2.488 ± 0.351
2.022ThrArg: 2.022 ± 0.45
3.266ThrSer: 3.266 ± 0.469
3.654ThrThr: 3.654 ± 0.734
4.743ThrVal: 4.743 ± 0.839
0.622ThrTrp: 0.622 ± 0.194
1.555ThrTyr: 1.555 ± 0.29
0.0ThrXaa: 0.0 ± 0.0
Val
8.397ValAla: 8.397 ± 1.043
0.778ValCys: 0.778 ± 0.309
4.976ValAsp: 4.976 ± 0.559
4.199ValGlu: 4.199 ± 0.535
1.788ValPhe: 1.788 ± 0.422
5.054ValGly: 5.054 ± 0.65
2.488ValHis: 2.488 ± 0.472
2.644ValIle: 2.644 ± 0.521
3.032ValLys: 3.032 ± 0.612
6.92ValLeu: 6.92 ± 0.722
1.711ValMet: 1.711 ± 0.281
2.644ValAsn: 2.644 ± 0.488
3.654ValPro: 3.654 ± 0.75
2.721ValGln: 2.721 ± 0.506
4.976ValArg: 4.976 ± 0.569
4.354ValSer: 4.354 ± 0.612
4.821ValThr: 4.821 ± 0.964
4.354ValVal: 4.354 ± 0.52
1.166ValTrp: 1.166 ± 0.334
1.711ValTyr: 1.711 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
1.711TrpAla: 1.711 ± 0.357
0.467TrpCys: 0.467 ± 0.177
1.011TrpAsp: 1.011 ± 0.242
0.778TrpGlu: 0.778 ± 0.223
0.544TrpPhe: 0.544 ± 0.267
0.467TrpGly: 0.467 ± 0.163
0.389TrpHis: 0.389 ± 0.207
0.389TrpIle: 0.389 ± 0.159
1.089TrpLys: 1.089 ± 0.272
1.944TrpLeu: 1.944 ± 0.478
0.389TrpMet: 0.389 ± 0.2
0.778TrpAsn: 0.778 ± 0.208
0.544TrpPro: 0.544 ± 0.206
0.855TrpGln: 0.855 ± 0.225
0.778TrpArg: 0.778 ± 0.26
1.166TrpSer: 1.166 ± 0.256
0.544TrpThr: 0.544 ± 0.176
1.244TrpVal: 1.244 ± 0.49
0.0TrpTrp: 0.0 ± 0.0
0.544TrpTyr: 0.544 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.177TyrAla: 2.177 ± 0.392
0.233TyrCys: 0.233 ± 0.141
0.933TyrAsp: 0.933 ± 0.271
1.711TyrGlu: 1.711 ± 0.399
1.244TyrPhe: 1.244 ± 0.331
2.177TyrGly: 2.177 ± 0.383
0.467TyrHis: 0.467 ± 0.212
1.555TyrIle: 1.555 ± 0.309
1.166TyrLys: 1.166 ± 0.299
2.644TyrLeu: 2.644 ± 0.478
0.622TyrMet: 0.622 ± 0.227
1.944TyrAsn: 1.944 ± 0.42
1.166TyrPro: 1.166 ± 0.265
1.866TyrGln: 1.866 ± 0.275
2.177TyrArg: 2.177 ± 0.331
1.322TyrSer: 1.322 ± 0.295
1.944TyrThr: 1.944 ± 0.347
2.41TyrVal: 2.41 ± 0.449
0.467TyrTrp: 0.467 ± 0.196
0.855TyrTyr: 0.855 ± 0.288
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (12862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski