Bacteroides sp. CAG:754
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4054 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6DIU5|R6DIU5_9BACE TPR_REGION domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_00208 PE=4 SV=1
MM1 pKa = 7.67 WKK3 pKa = 10.32 FKK5 pKa = 10.37 FGAWAVVLMMTALSFSACSDD25 pKa = 3.96 DD26 pKa = 5.9 DD27 pKa = 5.11 DD28 pKa = 6.57 DD29 pKa = 5.02 PALVPPSNITEE40 pKa = 4.2 ALKK43 pKa = 10.48 QLYY46 pKa = 8.51 PAAQNIEE53 pKa = 3.84 WEE55 pKa = 4.47 MKK57 pKa = 9.48 GDD59 pKa = 4.09 YY60 pKa = 10.28 YY61 pKa = 11.67 VADD64 pKa = 3.66 CWVTGDD70 pKa = 4.82 EE71 pKa = 4.31 LDD73 pKa = 3.44 VWFDD77 pKa = 4.11 ANANWVMTEE86 pKa = 4.01 NEE88 pKa = 4.68 LNSIDD93 pKa = 3.83 QLVPAVYY100 pKa = 8.52 TAFQNSDD107 pKa = 2.85 YY108 pKa = 10.49 SAWVVTDD115 pKa = 3.8 VYY117 pKa = 11.62 VLTYY121 pKa = 7.88 PQNMTEE127 pKa = 4.63 SVIQVKK133 pKa = 10.34 LGSQRR138 pKa = 11.84 YY139 pKa = 8.37 SLYY142 pKa = 10.29 FSQEE146 pKa = 3.14 GGLLHH151 pKa = 7.08 KK152 pKa = 10.44 KK153 pKa = 10.31 DD154 pKa = 3.91 ISNGDD159 pKa = 3.54 DD160 pKa = 3.75 TNWPPTEE167 pKa = 4.08 NNN169 pKa = 3.11
Molecular weight: 19.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|R6DFJ4|R6DFJ4_9BACE OMP_b-brl domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_02114 PE=4 SV=1
MM1 pKa = 7.75 HH2 pKa = 7.48 IPSQTYY8 pKa = 9.15 RR9 pKa = 11.84 PRR11 pKa = 11.84 IPEE14 pKa = 3.95 TIVRR18 pKa = 11.84 SEE20 pKa = 4.01 HH21 pKa = 6.34 RR22 pKa = 11.84 CTIVTIIEE30 pKa = 4.01 IQQITVIIRR39 pKa = 11.84 ISPIDD44 pKa = 3.57 IEE46 pKa = 4.3 TGRR49 pKa = 11.84 TFLILTVFRR58 pKa = 11.84 FSAIGIAIGKK68 pKa = 8.93 GRR70 pKa = 11.84 SSKK73 pKa = 10.95 LIFRR77 pKa = 11.84 KK78 pKa = 8.04 TICRR82 pKa = 11.84 IAVHH86 pKa = 6.83 CILITTEE93 pKa = 3.07 IKK95 pKa = 10.15 ARR97 pKa = 11.84 YY98 pKa = 8.27 GIHH101 pKa = 6.88 HH102 pKa = 6.67 MFVCEE107 pKa = 4.01 KK108 pKa = 8.25 FTKK111 pKa = 10.13 RR112 pKa = 11.84 RR113 pKa = 11.84 GKK115 pKa = 10.08 IPFIYY120 pKa = 8.43 HH121 pKa = 5.65 TLCQRR126 pKa = 11.84 MSKK129 pKa = 10.27 QSFLIFSHH137 pKa = 6.98 IIKK140 pKa = 10.52 LL141 pKa = 3.73
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.721
IPC_protein 10.452
Toseland 10.818
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.155
Grimsley 10.935
Solomon 11.023
Lehninger 10.994
Nozaki 10.818
DTASelect 10.584
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.833
Patrickios 10.906
IPC_peptide 11.023
IPC2_peptide 9.809
IPC2.peptide.svr19 8.536
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4054
0
4054
1597693
29
1986
394.1
44.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.849 ± 0.036
1.192 ± 0.014
5.557 ± 0.023
6.491 ± 0.034
4.584 ± 0.026
6.964 ± 0.031
1.776 ± 0.014
6.759 ± 0.035
6.619 ± 0.028
8.925 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.016
5.363 ± 0.035
3.741 ± 0.02
3.441 ± 0.019
4.476 ± 0.023
6.287 ± 0.029
5.789 ± 0.027
6.371 ± 0.029
1.392 ± 0.014
4.713 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here