Bacteroides sp. CAG:754 
Average proteome isoelectric point is 6.34 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 4054 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|R6DIU5|R6DIU5_9BACE TPR_REGION domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_00208 PE=4 SV=1 
MM1 pKa = 7.67  WKK3 pKa = 10.32  FKK5 pKa = 10.37  FGAWAVVLMMTALSFSACSDD25 pKa = 3.96  DD26 pKa = 5.9  DD27 pKa = 5.11  DD28 pKa = 6.57  DD29 pKa = 5.02  PALVPPSNITEE40 pKa = 4.2  ALKK43 pKa = 10.48  QLYY46 pKa = 8.51  PAAQNIEE53 pKa = 3.84  WEE55 pKa = 4.47  MKK57 pKa = 9.48  GDD59 pKa = 4.09  YY60 pKa = 10.28  YY61 pKa = 11.67  VADD64 pKa = 3.66  CWVTGDD70 pKa = 4.82  EE71 pKa = 4.31  LDD73 pKa = 3.44  VWFDD77 pKa = 4.11  ANANWVMTEE86 pKa = 4.01  NEE88 pKa = 4.68  LNSIDD93 pKa = 3.83  QLVPAVYY100 pKa = 8.52  TAFQNSDD107 pKa = 2.85  YY108 pKa = 10.49  SAWVVTDD115 pKa = 3.8  VYY117 pKa = 11.62  VLTYY121 pKa = 7.88  PQNMTEE127 pKa = 4.63  SVIQVKK133 pKa = 10.34  LGSQRR138 pKa = 11.84  YY139 pKa = 8.37  SLYY142 pKa = 10.29  FSQEE146 pKa = 3.14  GGLLHH151 pKa = 7.08  KK152 pKa = 10.44  KK153 pKa = 10.31  DD154 pKa = 3.91  ISNGDD159 pKa = 3.54  DD160 pKa = 3.75  TNWPPTEE167 pKa = 4.08  NNN169 pKa = 3.11   
 Molecular weight: 19.19 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.739 
IPC2_protein 3.808 
IPC_protein 3.795 
Toseland    3.579 
ProMoST     3.973 
Dawson      3.795 
Bjellqvist  3.948 
Wikipedia   3.745 
Rodwell     3.63 
Grimsley    3.49 
Solomon     3.783 
Lehninger   3.745 
Nozaki      3.91 
DTASelect   4.151 
Thurlkill   3.643 
EMBOSS      3.745 
Sillero     3.923 
Patrickios  0.693 
IPC_peptide 3.783 
IPC2_peptide  3.897 
IPC2.peptide.svr19  3.817 
 Protein with the highest isoelectric point: 
>tr|R6DFJ4|R6DFJ4_9BACE OMP_b-brl domain-containing protein OS=Bacteroides sp. CAG:754 OX=1262750 GN=BN772_02114 PE=4 SV=1 
MM1 pKa = 7.75  HH2 pKa = 7.48  IPSQTYY8 pKa = 9.15  RR9 pKa = 11.84  PRR11 pKa = 11.84  IPEE14 pKa = 3.95  TIVRR18 pKa = 11.84  SEE20 pKa = 4.01  HH21 pKa = 6.34  RR22 pKa = 11.84  CTIVTIIEE30 pKa = 4.01  IQQITVIIRR39 pKa = 11.84  ISPIDD44 pKa = 3.57  IEE46 pKa = 4.3  TGRR49 pKa = 11.84  TFLILTVFRR58 pKa = 11.84  FSAIGIAIGKK68 pKa = 8.93  GRR70 pKa = 11.84  SSKK73 pKa = 10.95  LIFRR77 pKa = 11.84  KK78 pKa = 8.04  TICRR82 pKa = 11.84  IAVHH86 pKa = 6.83  CILITTEE93 pKa = 3.07  IKK95 pKa = 10.15  ARR97 pKa = 11.84  YY98 pKa = 8.27  GIHH101 pKa = 6.88  HH102 pKa = 6.67  MFVCEE107 pKa = 4.01  KK108 pKa = 8.25  FTKK111 pKa = 10.13  RR112 pKa = 11.84  RR113 pKa = 11.84  GKK115 pKa = 10.08  IPFIYY120 pKa = 8.43  HH121 pKa = 5.65  TLCQRR126 pKa = 11.84  MSKK129 pKa = 10.27  QSFLIFSHH137 pKa = 6.98  IIKK140 pKa = 10.52  LL141 pKa = 3.73   
 Molecular weight: 16.45 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.345 
IPC2_protein 9.721 
IPC_protein 10.452 
Toseland    10.818 
ProMoST     10.482 
Dawson      10.891 
Bjellqvist  10.599 
Wikipedia   11.096 
Rodwell     11.155 
Grimsley    10.935 
Solomon     11.023 
Lehninger   10.994 
Nozaki      10.818 
DTASelect   10.584 
Thurlkill   10.804 
EMBOSS      11.228 
Sillero     10.833 
Patrickios  10.906 
IPC_peptide 11.023 
IPC2_peptide  9.809 
IPC2.peptide.svr19  8.536 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        4054 
 
        
        0
 
        
        4054 
         
        1597693
 
        29
 
        1986
 
        394.1
 
        44.51
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        6.849 ± 0.036
1.192 ± 0.014
 
        5.557 ± 0.023
6.491 ± 0.034
 
        4.584 ± 0.026
6.964 ± 0.031
 
        1.776 ± 0.014
6.759 ± 0.035
       
        6.619 ± 0.028
8.925 ± 0.038
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.707 ± 0.016
5.363 ± 0.035
 
        3.741 ± 0.02
3.441 ± 0.019
 
        4.476 ± 0.023
6.287 ± 0.029
 
        5.789 ± 0.027
6.371 ± 0.029
       
        1.392 ± 0.014
4.713 ± 0.025
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here