Halanaeroarchaeum sulfurireducens

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halanaeroarchaeum

Average proteome isoelectric point is 4.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7PBN9|A0A0F7PBN9_9EURY Phosphoglycerate kinase OS=Halanaeroarchaeum sulfurireducens OX=1604004 GN=pgk PE=3 SV=1
MM1 pKa = 7.42GLSEE5 pKa = 4.17TLSTFFGFEE14 pKa = 3.74EE15 pKa = 5.07HH16 pKa = 7.15DD17 pKa = 3.31TDD19 pKa = 5.44LRR21 pKa = 11.84TEE23 pKa = 3.9ILAGVTTFLTMSYY36 pKa = 9.96IVVVNPAILTDD47 pKa = 3.54QPGDD51 pKa = 3.36GGFTDD56 pKa = 6.17GISLANHH63 pKa = 6.26SPGEE67 pKa = 4.19VQQMLAVVTILAASVAIFVMAFYY90 pKa = 11.02ANRR93 pKa = 11.84PFAQAPGLGLNAFFAFTVTGAMGIPWEE120 pKa = 4.13TALAAVFVEE129 pKa = 4.05GLVFILLTSVGARR142 pKa = 11.84EE143 pKa = 4.07YY144 pKa = 11.02VISLFPAPVKK154 pKa = 10.47FSVGTGIGLFLAIIGLQAMGIVAPDD179 pKa = 3.95EE180 pKa = 4.01ATMIAMGDD188 pKa = 3.84LAQDD192 pKa = 3.86PVAVLSVVGLLFTFALYY209 pKa = 10.79ARR211 pKa = 11.84GIPGAIVFGIIGTTIAGWALTAAGVTPPGVLAPEE245 pKa = 4.35SLPGAQYY252 pKa = 10.69DD253 pKa = 3.6ISPLAGAFIEE263 pKa = 5.16GFANVEE269 pKa = 4.01AFSFALVVFTFFFVDD284 pKa = 4.8FFDD287 pKa = 3.88TAGTLTGVSQVAGFLDD303 pKa = 4.75DD304 pKa = 4.96EE305 pKa = 5.23GNLPDD310 pKa = 4.68ADD312 pKa = 4.26KK313 pKa = 11.15PLMADD318 pKa = 4.37AIGTTVGSMLGTSTVTTFVEE338 pKa = 4.47SATGVEE344 pKa = 4.02AGGRR348 pKa = 11.84TGMTAFVVGVLFLLSLAIVPLAAAIPQYY376 pKa = 10.65ASHH379 pKa = 6.93IALVVVAVIMFANVTDD395 pKa = 4.1IQWDD399 pKa = 4.06DD400 pKa = 3.57LTHH403 pKa = 7.32AIPAGLTIIVMPFTYY418 pKa = 10.37SIAYY422 pKa = 8.97GIAAGIVSYY431 pKa = 9.62PIVKK435 pKa = 10.07SAVGEE440 pKa = 3.83YY441 pKa = 10.93DD442 pKa = 4.02EE443 pKa = 5.0IHH445 pKa = 5.83VGQWVLAAAFVLYY458 pKa = 9.41FFVRR462 pKa = 11.84TGGILTGAVV471 pKa = 3.18

Molecular weight: 48.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7P769|A0A0F7P769_9EURY UspA domain-containing protein OS=Halanaeroarchaeum sulfurireducens OX=1604004 GN=uspA1 PE=4 SV=1
MM1 pKa = 7.54SRR3 pKa = 11.84RR4 pKa = 11.84TPHH7 pKa = 6.1IPEE10 pKa = 3.97RR11 pKa = 11.84VRR13 pKa = 11.84RR14 pKa = 11.84GPTSSRR20 pKa = 11.84NSRR23 pKa = 11.84RR24 pKa = 11.84PARR27 pKa = 11.84FASVSSRR34 pKa = 11.84FRR36 pKa = 11.84PTTSTTPGPTKK47 pKa = 9.53RR48 pKa = 11.84HH49 pKa = 5.16SRR51 pKa = 11.84PTHH54 pKa = 5.42RR55 pKa = 11.84QNGSPVTNRR64 pKa = 11.84WPGAPASEE72 pKa = 4.46RR73 pKa = 11.84GPKK76 pKa = 8.12VTARR80 pKa = 11.84RR81 pKa = 11.84TAVSTPSRR89 pKa = 11.84RR90 pKa = 11.84FSRR93 pKa = 11.84TTRR96 pKa = 11.84RR97 pKa = 11.84SVRR100 pKa = 11.84FGTVLAPRR108 pKa = 11.84RR109 pKa = 11.84NGGPRR114 pKa = 11.84SGGSTTT120 pKa = 3.63

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2225

0

2225

637001

34

1872

286.3

31.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.45 ± 0.077

0.549 ± 0.017

8.407 ± 0.069

8.751 ± 0.079

3.242 ± 0.031

8.118 ± 0.049

2.058 ± 0.029

4.57 ± 0.042

2.066 ± 0.034

8.588 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.894 ± 0.02

2.416 ± 0.032

4.63 ± 0.038

2.593 ± 0.028

6.727 ± 0.055

5.534 ± 0.037

6.554 ± 0.044

9.15 ± 0.055

1.059 ± 0.022

2.643 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski