Batrachochytrium salamandrivorans
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11933 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S8VZV9|A0A1S8VZV9_9FUNG Uncharacterized protein OS=Batrachochytrium salamandrivorans OX=1357716 GN=BSLG_02834 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 5.37 GLDD5 pKa = 5.31 RR6 pKa = 11.84 PDD8 pKa = 4.33 LLDD11 pKa = 3.44 GRR13 pKa = 11.84 TDD15 pKa = 3.0 IGSTEE20 pKa = 4.15 GEE22 pKa = 4.35 DD23 pKa = 4.21 DD24 pKa = 3.67 VSADD28 pKa = 3.87 SEE30 pKa = 4.4 LADD33 pKa = 3.78 TLAQIAAIDD42 pKa = 3.88 EE43 pKa = 4.42 LLAVASSAQSSDD55 pKa = 2.76 LTYY58 pKa = 10.72 PPTEE62 pKa = 4.1 PTSNTITDD70 pKa = 3.86 DD71 pKa = 3.62 PAGISGLEE79 pKa = 3.84 EE80 pKa = 4.81 LIVARR85 pKa = 11.84 NQLVEE90 pKa = 4.17 LADD93 pKa = 4.01 LQRR96 pKa = 11.84 LLLSSAEE103 pKa = 4.25 SVDD106 pKa = 3.73 TSSTAAQHH114 pKa = 5.16 VQSDD118 pKa = 4.33 VAHH121 pKa = 6.79 PSCSIDD127 pKa = 3.07 IGEE130 pKa = 4.76 SSTDD134 pKa = 3.22 NNTDD138 pKa = 3.09 NNADD142 pKa = 3.41 NNTYY146 pKa = 11.06 NNTYY150 pKa = 10.56 NNTYY154 pKa = 10.46 NNTVYY159 pKa = 10.66 PSRR162 pKa = 11.84 PTFAVGGLCCVVFSMSSSQQSFHH185 pKa = 7.17 LLATITSLDD194 pKa = 3.55 QPTNAQRR201 pKa = 11.84 EE202 pKa = 4.17 SDD204 pKa = 4.0 ANLGSDD210 pKa = 4.85 DD211 pKa = 4.08 SDD213 pKa = 3.43 IYY215 pKa = 9.93 ATVMLLTPMTLEE227 pKa = 4.12 TRR229 pKa = 11.84 PCLDD233 pKa = 2.84 IATCSRR239 pKa = 11.84 EE240 pKa = 4.2 CGRR243 pKa = 11.84 SS244 pKa = 3.16
Molecular weight: 25.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.465
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.05
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.808
Patrickios 0.807
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A1S8VUI4|A0A1S8VUI4_9FUNG Uncharacterized protein OS=Batrachochytrium salamandrivorans OX=1357716 GN=BSLG_04310 PE=4 SV=1
RR1 pKa = 7.9 LQIPRR6 pKa = 11.84 MLHH9 pKa = 5.53 QRR11 pKa = 11.84 LHH13 pKa = 5.79 DD14 pKa = 3.32 QYY16 pKa = 10.81 MLHH19 pKa = 6.42 QRR21 pKa = 11.84 LQIPRR26 pKa = 11.84 MLNQHH31 pKa = 6.62 MISQRR36 pKa = 11.84 MLNQHH41 pKa = 6.9 MISQHH46 pKa = 5.49 MLNQHH51 pKa = 6.63 MISQRR56 pKa = 11.84 MVNQRR61 pKa = 11.84 MVNQRR66 pKa = 11.84 MVNQRR71 pKa = 11.84 MVNQRR76 pKa = 11.84 MISQRR81 pKa = 11.84 MISQRR86 pKa = 11.84 MISQRR91 pKa = 11.84 MISQRR96 pKa = 11.84 MINQRR101 pKa = 11.84 MVNQRR106 pKa = 11.84 MVNQRR111 pKa = 11.84 MINQRR116 pKa = 11.84 MVNQRR121 pKa = 11.84 MISQRR126 pKa = 11.84 LQIPRR131 pKa = 11.84 MLHH134 pKa = 5.01 QRR136 pKa = 11.84 LQIPRR141 pKa = 11.84 MLHH144 pKa = 5.01 QRR146 pKa = 11.84 LQIPRR151 pKa = 11.84 MLHH154 pKa = 3.95 QQ155 pKa = 4.25
Molecular weight: 19.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.498
IPC2_protein 11.228
IPC_protein 12.72
Toseland 12.881
ProMoST 13.393
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.384
Grimsley 12.925
Solomon 13.378
Lehninger 13.29
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.106
IPC_peptide 13.393
IPC2_peptide 12.384
IPC2.peptide.svr19 9.192
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10107
1826
11933
5546497
30
5579
464.8
51.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.577 ± 0.021
1.402 ± 0.008
5.765 ± 0.017
5.261 ± 0.02
3.53 ± 0.017
5.665 ± 0.021
2.839 ± 0.013
5.739 ± 0.018
4.96 ± 0.022
9.43 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.008
4.032 ± 0.011
5.39 ± 0.022
4.373 ± 0.022
5.217 ± 0.016
10.107 ± 0.031
6.541 ± 0.018
6.06 ± 0.017
0.994 ± 0.007
2.665 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here