Gordonia phage Kita
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142KB66|A0A142KB66_9CAUD Uncharacterized protein OS=Gordonia phage Kita OX=1821556 GN=36 PE=4 SV=1
MM1 pKa = 7.53 LLDD4 pKa = 3.47 ILAIIVCLVAACWLIATAPDD24 pKa = 4.28 LYY26 pKa = 11.37
Molecular weight: 2.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.218
IPC2_protein 3.757
IPC_protein 3.172
Toseland 3.147
ProMoST 3.63
Dawson 3.325
Bjellqvist 3.567
Wikipedia 3.516
Rodwell 3.16
Grimsley 3.134
Solomon 2.969
Lehninger 2.918
Nozaki 3.63
DTASelect 3.567
Thurlkill 3.363
EMBOSS 3.49
Sillero 3.389
Patrickios 0.006
IPC_peptide 2.956
IPC2_peptide 3.274
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A142KB40|A0A142KB40_9CAUD Uncharacterized protein OS=Gordonia phage Kita OX=1821556 GN=10 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 APLTRR7 pKa = 11.84 SRR9 pKa = 11.84 LSMADD14 pKa = 3.17 LLARR18 pKa = 11.84 FFTQPFTVRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 8.31 TGDD32 pKa = 3.38 GAVGPIYY39 pKa = 10.43 ADD41 pKa = 3.25 PVTLQGRR48 pKa = 11.84 VNATNRR54 pKa = 11.84 LIIDD58 pKa = 3.51 DD59 pKa = 4.68 RR60 pKa = 11.84 GNQVLSAAKK69 pKa = 9.23 ISMSITEE76 pKa = 4.27 HH77 pKa = 7.32 DD78 pKa = 4.29 IPTGSQVRR86 pKa = 11.84 VGDD89 pKa = 4.15 GPWRR93 pKa = 11.84 TVIATSRR100 pKa = 11.84 HH101 pKa = 5.06 RR102 pKa = 11.84 GGFRR106 pKa = 11.84 KK107 pKa = 10.33 SPDD110 pKa = 3.47 YY111 pKa = 11.31 YY112 pKa = 11.38 SIDD115 pKa = 3.74 LNN117 pKa = 3.96
Molecular weight: 12.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.765
IPC_protein 10.906
Toseland 10.877
ProMoST 10.965
Dawson 10.95
Bjellqvist 10.804
Wikipedia 11.286
Rodwell 10.891
Grimsley 11.008
Solomon 11.228
Lehninger 11.155
Nozaki 10.862
DTASelect 10.804
Thurlkill 10.877
EMBOSS 11.316
Sillero 10.906
Patrickios 10.657
IPC_peptide 11.228
IPC2_peptide 9.999
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
16558
26
2145
207.0
22.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.97 ± 0.682
0.755 ± 0.169
7.096 ± 0.397
5.52 ± 0.28
2.579 ± 0.173
8.346 ± 0.371
1.975 ± 0.23
4.499 ± 0.181
3.34 ± 0.215
7.537 ± 0.239
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.102 ± 0.194
3.11 ± 0.201
5.695 ± 0.259
3.744 ± 0.286
7.561 ± 0.432
5.411 ± 0.215
7.416 ± 0.288
7.29 ± 0.236
1.86 ± 0.15
2.192 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here