Pelagibaculum spongiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillales incertae sedis; Pelagibaculum

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1GZJ2|A0A2V1GZJ2_9GAMM DUF3307 domain-containing protein OS=Pelagibaculum spongiae OX=2080658 GN=DC094_03845 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.23KK3 pKa = 10.75GSFLKK8 pKa = 10.48RR9 pKa = 11.84SAVVAFSVLLMAGCGGSGVTDD30 pKa = 3.56TTSGGGTDD38 pKa = 3.62GGTDD42 pKa = 3.5GGTVTPPTTQPFATTCVGTGSSATLGFVNFDD73 pKa = 3.54GASSLEE79 pKa = 4.03LAQKK83 pKa = 10.7GSQSDD88 pKa = 3.64NRR90 pKa = 11.84PQSSTVVFSACSEE103 pKa = 4.39DD104 pKa = 3.58GTPVAGLTVSFSLSTSSGGIAISQASATTDD134 pKa = 3.21ANGQASTTITSGTTATAFILAASVQQSSITEE165 pKa = 3.99TKK167 pKa = 10.33NQVFTIGGSVPDD179 pKa = 4.38ANSFSLSQAATNVDD193 pKa = 3.49GYY195 pKa = 10.96EE196 pKa = 4.02FDD198 pKa = 4.01GATAGINIRR207 pKa = 11.84LADD210 pKa = 3.75HH211 pKa = 6.54VNNPVQDD218 pKa = 3.83GTPVSFITNGGAIQSSCPTALAGGTASCSVTWEE251 pKa = 4.17SQDD254 pKa = 3.61PQPCYY259 pKa = 10.55YY260 pKa = 10.53EE261 pKa = 4.22QVTLSAAQTGLTGIDD276 pKa = 3.05INEE279 pKa = 4.0AVAVCGRR286 pKa = 11.84AAVLAWTEE294 pKa = 4.29GEE296 pKa = 4.27EE297 pKa = 4.92SFNDD301 pKa = 3.46PNQNGVYY308 pKa = 10.21DD309 pKa = 3.9AGEE312 pKa = 4.06TVIGVNNQPGGDD324 pKa = 3.62LAEE327 pKa = 5.4PYY329 pKa = 10.89LDD331 pKa = 3.69VNFNGQYY338 pKa = 10.64DD339 pKa = 3.49IAEE342 pKa = 4.38AFVDD346 pKa = 3.91ANGNSAFDD354 pKa = 3.86PSEE357 pKa = 4.06TYY359 pKa = 10.21TDD361 pKa = 3.66SNNNGVYY368 pKa = 10.48DD369 pKa = 3.83SAEE372 pKa = 4.47PIVDD376 pKa = 3.47RR377 pKa = 11.84DD378 pKa = 3.44GDD380 pKa = 3.94GTRR383 pKa = 11.84TAGDD387 pKa = 3.17AKK389 pKa = 10.32YY390 pKa = 9.07TGLRR394 pKa = 11.84CEE396 pKa = 4.39EE397 pKa = 4.36TNASCTRR404 pKa = 11.84GTLFLYY410 pKa = 9.76DD411 pKa = 3.23QRR413 pKa = 11.84TVVMSGRR420 pKa = 11.84TINARR425 pKa = 11.84ILPASARR432 pKa = 11.84PIIQAAVARR441 pKa = 11.84SSYY444 pKa = 10.54AQTIAILDD452 pKa = 3.88KK453 pKa = 10.89LVAEE457 pKa = 4.65ATPYY461 pKa = 10.35TYY463 pKa = 11.15GDD465 pKa = 3.99EE466 pKa = 4.26LTLLVQDD473 pKa = 4.14QNGNPLAAGTTIAIAAEE490 pKa = 4.3DD491 pKa = 3.68VDD493 pKa = 5.24MIPAAPSATLGQTATLEE510 pKa = 4.34NGQSWLNFTLVQPDD524 pKa = 4.15GGSGTPPEE532 pKa = 4.69EE533 pKa = 4.06GTLTVTITVSRR544 pKa = 11.84ADD546 pKa = 3.62SGVSTLAITYY556 pKa = 9.34VVEE559 pKa = 4.36DD560 pKa = 3.58

Molecular weight: 57.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1GS63|A0A2V1GS63_9GAMM Endonuclease OS=Pelagibaculum spongiae OX=2080658 GN=DC094_17660 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84LVLKK32 pKa = 10.27RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.42GRR39 pKa = 11.84ARR41 pKa = 11.84LAAA44 pKa = 4.44

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4189

0

4189

1371512

26

6845

327.4

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.604 ± 0.043

1.165 ± 0.015

5.605 ± 0.039

5.842 ± 0.043

4.067 ± 0.023

6.694 ± 0.035

2.099 ± 0.018

6.307 ± 0.028

5.387 ± 0.04

10.802 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.023

4.299 ± 0.037

4.141 ± 0.023

5.51 ± 0.057

4.773 ± 0.034

7.074 ± 0.034

4.821 ± 0.028

6.206 ± 0.035

1.34 ± 0.015

2.804 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski