Apis mellifera associated microvirus 11
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8UTE7|A0A3S8UTE7_9VIRU Internal scaffolding protein OS=Apis mellifera associated microvirus 11 OX=2494738 PE=4 SV=1
MM1 pKa = 7.58 AKK3 pKa = 10.22 DD4 pKa = 3.55 LSIPDD9 pKa = 4.48 RR10 pKa = 11.84 IRR12 pKa = 11.84 AHH14 pKa = 7.17 LSTGPYY20 pKa = 9.1 IGRR23 pKa = 11.84 EE24 pKa = 3.95 DD25 pKa = 3.67 WTPALTDD32 pKa = 3.32 EE33 pKa = 5.32 LNRR36 pKa = 11.84 QTAFKK41 pKa = 10.43 SDD43 pKa = 3.57 QEE45 pKa = 4.29 SLTRR49 pKa = 11.84 QEE51 pKa = 5.5 LGDD54 pKa = 3.67 EE55 pKa = 4.3 TDD57 pKa = 3.1 VNRR60 pKa = 11.84 IVQRR64 pKa = 11.84 YY65 pKa = 7.71 GGNVFQADD73 pKa = 3.66 PSRR76 pKa = 11.84 YY77 pKa = 9.8 GFTDD81 pKa = 3.86 DD82 pKa = 4.63 RR83 pKa = 11.84 VDD85 pKa = 3.64 YY86 pKa = 11.09 QKK88 pKa = 11.36 LLEE91 pKa = 4.3 LRR93 pKa = 11.84 EE94 pKa = 4.19 GAAEE98 pKa = 4.05 AFYY101 pKa = 11.1 ALPEE105 pKa = 5.05 DD106 pKa = 3.47 IRR108 pKa = 11.84 ASIGSVEE115 pKa = 4.53 AYY117 pKa = 10.02 LALRR121 pKa = 11.84 MQDD124 pKa = 2.87 VDD126 pKa = 4.06 LDD128 pKa = 4.74 GILGRR133 pKa = 11.84 LEE135 pKa = 4.13 AARR138 pKa = 11.84 KK139 pKa = 9.69 ALDD142 pKa = 3.7 EE143 pKa = 4.48 PLNPPAEE150 pKa = 4.79 PGAGSPPPAAPEE162 pKa = 3.96 AARR165 pKa = 11.84 SAA167 pKa = 3.68
Molecular weight: 18.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.626
IPC2_protein 4.52
IPC_protein 4.444
Toseland 4.279
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.291
Rodwell 4.279
Grimsley 4.177
Solomon 4.406
Lehninger 4.355
Nozaki 4.507
DTASelect 4.698
Thurlkill 4.291
EMBOSS 4.304
Sillero 4.558
Patrickios 4.113
IPC_peptide 4.406
IPC2_peptide 4.546
IPC2.peptide.svr19 4.512
Protein with the highest isoelectric point:
>tr|A0A3Q8U5A0|A0A3Q8U5A0_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 11 OX=2494738 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 PQNTSRR8 pKa = 11.84 SRR10 pKa = 11.84 GANLQRR16 pKa = 11.84 AINEE20 pKa = 3.91 EE21 pKa = 3.92 RR22 pKa = 11.84 EE23 pKa = 3.63 LRR25 pKa = 11.84 ARR27 pKa = 11.84 VGAEE31 pKa = 3.8 EE32 pKa = 4.24 YY33 pKa = 10.97 DD34 pKa = 3.44 RR35 pKa = 11.84 AAAMAEE41 pKa = 3.74 DD42 pKa = 4.68 RR43 pKa = 11.84 NVKK46 pKa = 9.52 QCPCYY51 pKa = 10.78 ACMIRR56 pKa = 11.84 ARR58 pKa = 11.84 FRR60 pKa = 11.84 DD61 pKa = 4.17 TIAAKK66 pKa = 9.86 VWSS69 pKa = 3.93
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 8.931
IPC_protein 9.341
Toseland 9.516
ProMoST 9.443
Dawson 9.823
Bjellqvist 9.736
Wikipedia 10.028
Rodwell 9.867
Grimsley 9.867
Solomon 9.984
Lehninger 9.955
Nozaki 9.882
DTASelect 9.619
Thurlkill 9.692
EMBOSS 9.97
Sillero 9.867
Patrickios 7.614
IPC_peptide 9.97
IPC2_peptide 9.209
IPC2.peptide.svr19 7.67
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1409
69
537
234.8
25.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.13 ± 2.262
0.994 ± 0.473
4.613 ± 1.173
5.678 ± 0.708
3.691 ± 0.651
8.162 ± 0.59
1.419 ± 0.474
4.542 ± 0.568
2.768 ± 0.767
8.091 ± 0.758
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.551
4.4 ± 0.96
6.104 ± 1.013
4.755 ± 0.457
8.517 ± 1.014
5.607 ± 0.357
5.039 ± 0.746
5.465 ± 0.348
1.561 ± 0.463
3.123 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here