Lactobacillus phage JNU_P9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BEZ1|A0A6M3BEZ1_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P9 OX=2686386 PE=4 SV=1
MM1 pKa = 7.1AWVVRR6 pKa = 11.84GEE8 pKa = 3.82EE9 pKa = 3.9AGIIDD14 pKa = 4.47YY15 pKa = 11.27YY16 pKa = 11.07FDD18 pKa = 3.91NLSKK22 pKa = 10.43RR23 pKa = 11.84HH24 pKa = 5.78KK25 pKa = 10.78AVIGDD30 pKa = 3.97FAVTMGDD37 pKa = 3.15PLYY40 pKa = 10.29RR41 pKa = 11.84FKK43 pKa = 11.38SEE45 pKa = 4.69AEE47 pKa = 4.0AEE49 pKa = 4.34TIAMNVYY56 pKa = 10.64YY57 pKa = 9.82DD58 pKa = 4.41DD59 pKa = 4.29EE60 pKa = 6.31DD61 pKa = 3.73MYY63 pKa = 10.93PYY65 pKa = 10.73EE66 pKa = 4.19VTDD69 pKa = 3.89DD70 pKa = 4.03EE71 pKa = 4.76EE72 pKa = 4.35VV73 pKa = 3.0

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BF27|A0A6M3BF27_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P9 OX=2686386 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84GVSFHH7 pKa = 6.47KK8 pKa = 10.44QGNYY12 pKa = 5.14WQMRR16 pKa = 11.84YY17 pKa = 9.61RR18 pKa = 11.84DD19 pKa = 3.5IVEE22 pKa = 4.44HH23 pKa = 6.17YY24 pKa = 10.21KK25 pKa = 10.64SQTEE29 pKa = 4.09AEE31 pKa = 4.4KK32 pKa = 10.63RR33 pKa = 11.84RR34 pKa = 11.84QSLEE38 pKa = 3.78NEE40 pKa = 3.69FGMPEE45 pKa = 3.77RR46 pKa = 11.84YY47 pKa = 10.18GNAKK51 pKa = 9.66KK52 pKa = 10.52DD53 pKa = 3.53YY54 pKa = 10.85SGYY57 pKa = 10.99EE58 pKa = 3.55NDD60 pKa = 3.41NYY62 pKa = 11.17KK63 pKa = 11.03VIGDD67 pKa = 3.97SGKK70 pKa = 9.67ILQNQQVVLALNKK83 pKa = 8.8HH84 pKa = 4.9TNQYY88 pKa = 8.54EE89 pKa = 3.94EE90 pKa = 5.69RR91 pKa = 11.84PIQTIKK97 pKa = 10.44SGKK100 pKa = 9.98ASGFSIRR107 pKa = 11.84KK108 pKa = 9.34AKK110 pKa = 8.69TANLKK115 pKa = 10.52HH116 pKa = 6.26PGVYY120 pKa = 10.01KK121 pKa = 10.7YY122 pKa = 10.65DD123 pKa = 3.62NHH125 pKa = 6.92WIATITIEE133 pKa = 4.54GKK135 pKa = 10.43GYY137 pKa = 10.15HH138 pKa = 6.32LGSFEE143 pKa = 5.64SKK145 pKa = 9.71DD146 pKa = 3.36QGRR149 pKa = 11.84KK150 pKa = 8.77VYY152 pKa = 10.63QKK154 pKa = 11.25ALEE157 pKa = 3.89EE158 pKa = 4.67WITKK162 pKa = 9.53GIKK165 pKa = 9.02PYY167 pKa = 10.64KK168 pKa = 8.19VTPHH172 pKa = 6.43PRR174 pKa = 11.84STNTSGEE181 pKa = 4.09LYY183 pKa = 9.59IQYY186 pKa = 10.43RR187 pKa = 11.84SNRR190 pKa = 11.84KK191 pKa = 7.44PKK193 pKa = 8.2PWVFEE198 pKa = 3.98KK199 pKa = 10.08VTSGKK204 pKa = 10.54RR205 pKa = 11.84ITRR208 pKa = 11.84YY209 pKa = 9.85FSTLAEE215 pKa = 4.82AISFKK220 pKa = 10.68HH221 pKa = 6.36KK222 pKa = 10.87YY223 pKa = 10.16LGGNN227 pKa = 3.41

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12462

42

1308

222.5

24.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.682 ± 0.51

0.562 ± 0.103

6.917 ± 0.383

5.208 ± 0.354

3.266 ± 0.199

6.829 ± 0.494

1.894 ± 0.165

6.139 ± 0.174

7.294 ± 0.402

7.455 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.175

5.288 ± 0.237

3.386 ± 0.218

4.686 ± 0.28

3.82 ± 0.353

7.334 ± 0.288

6.452 ± 0.354

6.371 ± 0.24

1.613 ± 0.166

4.076 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski