Lactobacillus phage JNU_P9
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M3BEZ1|A0A6M3BEZ1_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P9 OX=2686386 PE=4 SV=1
MM1 pKa = 7.1 AWVVRR6 pKa = 11.84 GEE8 pKa = 3.82 EE9 pKa = 3.9 AGIIDD14 pKa = 4.47 YY15 pKa = 11.27 YY16 pKa = 11.07 FDD18 pKa = 3.91 NLSKK22 pKa = 10.43 RR23 pKa = 11.84 HH24 pKa = 5.78 KK25 pKa = 10.78 AVIGDD30 pKa = 3.97 FAVTMGDD37 pKa = 3.15 PLYY40 pKa = 10.29 RR41 pKa = 11.84 FKK43 pKa = 11.38 SEE45 pKa = 4.69 AEE47 pKa = 4.0 AEE49 pKa = 4.34 TIAMNVYY56 pKa = 10.64 YY57 pKa = 9.82 DD58 pKa = 4.41 DD59 pKa = 4.29 EE60 pKa = 6.31 DD61 pKa = 3.73 MYY63 pKa = 10.93 PYY65 pKa = 10.73 EE66 pKa = 4.19 VTDD69 pKa = 3.89 DD70 pKa = 4.03 EE71 pKa = 4.76 EE72 pKa = 4.35 VV73 pKa = 3.0
Molecular weight: 8.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.745
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.795
EMBOSS 3.834
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|A0A6M3BF27|A0A6M3BF27_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P9 OX=2686386 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 GVSFHH7 pKa = 6.47 KK8 pKa = 10.44 QGNYY12 pKa = 5.14 WQMRR16 pKa = 11.84 YY17 pKa = 9.61 RR18 pKa = 11.84 DD19 pKa = 3.5 IVEE22 pKa = 4.44 HH23 pKa = 6.17 YY24 pKa = 10.21 KK25 pKa = 10.64 SQTEE29 pKa = 4.09 AEE31 pKa = 4.4 KK32 pKa = 10.63 RR33 pKa = 11.84 RR34 pKa = 11.84 QSLEE38 pKa = 3.78 NEE40 pKa = 3.69 FGMPEE45 pKa = 3.77 RR46 pKa = 11.84 YY47 pKa = 10.18 GNAKK51 pKa = 9.66 KK52 pKa = 10.52 DD53 pKa = 3.53 YY54 pKa = 10.85 SGYY57 pKa = 10.99 EE58 pKa = 3.55 NDD60 pKa = 3.41 NYY62 pKa = 11.17 KK63 pKa = 11.03 VIGDD67 pKa = 3.97 SGKK70 pKa = 9.67 ILQNQQVVLALNKK83 pKa = 8.8 HH84 pKa = 4.9 TNQYY88 pKa = 8.54 EE89 pKa = 3.94 EE90 pKa = 5.69 RR91 pKa = 11.84 PIQTIKK97 pKa = 10.44 SGKK100 pKa = 9.98 ASGFSIRR107 pKa = 11.84 KK108 pKa = 9.34 AKK110 pKa = 8.69 TANLKK115 pKa = 10.52 HH116 pKa = 6.26 PGVYY120 pKa = 10.01 KK121 pKa = 10.7 YY122 pKa = 10.65 DD123 pKa = 3.62 NHH125 pKa = 6.92 WIATITIEE133 pKa = 4.54 GKK135 pKa = 10.43 GYY137 pKa = 10.15 HH138 pKa = 6.32 LGSFEE143 pKa = 5.64 SKK145 pKa = 9.71 DD146 pKa = 3.36 QGRR149 pKa = 11.84 KK150 pKa = 8.77 VYY152 pKa = 10.63 QKK154 pKa = 11.25 ALEE157 pKa = 3.89 EE158 pKa = 4.67 WITKK162 pKa = 9.53 GIKK165 pKa = 9.02 PYY167 pKa = 10.64 KK168 pKa = 8.19 VTPHH172 pKa = 6.43 PRR174 pKa = 11.84 STNTSGEE181 pKa = 4.09 LYY183 pKa = 9.59 IQYY186 pKa = 10.43 RR187 pKa = 11.84 SNRR190 pKa = 11.84 KK191 pKa = 7.44 PKK193 pKa = 8.2 PWVFEE198 pKa = 3.98 KK199 pKa = 10.08 VTSGKK204 pKa = 10.54 RR205 pKa = 11.84 ITRR208 pKa = 11.84 YY209 pKa = 9.85 FSTLAEE215 pKa = 4.82 AISFKK220 pKa = 10.68 HH221 pKa = 6.36 KK222 pKa = 10.87 YY223 pKa = 10.16 LGGNN227 pKa = 3.41
Molecular weight: 26.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.123
IPC2_protein 9.414
IPC_protein 9.341
Toseland 9.94
ProMoST 9.648
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.35
Rodwell 10.613
Grimsley 10.248
Solomon 10.189
Lehninger 10.145
Nozaki 9.911
DTASelect 9.838
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.072
Patrickios 9.897
IPC_peptide 10.189
IPC2_peptide 8.331
IPC2.peptide.svr19 8.416
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
12462
42
1308
222.5
24.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.682 ± 0.51
0.562 ± 0.103
6.917 ± 0.383
5.208 ± 0.354
3.266 ± 0.199
6.829 ± 0.494
1.894 ± 0.165
6.139 ± 0.174
7.294 ± 0.402
7.455 ± 0.272
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.728 ± 0.175
5.288 ± 0.237
3.386 ± 0.218
4.686 ± 0.28
3.82 ± 0.353
7.334 ± 0.288
6.452 ± 0.354
6.371 ± 0.24
1.613 ± 0.166
4.076 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here