Arthrobacter sp. NEB 688

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M8H6I3|A0A6M8H6I3_9MICC ABC transporter ATP-binding protein OS=Arthrobacter sp. NEB 688 OX=904039 GN=HL663_02540 PE=4 SV=1
MM1 pKa = 7.9SDD3 pKa = 3.44LEE5 pKa = 4.37YY6 pKa = 10.11PAGLRR11 pKa = 11.84YY12 pKa = 7.7TAEE15 pKa = 4.27HH16 pKa = 5.42EE17 pKa = 4.53WVRR20 pKa = 11.84EE21 pKa = 3.71EE22 pKa = 4.23AGVVRR27 pKa = 11.84IGITAFAQEE36 pKa = 4.0ALGDD40 pKa = 3.84VVYY43 pKa = 11.21VSLPAVGTTVTAGDD57 pKa = 3.41TCGEE61 pKa = 4.21VEE63 pKa = 4.37STKK66 pKa = 10.75SVSDD70 pKa = 3.79LYY72 pKa = 11.45APVAGEE78 pKa = 3.9VTAVNDD84 pKa = 4.22ALDD87 pKa = 3.97ATPEE91 pKa = 4.17LVNSDD96 pKa = 4.01PYY98 pKa = 11.46GEE100 pKa = 3.73GWMYY104 pKa = 10.45EE105 pKa = 4.25LRR107 pKa = 11.84PADD110 pKa = 4.09DD111 pKa = 3.95AAVAALLDD119 pKa = 3.5VDD121 pKa = 4.89AYY123 pKa = 10.62RR124 pKa = 11.84AQLSS128 pKa = 3.56

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M8GKW3|A0A6M8GKW3_9MICC Mycothiol system anti-sigma-R factor OS=Arthrobacter sp. NEB 688 OX=904039 GN=HL663_08965 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3724

0

3724

1223339

31

2118

328.5

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.77 ± 0.053

0.654 ± 0.01

6.339 ± 0.032

5.539 ± 0.04

2.524 ± 0.028

9.588 ± 0.035

2.203 ± 0.023

2.596 ± 0.03

1.623 ± 0.032

10.418 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.712 ± 0.018

1.436 ± 0.024

5.879 ± 0.033

2.45 ± 0.02

8.185 ± 0.049

5.12 ± 0.031

6.303 ± 0.038

10.359 ± 0.045

1.574 ± 0.016

1.728 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski