Mycobacterium phage Pipefish

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pipefishvirus; Mycobacterium virus Pipefish

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19YQ8|Q19YQ8_9CAUD Uncharacterized protein OS=Mycobacterium phage Pipefish OX=373413 GN=97 PE=4 SV=1
MM1 pKa = 7.18AQDD4 pKa = 3.55VNLGAVFDD12 pKa = 4.7DD13 pKa = 4.29ALTAEE18 pKa = 4.43EE19 pKa = 4.23WWDD22 pKa = 3.53EE23 pKa = 4.2FNANACCPDD32 pKa = 3.74SVTAAARR39 pKa = 11.84LCGCGGSGRR48 pKa = 11.84IPTGISRR55 pKa = 11.84LLL57 pKa = 3.6

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19YX7|Q19YX7_9CAUD Tape measure protein OS=Mycobacterium phage Pipefish OX=373413 GN=28 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 4.53RR3 pKa = 11.84WKK5 pKa = 10.51PIDD8 pKa = 3.42GWPGYY13 pKa = 9.92EE14 pKa = 4.43ISDD17 pKa = 3.78HH18 pKa = 6.32GNVRR22 pKa = 11.84SIDD25 pKa = 3.6RR26 pKa = 11.84EE27 pKa = 4.05VSNGRR32 pKa = 11.84GTHH35 pKa = 4.96ILKK38 pKa = 10.74GKK40 pKa = 9.91LLVPFVTKK48 pKa = 10.49KK49 pKa = 10.34GYY51 pKa = 10.35HH52 pKa = 4.97SVGLRR57 pKa = 11.84RR58 pKa = 11.84DD59 pKa = 3.31GKK61 pKa = 7.25YY62 pKa = 9.24TRR64 pKa = 11.84FLVHH68 pKa = 6.72RR69 pKa = 11.84LVYY72 pKa = 10.0SHH74 pKa = 7.31FVGDD78 pKa = 3.86CTGLQVRR85 pKa = 11.84HH86 pKa = 5.75WPDD89 pKa = 3.29RR90 pKa = 11.84DD91 pKa = 3.6PSHH94 pKa = 6.31NTPDD98 pKa = 3.54NLTVGTNSDD107 pKa = 3.44NQRR110 pKa = 11.84DD111 pKa = 4.17SVAHH115 pKa = 5.08GTHH118 pKa = 6.29RR119 pKa = 11.84SARR122 pKa = 11.84KK123 pKa = 6.78TEE125 pKa = 4.48CIRR128 pKa = 11.84GHH130 pKa = 6.96AFTPANTLHH139 pKa = 6.24YY140 pKa = 9.74VGKK143 pKa = 10.13SGPARR148 pKa = 11.84SCRR151 pKa = 11.84RR152 pKa = 11.84CHH154 pKa = 7.49ADD156 pKa = 3.23RR157 pKa = 11.84QKK159 pKa = 11.44ARR161 pKa = 11.84RR162 pKa = 11.84HH163 pKa = 4.7RR164 pKa = 3.79

Molecular weight: 18.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

21919

34

1910

214.9

23.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.346 ± 0.3

1.136 ± 0.131

6.866 ± 0.232

5.922 ± 0.282

2.655 ± 0.111

9.435 ± 0.636

1.802 ± 0.16

4.786 ± 0.186

3.216 ± 0.154

7.797 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.126

2.929 ± 0.134

6.072 ± 0.228

3.038 ± 0.146

6.839 ± 0.444

5.525 ± 0.186

6.547 ± 0.197

7.833 ± 0.247

1.934 ± 0.142

2.185 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski