Murine hepatitis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Murine coronavirus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77NL4|Q77NL4_9BETC Non-structural protein 2a OS=Murine hepatitis virus OX=11138 GN=ORF2A PE=3 SV=1
MM1 pKa = 7.46FNLFLTDD8 pKa = 3.48TVWYY12 pKa = 8.66VGQIIFIFAVCLMVTIIVVAFLASIKK38 pKa = 10.74LCIQLCGLCNTLVLSPSIYY57 pKa = 10.4LYY59 pKa = 10.85DD60 pKa = 4.56RR61 pKa = 11.84SKK63 pKa = 11.14QLYY66 pKa = 8.86KK67 pKa = 10.82YY68 pKa = 9.71YY69 pKa = 10.26NEE71 pKa = 3.95EE72 pKa = 3.47MRR74 pKa = 11.84LPLLEE79 pKa = 4.79VDD81 pKa = 5.76DD82 pKa = 4.29II83 pKa = 4.66

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9J3E3|Q9J3E3_9BETC Membrane protein OS=Murine hepatitis virus OX=11138 GN=ORF6 PE=3 SV=1
MM1 pKa = 7.35SFVPGQEE8 pKa = 3.97NAGGRR13 pKa = 11.84SSSVNRR19 pKa = 11.84AGNGILKK26 pKa = 8.59KK27 pKa = 7.37TTWADD32 pKa = 3.24QTEE35 pKa = 4.28RR36 pKa = 11.84GPNNQNRR43 pKa = 11.84GRR45 pKa = 11.84RR46 pKa = 11.84NQPKK50 pKa = 8.18QTATTQPNSGSVVPHH65 pKa = 5.66YY66 pKa = 11.05SWFSGITQFQKK77 pKa = 10.82GKK79 pKa = 9.62EE80 pKa = 3.93FQFAEE85 pKa = 4.59GQGVPIANGIPASEE99 pKa = 4.21QKK101 pKa = 10.87GYY103 pKa = 9.69WYY105 pKa = 10.34RR106 pKa = 11.84HH107 pKa = 4.36NRR109 pKa = 11.84RR110 pKa = 11.84SFKK113 pKa = 10.75TPDD116 pKa = 3.34GQQKK120 pKa = 9.76QLLPRR125 pKa = 11.84WYY127 pKa = 9.79FYY129 pKa = 11.56YY130 pKa = 10.78LGTGPHH136 pKa = 6.72AGASYY141 pKa = 10.53GDD143 pKa = 4.18SIEE146 pKa = 4.14GVFWVANSQADD157 pKa = 3.73TNTRR161 pKa = 11.84SDD163 pKa = 3.07IVEE166 pKa = 4.41RR167 pKa = 11.84DD168 pKa = 3.66PSSHH172 pKa = 5.75EE173 pKa = 4.53AIPTRR178 pKa = 11.84FAPGTVLPQGFYY190 pKa = 10.92VEE192 pKa = 4.42GSGRR196 pKa = 11.84SAPASRR202 pKa = 11.84SGSRR206 pKa = 11.84SQSRR210 pKa = 11.84GPNNRR215 pKa = 11.84ARR217 pKa = 11.84SSSNQRR223 pKa = 11.84QPASTVKK230 pKa = 10.23PDD232 pKa = 3.37MAEE235 pKa = 4.03EE236 pKa = 3.7IAALVLAKK244 pKa = 10.33LGKK247 pKa = 10.0DD248 pKa = 2.88AGQPKK253 pKa = 9.61QVTKK257 pKa = 10.71QSAKK261 pKa = 8.91EE262 pKa = 3.89VRR264 pKa = 11.84QKK266 pKa = 10.73ILNKK270 pKa = 9.17PRR272 pKa = 11.84QKK274 pKa = 9.65RR275 pKa = 11.84TPNKK279 pKa = 8.97QCPVQQCFGKK289 pKa = 10.52RR290 pKa = 11.84GPNQNFGGSEE300 pKa = 3.94MLKK303 pKa = 10.6LGTSDD308 pKa = 3.38PQFPILAEE316 pKa = 3.97LAPTVGAFFFGSKK329 pKa = 10.69LEE331 pKa = 4.03LVKK334 pKa = 10.9KK335 pKa = 10.59NSGGADD341 pKa = 3.41EE342 pKa = 4.48PTKK345 pKa = 10.65DD346 pKa = 3.71VYY348 pKa = 10.84EE349 pKa = 4.14LQYY352 pKa = 11.13SGAVRR357 pKa = 11.84FDD359 pKa = 3.21STLPGFEE366 pKa = 4.83TIMKK370 pKa = 9.51VLNEE374 pKa = 4.26NLNAYY379 pKa = 9.5QKK381 pKa = 11.1DD382 pKa = 3.25GGADD386 pKa = 3.39VVSPKK391 pKa = 9.52PQRR394 pKa = 11.84KK395 pKa = 8.65GRR397 pKa = 11.84RR398 pKa = 11.84QALEE402 pKa = 4.0KK403 pKa = 10.3KK404 pKa = 10.6DD405 pKa = 3.86EE406 pKa = 4.05VDD408 pKa = 3.31NVSVAKK414 pKa = 10.14PKK416 pKa = 10.81SSVQRR421 pKa = 11.84NVSRR425 pKa = 11.84EE426 pKa = 4.02LTPEE430 pKa = 4.02DD431 pKa = 4.12RR432 pKa = 11.84SLLAQILDD440 pKa = 4.15DD441 pKa = 4.33GVVPDD446 pKa = 4.88GLEE449 pKa = 4.2DD450 pKa = 4.13DD451 pKa = 4.85SNVV454 pKa = 3.06

Molecular weight: 49.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

9787

19

4467

978.7

109.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.805 ± 0.355

3.75 ± 0.329

5.589 ± 0.28

4.23 ± 0.186

5.344 ± 0.142

6.151 ± 0.293

1.778 ± 0.187

4.455 ± 0.493

5.712 ± 0.361

9.094 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.233

5.099 ± 0.611

3.832 ± 0.323

3.505 ± 0.374

3.852 ± 0.402

7.101 ± 0.266

5.783 ± 0.267

9.666 ± 0.93

1.318 ± 0.117

4.782 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski