Mycobacterium celatum
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4310 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X1RW58|A0A1X1RW58_MYCCE Uncharacterized protein OS=Mycobacterium celatum OX=28045 GN=AWB95_00020 PE=4 SV=1
MM1 pKa = 7.69 TDD3 pKa = 3.3 YY4 pKa = 11.52 KK5 pKa = 11.3 LFMCVQCGFEE15 pKa = 3.97 YY16 pKa = 10.91 DD17 pKa = 3.75 EE18 pKa = 4.64 AKK20 pKa = 10.38 GWPEE24 pKa = 4.27 DD25 pKa = 4.17 GIAPGTRR32 pKa = 11.84 WDD34 pKa = 5.68 DD35 pKa = 4.13 IPDD38 pKa = 3.72 DD39 pKa = 4.45 WSCPDD44 pKa = 3.66 CGAAKK49 pKa = 10.31 SDD51 pKa = 3.66 FEE53 pKa = 4.32 MVEE56 pKa = 4.02 VARR59 pKa = 11.84 AA60 pKa = 3.1
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A1X1RXF1|A0A1X1RXF1_MYCCE Uncharacterized protein OS=Mycobacterium celatum OX=28045 GN=AWB95_00570 PE=4 SV=1
MM1 pKa = 5.98 TTKK4 pKa = 10.19 PRR6 pKa = 11.84 RR7 pKa = 11.84 SVNLRR12 pKa = 11.84 ILPSSMSVLAICAGLSSIRR31 pKa = 11.84 FALEE35 pKa = 4.07 AQPHH39 pKa = 5.84 AAMALIAAAAILDD52 pKa = 4.04 GLDD55 pKa = 3.18 GRR57 pKa = 11.84 VARR60 pKa = 11.84 ILDD63 pKa = 3.53 AEE65 pKa = 4.23 SRR67 pKa = 11.84 MGEE70 pKa = 4.31 EE71 pKa = 4.16 IDD73 pKa = 4.3 SLADD77 pKa = 3.31 AVNFGVAPAVVVYY90 pKa = 9.84 VSLLASLPAGWVAVLLYY107 pKa = 10.19 AVCIVLRR114 pKa = 11.84 LARR117 pKa = 11.84 FNALLDD123 pKa = 4.31 DD124 pKa = 4.37 ATLPAYY130 pKa = 9.29 TRR132 pKa = 11.84 EE133 pKa = 4.3 FFVGMPAPAGAIAVLGPLAAKK154 pKa = 10.05 LQFGVGWWSSPWFVCAWVVVCSMLVVSRR182 pKa = 11.84 IPMKK186 pKa = 10.54 KK187 pKa = 7.52 MHH189 pKa = 6.54 AVSVPPNFAALLLAVLAIVAAAAFLFPYY217 pKa = 9.23 ILVMVAIAAYY227 pKa = 9.43 LCHH230 pKa = 6.95 IPFSVRR236 pKa = 11.84 SHH238 pKa = 6.07 RR239 pKa = 11.84 WLAEE243 pKa = 3.75 HH244 pKa = 6.94 PEE246 pKa = 4.13 VWGEE250 pKa = 3.86 RR251 pKa = 11.84 PKK253 pKa = 10.76 EE254 pKa = 3.68 RR255 pKa = 11.84 RR256 pKa = 11.84 AVRR259 pKa = 11.84 RR260 pKa = 11.84 AIRR263 pKa = 11.84 RR264 pKa = 11.84 AQPNRR269 pKa = 11.84 RR270 pKa = 11.84 SMARR274 pKa = 11.84 LGLRR278 pKa = 11.84 KK279 pKa = 9.67 PGGG282 pKa = 3.38
Molecular weight: 30.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.531
IPC_protein 10.438
Toseland 10.452
ProMoST 10.233
Dawson 10.599
Bjellqvist 10.365
Wikipedia 10.833
Rodwell 10.687
Grimsley 10.657
Solomon 10.701
Lehninger 10.672
Nozaki 10.496
DTASelect 10.335
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.365
IPC_peptide 10.701
IPC2_peptide 9.648
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4310
0
4310
1386883
37
4338
321.8
34.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.133 ± 0.053
0.838 ± 0.01
6.265 ± 0.034
5.251 ± 0.028
3.034 ± 0.02
8.697 ± 0.035
2.26 ± 0.017
4.2 ± 0.024
2.27 ± 0.024
9.879 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.044 ± 0.016
2.197 ± 0.02
5.928 ± 0.037
3.067 ± 0.021
7.444 ± 0.035
5.267 ± 0.024
5.804 ± 0.028
8.679 ± 0.034
1.534 ± 0.015
2.21 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here