Chickpea chlorotic stunt virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q17S72|Q17S72_9LUTE p0 protein OS=Chickpea chlorotic stunt virus OX=328430 GN=p0 PE=4 SV=1
MM1 pKa = 7.29NVLINKK7 pKa = 6.81FTSNFEE13 pKa = 4.54INFSPSLSLATRR25 pKa = 11.84RR26 pKa = 11.84SLICLFLANSSEE38 pKa = 4.32FIKK41 pKa = 10.61ISNEE45 pKa = 3.39RR46 pKa = 11.84DD47 pKa = 3.39GTDD50 pKa = 2.73KK51 pKa = 10.4STCYY55 pKa = 10.13RR56 pKa = 11.84SLLLLLPFLFGSFEE70 pKa = 4.07FFGDD74 pKa = 3.54QLVVPWNGGNNRR86 pKa = 11.84HH87 pKa = 6.39HH88 pKa = 7.13GLFQRR93 pKa = 11.84LLTNTRR99 pKa = 11.84FCTLRR104 pKa = 11.84LQPGRR109 pKa = 11.84EE110 pKa = 3.74ARR112 pKa = 11.84YY113 pKa = 9.77NPSIQITPTRR123 pKa = 11.84VEE125 pKa = 4.35LYY127 pKa = 10.21RR128 pKa = 11.84YY129 pKa = 8.4IQSPLVEE136 pKa = 4.51GLSGHH141 pKa = 6.32QDD143 pKa = 3.49LLSLGLHH150 pKa = 6.61HH151 pKa = 6.43FTKK154 pKa = 10.87LVGAYY159 pKa = 9.19VRR161 pKa = 11.84QLEE164 pKa = 4.34RR165 pKa = 11.84NNFCLRR171 pKa = 11.84SEE173 pKa = 4.7IILGDD178 pKa = 3.95SVDD181 pKa = 4.61LDD183 pKa = 3.94VSHH186 pKa = 7.36LGILLMDD193 pKa = 4.84GSDD196 pKa = 4.14HH197 pKa = 7.02NNTHH201 pKa = 6.47HH202 pKa = 7.17AYY204 pKa = 10.19SGSRR208 pKa = 11.84VINCLHH214 pKa = 6.5RR215 pKa = 11.84CYY217 pKa = 10.72GQALTSDD224 pKa = 2.86IWEE227 pKa = 4.39LFGVDD232 pKa = 4.03YY233 pKa = 10.37CPCLQVPSFPLEE245 pKa = 3.82AEE247 pKa = 3.89IPEE250 pKa = 4.94DD251 pKa = 3.53SFQFDD256 pKa = 3.72EE257 pKa = 5.04DD258 pKa = 4.42SEE260 pKa = 5.18GEE262 pKa = 3.92DD263 pKa = 5.63DD264 pKa = 4.05EE265 pKa = 6.16GFWEE269 pKa = 4.36LL270 pKa = 4.69

Molecular weight: 30.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q17S69|Q17S69_9LUTE Readthrough protein OS=Chickpea chlorotic stunt virus OX=328430 GN=p5 PE=3 SV=1
MM1 pKa = 6.53NTVVVRR7 pKa = 11.84NNGRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84TVQRR21 pKa = 11.84ARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84NPVVVVEE32 pKa = 5.36APRR35 pKa = 11.84QPQRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84NRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84ASGRR53 pKa = 11.84STAGRR58 pKa = 11.84RR59 pKa = 11.84GSSEE63 pKa = 3.82TFVFSKK69 pKa = 11.13DD70 pKa = 3.3NLAGSSSGSITFGPSLSDD88 pKa = 3.49CPAFSSGILRR98 pKa = 11.84AYY100 pKa = 10.24HH101 pKa = 6.47EE102 pKa = 4.67YY103 pKa = 9.99KK104 pKa = 10.23ISMVKK109 pKa = 10.66LEE111 pKa = 5.26FISEE115 pKa = 4.18AASTSSGSIAYY126 pKa = 9.43EE127 pKa = 3.86LDD129 pKa = 3.43PHH131 pKa = 6.83CKK133 pKa = 8.5STSLGSYY140 pKa = 9.12INKK143 pKa = 9.91FGITSNGQRR152 pKa = 11.84TFAARR157 pKa = 11.84LINGIEE163 pKa = 3.93WHH165 pKa = 6.9SSDD168 pKa = 3.83EE169 pKa = 4.08DD170 pKa = 3.46QFRR173 pKa = 11.84ILYY176 pKa = 9.29KK177 pKa = 10.97GNGGSAIAGSFRR189 pKa = 11.84ITIKK193 pKa = 10.84CQTQNPKK200 pKa = 10.57

Molecular weight: 22.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3109

193

1093

518.2

58.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.143 ± 0.448

1.866 ± 0.249

4.439 ± 0.418

6.819 ± 0.515

4.085 ± 0.414

7.044 ± 0.393

1.994 ± 0.286

4.728 ± 0.251

6.143 ± 0.848

8.684 ± 1.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.263

4.76 ± 0.377

5.05 ± 0.457

3.763 ± 0.206

6.304 ± 1.119

9.682 ± 0.71

5.436 ± 0.462

5.886 ± 0.449

1.994 ± 0.254

3.249 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski