Acinetobacter phage YMC11/11/R3177
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D4DBL3|A0A0D4DBL3_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC11/11/R3177 OX=1628721 GN=ABA3177_00530 PE=4 SV=1
MM1 pKa = 7.66 INNNYY6 pKa = 8.27 VPQWHH11 pKa = 5.9 EE12 pKa = 4.04 TPFQHH17 pKa = 6.94 IKK19 pKa = 8.45 YY20 pKa = 8.13 TLVRR24 pKa = 11.84 NQDD27 pKa = 3.26 QLDD30 pKa = 3.49 ILFDD34 pKa = 3.98 SVKK37 pKa = 10.6 APYY40 pKa = 10.19 EE41 pKa = 3.91 FLEE44 pKa = 4.85 GGADD48 pKa = 3.37 ARR50 pKa = 11.84 VNFQDD55 pKa = 4.03 GYY57 pKa = 11.48 AVVQIGDD64 pKa = 3.81 CSNWDD69 pKa = 4.3 LIQVHH74 pKa = 6.34 GLLLHH79 pKa = 6.08 EE80 pKa = 5.42 AVHH83 pKa = 6.15 IWQEE87 pKa = 3.84 VALVMGEE94 pKa = 4.21 EE95 pKa = 4.12 NPSIEE100 pKa = 4.05 FEE102 pKa = 4.33 AYY104 pKa = 10.11 SIQAIAQDD112 pKa = 3.9 LFEE115 pKa = 4.67 MFEE118 pKa = 4.23 ASTTRR123 pKa = 3.26
Molecular weight: 14.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.013
IPC2_protein 4.317
IPC_protein 4.228
Toseland 4.062
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.062
Grimsley 3.986
Solomon 4.177
Lehninger 4.126
Nozaki 4.304
DTASelect 4.444
Thurlkill 4.088
EMBOSS 4.088
Sillero 4.342
Patrickios 1.964
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.225
Protein with the highest isoelectric point:
>tr|A0A0D4DBJ3|A0A0D4DBJ3_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC11/11/R3177 OX=1628721 GN=ABA3177_00330 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 YY3 pKa = 9.52 AVRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.59 QDD10 pKa = 2.92 ISVSTTPLEE19 pKa = 4.04 VVIPLEE25 pKa = 4.17 QPVKK29 pKa = 10.13 IYY31 pKa = 10.01 SAKK34 pKa = 9.98 EE35 pKa = 3.5 LAAMPLSVMNAAIEE49 pKa = 4.11 AQEE52 pKa = 4.02 RR53 pKa = 11.84 FYY55 pKa = 11.0 QLEE58 pKa = 4.44 EE59 pKa = 4.04 LTHH62 pKa = 6.12 MGGQAIAVRR71 pKa = 11.84 RR72 pKa = 11.84 LMEE75 pKa = 5.45 DD76 pKa = 3.03 GHH78 pKa = 8.51 KK79 pKa = 10.47 LIQVKK84 pKa = 9.57 EE85 pKa = 4.19 KK86 pKa = 10.82 SRR88 pKa = 11.84 IRR90 pKa = 11.84 YY91 pKa = 8.65 KK92 pKa = 10.57 INNEE96 pKa = 4.49 FIPPRR101 pKa = 11.84 IIRR104 pKa = 11.84 QLEE107 pKa = 3.67 MRR109 pKa = 11.84 GLVKK113 pKa = 10.76 LGRR116 pKa = 11.84 GKK118 pKa = 10.62
Molecular weight: 13.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.174
IPC2_protein 9.414
IPC_protein 9.589
Toseland 10.262
ProMoST 9.94
Dawson 10.409
Bjellqvist 10.043
Wikipedia 10.57
Rodwell 10.818
Grimsley 10.467
Solomon 10.452
Lehninger 10.423
Nozaki 10.218
DTASelect 10.058
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.321
Patrickios 10.54
IPC_peptide 10.452
IPC2_peptide 8.551
IPC2.peptide.svr19 8.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
14276
37
1436
178.4
20.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.441 ± 0.512
1.03 ± 0.131
5.905 ± 0.167
7.404 ± 0.338
3.762 ± 0.19
6.542 ± 0.29
1.898 ± 0.189
5.933 ± 0.211
7.754 ± 0.417
8.413 ± 0.209
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.466 ± 0.16
5.043 ± 0.237
3.502 ± 0.278
4.651 ± 0.309
4.609 ± 0.177
5.954 ± 0.256
5.366 ± 0.325
6.367 ± 0.267
1.254 ± 0.126
3.706 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here