Dickeya phage BF25/12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Stompvirus; Dickeya virus BF25-12

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219MH13|A0A219MH13_9CAUD Putative DNA exonuclease OS=Dickeya phage BF25/12 OX=1698708 GN=BF2512_30 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.23ITIAEE7 pKa = 4.14PVLINPLEE15 pKa = 4.12QFKK18 pKa = 9.01PTVATNTRR26 pKa = 11.84KK27 pKa = 9.93LLAEE31 pKa = 4.15IFLARR36 pKa = 11.84DD37 pKa = 3.52TTTVEE42 pKa = 3.87AQPLDD47 pKa = 3.8KK48 pKa = 10.9EE49 pKa = 4.56VLKK52 pKa = 10.78EE53 pKa = 3.95MKK55 pKa = 10.06QLVYY59 pKa = 9.99TGCDD63 pKa = 2.75NKK65 pKa = 10.2MVSQYY70 pKa = 10.87SEE72 pKa = 4.29HH73 pKa = 7.28PSPEE77 pKa = 3.52AHH79 pKa = 6.68MLRR82 pKa = 11.84AVGVAASVADD92 pKa = 3.95FEE94 pKa = 5.08EE95 pKa = 4.25NRR97 pKa = 11.84EE98 pKa = 3.47GSYY101 pKa = 9.95RR102 pKa = 11.84VEE104 pKa = 5.77FAFQWLRR111 pKa = 11.84GYY113 pKa = 10.35LFKK116 pKa = 11.43NNLPMPTEE124 pKa = 4.66FPANTLGALSYY135 pKa = 9.75PARR138 pKa = 11.84TCCCMDD144 pKa = 5.04DD145 pKa = 4.15YY146 pKa = 11.03LSSMRR151 pKa = 11.84VIPSKK156 pKa = 10.78LLYY159 pKa = 9.45HH160 pKa = 6.87LKK162 pKa = 10.33EE163 pKa = 4.08AVPPVLLAMYY173 pKa = 8.66EE174 pKa = 4.15PRR176 pKa = 11.84TQSYY180 pKa = 9.04YY181 pKa = 11.33GKK183 pKa = 10.26DD184 pKa = 2.98LVEE187 pKa = 4.63RR188 pKa = 11.84QTEE191 pKa = 4.13VNNIGGYY198 pKa = 9.79CGVVYY203 pKa = 10.53TEE205 pKa = 6.08DD206 pKa = 3.21GTAWFAGFLNADD218 pKa = 3.94DD219 pKa = 3.87VLKK222 pKa = 11.09LRR224 pKa = 11.84FRR226 pKa = 11.84RR227 pKa = 11.84MRR229 pKa = 11.84LGAAIKK235 pKa = 10.33EE236 pKa = 4.17LTGDD240 pKa = 3.72DD241 pKa = 3.53NLARR245 pKa = 11.84KK246 pKa = 8.86AADD249 pKa = 3.09IRR251 pKa = 11.84DD252 pKa = 3.63LSSYY256 pKa = 11.48DD257 pKa = 4.4LVLHH261 pKa = 6.5PNDD264 pKa = 3.9RR265 pKa = 11.84PFGDD269 pKa = 3.4EE270 pKa = 3.86YY271 pKa = 11.41VRR273 pKa = 11.84MRR275 pKa = 11.84EE276 pKa = 3.97EE277 pKa = 4.49GNSLEE282 pKa = 4.03SCMAHH287 pKa = 6.42SAGNYY292 pKa = 7.28DD293 pKa = 3.56APFNVHH299 pKa = 6.32PCDD302 pKa = 3.89VYY304 pKa = 10.17SCAYY308 pKa = 9.62YY309 pKa = 10.55GQGDD313 pKa = 3.68NGLVLVEE320 pKa = 4.23AQQGGIPVGRR330 pKa = 11.84GILNVHH336 pKa = 6.29TKK338 pKa = 10.52QIVRR342 pKa = 11.84WYY344 pKa = 9.71GEE346 pKa = 3.93YY347 pKa = 10.12KK348 pKa = 10.59ASVMLKK354 pKa = 7.91NTFGIKK360 pKa = 9.92VDD362 pKa = 4.04GDD364 pKa = 3.86ALDD367 pKa = 4.81DD368 pKa = 3.83VQLALIQDD376 pKa = 4.12GAKK379 pKa = 9.78IAAPYY384 pKa = 10.71LDD386 pKa = 4.48GCQACDD392 pKa = 3.32IEE394 pKa = 6.07DD395 pKa = 3.82GEE397 pKa = 4.82LYY399 pKa = 10.36VRR401 pKa = 11.84HH402 pKa = 6.47RR403 pKa = 11.84GDD405 pKa = 2.9IVMDD409 pKa = 3.83DD410 pKa = 3.18TSGYY414 pKa = 8.7QDD416 pKa = 3.24CKK418 pKa = 10.97ARR420 pKa = 11.84EE421 pKa = 4.22LDD423 pKa = 3.87TLSGDD428 pKa = 4.1YY429 pKa = 10.82YY430 pKa = 10.47PEE432 pKa = 5.21SDD434 pKa = 5.27LEE436 pKa = 4.07YY437 pKa = 10.91QPIHH441 pKa = 6.05DD442 pKa = 4.27TYY444 pKa = 10.97YY445 pKa = 11.17NPDD448 pKa = 3.78NTHH451 pKa = 6.05MGYY454 pKa = 10.23FCPITEE460 pKa = 4.7EE461 pKa = 4.26YY462 pKa = 9.65CNPYY466 pKa = 8.45ATSAAYY472 pKa = 10.48VDD474 pKa = 4.51GEE476 pKa = 4.36VTRR479 pKa = 11.84ISDD482 pKa = 3.49VVYY485 pKa = 10.02FGYY488 pKa = 10.37INPPGSWEE496 pKa = 3.94NLGGSIGWTEE506 pKa = 3.6NSEE509 pKa = 4.27GYY511 pKa = 10.5SYY513 pKa = 11.55DD514 pKa = 3.93DD515 pKa = 4.06EE516 pKa = 4.56TEE518 pKa = 3.38EE519 pKa = 4.61WYY521 pKa = 9.7TNEE524 pKa = 5.4QYY526 pKa = 11.14DD527 pKa = 3.47QLIAEE532 pKa = 4.46RR533 pKa = 11.84EE534 pKa = 4.05EE535 pKa = 3.98QEE537 pKa = 3.91EE538 pKa = 4.46DD539 pKa = 3.31KK540 pKa = 11.71DD541 pKa = 3.78EE542 pKa = 4.28AAA544 pKa = 5.0

Molecular weight: 61.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219MH35|A0A219MH35_9CAUD Uncharacterized protein OS=Dickeya phage BF25/12 OX=1698708 GN=BF2512_51 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.0DD3 pKa = 3.49YY4 pKa = 11.73LEE6 pKa = 4.5VCSTSRR12 pKa = 11.84TGLRR16 pKa = 11.84WKK18 pKa = 9.98QRR20 pKa = 11.84PKK22 pKa = 10.46QSSVTPGEE30 pKa = 3.93PAFTSVNGKK39 pKa = 9.98GYY41 pKa = 10.5YY42 pKa = 9.56EE43 pKa = 5.15GYY45 pKa = 10.48FKK47 pKa = 11.03GKK49 pKa = 9.67RR50 pKa = 11.84YY51 pKa = 9.05LAHH54 pKa = 6.79RR55 pKa = 11.84VIVYY59 pKa = 9.6LLSGKK64 pKa = 9.05WPEE67 pKa = 4.22QVDD70 pKa = 4.22HH71 pKa = 7.29IDD73 pKa = 3.64GNRR76 pKa = 11.84SNNCPTNLRR85 pKa = 11.84AVTAAEE91 pKa = 3.96NQHH94 pKa = 6.04NKK96 pKa = 9.24LANGFKK102 pKa = 10.39LDD104 pKa = 3.82PRR106 pKa = 11.84VGRR109 pKa = 11.84YY110 pKa = 6.38QARR113 pKa = 11.84IRR115 pKa = 11.84VDD117 pKa = 3.45GKK119 pKa = 10.16QVNLGSFATKK129 pKa = 10.35QEE131 pKa = 3.71AQAAYY136 pKa = 9.74LAAKK140 pKa = 8.36RR141 pKa = 11.84TYY143 pKa = 10.51HH144 pKa = 5.38PTAPEE149 pKa = 3.68RR150 pKa = 11.84CYY152 pKa = 11.43VKK154 pKa = 10.86

Molecular weight: 17.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13224

46

1039

259.3

28.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.725 ± 0.434

1.195 ± 0.177

6.322 ± 0.236

5.203 ± 0.277

3.176 ± 0.172

7.698 ± 0.252

2.178 ± 0.225

4.545 ± 0.189

4.515 ± 0.296

8.719 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.178

4.583 ± 0.225

4.114 ± 0.198

4.537 ± 0.353

5.74 ± 0.262

6.178 ± 0.258

6.496 ± 0.439

7.35 ± 0.2

1.338 ± 0.112

3.675 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski