Prochlorococcus sp. HOT_208_60

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochloraceae; Prochlorococcus; unclassified Prochlorococcus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3K1R9|A0A1Y3K1R9_9PROC CDP-glucose 4 6-dehydratase (Fragment) OS=Prochlorococcus sp. HOT_208_60 OX=1945637 GN=B0W81_01520 PE=4 SV=1
MM1 pKa = 7.65ASYY4 pKa = 10.85KK5 pKa = 9.41VTLINEE11 pKa = 4.47GEE13 pKa = 4.38GLNSTIEE20 pKa = 4.21VPDD23 pKa = 3.7DD24 pKa = 3.94QYY26 pKa = 11.88ILDD29 pKa = 3.96AAEE32 pKa = 4.03EE33 pKa = 4.02QGIDD37 pKa = 3.69LPYY40 pKa = 10.01SCRR43 pKa = 11.84AGACSTCAGKK53 pKa = 8.27VTSGSVDD60 pKa = 3.2QSDD63 pKa = 4.14QSFLDD68 pKa = 4.03DD69 pKa = 4.44DD70 pKa = 3.69QLEE73 pKa = 4.34AGFVLTCVAYY83 pKa = 6.98PTSDD87 pKa = 3.36VTITTHH93 pKa = 8.14AEE95 pKa = 3.71EE96 pKa = 4.05EE97 pKa = 4.77LYY99 pKa = 11.03

Molecular weight: 10.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3K0V9|A0A1Y3K0V9_9PROC Uncharacterized protein OS=Prochlorococcus sp. HOT_208_60 OX=1945637 GN=B0W81_02295 PE=4 SV=1
MM1 pKa = 7.41TKK3 pKa = 9.05RR4 pKa = 11.84TFGGTSRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.25TGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.15SRR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 9.67GRR40 pKa = 11.84EE41 pKa = 3.79RR42 pKa = 11.84IAVV45 pKa = 3.46

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1097

0

1097

247331

22

1165

225.5

25.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.452 ± 0.08

1.148 ± 0.023

5.089 ± 0.059

6.709 ± 0.08

4.988 ± 0.076

6.366 ± 0.077

1.535 ± 0.042

9.058 ± 0.09

8.623 ± 0.096

10.702 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.83 ± 0.033

6.413 ± 0.077

3.61 ± 0.044

3.047 ± 0.039

3.884 ± 0.053

7.69 ± 0.067

4.454 ± 0.052

5.229 ± 0.065

1.259 ± 0.029

2.905 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski