Lewinella aurantiaca
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4341 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7G0F6|A0A5C7G0F6_9BACT Ligase-associated DNA damage response exonuclease OS=Lewinella aurantiaca OX=2602767 GN=FUA23_02695 PE=4 SV=1
MM1 pKa = 7.21 YY2 pKa = 10.31 KK3 pKa = 10.33 VNCLLLSTMLMLTLANASLLAQGTTYY29 pKa = 10.5 EE30 pKa = 4.28 VNFASIAFVNLEE42 pKa = 4.24 LNEE45 pKa = 4.46 DD46 pKa = 3.78 CTRR49 pKa = 11.84 DD50 pKa = 3.77 VIYY53 pKa = 10.73 MNLLTGNSDD62 pKa = 2.89 VDD64 pKa = 3.49 GDD66 pKa = 4.64 GIVPPQEE73 pKa = 3.69 AFIIEE78 pKa = 4.22 IDD80 pKa = 4.88 DD81 pKa = 3.94 NSPDD85 pKa = 3.48 NGPIVDD91 pKa = 3.95 GCGMFNYY98 pKa = 9.53 TIRR101 pKa = 11.84 PNPDD105 pKa = 2.66 SSVVGFTFGSGTITARR121 pKa = 11.84 DD122 pKa = 3.81 ATPPQQFGTATPNIGPFFTPQLQEE146 pKa = 3.83 LTINTLPASISRR158 pKa = 11.84 TFMVDD163 pKa = 3.03 GASGFPLMNSLSGEE177 pKa = 4.01 LMNRR181 pKa = 11.84 LLAGGIIPRR190 pKa = 11.84 FTDD193 pKa = 2.93 ACSDD197 pKa = 3.7 VTVVVSDD204 pKa = 4.27 EE205 pKa = 4.45 IINTGACEE213 pKa = 4.22 DD214 pKa = 3.76 IIIRR218 pKa = 11.84 RR219 pKa = 11.84 SFMATDD225 pKa = 4.1 ASADD229 pKa = 3.99 CISPNPAGNNGTTTVTYY246 pKa = 10.17 DD247 pKa = 3.15 IVLEE251 pKa = 4.37 RR252 pKa = 11.84 PNSANVIAPIDD263 pKa = 3.83 LVTYY267 pKa = 8.86 EE268 pKa = 4.8 CNDD271 pKa = 3.53 PALAGGNFPDD281 pKa = 5.49 PDD283 pKa = 4.69 PEE285 pKa = 5.52 DD286 pKa = 3.85 YY287 pKa = 11.0 PFLNGPNGPVYY298 pKa = 10.88 LNEE301 pKa = 3.86 IYY303 pKa = 11.09 GNVGASFTNSGAVQICNNTIKK324 pKa = 10.63 YY325 pKa = 8.82 VRR327 pKa = 11.84 TYY329 pKa = 10.4 TVIDD333 pKa = 3.28 WCDD336 pKa = 3.11 TDD338 pKa = 4.09 NVQTFTQLVKK348 pKa = 11.04 VGDD351 pKa = 3.86 TGAPTIVAPTQDD363 pKa = 4.31 LDD365 pKa = 4.39 FDD367 pKa = 5.08 LLPDD371 pKa = 4.66 DD372 pKa = 4.9 GPLVFSTNAPGCGAYY387 pKa = 8.78 LTTNMAGLSITDD399 pKa = 3.56 GCSALMTVTAFVLINGDD416 pKa = 3.47 EE417 pKa = 4.22 DD418 pKa = 4.39 NISGAINVYY427 pKa = 10.44 ASSATDD433 pKa = 3.07 RR434 pKa = 11.84 LTPFLPAGPHH444 pKa = 6.33 IIRR447 pKa = 11.84 YY448 pKa = 7.68 VAEE451 pKa = 4.6 DD452 pKa = 3.31 EE453 pKa = 5.09 CGNQTTSDD461 pKa = 3.59 LDD463 pKa = 3.42 IVVEE467 pKa = 4.21 DD468 pKa = 3.71 RR469 pKa = 11.84 SGPVMIVEE477 pKa = 4.55 DD478 pKa = 4.37 ALNVALSSSGFATVNATDD496 pKa = 4.59 LDD498 pKa = 4.0 EE499 pKa = 5.85 GSYY502 pKa = 11.3 DD503 pKa = 3.74 DD504 pKa = 4.68 CTDD507 pKa = 2.82 ITLEE511 pKa = 3.93 IAFANINSLMPIGAYY526 pKa = 10.02 GPSITLSCVDD536 pKa = 4.56 LDD538 pKa = 3.92 QVVTGIPVIIRR549 pKa = 11.84 GTDD552 pKa = 3.1 EE553 pKa = 4.18 NGNTNTRR560 pKa = 11.84 MSVLNVVDD568 pKa = 3.92 NTAPICIAPGNLNLTCSEE586 pKa = 4.22 ADD588 pKa = 3.02 AMLPEE593 pKa = 5.05 DD594 pKa = 3.93 VNAVYY599 pKa = 8.54 NTDD602 pKa = 3.5 PEE604 pKa = 4.69 GTVQMFDD611 pKa = 3.22 QLFGEE616 pKa = 4.69 VTTLDD621 pKa = 3.46 NCGNEE626 pKa = 3.83 YY627 pKa = 10.45 SAQNIVANINDD638 pKa = 4.71 CGTGTINRR646 pKa = 11.84 SFTVTDD652 pKa = 3.75 GQGFVSAPGCEE663 pKa = 4.01 QIISIRR669 pKa = 11.84 GVRR672 pKa = 11.84 NYY674 pKa = 9.02 TVEE677 pKa = 4.27 FPGDD681 pKa = 3.31 ASATCGLMPDD691 pKa = 3.81 VNDD694 pKa = 4.46 FVYY697 pKa = 10.55 TPLGCDD703 pKa = 2.93 MVVSYY708 pKa = 11.35 VEE710 pKa = 3.71 VDD712 pKa = 3.47 TFFAASDD719 pKa = 3.42 ACFKK723 pKa = 10.33 IRR725 pKa = 11.84 RR726 pKa = 11.84 TIEE729 pKa = 3.8 IINWCEE735 pKa = 3.29 YY736 pKa = 10.89 DD737 pKa = 3.77 GNGDD741 pKa = 4.07 FYY743 pKa = 10.5 TIRR746 pKa = 11.84 RR747 pKa = 11.84 DD748 pKa = 3.17 ADD750 pKa = 3.14 NDD752 pKa = 3.67 GNYY755 pKa = 10.29 EE756 pKa = 3.98 EE757 pKa = 4.97 STFLHH762 pKa = 6.61 VIPNGNTDD770 pKa = 3.31 AGDD773 pKa = 3.79 DD774 pKa = 4.1 VAVLDD779 pKa = 4.67 QDD781 pKa = 4.14 SDD783 pKa = 3.7 RR784 pKa = 11.84 DD785 pKa = 3.7 NLNNIGFLDD794 pKa = 3.53 VDD796 pKa = 4.41 DD797 pKa = 5.01 NFGAFGTDD805 pKa = 2.89 SDD807 pKa = 4.29 NDD809 pKa = 3.73 GDD811 pKa = 3.73 TGYY814 pKa = 11.37 ADD816 pKa = 3.56 SEE818 pKa = 4.46 SRR820 pKa = 11.84 GAFRR824 pKa = 11.84 YY825 pKa = 8.7 VQFIKK830 pKa = 10.8 VYY832 pKa = 10.97 DD833 pKa = 4.44 NIAPTITNISSDD845 pKa = 3.54 VANSEE850 pKa = 4.16 DD851 pKa = 4.28 CNGGGIQIDD860 pKa = 3.81 YY861 pKa = 9.02 TITDD865 pKa = 3.75 DD866 pKa = 4.76 CVGANLSTNVEE877 pKa = 3.83 LDD879 pKa = 3.61 LNFVAGGGFSATRR892 pKa = 11.84 EE893 pKa = 4.16 LTSDD897 pKa = 3.56 EE898 pKa = 4.63 VIGDD902 pKa = 3.49 GNGNYY907 pKa = 10.03 NVILSGLPVGEE918 pKa = 3.91 HH919 pKa = 7.52 AIRR922 pKa = 11.84 VAATDD927 pKa = 3.22 GCGNVNGRR935 pKa = 11.84 IIQFDD940 pKa = 3.4 IQDD943 pKa = 3.86 NSTVTPICIGRR954 pKa = 11.84 LTFVLMNDD962 pKa = 3.68 GAGGGSALVEE972 pKa = 4.01 ADD974 pKa = 3.96 DD975 pKa = 5.45 FVVSLNGNCNNVDD988 pKa = 3.01 VDD990 pKa = 3.46 YY991 pKa = 11.31 SIYY994 pKa = 10.41 RR995 pKa = 11.84 DD996 pKa = 3.78 FEE998 pKa = 4.75 YY999 pKa = 10.94 NDD1001 pKa = 3.78 PNFVPGTNRR1010 pKa = 11.84 TDD1012 pKa = 3.95 FPVSCDD1018 pKa = 3.46 DD1019 pKa = 3.65 VGDD1022 pKa = 4.04 ILVQVYY1028 pKa = 10.34 AFTPNGQAGLCNATAVIEE1046 pKa = 4.13 PSSTVSCTAANVASLSGFITSPRR1069 pKa = 11.84 NEE1071 pKa = 4.31 LLDD1074 pKa = 4.64 GIEE1077 pKa = 3.99 VHH1079 pKa = 6.53 ISDD1082 pKa = 5.01 MDD1084 pKa = 3.76 TMDD1087 pKa = 4.82 DD1088 pKa = 4.1 MLYY1091 pKa = 10.06 TDD1093 pKa = 4.71 ANGSFLFPALSEE1105 pKa = 3.81 GHH1107 pKa = 6.56 EE1108 pKa = 4.09 YY1109 pKa = 9.92 MIRR1112 pKa = 11.84 PSMPDD1117 pKa = 3.03 EE1118 pKa = 4.5 VNLEE1122 pKa = 4.23 RR1123 pKa = 11.84 IKK1125 pKa = 10.37 TSDD1128 pKa = 2.69 ITIIGAHH1135 pKa = 6.49 ALGAILIEE1143 pKa = 4.53 DD1144 pKa = 5.2 PYY1146 pKa = 11.88 RR1147 pKa = 11.84 MIAADD1152 pKa = 3.61 ANGDD1156 pKa = 3.73 GFIDD1160 pKa = 3.8 IGDD1163 pKa = 4.15 MIAIRR1168 pKa = 11.84 RR1169 pKa = 11.84 VILGIDD1175 pKa = 3.29 ATFTEE1180 pKa = 5.32 GPTWRR1185 pKa = 11.84 FIRR1188 pKa = 11.84 RR1189 pKa = 11.84 DD1190 pKa = 3.29 FDD1192 pKa = 4.94 LEE1194 pKa = 4.04 GLTEE1198 pKa = 4.28 GWDD1201 pKa = 3.3 PDD1203 pKa = 4.14 VFPTTYY1209 pKa = 10.0 RR1210 pKa = 11.84 VEE1212 pKa = 4.05 EE1213 pKa = 4.01 LLGHH1217 pKa = 5.75 NRR1219 pKa = 11.84 EE1220 pKa = 3.98 ADD1222 pKa = 3.87 FVAIEE1227 pKa = 4.06 IGDD1230 pKa = 3.86 VFVQSTGRR1238 pKa = 11.84 EE1239 pKa = 4.28 SQPLTASDD1247 pKa = 3.09 ATLAVGEE1254 pKa = 5.02 RR1255 pKa = 11.84 IDD1257 pKa = 5.11 LDD1259 pKa = 3.17 ITARR1263 pKa = 11.84 DD1264 pKa = 3.29 LAGFQGTFDD1273 pKa = 3.64 AASGLEE1279 pKa = 3.84 IEE1281 pKa = 4.81 GWSSEE1286 pKa = 4.07 VLSAGNVNDD1295 pKa = 4.43 RR1296 pKa = 11.84 YY1297 pKa = 10.65 LSHH1300 pKa = 6.85 GLLAVSYY1307 pKa = 10.77 NGDD1310 pKa = 3.78 EE1311 pKa = 4.78 LEE1313 pKa = 4.9 DD1314 pKa = 3.82 NAPVLTLHH1322 pKa = 6.66 LRR1324 pKa = 11.84 AAAPDD1329 pKa = 3.75 VRR1331 pKa = 11.84 ISDD1334 pKa = 4.05 YY1335 pKa = 11.51 LSVTDD1340 pKa = 4.18 RR1341 pKa = 11.84 MTYY1344 pKa = 10.19 PEE1346 pKa = 5.45 AILTGGNTATLSLEE1360 pKa = 4.26 FSEE1363 pKa = 4.82 TTAGGADD1370 pKa = 3.81 VILHH1374 pKa = 5.56 QNFPNPVAAQTNIVFEE1390 pKa = 4.57 LPRR1393 pKa = 11.84 ASDD1396 pKa = 3.5 VKK1398 pKa = 11.34 LEE1400 pKa = 3.92 VHH1402 pKa = 6.91 DD1403 pKa = 3.99 LQGRR1407 pKa = 11.84 IVTKK1411 pKa = 10.59 RR1412 pKa = 11.84 SLEE1415 pKa = 4.09 GQAGRR1420 pKa = 11.84 NTITLSTFDD1429 pKa = 4.98 DD1430 pKa = 4.15 LNNYY1434 pKa = 8.42 TGVLTYY1440 pKa = 10.03 TITVGQTRR1448 pKa = 11.84 LTKK1451 pKa = 10.67 RR1452 pKa = 11.84 MTVVAARR1459 pKa = 3.78
Molecular weight: 156.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.706
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.884
Patrickios 0.973
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A5C7F2H3|A0A5C7F2H3_9BACT DUF4221 domain-containing protein OS=Lewinella aurantiaca OX=2602767 GN=FUA23_21990 PE=4 SV=1
MM1 pKa = 6.98 TNHH4 pKa = 5.74 YY5 pKa = 7.38 RR6 pKa = 11.84 TCSRR10 pKa = 11.84 LASGGQRR17 pKa = 11.84 TASFPYY23 pKa = 9.84 RR24 pKa = 11.84 LAAVFFDD31 pKa = 3.5 VSRR34 pKa = 11.84 VSGVGLPTLLGICLLPLLLLCSGCGEE60 pKa = 4.5 KK61 pKa = 10.79 DD62 pKa = 3.06 PAADD66 pKa = 4.52 AEE68 pKa = 4.6 VTTMKK73 pKa = 10.72 ASAKK77 pKa = 9.08 PEE79 pKa = 3.85 PSAAEE84 pKa = 3.82 IPLVTLHH91 pKa = 5.99 TATRR95 pKa = 11.84 GRR97 pKa = 11.84 LPLRR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 ATGKK107 pKa = 9.95 LRR109 pKa = 11.84 ARR111 pKa = 11.84 RR112 pKa = 3.66
Molecular weight: 12.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.575
IPC_protein 10.496
Toseland 10.701
ProMoST 10.877
Dawson 10.774
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.877
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.73
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.657
IPC_peptide 10.95
IPC2_peptide 9.94
IPC2.peptide.svr19 8.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4341
0
4341
1692660
16
7041
389.9
43.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.458 ± 0.043
0.933 ± 0.023
6.226 ± 0.047
6.424 ± 0.034
4.607 ± 0.024
7.908 ± 0.039
1.786 ± 0.022
5.652 ± 0.029
4.411 ± 0.046
9.748 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.017
4.49 ± 0.03
4.578 ± 0.03
3.367 ± 0.024
5.35 ± 0.048
6.109 ± 0.03
6.17 ± 0.058
6.785 ± 0.033
1.247 ± 0.015
3.615 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here