Salmonella phage astrithr
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8R9B1|A0A6G8R9B1_9CAUD Peptidase C51 domain-containing protein OS=Salmonella phage astrithr OX=2713276 GN=astrithr_11 PE=4 SV=1
MM1 pKa = 6.67 ATVYY5 pKa = 10.53 RR6 pKa = 11.84 FTQADD11 pKa = 3.05 IDD13 pKa = 3.7 AAAAQGVTMKK23 pKa = 10.9 LSGLAVSDD31 pKa = 3.73 FDD33 pKa = 4.85 GFSDD37 pKa = 4.6 SDD39 pKa = 4.01 TLVATASGGRR49 pKa = 11.84 KK50 pKa = 7.7 FTTIPATAWEE60 pKa = 4.16 SAYY63 pKa = 9.93 TSIYY67 pKa = 9.79 FFGWDD72 pKa = 3.27 SVEE75 pKa = 4.15 GGNINIPFVLSNNDD89 pKa = 2.84 TVATLKK95 pKa = 10.25 PAAGMKK101 pKa = 9.83 FNKK104 pKa = 9.93 FYY106 pKa = 10.82 VTTQEE111 pKa = 3.94 AATALEE117 pKa = 4.38 WDD119 pKa = 3.74 NHH121 pKa = 5.78 PSSGSEE127 pKa = 3.67 NTDD130 pKa = 3.83 VVFTWDD136 pKa = 3.81 GGTLTTGNYY145 pKa = 9.52 NVVVTKK151 pKa = 10.79 DD152 pKa = 2.99 GAEE155 pKa = 3.85 IDD157 pKa = 4.51 NISTPDD163 pKa = 3.42 KK164 pKa = 11.05 SYY166 pKa = 9.65 TLNKK170 pKa = 9.23 PAGDD174 pKa = 3.96 YY175 pKa = 11.04 VVTVYY180 pKa = 11.22 DD181 pKa = 3.91 KK182 pKa = 11.42 GGKK185 pKa = 9.17 SDD187 pKa = 3.63 STASNIRR194 pKa = 11.84 QAIAVSAVLPKK205 pKa = 10.62 ALYY208 pKa = 8.21 TVKK211 pKa = 10.51 AADD214 pKa = 3.45 MTAISDD220 pKa = 3.8 NEE222 pKa = 3.44 IDD224 pKa = 3.77 MKK226 pKa = 11.67 VNGADD231 pKa = 2.89 ITTGSVLRR239 pKa = 11.84 LGDD242 pKa = 3.65 VIVAKK247 pKa = 10.85 VSGIRR252 pKa = 11.84 KK253 pKa = 9.29 FYY255 pKa = 10.85 TDD257 pKa = 3.33 TTLHH261 pKa = 5.37 GTSINFAVFFDD272 pKa = 5.2 GGMDD276 pKa = 3.39 WLQFTLSDD284 pKa = 3.64 NDD286 pKa = 3.34 QTATFTMVDD295 pKa = 3.79 DD296 pKa = 4.69 ASGSGGTYY304 pKa = 8.53 QAWNIRR310 pKa = 11.84 TKK312 pKa = 10.57 QEE314 pKa = 3.66 TPAVVGTNNVYY325 pKa = 10.68 KK326 pKa = 10.05 IDD328 pKa = 3.75 AAILSSVNKK337 pKa = 9.67 EE338 pKa = 3.73 RR339 pKa = 11.84 FVTITGSDD347 pKa = 3.41 SLFDD351 pKa = 3.63 YY352 pKa = 10.32 GQYY355 pKa = 10.15 ILSVLQFPFDD365 pKa = 3.39 IPANQILSPEE375 pKa = 4.45 NIQLANRR382 pKa = 11.84 QLSVSANKK390 pKa = 10.12 LATDD394 pKa = 4.83 KK395 pKa = 11.17 IKK397 pKa = 10.47 IDD399 pKa = 3.6 LGEE402 pKa = 4.55 IIVPDD407 pKa = 3.78 TYY409 pKa = 11.9 GNMLSYY415 pKa = 11.53 ANTNAVIHH423 pKa = 6.43 LPLAPSIVLDD433 pKa = 3.7 LEE435 pKa = 4.46 YY436 pKa = 11.1 VIGQTLGVYY445 pKa = 10.19 YY446 pKa = 10.95 LLDD449 pKa = 4.86 CYY451 pKa = 10.79 TGTATINITSTKK463 pKa = 8.71 LAAVISSTQVNIGVRR478 pKa = 11.84 VPYY481 pKa = 9.89 MADD484 pKa = 2.97 SYY486 pKa = 10.14 TAPEE490 pKa = 4.06 NTGVVAGGNNGVKK503 pKa = 9.69 IPYY506 pKa = 9.48 IEE508 pKa = 5.51 LISHH512 pKa = 7.26 DD513 pKa = 5.09 AILPHH518 pKa = 6.82 GFFTVPVVDD527 pKa = 3.68 EE528 pKa = 4.36 TLISGQTGYY537 pKa = 10.64 IKK539 pKa = 10.53 VDD541 pKa = 3.2 NVEE544 pKa = 4.44 LVTGALGNEE553 pKa = 4.02 KK554 pKa = 10.56 AQIISLLNSGVIIKK568 pKa = 10.32
Molecular weight: 60.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.356
IPC2_protein 4.355
IPC_protein 4.368
Toseland 4.151
ProMoST 4.507
Dawson 4.355
Bjellqvist 4.495
Wikipedia 4.279
Rodwell 4.19
Grimsley 4.062
Solomon 4.342
Lehninger 4.304
Nozaki 4.457
DTASelect 4.724
Thurlkill 4.19
EMBOSS 4.291
Sillero 4.482
Patrickios 3.554
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.419
Protein with the highest isoelectric point:
>tr|A0A6G8R963|A0A6G8R963_9CAUD Uncharacterized protein OS=Salmonella phage astrithr OX=2713276 GN=astrithr_7 PE=4 SV=1
MM1 pKa = 7.88 GINITRR7 pKa = 11.84 QRR9 pKa = 11.84 LLDD12 pKa = 3.53 GMRR15 pKa = 11.84 SIGEE19 pKa = 4.23 PFTQSEE25 pKa = 4.33 IEE27 pKa = 4.47 SICRR31 pKa = 11.84 EE32 pKa = 4.01 LFHH35 pKa = 7.08 NRR37 pKa = 11.84 GKK39 pKa = 10.51 ISDD42 pKa = 4.49 LKK44 pKa = 11.19 NKK46 pKa = 9.57 LASKK50 pKa = 10.39 SDD52 pKa = 3.5 NKK54 pKa = 10.32 KK55 pKa = 10.83 APP57 pKa = 3.43
Molecular weight: 6.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 8.902
IPC_protein 8.946
Toseland 10.145
ProMoST 9.531
Dawson 10.189
Bjellqvist 9.736
Wikipedia 10.233
Rodwell 10.847
Grimsley 10.175
Solomon 10.248
Lehninger 10.262
Nozaki 10.145
DTASelect 9.721
Thurlkill 10.087
EMBOSS 10.496
Sillero 10.101
Patrickios 10.716
IPC_peptide 10.262
IPC2_peptide 8.244
IPC2.peptide.svr19 7.987
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
3631
57
708
242.1
27.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.885 ± 0.703
0.771 ± 0.226
6.582 ± 0.419
6.527 ± 0.733
4.186 ± 0.278
6.472 ± 0.416
1.377 ± 0.281
6.858 ± 0.396
7.408 ± 0.705
7.711 ± 0.377
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.112 ± 0.311
6.72 ± 0.546
2.644 ± 0.234
3.195 ± 0.656
3.167 ± 0.38
6.445 ± 0.392
7.05 ± 0.735
7.078 ± 0.704
0.991 ± 0.146
4.82 ± 0.78
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here