Salmonella phage astrithr

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Astrithrvirus; Salmonella virus astrithr

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6G8R9B1|A0A6G8R9B1_9CAUD Peptidase C51 domain-containing protein OS=Salmonella phage astrithr OX=2713276 GN=astrithr_11 PE=4 SV=1
MM1 pKa = 6.67ATVYY5 pKa = 10.53RR6 pKa = 11.84FTQADD11 pKa = 3.05IDD13 pKa = 3.7AAAAQGVTMKK23 pKa = 10.9LSGLAVSDD31 pKa = 3.73FDD33 pKa = 4.85GFSDD37 pKa = 4.6SDD39 pKa = 4.01TLVATASGGRR49 pKa = 11.84KK50 pKa = 7.7FTTIPATAWEE60 pKa = 4.16SAYY63 pKa = 9.93TSIYY67 pKa = 9.79FFGWDD72 pKa = 3.27SVEE75 pKa = 4.15GGNINIPFVLSNNDD89 pKa = 2.84TVATLKK95 pKa = 10.25PAAGMKK101 pKa = 9.83FNKK104 pKa = 9.93FYY106 pKa = 10.82VTTQEE111 pKa = 3.94AATALEE117 pKa = 4.38WDD119 pKa = 3.74NHH121 pKa = 5.78PSSGSEE127 pKa = 3.67NTDD130 pKa = 3.83VVFTWDD136 pKa = 3.81GGTLTTGNYY145 pKa = 9.52NVVVTKK151 pKa = 10.79DD152 pKa = 2.99GAEE155 pKa = 3.85IDD157 pKa = 4.51NISTPDD163 pKa = 3.42KK164 pKa = 11.05SYY166 pKa = 9.65TLNKK170 pKa = 9.23PAGDD174 pKa = 3.96YY175 pKa = 11.04VVTVYY180 pKa = 11.22DD181 pKa = 3.91KK182 pKa = 11.42GGKK185 pKa = 9.17SDD187 pKa = 3.63STASNIRR194 pKa = 11.84QAIAVSAVLPKK205 pKa = 10.62ALYY208 pKa = 8.21TVKK211 pKa = 10.51AADD214 pKa = 3.45MTAISDD220 pKa = 3.8NEE222 pKa = 3.44IDD224 pKa = 3.77MKK226 pKa = 11.67VNGADD231 pKa = 2.89ITTGSVLRR239 pKa = 11.84LGDD242 pKa = 3.65VIVAKK247 pKa = 10.85VSGIRR252 pKa = 11.84KK253 pKa = 9.29FYY255 pKa = 10.85TDD257 pKa = 3.33TTLHH261 pKa = 5.37GTSINFAVFFDD272 pKa = 5.2GGMDD276 pKa = 3.39WLQFTLSDD284 pKa = 3.64NDD286 pKa = 3.34QTATFTMVDD295 pKa = 3.79DD296 pKa = 4.69ASGSGGTYY304 pKa = 8.53QAWNIRR310 pKa = 11.84TKK312 pKa = 10.57QEE314 pKa = 3.66TPAVVGTNNVYY325 pKa = 10.68KK326 pKa = 10.05IDD328 pKa = 3.75AAILSSVNKK337 pKa = 9.67EE338 pKa = 3.73RR339 pKa = 11.84FVTITGSDD347 pKa = 3.41SLFDD351 pKa = 3.63YY352 pKa = 10.32GQYY355 pKa = 10.15ILSVLQFPFDD365 pKa = 3.39IPANQILSPEE375 pKa = 4.45NIQLANRR382 pKa = 11.84QLSVSANKK390 pKa = 10.12LATDD394 pKa = 4.83KK395 pKa = 11.17IKK397 pKa = 10.47IDD399 pKa = 3.6LGEE402 pKa = 4.55IIVPDD407 pKa = 3.78TYY409 pKa = 11.9GNMLSYY415 pKa = 11.53ANTNAVIHH423 pKa = 6.43LPLAPSIVLDD433 pKa = 3.7LEE435 pKa = 4.46YY436 pKa = 11.1VIGQTLGVYY445 pKa = 10.19YY446 pKa = 10.95LLDD449 pKa = 4.86CYY451 pKa = 10.79TGTATINITSTKK463 pKa = 8.71LAAVISSTQVNIGVRR478 pKa = 11.84VPYY481 pKa = 9.89MADD484 pKa = 2.97SYY486 pKa = 10.14TAPEE490 pKa = 4.06NTGVVAGGNNGVKK503 pKa = 9.69IPYY506 pKa = 9.48IEE508 pKa = 5.51LISHH512 pKa = 7.26DD513 pKa = 5.09AILPHH518 pKa = 6.82GFFTVPVVDD527 pKa = 3.68EE528 pKa = 4.36TLISGQTGYY537 pKa = 10.64IKK539 pKa = 10.53VDD541 pKa = 3.2NVEE544 pKa = 4.44LVTGALGNEE553 pKa = 4.02KK554 pKa = 10.56AQIISLLNSGVIIKK568 pKa = 10.32

Molecular weight:
60.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6G8R963|A0A6G8R963_9CAUD Uncharacterized protein OS=Salmonella phage astrithr OX=2713276 GN=astrithr_7 PE=4 SV=1
MM1 pKa = 7.88GINITRR7 pKa = 11.84QRR9 pKa = 11.84LLDD12 pKa = 3.53GMRR15 pKa = 11.84SIGEE19 pKa = 4.23PFTQSEE25 pKa = 4.33IEE27 pKa = 4.47SICRR31 pKa = 11.84EE32 pKa = 4.01LFHH35 pKa = 7.08NRR37 pKa = 11.84GKK39 pKa = 10.51ISDD42 pKa = 4.49LKK44 pKa = 11.19NKK46 pKa = 9.57LASKK50 pKa = 10.39SDD52 pKa = 3.5NKK54 pKa = 10.32KK55 pKa = 10.83APP57 pKa = 3.43

Molecular weight:
6.47 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

3631

57

708

242.1

27.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.885 ± 0.703

0.771 ± 0.226

6.582 ± 0.419

6.527 ± 0.733

4.186 ± 0.278

6.472 ± 0.416

1.377 ± 0.281

6.858 ± 0.396

7.408 ± 0.705

7.711 ± 0.377

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.112 ± 0.311

6.72 ± 0.546

2.644 ± 0.234

3.195 ± 0.656

3.167 ± 0.38

6.445 ± 0.392

7.05 ± 0.735

7.078 ± 0.704

0.991 ± 0.146

4.82 ± 0.78

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski