Amino acid dipepetide frequency for Gordonia phage Bunnybear

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.879AlaAla: 16.879 ± 1.645
1.133AlaCys: 1.133 ± 0.317
8.589AlaAsp: 8.589 ± 0.718
7.217AlaGlu: 7.217 ± 0.55
2.207AlaPhe: 2.207 ± 0.378
9.782AlaGly: 9.782 ± 0.794
2.028AlaHis: 2.028 ± 0.353
4.95AlaIle: 4.95 ± 0.526
4.235AlaLys: 4.235 ± 0.546
8.291AlaLeu: 8.291 ± 0.862
3.817AlaMet: 3.817 ± 0.483
2.684AlaAsn: 2.684 ± 0.333
5.308AlaPro: 5.308 ± 0.538
4.235AlaGln: 4.235 ± 0.607
7.694AlaArg: 7.694 ± 0.607
6.084AlaSer: 6.084 ± 0.886
7.396AlaThr: 7.396 ± 1.021
8.529AlaVal: 8.529 ± 0.667
1.789AlaTrp: 1.789 ± 0.338
2.505AlaTyr: 2.505 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
1.253CysAla: 1.253 ± 0.371
0.239CysCys: 0.239 ± 0.137
0.716CysAsp: 0.716 ± 0.246
0.716CysGlu: 0.716 ± 0.217
0.06CysPhe: 0.06 ± 0.071
1.253CysGly: 1.253 ± 0.381
0.298CysHis: 0.298 ± 0.13
0.119CysIle: 0.119 ± 0.072
0.179CysLys: 0.179 ± 0.098
0.179CysLeu: 0.179 ± 0.099
0.179CysMet: 0.179 ± 0.099
0.239CysAsn: 0.239 ± 0.113
0.596CysPro: 0.596 ± 0.197
0.239CysGln: 0.239 ± 0.126
0.716CysArg: 0.716 ± 0.24
0.298CysSer: 0.298 ± 0.146
0.537CysThr: 0.537 ± 0.179
0.358CysVal: 0.358 ± 0.145
0.298CysTrp: 0.298 ± 0.139
0.358CysTyr: 0.358 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.561AspAla: 6.561 ± 0.584
0.596AspCys: 0.596 ± 0.207
7.456AspAsp: 7.456 ± 1.044
4.414AspGlu: 4.414 ± 0.537
1.909AspPhe: 1.909 ± 0.316
6.263AspGly: 6.263 ± 0.816
1.968AspHis: 1.968 ± 0.378
2.565AspIle: 2.565 ± 0.392
1.491AspLys: 1.491 ± 0.32
7.515AspLeu: 7.515 ± 0.661
1.014AspMet: 1.014 ± 0.185
1.968AspAsn: 1.968 ± 0.274
4.533AspPro: 4.533 ± 0.496
2.147AspGln: 2.147 ± 0.379
5.786AspArg: 5.786 ± 0.772
3.161AspSer: 3.161 ± 0.491
3.519AspThr: 3.519 ± 0.436
5.01AspVal: 5.01 ± 0.513
1.551AspTrp: 1.551 ± 0.313
1.73AspTyr: 1.73 ± 0.266
0.0AspXaa: 0.0 ± 0.0
Glu
6.621GluAla: 6.621 ± 0.801
0.537GluCys: 0.537 ± 0.213
3.042GluAsp: 3.042 ± 0.519
3.161GluGlu: 3.161 ± 0.451
1.909GluPhe: 1.909 ± 0.392
3.638GluGly: 3.638 ± 0.522
0.895GluHis: 0.895 ± 0.279
2.803GluIle: 2.803 ± 0.502
2.266GluLys: 2.266 ± 0.41
5.249GluLeu: 5.249 ± 0.585
1.074GluMet: 1.074 ± 0.282
1.312GluAsn: 1.312 ± 0.317
2.803GluPro: 2.803 ± 0.456
2.982GluGln: 2.982 ± 0.46
5.308GluArg: 5.308 ± 0.605
2.207GluSer: 2.207 ± 0.354
3.937GluThr: 3.937 ± 0.399
4.294GluVal: 4.294 ± 0.444
1.312GluTrp: 1.312 ± 0.291
1.133GluTyr: 1.133 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
2.803PheAla: 2.803 ± 0.411
0.119PheCys: 0.119 ± 0.08
2.386PheAsp: 2.386 ± 0.361
1.849PheGlu: 1.849 ± 0.306
0.716PhePhe: 0.716 ± 0.166
2.445PheGly: 2.445 ± 0.401
0.656PheHis: 0.656 ± 0.178
1.014PheIle: 1.014 ± 0.242
1.014PheLys: 1.014 ± 0.247
1.431PheLeu: 1.431 ± 0.361
0.298PheMet: 0.298 ± 0.124
0.954PheAsn: 0.954 ± 0.198
1.789PhePro: 1.789 ± 0.314
0.596PheGln: 0.596 ± 0.178
2.147PheArg: 2.147 ± 0.407
1.312PheSer: 1.312 ± 0.259
1.789PheThr: 1.789 ± 0.376
1.968PheVal: 1.968 ± 0.39
0.656PheTrp: 0.656 ± 0.177
0.477PheTyr: 0.477 ± 0.162
0.0PheXaa: 0.0 ± 0.0
Gly
7.634GlyAla: 7.634 ± 0.802
0.775GlyCys: 0.775 ± 0.324
5.368GlyAsp: 5.368 ± 0.583
4.115GlyGlu: 4.115 ± 0.398
2.445GlyPhe: 2.445 ± 0.342
9.066GlyGly: 9.066 ± 1.216
1.431GlyHis: 1.431 ± 0.338
4.294GlyIle: 4.294 ± 0.442
3.042GlyLys: 3.042 ± 0.408
6.143GlyLeu: 6.143 ± 0.682
1.849GlyMet: 1.849 ± 0.331
2.803GlyAsn: 2.803 ± 0.394
5.01GlyPro: 5.01 ± 0.408
3.638GlyGln: 3.638 ± 0.444
6.978GlyArg: 6.978 ± 0.657
4.593GlySer: 4.593 ± 0.505
6.203GlyThr: 6.203 ± 0.943
6.143GlyVal: 6.143 ± 0.629
1.431GlyTrp: 1.431 ± 0.245
2.624GlyTyr: 2.624 ± 0.342
0.0GlyXaa: 0.0 ± 0.0
His
2.028HisAla: 2.028 ± 0.32
0.239HisCys: 0.239 ± 0.105
1.253HisAsp: 1.253 ± 0.322
1.372HisGlu: 1.372 ± 0.362
0.358HisPhe: 0.358 ± 0.143
1.789HisGly: 1.789 ± 0.289
0.954HisHis: 0.954 ± 0.24
0.835HisIle: 0.835 ± 0.24
0.477HisLys: 0.477 ± 0.174
1.909HisLeu: 1.909 ± 0.403
0.239HisMet: 0.239 ± 0.111
0.358HisAsn: 0.358 ± 0.136
2.147HisPro: 2.147 ± 0.457
0.716HisGln: 0.716 ± 0.23
1.551HisArg: 1.551 ± 0.314
0.775HisSer: 0.775 ± 0.207
1.849HisThr: 1.849 ± 0.299
1.014HisVal: 1.014 ± 0.247
0.298HisTrp: 0.298 ± 0.143
1.014HisTyr: 1.014 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.845IleAla: 5.845 ± 0.53
0.358IleCys: 0.358 ± 0.128
4.95IleAsp: 4.95 ± 0.559
3.34IleGlu: 3.34 ± 0.459
0.477IlePhe: 0.477 ± 0.156
4.235IleGly: 4.235 ± 0.642
1.133IleHis: 1.133 ± 0.238
1.61IleIle: 1.61 ± 0.289
0.835IleLys: 0.835 ± 0.19
2.088IleLeu: 2.088 ± 0.304
0.418IleMet: 0.418 ± 0.139
1.431IleAsn: 1.431 ± 0.293
2.923IlePro: 2.923 ± 0.367
2.028IleGln: 2.028 ± 0.316
3.34IleArg: 3.34 ± 0.416
2.088IleSer: 2.088 ± 0.32
2.803IleThr: 2.803 ± 0.443
3.579IleVal: 3.579 ± 0.393
0.358IleTrp: 0.358 ± 0.148
1.193IleTyr: 1.193 ± 0.234
0.0IleXaa: 0.0 ± 0.0
Lys
3.996LysAla: 3.996 ± 0.724
0.239LysCys: 0.239 ± 0.099
1.67LysAsp: 1.67 ± 0.321
1.551LysGlu: 1.551 ± 0.377
1.133LysPhe: 1.133 ± 0.27
2.326LysGly: 2.326 ± 0.348
0.716LysHis: 0.716 ± 0.169
1.73LysIle: 1.73 ± 0.335
1.133LysLys: 1.133 ± 0.282
2.923LysLeu: 2.923 ± 0.382
0.418LysMet: 0.418 ± 0.196
0.775LysAsn: 0.775 ± 0.242
2.445LysPro: 2.445 ± 0.338
1.193LysGln: 1.193 ± 0.301
2.147LysArg: 2.147 ± 0.362
1.73LysSer: 1.73 ± 0.272
1.968LysThr: 1.968 ± 0.367
2.505LysVal: 2.505 ± 0.423
0.537LysTrp: 0.537 ± 0.179
0.835LysTyr: 0.835 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
10.617LeuAla: 10.617 ± 0.812
0.656LeuCys: 0.656 ± 0.202
5.428LeuAsp: 5.428 ± 0.618
4.115LeuGlu: 4.115 ± 0.649
2.803LeuPhe: 2.803 ± 0.538
5.129LeuGly: 5.129 ± 0.48
1.372LeuHis: 1.372 ± 0.377
3.519LeuIle: 3.519 ± 0.457
2.326LeuLys: 2.326 ± 0.346
5.428LeuLeu: 5.428 ± 0.554
1.67LeuMet: 1.67 ± 0.348
2.266LeuAsn: 2.266 ± 0.347
4.354LeuPro: 4.354 ± 0.476
2.147LeuGln: 2.147 ± 0.364
6.74LeuArg: 6.74 ± 0.756
4.473LeuSer: 4.473 ± 0.458
6.143LeuThr: 6.143 ± 0.656
5.129LeuVal: 5.129 ± 0.698
1.431LeuTrp: 1.431 ± 0.303
0.835LeuTyr: 0.835 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
2.744MetAla: 2.744 ± 0.465
0.239MetCys: 0.239 ± 0.112
0.596MetAsp: 0.596 ± 0.173
0.596MetGlu: 0.596 ± 0.21
0.239MetPhe: 0.239 ± 0.124
1.73MetGly: 1.73 ± 0.39
0.596MetHis: 0.596 ± 0.157
0.596MetIle: 0.596 ± 0.215
0.656MetLys: 0.656 ± 0.218
1.551MetLeu: 1.551 ± 0.336
0.239MetMet: 0.239 ± 0.146
0.835MetAsn: 0.835 ± 0.244
1.133MetPro: 1.133 ± 0.243
0.835MetGln: 0.835 ± 0.248
2.088MetArg: 2.088 ± 0.349
1.789MetSer: 1.789 ± 0.298
3.161MetThr: 3.161 ± 0.401
0.954MetVal: 0.954 ± 0.222
0.537MetTrp: 0.537 ± 0.227
0.179MetTyr: 0.179 ± 0.086
0.0MetXaa: 0.0 ± 0.0
Asn
2.803AsnAla: 2.803 ± 0.455
0.06AsnCys: 0.06 ± 0.069
2.088AsnAsp: 2.088 ± 0.367
0.954AsnGlu: 0.954 ± 0.264
0.418AsnPhe: 0.418 ± 0.165
3.221AsnGly: 3.221 ± 0.386
1.253AsnHis: 1.253 ± 0.333
1.014AsnIle: 1.014 ± 0.227
0.716AsnLys: 0.716 ± 0.238
1.849AsnLeu: 1.849 ± 0.299
0.418AsnMet: 0.418 ± 0.136
0.954AsnAsn: 0.954 ± 0.26
2.386AsnPro: 2.386 ± 0.352
0.775AsnGln: 0.775 ± 0.216
2.565AsnArg: 2.565 ± 0.413
2.028AsnSer: 2.028 ± 0.288
1.551AsnThr: 1.551 ± 0.317
1.73AsnVal: 1.73 ± 0.326
0.716AsnTrp: 0.716 ± 0.16
0.835AsnTyr: 0.835 ± 0.196
0.0AsnXaa: 0.0 ± 0.0
Pro
6.919ProAla: 6.919 ± 0.677
0.358ProCys: 0.358 ± 0.146
4.831ProAsp: 4.831 ± 0.722
4.294ProGlu: 4.294 ± 0.496
1.312ProPhe: 1.312 ± 0.214
6.143ProGly: 6.143 ± 0.596
0.895ProHis: 0.895 ± 0.233
2.565ProIle: 2.565 ± 0.386
1.849ProLys: 1.849 ± 0.335
3.877ProLeu: 3.877 ± 0.448
1.253ProMet: 1.253 ± 0.271
2.266ProAsn: 2.266 ± 0.337
3.042ProPro: 3.042 ± 0.472
1.312ProGln: 1.312 ± 0.247
4.294ProArg: 4.294 ± 0.619
3.459ProSer: 3.459 ± 0.314
4.414ProThr: 4.414 ± 0.541
4.175ProVal: 4.175 ± 0.423
1.312ProTrp: 1.312 ± 0.282
1.074ProTyr: 1.074 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
3.519GlnAla: 3.519 ± 0.51
0.358GlnCys: 0.358 ± 0.135
1.551GlnAsp: 1.551 ± 0.32
1.551GlnGlu: 1.551 ± 0.281
0.775GlnPhe: 0.775 ± 0.181
2.207GlnGly: 2.207 ± 0.533
0.954GlnHis: 0.954 ± 0.197
2.207GlnIle: 2.207 ± 0.397
1.67GlnLys: 1.67 ± 0.336
4.175GlnLeu: 4.175 ± 0.529
0.656GlnMet: 0.656 ± 0.17
0.954GlnAsn: 0.954 ± 0.203
1.909GlnPro: 1.909 ± 0.329
1.372GlnGln: 1.372 ± 0.326
3.579GlnArg: 3.579 ± 0.477
1.909GlnSer: 1.909 ± 0.44
1.849GlnThr: 1.849 ± 0.382
2.744GlnVal: 2.744 ± 0.375
0.656GlnTrp: 0.656 ± 0.172
0.716GlnTyr: 0.716 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
8.648ArgAla: 8.648 ± 0.822
0.596ArgCys: 0.596 ± 0.213
5.01ArgAsp: 5.01 ± 0.586
4.593ArgGlu: 4.593 ± 0.566
2.028ArgPhe: 2.028 ± 0.273
4.414ArgGly: 4.414 ± 0.511
1.312ArgHis: 1.312 ± 0.285
3.877ArgIle: 3.877 ± 0.655
3.4ArgLys: 3.4 ± 0.425
6.561ArgLeu: 6.561 ± 0.617
2.863ArgMet: 2.863 ± 0.366
2.266ArgAsn: 2.266 ± 0.333
4.772ArgPro: 4.772 ± 0.746
2.565ArgGln: 2.565 ± 0.461
5.905ArgArg: 5.905 ± 0.741
4.056ArgSer: 4.056 ± 0.434
4.95ArgThr: 4.95 ± 0.604
5.726ArgVal: 5.726 ± 0.521
2.088ArgTrp: 2.088 ± 0.411
2.505ArgTyr: 2.505 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
5.308SerAla: 5.308 ± 0.542
0.179SerCys: 0.179 ± 0.091
3.221SerAsp: 3.221 ± 0.388
3.28SerGlu: 3.28 ± 0.39
1.491SerPhe: 1.491 ± 0.26
5.786SerGly: 5.786 ± 0.697
0.537SerHis: 0.537 ± 0.147
2.624SerIle: 2.624 ± 0.517
1.909SerLys: 1.909 ± 0.329
3.817SerLeu: 3.817 ± 0.467
1.372SerMet: 1.372 ± 0.314
2.088SerAsn: 2.088 ± 0.359
2.863SerPro: 2.863 ± 0.374
2.266SerGln: 2.266 ± 0.529
2.982SerArg: 2.982 ± 0.4
2.326SerSer: 2.326 ± 0.448
3.758SerThr: 3.758 ± 0.433
4.354SerVal: 4.354 ± 0.597
1.133SerTrp: 1.133 ± 0.266
1.014SerTyr: 1.014 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
9.543ThrAla: 9.543 ± 1.117
0.537ThrCys: 0.537 ± 0.17
5.189ThrAsp: 5.189 ± 0.572
3.161ThrGlu: 3.161 ± 0.437
2.386ThrPhe: 2.386 ± 0.331
6.561ThrGly: 6.561 ± 0.766
1.67ThrHis: 1.67 ± 0.313
3.698ThrIle: 3.698 ± 0.419
1.61ThrLys: 1.61 ± 0.333
5.547ThrLeu: 5.547 ± 0.556
1.193ThrMet: 1.193 ± 0.258
1.312ThrAsn: 1.312 ± 0.33
4.533ThrPro: 4.533 ± 0.518
1.909ThrGln: 1.909 ± 0.329
4.593ThrArg: 4.593 ± 0.602
3.698ThrSer: 3.698 ± 0.471
6.024ThrThr: 6.024 ± 0.685
5.845ThrVal: 5.845 ± 0.568
1.014ThrTrp: 1.014 ± 0.277
1.431ThrTyr: 1.431 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
7.038ValAla: 7.038 ± 0.685
0.954ValCys: 0.954 ± 0.259
5.249ValAsp: 5.249 ± 0.499
4.235ValGlu: 4.235 ± 0.439
2.386ValPhe: 2.386 ± 0.423
6.143ValGly: 6.143 ± 0.626
1.491ValHis: 1.491 ± 0.258
3.937ValIle: 3.937 ± 0.44
2.028ValLys: 2.028 ± 0.419
4.831ValLeu: 4.831 ± 0.564
1.133ValMet: 1.133 ± 0.2
2.088ValAsn: 2.088 ± 0.32
4.652ValPro: 4.652 ± 0.469
2.565ValGln: 2.565 ± 0.697
5.905ValArg: 5.905 ± 0.584
3.937ValSer: 3.937 ± 0.528
6.382ValThr: 6.382 ± 0.751
6.322ValVal: 6.322 ± 0.712
0.835ValTrp: 0.835 ± 0.2
1.253ValTyr: 1.253 ± 0.253
0.0ValXaa: 0.0 ± 0.0
Trp
1.61TrpAla: 1.61 ± 0.29
0.358TrpCys: 0.358 ± 0.172
1.074TrpAsp: 1.074 ± 0.245
1.133TrpGlu: 1.133 ± 0.291
0.596TrpPhe: 0.596 ± 0.179
1.193TrpGly: 1.193 ± 0.218
0.537TrpHis: 0.537 ± 0.209
0.477TrpIle: 0.477 ± 0.156
0.775TrpLys: 0.775 ± 0.218
1.73TrpLeu: 1.73 ± 0.34
0.656TrpMet: 0.656 ± 0.215
0.477TrpAsn: 0.477 ± 0.155
1.074TrpPro: 1.074 ± 0.255
0.895TrpGln: 0.895 ± 0.242
1.789TrpArg: 1.789 ± 0.387
0.954TrpSer: 0.954 ± 0.244
1.551TrpThr: 1.551 ± 0.259
1.074TrpVal: 1.074 ± 0.284
0.418TrpTrp: 0.418 ± 0.127
0.537TrpTyr: 0.537 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.982TyrAla: 2.982 ± 0.443
0.298TyrCys: 0.298 ± 0.148
1.491TyrAsp: 1.491 ± 0.286
0.835TyrGlu: 0.835 ± 0.24
0.775TyrPhe: 0.775 ± 0.212
2.266TyrGly: 2.266 ± 0.348
0.477TyrHis: 0.477 ± 0.227
0.596TyrIle: 0.596 ± 0.196
0.358TyrLys: 0.358 ± 0.219
1.491TyrLeu: 1.491 ± 0.31
0.477TyrMet: 0.477 ± 0.148
0.298TyrAsn: 0.298 ± 0.135
1.372TyrPro: 1.372 ± 0.284
0.895TyrGln: 0.895 ± 0.208
2.088TyrArg: 2.088 ± 0.423
1.431TyrSer: 1.431 ± 0.247
1.67TyrThr: 1.67 ± 0.28
1.909TyrVal: 1.909 ± 0.293
0.537TyrTrp: 0.537 ± 0.186
0.418TyrTyr: 0.418 ± 0.142
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (16767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski