Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; Micromonospora aurantiaca

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9TAI3|D9TAI3_MICAI Uncharacterized protein OS=Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442) OX=644283 GN=Micau_4930 PE=4 SV=1
MM1 pKa = 7.28SHH3 pKa = 6.96RR4 pKa = 11.84FLSTSRR10 pKa = 11.84AAASVVGALVLAAAGLAGGAAPASTAYY37 pKa = 9.38IVPADD42 pKa = 3.88CGPYY46 pKa = 10.13SGAPVPAGYY55 pKa = 7.94TLRR58 pKa = 11.84FAGALYY64 pKa = 10.65VSNNANGPEE73 pKa = 4.31FIIGTTTRR81 pKa = 11.84NVIVGSVYY89 pKa = 11.14DD90 pKa = 5.6DD91 pKa = 4.79IICGLDD97 pKa = 3.44GNDD100 pKa = 5.15DD101 pKa = 3.49IQANSGDD108 pKa = 3.96DD109 pKa = 3.19IVYY112 pKa = 10.75GGLTGDD118 pKa = 5.58DD119 pKa = 2.85IWGDD123 pKa = 3.57LGNDD127 pKa = 3.48NLHH130 pKa = 6.93GDD132 pKa = 3.89GGADD136 pKa = 3.62TIHH139 pKa = 7.41GDD141 pKa = 3.58RR142 pKa = 11.84FIGPSALDD150 pKa = 3.45GNDD153 pKa = 3.2RR154 pKa = 11.84VDD156 pKa = 3.73GGSNDD161 pKa = 3.25DD162 pKa = 4.76HH163 pKa = 8.53LYY165 pKa = 11.0GDD167 pKa = 3.75QGVDD171 pKa = 3.42TLVGGPGWDD180 pKa = 5.23DD181 pKa = 3.71GDD183 pKa = 4.63CGPDD187 pKa = 3.67AVIDD191 pKa = 4.17DD192 pKa = 4.57PRR194 pKa = 11.84PTLDD198 pKa = 5.2LPINCPP204 pKa = 3.42

Molecular weight: 20.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9SYN7|D9SYN7_MICAI Uncharacterized protein OS=Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442) OX=644283 GN=Micau_1514 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6204

0

6204

2088683

30

9936

336.7

35.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.294 ± 0.057

0.74 ± 0.008

6.187 ± 0.031

5.089 ± 0.029

2.609 ± 0.018

9.466 ± 0.033

2.131 ± 0.015

2.96 ± 0.026

1.592 ± 0.022

10.53 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.598 ± 0.012

1.698 ± 0.019

6.494 ± 0.031

2.594 ± 0.018

8.637 ± 0.033

4.544 ± 0.024

6.079 ± 0.031

9.096 ± 0.031

1.624 ± 0.014

2.037 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski